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Protein

Sodium-coupled neutral amino acid transporter 1

Gene

Slc38a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters glutamate and GABA.4 Publications

Enzyme regulationi

Inhibited by lithium, potassium, choline ions, N-methyl-D-glucamine and 2-methylamino-isobutyric acid (MeAIB).2 Publications

Kineticsi

  1. KM=489 µM for L-glutamine (at pH 7.4)1 Publication
  2. KM=582 µM for L-glutamine (at pH 8.2)1 Publication

    GO - Molecular functioni

    GO - Biological processi

    • amino acid transmembrane transport Source: GO_Central
    • female pregnancy Source: RGD
    • sodium ion transport Source: UniProtKB-KW

    Keywordsi

    Biological processAmino-acid transport, Ion transport, Sodium transport, Symport, Transport
    LigandSodium

    Enzyme and pathway databases

    ReactomeiR-RNO-210455 Astrocytic Glutamate-Glutamine Uptake And Metabolism
    R-RNO-352230 Amino acid transport across the plasma membrane

    Protein family/group databases

    TCDBi2.A.18.6.1 the amino acid/auxin permease (aaap) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium-coupled neutral amino acid transporter 1
    Alternative name(s):
    Amino acid transporter A1
    Short name:
    rATA1
    Glutamine transporter
    N-system amino acid transporter 2
    Solute carrier family 38 member 1
    System A amino acid transporter 1
    System A transporter 2
    System N amino acid transporter 1
    Gene namesi
    Name:Slc38a1
    Synonyms:Ata1, Glnt, Sa2, Sat1, Snat1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 7

    Organism-specific databases

    RGDi69645 Slc38a1

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 74CytoplasmicSequence analysisAdd BLAST74
    Transmembranei75 – 97HelicalSequence analysisAdd BLAST23
    Topological domaini98 – 112ExtracellularSequence analysisAdd BLAST15
    Transmembranei113 – 133HelicalSequence analysisAdd BLAST21
    Topological domaini134 – 148CytoplasmicSequence analysisAdd BLAST15
    Transmembranei149 – 169HelicalSequence analysisAdd BLAST21
    Topological domaini170 – 188ExtracellularSequence analysisAdd BLAST19
    Transmembranei189 – 211HelicalSequence analysisAdd BLAST23
    Topological domaini212 – 216CytoplasmicSequence analysis5
    Transmembranei217 – 237HelicalSequence analysisAdd BLAST21
    Topological domaini238 – 273ExtracellularSequence analysisAdd BLAST36
    Transmembranei274 – 294HelicalSequence analysisAdd BLAST21
    Topological domaini295 – 310CytoplasmicSequence analysisAdd BLAST16
    Transmembranei311 – 331HelicalSequence analysisAdd BLAST21
    Topological domaini332 – 348ExtracellularSequence analysisAdd BLAST17
    Transmembranei349 – 369HelicalSequence analysisAdd BLAST21
    Topological domaini370 – 391CytoplasmicSequence analysisAdd BLAST22
    Transmembranei392 – 412HelicalSequence analysisAdd BLAST21
    Topological domaini413 – 414ExtracellularSequence analysis2
    Transmembranei415 – 435HelicalSequence analysisAdd BLAST21
    Topological domaini436 – 450CytoplasmicSequence analysisAdd BLAST15
    Transmembranei451 – 471HelicalSequence analysisAdd BLAST21
    Topological domaini472 – 485ExtracellularSequence analysisAdd BLAST14

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003104781 – 485Sodium-coupled neutral amino acid transporter 1Add BLAST485

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei6PhosphoserineBy similarity1
    Modified residuei11PhosphothreonineBy similarity1
    Modified residuei25PhosphoserineBy similarity1
    Modified residuei28PhosphoserineBy similarity1
    Modified residuei49PhosphoserineBy similarity1
    Modified residuei52PhosphoserineCombined sources1
    Modified residuei54PhosphothreonineCombined sources1
    Modified residuei56PhosphoserineCombined sources1
    Disulfide bondi245 ↔ 262PROSITE-ProRule annotation
    Glycosylationi251N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi257N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ9JM15
    PRIDEiQ9JM15

