Q9JM01 (CDKL3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase-like 3
EC=2.7.11.22

Alternative name(s):
Serine/threonine protein kinase NKIATRE

Gene names

Name:	Cdkl3
Synonyms:	Nkiatre

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Isoform 2: Nucleus. Cytoplasm Ref.1. Isoform 3: Cytoplasm Ref.1.
Tissue specificity	Highly expressed in brain, and to a lower extent in heart and testis. Ref.1
Domain	The [NKR]KIAxRE motif seems to be a cyclin-binding region.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9JM01-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9JM01-2)

Also known as: NKIATRE alpha; p57 alpha isoform;

The sequence of this isoform differs from the canonical sequence as follows:
     504-505: DQ → AK
     506-593: Missing.

Note: Found in the nucleus and cytoplasm.

Isoform 3 (identifier: Q9JM01-3)

Also known as: NKIATRE beta; p52 beta isoform;

The sequence of this isoform differs from the canonical sequence as follows:
     458-593: Missing.

Note: Found in the cytoplasm.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 593	593	Cyclin-dependent kinase-like 3		PRO_0000085823
Regions
	Domain	4 – 286	283	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
	Motif	45 – 51	7	[NKR]KIAxRE
Sites
	Active site	125	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
Natural variations
	Alternative sequence	458 – 593	136	Missing in isoform 3.		VSP_016158
	Alternative sequence	504 – 505	2	DQ → AK in isoform 2.		VSP_016159
	Alternative sequence	506 – 593	88	Missing in isoform 2.		VSP_016160

