Q9JLZ6 (HIC2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hypermethylated in cancer 2 protein
Short name=Hic-2

Gene names

Name:	Hic2
Synonyms:	Kiaa1020

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transcriptional repressor By similarity.
Subunit structure	Self-associates. Interacts with HIC1 By similarity.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Hic subfamily. Contains 1 BTB (POZ) domain. Contains 5 C2H2-type zinc fingers.
Sequence caution	The sequence BAC98076.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence CAI30631.1 differs from that shown. Reason: Probable cloning artifact.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	focal adhesion Inferred from electronic annotation. Source: Compara nucleus Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9JLZ6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9JLZ6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     544-619: KMFTQRGTMT...SHLRMHTSPS → SEPTPSCPLQSSGMSGNLQQSQR

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	Hypermethylated in cancer 2 protein		PRO_0000046946
Regions
	Domain	46 – 109	64	BTB
	Zinc finger	446 – 468	23	C2H2-type 1
	Zinc finger	509 – 531	23	C2H2-type 2
	Zinc finger	537 – 559	23	C2H2-type 3
	Zinc finger	565 – 587	23	C2H2-type 4
	Zinc finger	593 – 615	23	C2H2-type 5
	Region	247 – 249	3	Binding to CtBP By similarity
Natural variations
	Alternative sequence	544 – 619	76	KMFTQ…HTSPS → SEPTPSCPLQSSGMSGNLQQ SQR in isoform 2.		VSP_010499
Experimental info
	Sequence conflict	354 – 355	2	RE → AA in AAF28801. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 28, 2011. Version 4. Checksum: AA7E58CC3BA8AB39	FASTA	619	66,766
	10 20 30 40 50 60 MVSGPLALRW CPWAGHRDMG PDMELPSHSK QLLLQLNQQR AKGFLCDVII MVENSIFRAH 70 80 90 100 110 120 KNVLAASSIY FKSLVLHDNL INLDTDMVSS TVFQQILDFI YTGKLLPSDQ PSEPNFSTLL 130 140 150 160 170 180 TAASYLQLPE LAALCRRKLK RAGKPFGPGR VGTAGIGRPT RSQRLSTASV IQARFPGLVD 190 200 210 220 230 240 VRKGHPAPQE LPQAKGSDDE LFLGTSTQES THGLGLGGPA GGEMGLGGCS TSTNGSSGGC 250 260 270 280 290 300 EQELGLDLSK KSPPLPPTTP GPHLTPEDPA QLSDSQRESP APTSTSALPV GNSASFVELG 310 320 330 340 350 360 ATPEEPMDVE GAEENHLSLL EGQGGQPRKS LRHSARKKDW NKKEPVAGSP FDRRETGSKG 370 380 390 400 410 420 SCPGEEGEGT GDRVPNGVLA SSAGGGGPSA SYGEQSFPCK EEEENGKDGS EDSGQSGSEG 430 440 450 460 470 480 GSGHTGAHYV YRQEGYETVS YGDNVYVCIP CAKGFPSSEQ LNAHVETHTE EELFIKEEGA 490 500 510 520 530 540 YETGSGGAEE EAEDLSTPSA AYTADSRPFK CSVCEKTYKD PATLRQHEKT HWLTRPFPCN 550 560 570 580 590 600 ICGKMFTQRG TMTRHMRSHL GLKPFACDEC GMRFTRQYRL TEHMRVHSGE KPYECQLCGG 610 KFTQQRNLIS HLRMHTSPS
	Isoform 2 [UniParc]. Checksum: 7E7110E46948CAF7	FASTA	566	60,187
	10 20 30 40 50 60 MVSGPLALRW CPWAGHRDMG PDMELPSHSK QLLLQLNQQR AKGFLCDVII MVENSIFRAH 70 80 90 100 110 120 KNVLAASSIY FKSLVLHDNL INLDTDMVSS TVFQQILDFI YTGKLLPSDQ PSEPNFSTLL 130 140 150 160 170 180 TAASYLQLPE LAALCRRKLK RAGKPFGPGR VGTAGIGRPT RSQRLSTASV IQARFPGLVD 190 200 210 220 230 240 VRKGHPAPQE LPQAKGSDDE LFLGTSTQES THGLGLGGPA GGEMGLGGCS TSTNGSSGGC 250 260 270 280 290 300 EQELGLDLSK KSPPLPPTTP GPHLTPEDPA QLSDSQRESP APTSTSALPV GNSASFVELG 310 320 330 340 350 360 ATPEEPMDVE GAEENHLSLL EGQGGQPRKS LRHSARKKDW NKKEPVAGSP FDRRETGSKG 370 380 390 400 410 420 SCPGEEGEGT GDRVPNGVLA SSAGGGGPSA SYGEQSFPCK EEEENGKDGS EDSGQSGSEG 430 440 450 460 470 480 GSGHTGAHYV YRQEGYETVS YGDNVYVCIP CAKGFPSSEQ LNAHVETHTE EELFIKEEGA 490 500 510 520 530 540 YETGSGGAEE EAEDLSTPSA AYTADSRPFK CSVCEKTYKD PATLRQHEKT HWLTRPFPCN 550 560 ICGSEPTPSC PLQSSGMSGN LQQSQR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization and expression analysis of the murine Hic2 gene." Terzic A., Graw J. