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Q9JM52

- MINK1_MOUSE

UniProt

Q9JM52 - MINK1_MOUSE

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Protein

Misshapen-like kinase 1

Gene

Mink1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking. Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons. Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates: TANC1 upon stimulation by RAP2A, MBP and SMAD1. Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Magnesium.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541ATPPROSITE-ProRule annotation
Active sitei153 – 1531Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. protein serine/threonine kinase activity Source: UniProtKB
  3. small GTPase regulator activity Source: InterPro

GO - Biological processi

  1. dendrite morphogenesis Source: UniProtKB
  2. intracellular signal transduction Source: UniProtKB
  3. multicellular organismal development Source: UniProtKB
  4. negative thymic T cell selection Source: MGI
  5. protein autophosphorylation Source: UniProtKB
  6. protein phosphorylation Source: UniProtKB
  7. regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: UniProtKB
  8. response to stress Source: UniProtKB
  9. synaptic transmission Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_188970. Oxidative Stress Induced Senescence.

Names & Taxonomyi

Protein namesi
Recommended name:
Misshapen-like kinase 1 (EC:2.7.11.1)
Alternative name(s):
GCK family kinase MiNK
MAPK/ERK kinase kinase kinase 6
Short name:
MEK kinase kinase 6
Short name:
MEKKK 6
Misshapen/NIK-related kinase
Mitogen-activated protein kinase kinase kinase kinase 6
Gene namesi
Name:Mink1By similarity
Synonyms:Map4k6Imported, MinkImported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1355329. Mink1.

Subcellular locationi

Cytoplasm By similarity. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density By similarity. Cell projectionaxon By similarity. Cell projectiondendrite By similarity

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cell projection Source: UniProtKB-KW
  3. cytoplasm Source: UniProtKB
  4. postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13081308Misshapen-like kinase 1PRO_0000086330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei644 – 6441PhosphoserineBy similarity
Modified residuei729 – 7291Phosphoserine1 Publication
Modified residuei745 – 7451PhosphoserineBy similarity
Modified residuei746 – 7461PhosphoserineBy similarity
Modified residuei750 – 7501PhosphoserineBy similarity

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9JM52.
PaxDbiQ9JM52.
PRIDEiQ9JM52.

PTM databases

PhosphoSiteiQ9JM52.

Expressioni

Tissue specificityi

Appears to be ubiquitous, expressed in all tissue types examined. Highly expressed in the brain, moderately expressed in kidney and spleen, low levels present in heart and skeletal muscle. Isoform 2 is more abundant in the brain than isoform 1.1 Publication

Developmental stagei

Up-regulated during postnatal brain development.1 Publication

Gene expression databases

BgeeiQ9JM52.
CleanExiMM_MINK1.
ExpressionAtlasiQ9JM52. baseline and differential.
GenevestigatoriQ9JM52.

Interactioni

Subunit structurei

Interacts with RAP2A and TANC1 (By similarity). Interacts with NCK1.By similarity1 Publication

Protein-protein interaction databases

IntActiQ9JM52. 4 interactions.
MINTiMINT-1616244.

Structurei

3D structure databases

ProteinModelPortaliQ9JM52.
SMRiQ9JM52. Positions 12-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 289265Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini995 – 1282288CNHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni842 – 1308467Mediates interaction with RAP2ABy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi359 – 497139Gln-richAdd
BLAST
Compositional biasi544 – 672129Pro-richAdd
BLAST
Compositional biasi800 – 8034Poly-Ser
Compositional biasi810 – 8156Poly-Glu

Sequence similaritiesi

Contains 1 CNH domain.CuratedPROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118848.
HOGENOMiHOG000290708.
HOVERGENiHBG036506.
InParanoidiQ9JM52.
KOiK04413.

Family and domain databases

InterProiIPR001180. Citron.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00780. CNH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00036. CNH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50219. CNH. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 21 Publication (identifier: Q9JM52-1) [UniParc]FASTAAdd to Basket

