Q9JLV2 (TP4AP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Short transient receptor potential channel 4-associated protein
Short name=Trp4-associated protein
Short name=Trpc4-associated protein

Alternative name(s):
Protein TAP1
Rabex-5/Rin2-interacting protein
TNF-receptor ubiquitous scaffolding/signaling protein
Short name=Protein TRUSS

Gene names

Name:	Trpc4ap
Synonyms:	Trrp4ap

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	797 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(TRUSS) complex specifically mediates the polyubiquitination and subsequent degradation of MYC By similarity. Also participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. Involved in JNK activation via its interaction with TRAF2. Also involved in elevation of endoplasmic reticulum Ca2+ storage reduction in response to CHRM1. Ref.5 Ref.6
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Component of the DCX(TRUSS) E3 ubiquitin ligase complex, at least composed of CUL4A, DDB1, TRPC4AP/TRUSS and RBX1. Interacts with MYC By similarity. Constitutively associated with TNFRSF1A. Directly interacts with TRADD, TRAF2, CHUK, IKBKB and IKBKG. Interacts with TRPC1, TRPC4 and TRPC5. Ref.5 Ref.6
Tissue specificity	Widely expressed, with high levels in heart, liver and testis.
Sequence caution	The sequence AAL08422.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	Cul4A-RING ubiquitin ligase complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	phosphatase binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9JLV2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9JLV2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     351-358: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 797	797	Short transient receptor potential channel 4-associated protein		PRO_0000072642
Regions
	Region	1 – 400	400	Interaction with TNFRSF1A
Natural variations
	Alternative sequence	351 – 358	8	Missing in isoform 2.		VSP_003983
Experimental info
	Sequence conflict	11 – 14	4	GASR → LPYW in AAF36513. Ref.2
	Sequence conflict	158	1	I → L in AAF36513. Ref.2
	Sequence conflict	193	1	F → L in AAF36513. Ref.2
	Sequence conflict	359	1	G → S in AAM46938. Ref.1
	Sequence conflict	532	1	R → K in AAM46938. Ref.1
	Sequence conflict	769	1	L → V in AAL08422. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 15, 2002. Version 2. Checksum: 8BC3453A7658A234	FASTA	797	90,727
	10 20 30 40 50 60 MAAAPAAAGA GASRGRRLAA TAAAWGGWGG RPRPGNILLQ LRQGQLTGRG LVRAVQFTET 70 80 90 100 110 120 FLTERDKLSK WSGIPQLLLK LYATSHLHSD FVECQSILKE ISPLLSMEAM AFVTEDRKFT 130 140 150 160 170 180 QEATYPNTYI FDLFGGVDLL VEILMRPTIS IRGQKLKISD EMSKDCLSIL YNTCVCTEGV 190 200 210 220 230 240 TKRLAEKNDF VIFLFTLMTS KKTFLQTATL IEDILGVKKE MIRLDEVPNL SSLVSNFDQQ 250 260 270 280 290 300 QLANFCRILA VTISEMDTGN DDKHTLLAKN AQQKKSLSLG PSAAEINQAA LLSIPGFVER 310 320 330 340 350 360 LCKLATRKVS ESTGTASFLQ ELEEWYTWLD NALVLDALMR VANEESEHNQ APTVFPSLGT 370 380 390 400 410 420 SEEGGLPHTS ARAQLPQSMK IMHEIMYKLE VLYVLCVLLM GRQRNQVHRM IAEFKLIPGL 430 440 450 460 470 480 NNLFDKLIWR KHSASALVLH GHNQNCDCSP DITLKIQFLR LLQSFSDHHE NKYLLLNNQE 490 500 510 520 530 540 LNELSAISLK ANIPEVEAVL NTDRSLVCDG KRGLLTRLLQ VMKKEPAESS FRFWQARAVE 550 560 570 580 590 600 SFLRGTTSYA DQMFLLKRGL LEHILYCIVD SECKSRDVLQ SYFDLLGELM KFNVDAFKRF 610 620 630 640 650 660 NKYINTDAKF QVFLKQINSS LVDSNMLVRC VTLSLDRFEN QVDMKVAEVL SECRLLAYIS 670 680 690 700 710 720 QVPTQMSFLF RLINIIHVQT LTQENVSCLN TSLVILMLAR RKERLPLYLR LLQRMEHSKK 730 740 750 760 770 780 YPGFLLNNFH NLLRFWQQHY LHKDKDSTCL ENSSCISFSY WKETVSILLN PDRQSPSALV 790 SYIEEPYMDI DRDFTEE
	Isoform 2 [UniParc]. Checksum: 92D659B52F87206E	FASTA	789	89,914
