Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sodium-coupled neutral amino acid transporter 1

Gene

Slc38a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters glutamate and GABA.4 Publications

Enzyme regulationi

Inhibited by lithium, potassium, choline ions, N-methyl-D-glucamine and 2-methylamino-isobutyric acid (MeAIB).2 Publications

Kineticsi

  1. KM=489 µM for L-glutamine (at pH 7.4)1 Publication
  2. KM=582 µM for L-glutamine (at pH 8.2)1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Biological processi

    Amino-acid transport, Ion transport, Sodium transport, Symport, Transport

    Keywords - Ligandi

    Sodium

    Enzyme and pathway databases

    ReactomeiR-RNO-210455. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
    R-RNO-352230. Amino acid transport across the plasma membrane.

    Protein family/group databases

    TCDBi2.A.18.6.1. the amino acid/auxin permease (aaap) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium-coupled neutral amino acid transporter 1
    Alternative name(s):
    Amino acid transporter A1
    Short name:
    rATA1
    Glutamine transporter
    N-system amino acid transporter 2
    Solute carrier family 38 member 1
    System A amino acid transporter 1
    System A transporter 2
    System N amino acid transporter 1
    Gene namesi
    Name:Slc38a1
    Synonyms:Ata1, Glnt, Sa2, Sat1, Snat1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 7

    Organism-specific databases

    RGDi69645. Slc38a1.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 7474CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei75 – 9723HelicalSequence analysisAdd
    BLAST
    Topological domaini98 – 11215ExtracellularSequence analysisAdd
    BLAST
    Transmembranei113 – 13321HelicalSequence analysisAdd
    BLAST
    Topological domaini134 – 14815CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei149 – 16921HelicalSequence analysisAdd
    BLAST
    Topological domaini170 – 18819ExtracellularSequence analysisAdd
    BLAST
    Transmembranei189 – 21123HelicalSequence analysisAdd
    BLAST
    Topological domaini212 – 2165CytoplasmicSequence analysis
    Transmembranei217 – 23721HelicalSequence analysisAdd
    BLAST
    Topological domaini238 – 27336ExtracellularSequence analysisAdd
    BLAST
    Transmembranei274 – 29421HelicalSequence analysisAdd
    BLAST
    Topological domaini295 – 31016CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei311 – 33121HelicalSequence analysisAdd
    BLAST
    Topological domaini332 – 34817ExtracellularSequence analysisAdd
    BLAST
    Transmembranei349 – 36921HelicalSequence analysisAdd
    BLAST
    Topological domaini370 – 39122CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei392 – 41221HelicalSequence analysisAdd
    BLAST
    Topological domaini413 – 4142ExtracellularSequence analysis
    Transmembranei415 – 43521HelicalSequence analysisAdd
    BLAST
    Topological domaini436 – 45015CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei451 – 47121HelicalSequence analysisAdd
    BLAST
    Topological domaini472 – 48514ExtracellularSequence analysisAdd
    BLAST

    GO - Cellular componenti

    • axon Source: Ensembl
    • integral component of plasma membrane Source: GO_Central
    • plasma membrane Source: RGD
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 485485Sodium-coupled neutral amino acid transporter 1PRO_0000310478Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei6 – 61PhosphoserineBy similarity
    Modified residuei11 – 111PhosphothreonineBy similarity
    Modified residuei25 – 251PhosphoserineBy similarity
    Modified residuei28 – 281PhosphoserineBy similarity
    Modified residuei49 – 491PhosphoserineBy similarity
    Modified residuei52 – 521PhosphoserineCombined sources
    Modified residuei54 – 541PhosphothreonineCombined sources
    Modified residuei56 – 561PhosphoserineCombined sources
    Glycosylationi251 – 2511N-linked (GlcNAc...)Sequence analysis
    Glycosylationi257 – 2571N-linked (GlcNAc...)Sequence analysis

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ9JM15.
    PRIDEiQ9JM15.

    PTM databases

    iPTMnetiQ9JM15.

    Expressioni

    Tissue specificityi

    Specifically expressed in brain with the highest levels in cerebellum and thalamus (at protein level). Expressed in glutamatergic, GABAergic and a subset of dopaminergic neurons of the substantia nigra and cholinergic motoneurons (at protein level). Also expressed by ependymal cells lining the ventricle (at protein level). Expression is also detected in spinal cord, heart, colon and placenta.6 Publications

    Developmental stagei

    Expressed at all stages examined including E17, E19, P2, P10 and P14. Expressed in neocortex, hippocampus and neuroepithelium at E17 and more prominently expressed in striatum, hippocampus and cortex at postnatal days (at protein level).1 Publication