Also known as: MiNK-2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDPAPARSL DDIDLSALRD PAGIFELVEV VGNGTYGQVY KGRHVKTGQL
60 70 80 90 100
AAIKVMDVTE DEEEEIKQEI NMLKKYSHHR NIATYYGAFI KKSPPGNDDQ
110 120 130 140 150
LWLVMEFCGA GSVTDLVKNT KGNALKEDCI AYICREILRG LAHLHAHKVI
160 170 180 190 200
HRDIKGQNVL LTENAEVKLV DFGVSAQLDR TVGRRNTFIG TPYWMAPEVI
210 220 230 240 250
ACDENPDATY DYRSDIWSLG ITAIEMAEGA PPLCDMHPMR ALFLIPRNPP
260 270 280 290 300
PRLKSKKWSK KFTDFIDTCL IKTYLSRPPT EQLLKFPFIR DQPTERQVRI
310 320 330 340 350
QLKDHIDRSR KKRGEKEETE YEYSGSEEED DSHGEEGEPS SIMNVPGEST
360 370 380 390 400
LRREFLRLQQ ENKSNSEALK QQQQLQQQQQ RDPEAHIKHL LHQRQRRIEE
410 420 430 440 450
QKEERRRVEE QQRREREQRK LQEKEQQRRL EDMQALRREE ERRQAEREQE
460 470 480 490 500
YKRKQLEEQR QSERLQRQLQ QEHAYLKSLQ QQQQQQQLQK QQQQQQQILP
510 520 530 540 550
GDRKPLYHYG RGINPADKPA WAREVEERAR MNKQQNSPLA KAKPSSAGPE
560 570 580 590 600
PPISQASPSP PGPLSQTPPM QRPVEPQEGP HKSLVAHRVP LKPYAAPVPR
610 620 630 640 650
SQSLQDQPTR NLAAFPASHD PDPAAVPTPT ATPSARGAVI RQNSDPTSEG
660 670 680 690 700
PGPSPNPPSW VRPDNEAPPK VPQRTSSIAT ALNTSGAGGS RPAQAVRASN
710 720 730 740 750
PDLRRSDPGW ERSDSVLPAS HGHLPQAGSL ERNRNRVGAS TKLDSSPVLS
760 770 780 790 800
PGNKAKPEDH RSRPGRPASY KRAIGEDFVL LKERTLDEAP KPPKKAMDYS
810 820 830 840 850
SSSEEVESSE EEEEEGDGEP SEGSRDTPGG RSDGDTDSVS TMVVHDVEEI
860 870 880 890 900
SGTQPSYGGG TMVVQRTPEE ERSLLLADSN GYTNLPDVVQ PSHSPTENSK
910 920 930 940 950
GQSPPTKDGG SDYQSRGLVK APGKSSFTMF VDLGIYQPGG SGDTIPITAL
960 970 980 990 1000
VGGEGGRLDQ LQFDVRKGSV VNVNPTNTRA HSETPEIRKY KKRFNSEILC
1010 1020 1030 1040 1050
AALWGVNLLV GTENGLMLLD RSGQGKVYGL IGRRRFQQMD VLEGLNLLIT
1060 1070 1080 1090 1100
ISGKRNKLRV YYLSWLRNKI LHNDPEVEKK QGWTTVGDME GCGHYRVVKY
1110 1120 1130 1140 1150
ERIKFLVIAL KNSVEVYAWA PKPYHKFMAF KSFADLPHRP LLVDLTVEEG
1160 1170 1180 1190 1200
QRLKVIYGSS AGFHAVDVDS GNSYDIYIPV HIQSQITPHA IIFLPNTDGM
1210 1220 1230 1240 1250
EMLLCYEDEG VYVNTYGRII KDVVLQWGEM PTSVAYICSN QIMGWGEKAI
1260 1270 1280 1290 1300
EIRSVETGHL DGVFMHKRAQ RLKFLCERND KVFFASVRSG GSSQVYFMTL

NRNCIMNW
Length:1,308
Mass (Da):147,295
Last modified:July 27, 2011 - v3
Checksum:i455E546EC5459B47
GO
Isoform 11 Publication (identifier: Q9JM52-2) [UniParc]FASTAAdd to Basket

Also known as: MiNK-1

The sequence of this isoform differs from the canonical sequence as follows:
     769-776: Missing.

Show »
Length:1,300
Mass (Da):146,390
Checksum:i9DB699C6D1F39C54
GO
Isoform 31 Publication (identifier: Q9JM52-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     698-698: A → ARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNAS

Note: No experimental confirmation available.

Show »
Length:1,345
Mass (Da):151,310
Checksum:i0A23B48F645B9258
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti221 – 2211I → V in AAH11346. (PubMed:15489334)Curated
Sequence conflicti243 – 2431F → L in AAH11346. (PubMed:15489334)Curated
Sequence conflicti317 – 3171E → R in AAH11346. (PubMed:15489334)Curated
Sequence conflicti542 – 5465AKPSS → RSQAG in BAA90752. (PubMed:10708748)Curated
Sequence conflicti542 – 5465AKPSS → RSQAG in BAA94837. (PubMed:10708748)Curated
Sequence conflicti832 – 8321Missing in AAH11346. (PubMed:15489334)Curated
Sequence conflicti840 – 8401S → T in BAA94837. (PubMed:10708748)Curated
Sequence conflicti840 – 8401S → T in BAA90752. (PubMed:10708748)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei698 – 6981A → ARPRSNSAWQIYLQRRAERG TPKPPGPPAQPPGPPNAS in isoform 3. 1 PublicationVSP_007061
Alternative sequencei769 – 7768Missing in isoform 1. 1 PublicationVSP_007062