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: C3H.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J and NOD. Tissue: Embryo, Lung and Spleen.
[3]	"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H. DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Embryonic tail.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
[5]	"Hic2, a novel homolog of Hic1 and gammaFBP." Grimm C., Graw J. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 354-619 (ISOFORM 1). Strain: Swiss Webster. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ868292 mRNA. Translation: CAI30631.1. Sequence problems. AK084975 mRNA. Translation: BAC39326.1. AK145386 mRNA. Translation: BAE26404.1. AK156332 mRNA. Translation: BAE33679.1. AK157276 mRNA. Translation: BAE34025.1. AK129266 mRNA. Translation: BAC98076.1. Different initiation. BC065124 mRNA. Translation: AAH65124.1. AF117382 mRNA. Translation: AAF28801.1.
IPI	IPI00267297. IPI00752014.
RefSeq	NP_849253.2. NM_178922.3.
UniGene	Mm.261602.
3D structure databases
ProteinModelPortal	Q9JLZ6.
SMR	Q9JLZ6. Positions 23-135, 446-617.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4097465.
PTM databases
PhosphoSite	Q9JLZ6.
Proteomic databases
PaxDb	Q9JLZ6.
PRIDE	Q9JLZ6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000090190; ENSMUSP00000087656; ENSMUSG00000050240. ENSMUST00000115698; ENSMUSP00000111362; ENSMUSG00000050240.
GeneID	58180.
KEGG	mmu:58180.
UCSC	uc007yko.1. mouse.
Organism-specific databases
CTD	23119.
MGI	MGI:1929869. Hic2.
Rouge	Search...
Phylogenomic databases
eggNOG	COG5048.
GeneTree	ENSGT00700000104525.
HOGENOM	HOG000026793.
HOVERGEN	HBG079492.
InParanoid	Q9JLZ6.
OMA	VCIPCAK.
OrthoDB	EOG4BCDMT.
Gene expression databases
Bgee	Q9JLZ6.
CleanEx	MM_HIC2.
Genevestigator	Q9JLZ6.
GermOnline	ENSMUSG00000050240. Mus musculus.
Family and domain databases
Gene3D	3.30.160.60. 5 hits. 3.30.710.10. 1 hit.
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR013069. BTB_POZ. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF00651. BTB. 1 hit. PF00096. zf-C2H2. 1 hit. [Graphical view]
SMART	SM00225. BTB. 1 hit. SM00355. ZnF_C2H2. 5 hits. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit.
PROSITE	PS50097. BTB. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 5 hits. PS50157. ZINC_FINGER_C2H2_2. 5 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	314131.
SOURCE	Search...

Entry information

Entry name

HIC2_MOUSE

Accession

Primary (citable) accession number: Q9JLZ6
Secondary accession number(s): Q3U030 , Q3ULP4, Q5K036, Q8BSZ9, Q8C3T5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	June 28, 2011
Last modified:	May 29, 2013
This is version 112 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program