	10 20 30 40 50 60 MAAAPAAAGA GASRGRRLAA TAAAWGGWGG RPRPGNILLQ LRQGQLTGRG LVRAVQFTET 70 80 90 100 110 120 FLTERDKLSK WSGIPQLLLK LYATSHLHSD FVECQSILKE ISPLLSMEAM AFVTEDRKFT 130 140 150 160 170 180 QEATYPNTYI FDLFGGVDLL VEILMRPTIS IRGQKLKISD EMSKDCLSIL YNTCVCTEGV 190 200 210 220 230 240 TKRLAEKNDF VIFLFTLMTS KKTFLQTATL IEDILGVKKE MIRLDEVPNL SSLVSNFDQQ 250 260 270 280 290 300 QLANFCRILA VTISEMDTGN DDKHTLLAKN AQQKKSLSLG PSAAEINQAA LLSIPGFVER 310 320 330 340 350 360 LCKLATRKVS ESTGTASFLQ ELEEWYTWLD NALVLDALMR VANEESEHNQ GTSEEGGLPH 370 380 390 400 410 420 TSARAQLPQS MKIMHEIMYK LEVLYVLCVL LMGRQRNQVH RMIAEFKLIP GLNNLFDKLI 430 440 450 460 470 480 WRKHSASALV LHGHNQNCDC SPDITLKIQF LRLLQSFSDH HENKYLLLNN QELNELSAIS 490 500 510 520 530 540 LKANIPEVEA VLNTDRSLVC DGKRGLLTRL LQVMKKEPAE SSFRFWQARA VESFLRGTTS 550 560 570 580 590 600 YADQMFLLKR GLLEHILYCI VDSECKSRDV LQSYFDLLGE LMKFNVDAFK RFNKYINTDA 610 620 630 640 650 660 KFQVFLKQIN SSLVDSNMLV RCVTLSLDRF ENQVDMKVAE VLSECRLLAY ISQVPTQMSF 670 680 690 700 710 720 LFRLINIIHV QTLTQENVSC LNTSLVILML ARRKERLPLY LRLLQRMEHS KKYPGFLLNN 730 740 750 760 770 780 FHNLLRFWQQ HYLHKDKDST CLENSSCISF SYWKETVSIL LNPDRQSPSA LVSYIEEPYM DIDRDFTEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TRUSS, a novel tumor necrosis factor receptor 1 scaffolding protein that mediates activation of the transcription factor NF-kappaB." Soond S.M., Terry J.L., Colbert J.D., Riches D.W.H. Mol. Cell. Biol. 23:8334-8344(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: BALB/c.
[2]	Qian F., Philipson L.H. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Insulinoma.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Mammary gland.
[4]	"A novel Rabex-5/Rin2-interacting protein." Scherrer D., Tsai M., Galli S.J., Tam S.-Y. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 154-797 (ISOFORM 1). Strain: BALB/c. Tissue: Brain.
[5]	"TRUSS, a tumor necrosis factor receptor-1-interacting protein, activates c-Jun NH(2)-terminal kinase and transcription factor AP-1." Soond S.M., Terry J.L., Riches D.W.H. FEBS Lett. 580:4591-4596(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH TRAF2.
[6]	"TRUSS, TNF-R1, and TRPC ion channels synergistically reverse endoplasmic reticulum Ca2+ storage reduction in response to m1 muscarinic acetylcholine receptor signaling." Mace K.E., Lussier M.P., Boulay G., Terry-Powers J.L., Parfrey H., Perraud A.L., Riches D.W.H. J. Cell. Physiol. 225:444-453(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH TRPC1; TRPC4 AND TRPC5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF509881 mRNA. Translation: AAM46938.1. AF130458 mRNA. Translation: AAF36513.1. BC003931 mRNA. Translation: AAH03931.1. BC033274 mRNA. Translation: AAH33274.1. BC057330 mRNA. Translation: AAH57330.1. AF093589 mRNA. Translation: AAL08422.1. Different initiation.
IPI	IPI00124751. IPI00230169.
RefSeq	NP_001156924.1. NM_001163452.1. NP_062802.2. NM_019828.2.
UniGene	Mm.268304. Mm.469980.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000037574.
PTM databases
PhosphoSite	Q9JLV2.
Proteomic databases
PaxDb	Q9JLV2.
PRIDE	Q9JLV2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000041059; ENSMUSP00000037574; ENSMUSG00000038324. ENSMUST00000103140; ENSMUSP00000099429; ENSMUSG00000038324.
GeneID	56407.
KEGG	mmu:56407.
Organism-specific databases
CTD	26133.
MGI	MGI:1930751. Trpc4ap.
Phylogenomic databases
eggNOG	NOG42180.
GeneTree	ENSGT00390000018330.
HOGENOM	HOG000231794.
HOVERGEN	HBG059886.
InParanoid	Q9JLV2.
KO	K11796.
OMA	QESTFPN.
OrthoDB	EOG49KFQ0.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q9JLV2.
Bgee	Q9JLV2.
Genevestigator	Q9JLV2.
GermOnline	ENSMUSG00000038324. Mus musculus.
Family and domain databases
InterPro	IPR022162. DUF3689. [Graphical view]
Pfam	PF12463. DUF3689. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TRPC4AP. mouse.
NextBio	312538.
SOURCE	Search...

Entry information

Entry name

TP4AP_MOUSE

Accession

Primary (citable) accession number: Q9JLV2
Secondary accession number(s): Q920J6, Q99L03

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	November 15, 2002
Last modified:	April 3, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program