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Protein

Calsenilin

Gene

Kcnip3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception (By similarity).By similarity
Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner (By similarity).By similarity
May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of beta-amyloid formation (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi175 – 186121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi223 – 234122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Repressor, Voltage-gated channel

Keywords - Biological processi

Apoptosis, Ion transport, Potassium transport, Transcription, Transcription regulation, Transport

Keywords - Ligandi

Calcium, Metal-binding, Potassium

Enzyme and pathway databases

ReactomeiR-RNO-5576894. Phase 1 - inactivation of fast Na+ channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Calsenilin
Alternative name(s):
A-type potassium channel modulatory protein 3
DRE-antagonist modulator
Short name:
DREAM
Kv channel-interacting protein 3
Short name:
KChIP3
Gene namesi
Name:Kcnip3
Synonyms:Csen, Dream, Kchip3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi70888. Kcnip3.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cell membrane 2 Publications; Lipid-anchor 1 Publication
  • Endoplasmic reticulum 1 Publication
  • Golgi apparatus 1 Publication
  • Nucleus By similarity

  • Note: Also membrane-bound, associated with the plasma membrane. In the presence of PSEN2 associated with the endoplasmic reticulum and Golgi. The sumoylated form is present only in the nucleus.By similarity

GO - Cellular componenti

  • axon Source: RGD
  • axon terminus Source: RGD
  • cytosol Source: UniProtKB
  • dendrite Source: RGD
  • endoplasmic reticulum Source: UniProtKB-SubCell
  • Golgi apparatus Source: UniProtKB-SubCell
  • nucleus Source: RGD
  • protein-DNA complex Source: RGD
  • voltage-gated potassium channel complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi45 – 462CC → AA or SS: Greatly reduces plasma membrane localization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 256256CalsenilinPRO_0000073817Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphoserineCombined sources
Cross-linki26 – 26Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Lipidationi45 – 451S-palmitoyl cysteine1 Publication
Lipidationi46 – 461S-palmitoyl cysteine1 Publication
Modified residuei60 – 601PhosphoserineBy similarity
Modified residuei63 – 631PhosphoserineBy similarity
Cross-linki90 – 90Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity

Post-translational modificationi

Palmitoylated. Palmitoylation enhances association with the plasma membrane.1 Publication
Proteolytically cleaved by caspase-3.By similarity

Keywords - PTMi

Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9JM47.

PTM databases

iPTMnetiQ9JM47.
SwissPalmiQ9JM47.

Expressioni

Tissue specificityi

Detected in brain cortex, thalamus, dentate gyrus and cerebellum (at protein level) (PubMed:16123112). Expressed in brain. Colocalizes with KCND2 in excitatory neurons including cortical and hippocampal CA1 pyramidal cells.2 Publications

Gene expression databases

GenevisibleiQ9JM47. RN.

Interactioni

Subunit structurei

Binds to DNA as a homomultimer. Dimerization is induced by binding to calcium. Interacts with the C-terminus of PSEN1 and PSEN2 and with PSEN2 CTF subunit. Associates with KCN1. Component of heteromultimeric potassium channels (PubMed:16123112). Identified in potassium channel complexes containing KCND1, KCND2, KCND3, KCNIP1, KCNIP2, KCNIP3, KCNIP4, DPP6 and DPP10 (By similarity). Interacts with KCND2 and KCND3 (PubMed:10676964).By similarity4 Publications

GO - Molecular functioni

  • ion channel binding Source: RGD

Protein-protein interaction databases

BioGridi249311. 1 interaction.
STRINGi10116.ENSRNOP00000047927.

