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Protein

Glutaredoxin-3

Gene

Glrx3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Together with BOLA2, acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins (By similarity). Acts as a critical negative regulator of cardiac hypertrophy and a positive inotropic regulator (PubMed:16809552, PubMed:18258855). Required for hemoglobin maturation (By similarity). Does not possess any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity (By similarity).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi161Iron-sulfur (2Fe-2S); shared with dimeric partnerBy similarity1
Metal bindingi263Iron-sulfur (2Fe-2S); shared with dimeric partnerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandIron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaredoxin-3
Alternative name(s):
PKC-interacting cousin of thioredoxin
Short name:
PICOT
PKC-theta-interacting protein
Short name:
PKCq-interacting protein
Thioredoxin-like protein 2
Gene namesi
Name:Glrx3
Synonyms:Picot, Txnl2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi69414 Glrx3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001200212 – 337Glutaredoxin-3Add BLAST336

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei119PhosphoserineBy similarity1
Modified residuei122PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9JLZ1
PRIDEiQ9JLZ1

PTM databases

iPTMnetiQ9JLZ1
PhosphoSitePlusiQ9JLZ1

Expressioni

Inductioni

In neonatal cardiomyocytes after exposure to the hypertrophic agonists EDN1 (ET-1) or phenylephrine (PE). In transverse aortic constriction (TAC) induced cardiac hypertrophy in adult hearts.1 Publication

Gene expression databases

BgeeiENSRNOG00000016227
ExpressionAtlasiQ9JLZ1 baseline and differential
GenevisibleiQ9JLZ1 RN

Interactioni

Subunit structurei

Homodimer; the homodimer is independent of 2Fe-2S clusters. Heterotrimer; forms a heterotrimeric complex composed by two BOLA2 molecules and one GLRX3 molecule; linked by [2Fe-2S] clusters. Interacts (via N-terminus) with PRKCQ/PKC-theta (By similarity). Interacts (via C-terminus) with CSRP3 (PubMed:18258855). Interacts with CSRP2 (By similarity).By similarity1 Publication

GO - Molecular functioni

  • identical protein binding Source: Ensembl
  • protein kinase C binding Source: RGD

Protein-protein interaction databases

IntActiQ9JLZ1, 1 interactor
STRINGi10116.ENSRNOP00000022406

Structurei

3D structure databases

ProteinModelPortaliQ9JLZ1
SMRiQ9JLZ1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 119ThioredoxinPROSITE-ProRule annotationAdd BLAST118
Domaini144 – 238Glutaredoxin 1PROSITE-ProRule annotationAdd BLAST95
Domaini239 – 337Glutaredoxin 2PROSITE-ProRule annotationAdd BLAST99

Domaini

The thioredoxin domain lacks the two redox-active cysteines. This strongly suggests that it lacks thioredoxin activity.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0911 Eukaryota
COG0278 LUCA
GeneTreeiENSGT00550000075030
HOGENOMiHOG000165751
HOVERGENiHBG054719
InParanoidiQ9JLZ1
OMAiNNWPTFP
OrthoDBiEOG091G0YN0
PhylomeDBiQ9JLZ1
TreeFamiTF314151

Family and domain databases

CDDicd03028 GRX_PICOT_like, 2 hits
InterProiView protein in InterPro
IPR002109 Glutaredoxin
IPR033658 GRX_PICOT-like
IPR004480 Monothiol_GRX-rel
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PANTHERiPTHR10293 PTHR10293, 1 hit
PfamiView protein in Pfam
PF00462 Glutaredoxin, 2 hits
PF00085 Thioredoxin, 1 hit
SUPFAMiSSF52833 SSF52833, 3 hits
TIGRFAMsiTIGR00365 TIGR00365, 1 hit
PROSITEiView protein in PROSITE
PS51354 GLUTAREDOXIN_2, 2 hits
PS51352 THIOREDOXIN_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JLZ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGAAEAAE AAVAVVEVGS ARQFEELLRL KTKSLLVVHF WAPWAPQCVQ
60 70 80 90 100
MNDVMAELAK EHPHVSFVKL EAEAVPEVSE KYEISSVPTF LFFKNSQKVD
110 120 130 140 150
RLDGAHAPEL TKKVQRHVSS GSFPPSTNEH VKEDLNLRLK KLTHAAPCML
160 170 180 190 200
FMKGTPQEPR CGFSKQMVEI LHKHNIQFSS FDIFSDEEVR QGLKTYSNWP
210 220 230 240 250
TYPQLYVSGE LIGGLDIIKE LEASEELDTI CPKAPKLEER LKVLTNKASV
260 270 280 290 300
MLFMKGNKQE AKCGFSKQIL EILNSTGVEY ETFDILEDEE VRQGLKTFSN
310 320 330
WPTYPQLYVR GDLVGGLDIV KELKDNGELL PILKGEN
Length:337
Mass (Da):37,849
Last modified:March 1, 2005 - v2
Checksum:iDA1E3DA9C0DBC22C
GO
Isoform 2 (identifier: Q9JLZ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     94-151: Missing.

Show »
Length:279
Mass (Da):31,361
Checksum:iDD56FE9EB5D1DA76
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01292794 – 151Missing in isoform 2. 1 PublicationAdd BLAST58

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF118651 mRNA Translation: AAF28843.1
BC086381 mRNA Translation: AAH86381.1
RefSeqiNP_116003.2, NM_032614.2 [Q9JLZ1-1]
UniGeneiRn.3578

Genome annotation databases

EnsembliENSRNOT00000022406; ENSRNOP00000022406; ENSRNOG00000016227 [Q9JLZ1-1]
GeneIDi58815
KEGGirno:58815
UCSCiRGD:69414 rat [Q9JLZ1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGLRX3_RAT
AccessioniPrimary (citable) accession number: Q9JLZ1
Secondary accession number(s): Q5RK11
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: March 1, 2005
Last modified: May 23, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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