    PTM databases

    iPTMnetiQ9JM15
    PhosphoSitePlusiQ9JM15

    Expressioni

    Tissue specificityi

    Specifically expressed in brain with the highest levels in cerebellum and thalamus (at protein level). Expressed in glutamatergic, GABAergic and a subset of dopaminergic neurons of the substantia nigra and cholinergic motoneurons (at protein level). Also expressed by ependymal cells lining the ventricle (at protein level). Expression is also detected in spinal cord, heart, colon and placenta.6 Publications

    Developmental stagei

    Expressed at all stages examined including E17, E19, P2, P10 and P14. Expressed in neocortex, hippocampus and neuroepithelium at E17 and more prominently expressed in striatum, hippocampus and cortex at postnatal days (at protein level).1 Publication

    Inductioni

    Up-regulated by forskolin in astrocytes.1 Publication

    Gene expression databases

    BgeeiENSRNOG00000005291
    GenevisibleiQ9JM15 RN

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000008138

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1305 Eukaryota
    COG0814 LUCA
    GeneTreeiENSGT00760000119147
    HOGENOMiHOG000013088
    HOVERGENiHBG059571
    InParanoidiQ9JM15
    KOiK14990
    OMAiPLVIYDW
    PhylomeDBiQ9JM15
    TreeFamiTF328787

    Family and domain databases

    InterProiView protein in InterPro
    IPR013057 AA_transpt_TM
    PfamiView protein in Pfam
    PF01490 Aa_trans, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9JM15-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MMHFKSGLEL TELQNMTVPE DDNVSNDSND FTEVENGQIN SKFISDRESR
    60 70 80 90 100
    RSLTNSHLEK RKCDEYIPGT TSLGMSVFNL SNAIMGSGIL GLAFALANTG
    110 120 130 140 150
    ILLFLILLTS VTLLSIYSIN LLLICSKETG CMVYEKLGEQ VFGTTGKLVI
    160 170 180 190 200
    FGATSLQNTG AMLSYLFIVK NELPSAIKSL MGEEETFSAW YVDGRVLVVM
    210 220 230 240 250
    VTFGIILPLC LLKNLGYLGY TSGFSLSCMV FFLIVVIYKK FQIPCMNGEQ
    260 270 280 290 300
    NSTVSANVTD ACTPKYVTFN SKTVYALPTI AFAFVCHPSV LPIYSELKDR
    310 320 330 340 350
    SQKKMQMVSN ISFFAMFVMY FLTAIFGYLT FYEKVQSDLL HKYQSTGDIL
    360 370 380 390 400
    ILTVRLAVIV AVILTVPVLF FTVRSSLFEL AKKTKFHLCR HVLVTIILLV
    410 420 430 440 450
    IINLLVIFIP SMKDIFGVVG VTSANMLIFI LPSSLYLKIT NQDGDKNTQR
    460 470 480
    IWAALFLALG VLFSLISIPL VIYDWACSSS NGEGH
    Length:485
    Mass (Da):53,846
    Last modified:October 1, 2000 - v1
    Checksum:i0CDAD864513FC186
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF075704 mRNA Translation: AAF34240.1
    BC097283 mRNA Translation: AAH97283.1
    RefSeqiNP_620187.1, NM_138832.1
    XP_006242339.1, XM_006242277.3
    XP_006242340.1, XM_006242278.3
    XP_006242341.1, XM_006242279.3
    XP_006242342.1, XM_006242280.3
    XP_006242343.1, XM_006242281.3
    XP_017450126.1, XM_017594637.1
    UniGeneiRn.162022
    Rn.96943

    Genome annotation databases

    EnsembliENSRNOT00000008138; ENSRNOP00000008138; ENSRNOG00000005291
    ENSRNOT00000089806; ENSRNOP00000070624; ENSRNOG00000005291
    GeneIDi170567
    KEGGirno:170567
    UCSCiRGD:69645 rat

    Similar proteinsi

    Entry informationi

    Entry nameiS38A1_RAT
    AccessioniPrimary (citable) accession number: Q9JM15
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: October 1, 2000
    Last modified: May 23, 2018
    This is version 107 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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