Structurei

3D structure databases

ProteinModelPortaliQ9JM47.
SMRiQ9JM47. Positions 76-256.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini67 – 12357EF-hand 1; degeneratePROSITE-ProRule annotationAdd
BLAST
Domaini126 – 16136EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini162 – 19736EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini210 – 24536EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni243 – 25614Interaction with KCND2By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the recoverin family.Curated
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiQ9JM47.
OMAiFGILEPM.
OrthoDBiEOG7GJ6F3.
PhylomeDBiQ9JM47.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JM47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRTKEAMKA SDGSLLGDPG RIPLSKREGI KWQRPRFTRQ ALMRCCLIKW
60 70 80 90 100
ILSSAAPQGS DSSDSELELS TVRHQPEGLD QLQAQTKFTK KELQSLYRGF
110 120 130 140 150
KNECPTGLVD EDTFKLIYSQ FFPQGDATTY AHFLFNAFDA DGNGAIHFED
160 170 180 190 200
FVVGLSILLR GTVHEKLKWA FNLYDINKDG YITKEEMLAI MKSIYDMMGR
210 220 230 240 250
HTYPILREDA PLEHVERFFQ KMDRNQDGVV TIDEFLETCQ KDENIMSSMQ

LFENVI
Length:256
Mass (Da):29,471
Last modified:October 1, 2000 - v1
Checksum:i6DF71E1584C1E9A0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti208 – 2081E → K in AAG15382 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB043892 mRNA. Translation: BAA96360.1.
AF297118 mRNA. Translation: AAG15382.1.
RefSeqiNP_115851.1. NM_032462.2.
UniGeneiRn.74239.

Genome annotation databases

EnsembliENSRNOT00000078994; ENSRNOP00000071572; ENSRNOG00000014152.
GeneIDi65199.
KEGGirno:65199.
UCSCiRGD:70888. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB043892 mRNA. Translation: BAA96360.1.
AF297118 mRNA. Translation: AAG15382.1.
RefSeqiNP_115851.1. NM_032462.2.
UniGeneiRn.74239.

3D structure databases

ProteinModelPortaliQ9JM47.
SMRiQ9JM47. Positions 76-256.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249311. 1 interaction.
STRINGi10116.ENSRNOP00000047927.

PTM databases

iPTMnetiQ9JM47.
SwissPalmiQ9JM47.

Proteomic databases

PaxDbiQ9JM47.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000078994; ENSRNOP00000071572; ENSRNOG00000014152.
GeneIDi65199.
KEGGirno:65199.
UCSCiRGD:70888. rat.

Organism-specific databases

CTDi30818.
RGDi70888. Kcnip3.

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiQ9JM47.
OMAiFGILEPM.
OrthoDBiEOG7GJ6F3.
PhylomeDBiQ9JM47.

Enzyme and pathway databases

ReactomeiR-RNO-5576894. Phase 1 - inactivation of fast Na+ channels.

Miscellaneous databases

PROiQ9JM47.

Gene expression databases

GenevisibleiQ9JM47. RN.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A-type potassium channel modulating protein 3."
    Ohya S., Imaizumi Y.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Molecular cloning of the rat DREAM gene."
    Leclerc G.M., Shorte S.L., Leclerc G.J., Frawley L.S.
    Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Pituitary.
  3. Cited for: INTERACTION WITH KCND2 AND KCND3.
  4. "Palmitoylation of KChIP splicing variants is required for efficient cell surface expression of Kv4.3 channels."
    Takimoto K., Yang E.-K., Conforti L.
    J. Biol. Chem. 277:26904-26911(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, PALMITOYLATION AT CYS-45 AND CYS-46, MUTAGENESIS OF 45-CYS-CYS-46.
  5. "KChIP3 rescues the functional expression of Shal channel tetramerization mutants."
    Kunjilwar K., Strang C., DeRubeis D., Pfaffinger P.J.
    J. Biol. Chem. 279:54542-54551(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KCND2.
  6. "KChIPs and Kv4 alpha subunits as integral components of A-type potassium channels in mammalian brain."
    Rhodes K.J., Carroll K.I., Sung M.A., Doliveira L.C., Monaghan M.M., Burke S.L., Strassle B.W., Buchwalder L., Menegola M., Cao J., An W.F., Trimmer J.S.
    J. Neurosci. 24:7903-7915(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INTERACTION WITH KCND2.
  7. "Multiprotein assembly of Kv4.2, KChIP3 and DPP10 produces ternary channel complexes with ISA-like properties."
    Jerng H.H., Kunjilwar K., Pfaffinger P.J.
    J. Physiol. (Lond.) 568:767-788(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KCND2 AND DPP10, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCSEN_RAT
AccessioniPrimary (citable) accession number: Q9JM47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 1, 2000
Last modified: July 6, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.