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 22, 2005. Version 2. Checksum: 40F8C846EA421E0B	FASTA	593	67,553
	10 20 30 40 50 60 MEMYETLGKV GEGSYGTVMK CKHKDTGRIV AIKIFYEKPE KSVNKIATRE IKFLKQFRHE 70 80 90 100 110 120 NLVNLIEVFR QKKKIHLVFE FIDHTVLDEL QHYCHGLESK RLRKYLFQIL RAIEYLHNNN 130 140 150 160 170 180 IIHRDIKPEN ILVSQSGITK LCDFGFARTL AAPGDVYTDY VATRWYRAPE LVLKDTTYGK 190 200 210 220 230 240 PVDIWALGCM IIEMATGNPY LPSSSDLDLL HKIVLKVGNL TPHLHNIFSK SPIFAGVVLP 250 260 270 280 290 300 QVQHPKNARK KYPKLNGLLA DIVHACLQID PAERISSTDL LHHDYFTRDG FIEKFIPELR 310 320 330 340 350 360 AKLLQEAKVN SFIKPKENFK ENEPVRDEKK PVFTNPLLYG NPTLYGKEVD RDKRAKELKV 370 380 390 400 410 420 RVIKAKGGKG DVPDLKKTES EGEHRQQGTA EDTHPTSLDR KPSVSELTNP VHPSANSDTV 430 440 450 460 470 480 KEDPHSGGCM IMPPINLTSS NLLAANPSSN LSHPNSRLTE RTKKRRTSSQ TIGQTLSNSR 490 500 510 520 530 540 QEDTGPTQVQ TEKGAFNERT GQNDQIASGN KRKLNFSKCD RKEFHFPELP FTIQAKEMKG 550 560 570 580 590 MEVKQIKVLK RESKKTDSPK IPTLLSMDSN QEKQEVFNIF PGWCKRGNLN WPS
	Isoform 2 (NKIATRE alpha) (p57 alpha isoform) [UniParc]. Checksum: 89747921FF2CE683	FASTA	505	57,243
	10 20 30 40 50 60 MEMYETLGKV GEGSYGTVMK CKHKDTGRIV AIKIFYEKPE KSVNKIATRE IKFLKQFRHE 70 80 90 100 110 120 NLVNLIEVFR QKKKIHLVFE FIDHTVLDEL QHYCHGLESK RLRKYLFQIL RAIEYLHNNN 130 140 150 160 170 180 IIHRDIKPEN ILVSQSGITK LCDFGFARTL AAPGDVYTDY VATRWYRAPE LVLKDTTYGK 190 200 210 220 230 240 PVDIWALGCM IIEMATGNPY LPSSSDLDLL HKIVLKVGNL TPHLHNIFSK SPIFAGVVLP 250 260 270 280 290 300 QVQHPKNARK KYPKLNGLLA DIVHACLQID PAERISSTDL LHHDYFTRDG FIEKFIPELR 310 320 330 340 350 360 AKLLQEAKVN SFIKPKENFK ENEPVRDEKK PVFTNPLLYG NPTLYGKEVD RDKRAKELKV 370 380 390 400 410 420 RVIKAKGGKG DVPDLKKTES EGEHRQQGTA EDTHPTSLDR KPSVSELTNP VHPSANSDTV 430 440 450 460 470 480 KEDPHSGGCM IMPPINLTSS NLLAANPSSN LSHPNSRLTE RTKKRRTSSQ TIGQTLSNSR 490 500 QEDTGPTQVQ TEKGAFNERT GQNAK
	Isoform 3 (NKIATRE beta) (p52 beta isoform) [UniParc]. Checksum: 99D0782794099CDA	FASTA	457	51,895
	10 20 30 40 50 60 MEMYETLGKV GEGSYGTVMK CKHKDTGRIV AIKIFYEKPE KSVNKIATRE IKFLKQFRHE 70 80 90 100 110 120 NLVNLIEVFR QKKKIHLVFE FIDHTVLDEL QHYCHGLESK RLRKYLFQIL RAIEYLHNNN 130 140 150 160 170 180 IIHRDIKPEN ILVSQSGITK LCDFGFARTL AAPGDVYTDY VATRWYRAPE LVLKDTTYGK 190 200 210 220 230 240 PVDIWALGCM IIEMATGNPY LPSSSDLDLL HKIVLKVGNL TPHLHNIFSK SPIFAGVVLP 250 260 270 280 290 300 QVQHPKNARK KYPKLNGLLA DIVHACLQID PAERISSTDL LHHDYFTRDG FIEKFIPELR 310 320 330 340 350 360 AKLLQEAKVN SFIKPKENFK ENEPVRDEKK PVFTNPLLYG NPTLYGKEVD RDKRAKELKV 370 380 390 400 410 420 RVIKAKGGKG DVPDLKKTES EGEHRQQGTA EDTHPTSLDR KPSVSELTNP VHPSANSDTV 430 440 450 KEDPHSGGCM IMPPINLTSS NLLAANPSSN LSHPNSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NKIATRE is a novel conserved cdc2-related kinase." Haq R., Randall S., Midmer M., Yee K., Zanke B. Genomics 71:131-141(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Tissue: Brain and Jejunum.
[2]	"Genome sequence of the Brown Norway rat yields insights into mammalian evolution." Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S. Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF112183 mRNA. Translation: AAF34870.1. AF112184 mRNA. Translation: AAF34871.1. AABR03073386 Genomic DNA. No translation available. AABR03074822 Genomic DNA. No translation available. AABR03073552 Genomic DNA. No translation available.
IPI	IPI00656413. IPI00776875. IPI00957471.
RefSeq	NP_068540.1. NM_021772.2.
UniGene	Rn.154405. Rn.229441.
3D structure databases
ProteinModelPortal	Q9JM01.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000060584.
PTM databases
PhosphoSite	Q9JM01.
Proteomic databases
PaxDb	Q9JM01.
PRIDE	Q9JM01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000059040; ENSRNOP00000055819; ENSRNOG00000005097.
GeneID	60396.
KEGG	rno:60396.
UCSC	RGD:619874. rat.
Organism-specific databases
CTD	51265.
RGD	619874. Cdkl3.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00650000093115.
HOGENOM	HOG000233024.
HOVERGEN	HBG080204.
KO	K08824.
OrthoDB	EOG470TGZ.
Gene expression databases
ArrayExpress	Q9JM01.
Genevestigator	Q9JM01.
GermOnline	ENSRNOG00000005097. Rattus norvegicus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	612104.

Entry information

Entry name

CDKL3_RAT

Accession

Primary (citable) accession number: Q9JM01
Secondary accession number(s): Q9JM02

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	May 1, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program