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Protein

5'(3')-deoxyribonucleotidase, cytosolic type

Gene

Nt5c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides, with a preference for dUMP and dTMP, intermediate activity towards dGMP, and low activity towards dCMP and dAMP.1 Publication

Catalytic activityi

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Mg2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei12NucleophileCurated1
Metal bindingi12Magnesium1
Active sitei14Proton donorCurated1
Metal bindingi14Magnesium; via carbonyl oxygen1
Binding sitei20Substrate1
Binding sitei46Substrate1
Binding sitei67Substrate1
Binding sitei101Substrate1
Binding sitei136Substrate1
Metal bindingi147Magnesium1

GO - Molecular functioni

  • 5'-nucleotidase activity Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • nucleotidase activity Source: MGI
  • phosphatase activity Source: MGI
  • pyrimidine nucleotide binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.1.3.34. 3474.
ReactomeiR-MMU-73621. Pyrimidine catabolism.
R-MMU-74259. Purine catabolism.
SABIO-RKQ9JM14.

Names & Taxonomyi

Protein namesi
Recommended name:
5'(3')-deoxyribonucleotidase, cytosolic type (EC:3.1.3.-)
Alternative name(s):
Cytosolic 5',3'-pyrimidine nucleotidase
Deoxy-5'-nucleotidase 1
Short name:
dNT-1
Gene namesi
Name:Nt5c
Synonyms:Dnt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1354954. Nt5c.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • mitochondrion Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001643721 – 2005'(3')-deoxyribonucleotidase, cytosolic typeAdd BLAST200

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei102PhosphothreonineCombined sources1
Modified residuei184PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9JM14.
MaxQBiQ9JM14.
PaxDbiQ9JM14.
PeptideAtlasiQ9JM14.
PRIDEiQ9JM14.

2D gel databases

REPRODUCTION-2DPAGEIPI00124639.

PTM databases

iPTMnetiQ9JM14.
PhosphoSitePlusiQ9JM14.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020736.
CleanExiMM_NT5C.
ExpressionAtlasiQ9JM14. baseline and differential.
GenevisibleiQ9JM14. MM.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiQ9JM14. 2 interactors.
MINTiMINT-1845792.
STRINGi10090.ENSMUSP00000021082.

Structurei

Secondary structure

1200
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 14Combined sources8
Turni15 – 17Combined sources3
Helixi20 – 31Combined sources12
Helixi40 – 42Combined sources3
Helixi48 – 55Combined sources8
Helixi59 – 67Combined sources9
Turni70 – 75Combined sources6
Helixi82 – 90Combined sources9
Beta strandi95 – 101Combined sources7
Turni108 – 110Combined sources3
Helixi111 – 121Combined sources11
Helixi124 – 127Combined sources4
Beta strandi130 – 132Combined sources3
Helixi136 – 138Combined sources3
Beta strandi142 – 147Combined sources6
Beta strandi159 – 165Combined sources7
Helixi168 – 170Combined sources3
Beta strandi179 – 182Combined sources4
Helixi189 – 197Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JAOX-ray2.00A1-200[»]
2JARX-ray1.94A1-200[»]
ProteinModelPortaliQ9JM14.
SMRiQ9JM14.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JM14.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IHG0. Eukaryota.
ENOG410XQYS. LUCA.
GeneTreeiENSGT00390000011596.
HOGENOMiHOG000236944.
HOVERGENiHBG045599.
InParanoidiQ9JM14.
KOiK01081.
OMAiFTCCHNR.
OrthoDBiEOG091G0L73.
PhylomeDBiQ9JM14.
TreeFamiTF331117.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR010708. 5'(3')-deoxyribonucleotidase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF06941. NT5C. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9JM14-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVKRPVRVL VDMDGVLADF ESGLLQGFRR RFPEEPHVPL EQRRGFLANE
60 70 80 90 100
QYGALRPDLA EKVASVYESP GFFLNLEPIP GALDALREMN DMKDTEVFIC
110 120 130 140 150
TTPLLKYDHC VGEKYRWVEQ NLGPEFVERI ILTRDKTVVM GDLLIDDKDN
160 170 180 190 200
IQGLEETPSW EHILFTCCHN QHLALPPTRR RLLSWSDNWR GIIESKRASL
Length:200
Mass (Da):23,076
Last modified:October 1, 2000 - v1
Checksum:i5E5A3AFB0A214082
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF078840 mRNA. Translation: AAF36421.1.
AK007418 mRNA. Translation: BAB25027.1.
BC024368 mRNA. Translation: AAH24368.1.
BK000208 Genomic DNA. Translation: DAA00069.1.
CCDSiCCDS25637.1.
RefSeqiNP_056622.1. NM_015807.1.
UniGeneiMm.390379.

Genome annotation databases

EnsembliENSMUST00000021082; ENSMUSP00000021082; ENSMUSG00000020736.
GeneIDi50773.
KEGGimmu:50773.
UCSCiuc007mhu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF078840 mRNA. Translation: AAF36421.1.
AK007418 mRNA. Translation: BAB25027.1.
BC024368 mRNA. Translation: AAH24368.1.
BK000208 Genomic DNA. Translation: DAA00069.1.
CCDSiCCDS25637.1.
RefSeqiNP_056622.1. NM_015807.1.
UniGeneiMm.390379.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JAOX-ray2.00A1-200[»]
2JARX-ray1.94A1-200[»]
ProteinModelPortaliQ9JM14.
SMRiQ9JM14.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JM14. 2 interactors.
MINTiMINT-1845792.
STRINGi10090.ENSMUSP00000021082.

PTM databases

iPTMnetiQ9JM14.
PhosphoSitePlusiQ9JM14.

2D gel databases

REPRODUCTION-2DPAGEIPI00124639.

Proteomic databases

EPDiQ9JM14.
MaxQBiQ9JM14.
PaxDbiQ9JM14.
PeptideAtlasiQ9JM14.
PRIDEiQ9JM14.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021082; ENSMUSP00000021082; ENSMUSG00000020736.
GeneIDi50773.
KEGGimmu:50773.
UCSCiuc007mhu.1. mouse.

Organism-specific databases

CTDi30833.
MGIiMGI:1354954. Nt5c.

Phylogenomic databases

eggNOGiENOG410IHG0. Eukaryota.
ENOG410XQYS. LUCA.
GeneTreeiENSGT00390000011596.
HOGENOMiHOG000236944.
HOVERGENiHBG045599.
InParanoidiQ9JM14.
KOiK01081.
OMAiFTCCHNR.
OrthoDBiEOG091G0L73.
PhylomeDBiQ9JM14.
TreeFamiTF331117.

Enzyme and pathway databases

BRENDAi3.1.3.34. 3474.
ReactomeiR-MMU-73621. Pyrimidine catabolism.
R-MMU-74259. Purine catabolism.
SABIO-RKQ9JM14.

Miscellaneous databases

EvolutionaryTraceiQ9JM14.
PROiQ9JM14.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020736.
CleanExiMM_NT5C.
ExpressionAtlasiQ9JM14. baseline and differential.
GenevisibleiQ9JM14. MM.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR010708. 5'(3')-deoxyribonucleotidase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF06941. NT5C. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNT5C_MOUSE
AccessioniPrimary (citable) accession number: Q9JM14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.