    Inductioni

    Up-regulated by forskolin in astrocytes.1 Publication

    Gene expression databases

    GenevisibleiQ9JM15. RN.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000008138.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1305. Eukaryota.
    COG0814. LUCA.
    GeneTreeiENSGT00760000119147.
    HOGENOMiHOG000013088.
    HOVERGENiHBG059571.
    InParanoidiQ9JM15.
    KOiK14990.
    OMAiHTVVTCI.
    OrthoDBiEOG7WHH9J.
    PhylomeDBiQ9JM15.
    TreeFamiTF328787.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9JM15-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MMHFKSGLEL TELQNMTVPE DDNVSNDSND FTEVENGQIN SKFISDRESR
    60 70 80 90 100
    RSLTNSHLEK RKCDEYIPGT TSLGMSVFNL SNAIMGSGIL GLAFALANTG
    110 120 130 140 150
    ILLFLILLTS VTLLSIYSIN LLLICSKETG CMVYEKLGEQ VFGTTGKLVI
    160 170 180 190 200
    FGATSLQNTG AMLSYLFIVK NELPSAIKSL MGEEETFSAW YVDGRVLVVM
    210 220 230 240 250
    VTFGIILPLC LLKNLGYLGY TSGFSLSCMV FFLIVVIYKK FQIPCMNGEQ
    260 270 280 290 300
    NSTVSANVTD ACTPKYVTFN SKTVYALPTI AFAFVCHPSV LPIYSELKDR
    310 320 330 340 350
    SQKKMQMVSN ISFFAMFVMY FLTAIFGYLT FYEKVQSDLL HKYQSTGDIL
    360 370 380 390 400
    ILTVRLAVIV AVILTVPVLF FTVRSSLFEL AKKTKFHLCR HVLVTIILLV
    410 420 430 440 450
    IINLLVIFIP SMKDIFGVVG VTSANMLIFI LPSSLYLKIT NQDGDKNTQR
    460 470 480
    IWAALFLALG VLFSLISIPL VIYDWACSSS NGEGH
    Length:485
    Mass (Da):53,846
    Last modified:October 1, 2000 - v1
    Checksum:i0CDAD864513FC186
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF075704 mRNA. Translation: AAF34240.1.
    BC097283 mRNA. Translation: AAH97283.1.
    RefSeqiNP_620187.1. NM_138832.1.
    XP_006242339.1. XM_006242277.2.
    XP_006242340.1. XM_006242278.2.
    XP_006242341.1. XM_006242279.2.
    XP_006242342.1. XM_006242280.2.
    XP_006242343.1. XM_006242281.2.
    UniGeneiRn.162022.
    Rn.96943.

    Genome annotation databases

    EnsembliENSRNOT00000008138; ENSRNOP00000008138; ENSRNOG00000005291.
    ENSRNOT00000089806; ENSRNOP00000070624; ENSRNOG00000005291.
    GeneIDi170567.
    KEGGirno:170567.
    UCSCiRGD:69645. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF075704 mRNA. Translation: AAF34240.1.
    BC097283 mRNA. Translation: AAH97283.1.
    RefSeqiNP_620187.1. NM_138832.1.
    XP_006242339.1. XM_006242277.2.
    XP_006242340.1. XM_006242278.2.
    XP_006242341.1. XM_006242279.2.
    XP_006242342.1. XM_006242280.2.
    XP_006242343.1. XM_006242281.2.
    UniGeneiRn.162022.
    Rn.96943.

    3D structure databases

    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000008138.

    Protein family/group databases

    TCDBi2.A.18.6.1. the amino acid/auxin permease (aaap) family.

    PTM databases

    iPTMnetiQ9JM15.

    Proteomic databases

    PaxDbiQ9JM15.
    PRIDEiQ9JM15.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSRNOT00000008138; ENSRNOP00000008138; ENSRNOG00000005291.
    ENSRNOT00000089806; ENSRNOP00000070624; ENSRNOG00000005291.
    GeneIDi170567.
    KEGGirno:170567.
    UCSCiRGD:69645. rat.

    Organism-specific databases

    CTDi81539.
    RGDi69645. Slc38a1.

    Phylogenomic databases

    eggNOGiKOG1305. Eukaryota.
    COG0814. LUCA.
    GeneTreeiENSGT00760000119147.
    HOGENOMiHOG000013088.
    HOVERGENiHBG059571.
    InParanoidiQ9JM15.
    KOiK14990.
    OMAiHTVVTCI.
    OrthoDBiEOG7WHH9J.
    PhylomeDBiQ9JM15.
    TreeFamiTF328787.

    Enzyme and pathway databases

    ReactomeiR-RNO-210455. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
    R-RNO-352230. Amino acid transport across the plasma membrane.

    Miscellaneous databases

    PROiQ9JM15.

    Gene expression databases

    GenevisibleiQ9JM15. RN.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Cloning and functional identification of a neuronal glutamine transporter."
      Varoqui H., Zhu H., Yao D., Ming H., Erickson J.D.
      J. Biol. Chem. 275:4049-4054(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Neuron.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta.
    3. Cited for: FUNCTION, ENZYME REGULATION.
    4. "Glutamine uptake by neurons: interaction of protons with system a transporters."
      Chaudhry F.A., Schmitz D., Reimer R.J., Larsson P., Gray A.T., Nicoll R., Kavanaugh M., Edwards R.H.
      J. Neurosci. 22:62-72(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    5. Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    6. "Functional properties and cellular distribution of the system A glutamine transporter SNAT1 support specialized roles in central neurons."
      Mackenzie B., Schaefer M.K.-H., Erickson J.D., Hediger M.A., Weihe E., Varoqui H.
      J. Biol. Chem. 278:23720-23730(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    7. "Localization of the glutamine transporter SNAT1 in rat cerebral cortex and neighboring structures, with a note on its localization in human cortex."
      Melone M., Quagliano F., Barbaresi P., Varoqui H., Erickson J.D., Conti F.
      Cereb. Cortex 14:562-574(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    8. Cited for: TISSUE SPECIFICITY.
    9. "Upregulation of the glutamine transporter through transactivation mediated by cAMP/protein kinase A signals toward exacerbation of vulnerability to oxidative stress in rat neocortical astrocytes."
      Ogura M., Taniura H., Nakamichi N., Yoneda Y.
      J. Cell. Physiol. 212:375-385(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY FORSKOLIN.
    10. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
      Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
      Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52; THR-54 AND SER-56, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiS38A1_RAT
    AccessioniPrimary (citable) accession number: Q9JM15
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: October 1, 2000
    Last modified: July 6, 2016
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.