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB041925 mRNA. Translation: BAA94837.1.
AB035697 mRNA. Translation: BAA90752.1.
AL592547 Genomic DNA. Translation: CAI24010.1.
CR933736 Genomic DNA. Translation: CAM28122.1.
CH466596 Genomic DNA. Translation: EDL12579.1.
BC011346 mRNA. Translation: AAH11346.1.
CCDSiCCDS24954.1. [Q9JM52-2]
CCDS24955.1. [Q9JM52-1]
RefSeqiNP_057922.2. NM_016713.2. [Q9JM52-2]
NP_795712.2. NM_176893.2. [Q9JM52-1]
XP_006533744.1. XM_006533681.1. [Q9JM52-3]
XP_006537239.1. XM_006537176.1. [Q9JM52-3]
UniGeneiMm.42967.

Genome annotation databases

EnsembliENSMUST00000102558; ENSMUSP00000099618; ENSMUSG00000020827. [Q9JM52-2]
ENSMUST00000102559; ENSMUSP00000099619; ENSMUSG00000020827. [Q9JM52-1]
GeneIDi50932.
KEGGimmu:50932.
UCSCiuc007jvj.1. mouse. [Q9JM52-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB041925 mRNA. Translation: BAA94837.1 .
AB035697 mRNA. Translation: BAA90752.1 .
AL592547 Genomic DNA. Translation: CAI24010.1 .
CR933736 Genomic DNA. Translation: CAM28122.1 .
CH466596 Genomic DNA. Translation: EDL12579.1 .
BC011346 mRNA. Translation: AAH11346.1 .
CCDSi CCDS24954.1. [Q9JM52-2 ]
CCDS24955.1. [Q9JM52-1 ]
RefSeqi NP_057922.2. NM_016713.2. [Q9JM52-2 ]
NP_795712.2. NM_176893.2. [Q9JM52-1 ]
XP_006533744.1. XM_006533681.1. [Q9JM52-3 ]
XP_006537239.1. XM_006537176.1. [Q9JM52-3 ]
UniGenei Mm.42967.

3D structure databases

ProteinModelPortali Q9JM52.
SMRi Q9JM52. Positions 12-311.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9JM52. 4 interactions.
MINTi MINT-1616244.

PTM databases

PhosphoSitei Q9JM52.

Proteomic databases

MaxQBi Q9JM52.
PaxDbi Q9JM52.
PRIDEi Q9JM52.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000102558 ; ENSMUSP00000099618 ; ENSMUSG00000020827 . [Q9JM52-2 ]
ENSMUST00000102559 ; ENSMUSP00000099619 ; ENSMUSG00000020827 . [Q9JM52-1 ]
GeneIDi 50932.
KEGGi mmu:50932.
UCSCi uc007jvj.1. mouse. [Q9JM52-1 ]

Organism-specific databases

CTDi 50488.
MGIi MGI:1355329. Mink1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000118848.
HOGENOMi HOG000290708.
HOVERGENi HBG036506.
InParanoidi Q9JM52.
KOi K04413.

Enzyme and pathway databases

Reactomei REACT_188970. Oxidative Stress Induced Senescence.

Miscellaneous databases

NextBioi 307959.
PROi Q9JM52.
SOURCEi Search...

Gene expression databases

Bgeei Q9JM52.
CleanExi MM_MINK1.
ExpressionAtlasi Q9JM52. baseline and differential.
Genevestigatori Q9JM52.

Family and domain databases

InterProi IPR001180. Citron.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00780. CNH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00036. CNH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50219. CNH. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of MINK, a novel member of mammalian GCK family kinases, which is up-regulated during postnatal mouse cerebral development."
    Dan I., Watanabe N.M., Kobayashi T., Yamashita-Suzuki K., Fukagaya Y., Kajikawa E., Kimura W.K., Nakashima T.M., Matsumoto K., Ninomiya-Tsuji J., Kusumi A.
    FEBS Lett. 469:19-23(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: ICR.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 148-1308 (ISOFORM 3).
    Tissue: Mammary gland.
  5. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 735-742 AND 908-920, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  6. "Signaling by the kinase MINK is essential in the negative selection of autoreactive thymocytes."
    McCarty N., Paust S., Ikizawa K., Dan I., Li X., Cantor H.
    Nat. Immunol. 6:65-72(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NCK1.
  7. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  8. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-729, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMINK1_MOUSE
AccessioniPrimary (citable) accession number: Q9JM52
Secondary accession number(s): Q5SXG2, Q921M6, Q9JM92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3