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Q9JLV2

- TP4AP_MOUSE

UniProt

Q9JLV2 - TP4AP_MOUSE

Protein

Short transient receptor potential channel 4-associated protein

Gene

Trpc4ap

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 2 (15 Nov 2002)
      Previous versions | rss
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    Functioni

    Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(TRUSS) complex specifically mediates the polyubiquitination and subsequent degradation of MYC By similarity. Also participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. Involved in JNK activation via its interaction with TRAF2. Also involved in elevation of endoplasmic reticulum Ca2+ storage reduction in response to CHRM1.By similarity2 Publications

    Pathwayi

    GO - Molecular functioni

    1. phosphatase binding Source: UniProtKB

    GO - Biological processi

    1. protein ubiquitination Source: UniProtKB
    2. ubiquitin-dependent protein catabolic process Source: UniProtKB

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    ReactomeiREACT_227861. TRP channels.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Short transient receptor potential channel 4-associated protein
    Short name:
    Trp4-associated protein
    Short name:
    Trpc4-associated protein
    Alternative name(s):
    Protein TAP1
    Rabex-5/Rin2-interacting protein
    TNF-receptor ubiquitous scaffolding/signaling protein
    Short name:
    Protein TRUSS
    Gene namesi
    Name:Trpc4ap
    Synonyms:Trrp4ap
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1930751. Trpc4ap.

    Subcellular locationi

    GO - Cellular componenti

    1. Cul4A-RING E3 ubiquitin ligase complex Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 797796Short transient receptor potential channel 4-associated proteinPRO_0000072642Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ9JLV2.
    PRIDEiQ9JLV2.

    PTM databases

    PhosphoSiteiQ9JLV2.

    Expressioni

    Tissue specificityi

    Widely expressed, with high levels in heart, liver and testis.

    Gene expression databases

    BgeeiQ9JLV2.
    GenevestigatoriQ9JLV2.

    Interactioni

    Subunit structurei

    Component of the DCX(TRUSS) E3 ubiquitin ligase complex, at least composed of CUL4A, DDB1, TRPC4AP/TRUSS and RBX1. Interacts with MYC By similarity. Constitutively associated with TNFRSF1A. Directly interacts with TRADD, TRAF2, CHUK, IKBKB and IKBKG. Interacts with TRPC1, TRPC4 and TRPC5.By similarity2 Publications

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000037574.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 400399Interaction with TNFRSF1AAdd
    BLAST

    Phylogenomic databases

    eggNOGiNOG42180.
    GeneTreeiENSGT00390000018330.
    HOGENOMiHOG000231794.
    HOVERGENiHBG059886.
    InParanoidiQ9JLV2.
    KOiK11796.
    OMAiHYLNKDK.
    OrthoDBiEOG7P02H9.
    TreeFamiTF329145.

    Family and domain databases

    InterProiIPR016024. ARM-type_fold.
    IPR022162. DUF3689.
    [Graphical view]
    PfamiPF12463. DUF3689. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 4 hits.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9JLV2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAAPAAAGA GASRGRRLAA TAAAWGGWGG RPRPGNILLQ LRQGQLTGRG    50
    LVRAVQFTET FLTERDKLSK WSGIPQLLLK LYATSHLHSD FVECQSILKE 100
    ISPLLSMEAM AFVTEDRKFT QEATYPNTYI FDLFGGVDLL VEILMRPTIS 150
    IRGQKLKISD EMSKDCLSIL YNTCVCTEGV TKRLAEKNDF VIFLFTLMTS 200
    KKTFLQTATL IEDILGVKKE MIRLDEVPNL SSLVSNFDQQ QLANFCRILA 250
    VTISEMDTGN DDKHTLLAKN AQQKKSLSLG PSAAEINQAA LLSIPGFVER 300
    LCKLATRKVS ESTGTASFLQ ELEEWYTWLD NALVLDALMR VANEESEHNQ 350
    APTVFPSLGT SEEGGLPHTS ARAQLPQSMK IMHEIMYKLE VLYVLCVLLM 400
    GRQRNQVHRM IAEFKLIPGL NNLFDKLIWR KHSASALVLH GHNQNCDCSP 450
    DITLKIQFLR LLQSFSDHHE NKYLLLNNQE LNELSAISLK ANIPEVEAVL 500
    NTDRSLVCDG KRGLLTRLLQ VMKKEPAESS FRFWQARAVE SFLRGTTSYA 550
    DQMFLLKRGL LEHILYCIVD SECKSRDVLQ SYFDLLGELM KFNVDAFKRF 600
    NKYINTDAKF QVFLKQINSS LVDSNMLVRC VTLSLDRFEN QVDMKVAEVL 650
    SECRLLAYIS QVPTQMSFLF RLINIIHVQT LTQENVSCLN TSLVILMLAR 700
    RKERLPLYLR LLQRMEHSKK YPGFLLNNFH NLLRFWQQHY LHKDKDSTCL 750
    ENSSCISFSY WKETVSILLN PDRQSPSALV SYIEEPYMDI DRDFTEE 797
    Length:797
    Mass (Da):90,727
    Last modified:November 15, 2002 - v2
    Checksum:i8BC3453A7658A234
    GO
    Isoform 2 (identifier: Q9JLV2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         351-358: Missing.

    Show »
    Length:789
    Mass (Da):89,914
    Checksum:i92D659B52F87206E
    GO

    Sequence cautioni

    The sequence AAL08422.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti11 – 144GASR → LPYW in AAF36513. 1 PublicationCurated
    Sequence conflicti158 – 1581I → L in AAF36513. 1 PublicationCurated
    Sequence conflicti193 – 1931F → L in AAF36513. 1 PublicationCurated
    Sequence conflicti359 – 3591G → S in AAM46938. (PubMed:14585990)Curated
    Sequence conflicti532 – 5321R → K in AAM46938. (PubMed:14585990)Curated
    Sequence conflicti769 – 7691L → V in AAL08422. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei351 – 3588Missing in isoform 2. 1 PublicationVSP_003983

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF509881 mRNA. Translation: AAM46938.1.
    AF130458 mRNA. Translation: AAF36513.1.
    BC003931 mRNA. Translation: AAH03931.1.
    BC033274 mRNA. Translation: AAH33274.1.
    BC057330 mRNA. Translation: AAH57330.1.
    AF093589 mRNA. Translation: AAL08422.1. Different initiation.
    CCDSiCCDS16952.1. [Q9JLV2-2]
    CCDS50769.1. [Q9JLV2-1]
    RefSeqiNP_001156924.1. NM_001163452.1. [Q9JLV2-1]
    NP_062802.2. NM_019828.2. [Q9JLV2-2]
    UniGeneiMm.268304.
    Mm.469980.

    Genome annotation databases

    EnsembliENSMUST00000041059; ENSMUSP00000037574; ENSMUSG00000038324. [Q9JLV2-1]
    ENSMUST00000103140; ENSMUSP00000099429; ENSMUSG00000038324. [Q9JLV2-2]
    GeneIDi56407.
    KEGGimmu:56407.
    UCSCiuc008nlc.2. mouse. [Q9JLV2-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF509881 mRNA. Translation: AAM46938.1 .
    AF130458 mRNA. Translation: AAF36513.1 .
    BC003931 mRNA. Translation: AAH03931.1 .
    BC033274 mRNA. Translation: AAH33274.1 .
    BC057330 mRNA. Translation: AAH57330.1 .
    AF093589 mRNA. Translation: AAL08422.1 . Different initiation.
    CCDSi CCDS16952.1. [Q9JLV2-2 ]
    CCDS50769.1. [Q9JLV2-1 ]
    RefSeqi NP_001156924.1. NM_001163452.1. [Q9JLV2-1 ]
    NP_062802.2. NM_019828.2. [Q9JLV2-2 ]
    UniGenei Mm.268304.
    Mm.469980.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000037574.

    PTM databases

    PhosphoSitei Q9JLV2.

    Proteomic databases

    PaxDbi Q9JLV2.
    PRIDEi Q9JLV2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000041059 ; ENSMUSP00000037574 ; ENSMUSG00000038324 . [Q9JLV2-1 ]
    ENSMUST00000103140 ; ENSMUSP00000099429 ; ENSMUSG00000038324 . [Q9JLV2-2 ]
    GeneIDi 56407.
    KEGGi mmu:56407.
    UCSCi uc008nlc.2. mouse. [Q9JLV2-1 ]

    Organism-specific databases

    CTDi 26133.
    MGIi MGI:1930751. Trpc4ap.

    Phylogenomic databases

    eggNOGi NOG42180.
    GeneTreei ENSGT00390000018330.
    HOGENOMi HOG000231794.
    HOVERGENi HBG059886.
    InParanoidi Q9JLV2.
    KOi K11796.
    OMAi HYLNKDK.
    OrthoDBi EOG7P02H9.
    TreeFami TF329145.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_227861. TRP channels.

    Miscellaneous databases

    ChiTaRSi TRPC4AP. mouse.
    NextBioi 312538.
    PROi Q9JLV2.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9JLV2.
    Genevestigatori Q9JLV2.

    Family and domain databases

    InterProi IPR016024. ARM-type_fold.
    IPR022162. DUF3689.
    [Graphical view ]
    Pfami PF12463. DUF3689. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 4 hits.
    ProtoNeti Search...

    Publicationsi

    1. "TRUSS, a novel tumor necrosis factor receptor 1 scaffolding protein that mediates activation of the transcription factor NF-kappaB."
      Soond S.M., Terry J.L., Colbert J.D., Riches D.W.H.
      Mol. Cell. Biol. 23:8334-8344(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: BALB/c.
    2. Qian F., Philipson L.H.
      Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Insulinoma.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Mammary gland.
    4. "A novel Rabex-5/Rin2-interacting protein."
      Scherrer D., Tsai M., Galli S.J., Tam S.-Y.
      Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 154-797 (ISOFORM 1).
      Strain: BALB/c.
      Tissue: Brain.
    5. "TRUSS, a tumor necrosis factor receptor-1-interacting protein, activates c-Jun NH(2)-terminal kinase and transcription factor AP-1."
      Soond S.M., Terry J.L., Riches D.W.H.
      FEBS Lett. 580:4591-4596(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TRAF2.
    6. "TRUSS, TNF-R1, and TRPC ion channels synergistically reverse endoplasmic reticulum Ca2+ storage reduction in response to m1 muscarinic acetylcholine receptor signaling."
      Mace K.E., Lussier M.P., Boulay G., Terry-Powers J.L., Parfrey H., Perraud A.L., Riches D.W.H.
      J. Cell. Physiol. 225:444-453(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TRPC1; TRPC4 AND TRPC5.

    Entry informationi

    Entry nameiTP4AP_MOUSE
    AccessioniPrimary (citable) accession number: Q9JLV2
    Secondary accession number(s): Q920J6, Q99L03
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 15, 2002
    Last sequence update: November 15, 2002
    Last modified: October 1, 2014
    This is version 108 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways

    External Data

    Dasty 3