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Q9JLV2

- TP4AP_MOUSE

UniProt

Q9JLV2 - TP4AP_MOUSE

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Protein

Short transient receptor potential channel 4-associated protein

Gene

Trpc4ap

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(TRUSS) complex specifically mediates the polyubiquitination and subsequent degradation of MYC (By similarity). Also participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. Involved in JNK activation via its interaction with TRAF2. Also involved in elevation of endoplasmic reticulum Ca2+ storage reduction in response to CHRM1.By similarity2 Publications

Pathwayi

GO - Molecular functioni

  1. phosphatase binding Source: UniProtKB

GO - Biological processi

  1. protein ubiquitination Source: UniProtKB
  2. ubiquitin-dependent protein catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_227861. TRP channels.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Short transient receptor potential channel 4-associated protein
Short name:
Trp4-associated protein
Short name:
Trpc4-associated protein
Alternative name(s):
Protein TAP1
Rabex-5/Rin2-interacting protein
TNF-receptor ubiquitous scaffolding/signaling protein
Short name:
Protein TRUSS
Gene namesi
Name:Trpc4ap
Synonyms:Trrp4ap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1930751. Trpc4ap.

Subcellular locationi

GO - Cellular componenti

  1. Cul4A-RING E3 ubiquitin ligase complex Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 797796Short transient receptor potential channel 4-associated proteinPRO_0000072642Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9JLV2.
PaxDbiQ9JLV2.
PRIDEiQ9JLV2.

PTM databases

PhosphoSiteiQ9JLV2.

Expressioni

Tissue specificityi

Widely expressed, with high levels in heart, liver and testis.

Gene expression databases

BgeeiQ9JLV2.
ExpressionAtlasiQ9JLV2. baseline.
GenevestigatoriQ9JLV2.

Interactioni

Subunit structurei

Component of the DCX(TRUSS) E3 ubiquitin ligase complex, at least composed of CUL4A, DDB1, TRPC4AP/TRUSS and RBX1. Interacts with MYC (By similarity). Constitutively associated with TNFRSF1A. Directly interacts with TRADD, TRAF2, CHUK, IKBKB and IKBKG. Interacts with TRPC1, TRPC4 and TRPC5.By similarity2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037574.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 400399Interaction with TNFRSF1AAdd
BLAST

Phylogenomic databases

eggNOGiNOG42180.
GeneTreeiENSGT00390000018330.
HOGENOMiHOG000231794.
HOVERGENiHBG059886.
InParanoidiQ9JLV2.
KOiK11796.
OMAiHYLNKDK.
OrthoDBiEOG7P02H9.
TreeFamiTF329145.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR022162. DUF3689.
[Graphical view]
PfamiPF12463. DUF3689. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 4 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9JLV2-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAPAAAGA GASRGRRLAA TAAAWGGWGG RPRPGNILLQ LRQGQLTGRG
60 70 80 90 100
LVRAVQFTET FLTERDKLSK WSGIPQLLLK LYATSHLHSD FVECQSILKE
110 120 130 140 150
ISPLLSMEAM AFVTEDRKFT QEATYPNTYI FDLFGGVDLL VEILMRPTIS
160 170 180 190 200
IRGQKLKISD EMSKDCLSIL YNTCVCTEGV TKRLAEKNDF VIFLFTLMTS
210 220 230 240 250
KKTFLQTATL IEDILGVKKE MIRLDEVPNL SSLVSNFDQQ QLANFCRILA
260 270 280 290 300
VTISEMDTGN DDKHTLLAKN AQQKKSLSLG PSAAEINQAA LLSIPGFVER
310 320 330 340 350
LCKLATRKVS ESTGTASFLQ ELEEWYTWLD NALVLDALMR VANEESEHNQ
360 370 380 390 400
APTVFPSLGT SEEGGLPHTS ARAQLPQSMK IMHEIMYKLE VLYVLCVLLM
410 420 430 440 450
GRQRNQVHRM IAEFKLIPGL NNLFDKLIWR KHSASALVLH GHNQNCDCSP
460 470 480 490 500
DITLKIQFLR LLQSFSDHHE NKYLLLNNQE LNELSAISLK ANIPEVEAVL
510 520 530 540 550
NTDRSLVCDG KRGLLTRLLQ VMKKEPAESS FRFWQARAVE SFLRGTTSYA
560 570 580 590 600
DQMFLLKRGL LEHILYCIVD SECKSRDVLQ SYFDLLGELM KFNVDAFKRF
610 620 630 640 650
NKYINTDAKF QVFLKQINSS LVDSNMLVRC VTLSLDRFEN QVDMKVAEVL
660 670 680 690 700
SECRLLAYIS QVPTQMSFLF RLINIIHVQT LTQENVSCLN TSLVILMLAR
710 720 730 740 750
RKERLPLYLR LLQRMEHSKK YPGFLLNNFH NLLRFWQQHY LHKDKDSTCL
760 770 780 790
ENSSCISFSY WKETVSILLN PDRQSPSALV SYIEEPYMDI DRDFTEE
Length:797
Mass (Da):90,727
Last modified:November 15, 2002 - v2
Checksum:i8BC3453A7658A234
GO
Isoform 2 (identifier: Q9JLV2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     351-358: Missing.

Show »
Length:789
Mass (Da):89,914
Checksum:i92D659B52F87206E
GO

Sequence cautioni

The sequence AAL08422.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 144GASR → LPYW in AAF36513. 1 PublicationCurated
Sequence conflicti158 – 1581I → L in AAF36513. 1 PublicationCurated
Sequence conflicti193 – 1931F → L in AAF36513. 1 PublicationCurated
Sequence conflicti359 – 3591G → S in AAM46938. (PubMed:14585990)Curated
Sequence conflicti532 – 5321R → K in AAM46938. (PubMed:14585990)Curated
Sequence conflicti769 – 7691L → V in AAL08422. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei351 – 3588Missing in isoform 2. 1 PublicationVSP_003983

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF509881 mRNA. Translation: AAM46938.1.
AF130458 mRNA. Translation: AAF36513.1.
BC003931 mRNA. Translation: AAH03931.1.
BC033274 mRNA. Translation: AAH33274.1.
BC057330 mRNA. Translation: AAH57330.1.
AF093589 mRNA. Translation: AAL08422.1. Different initiation.
CCDSiCCDS16952.1. [Q9JLV2-2]
CCDS50769.1. [Q9JLV2-1]
RefSeqiNP_001156924.1. NM_001163452.1. [Q9JLV2-1]
NP_062802.2. NM_019828.2. [Q9JLV2-2]
UniGeneiMm.268304.
Mm.469980.

Genome annotation databases

EnsembliENSMUST00000041059; ENSMUSP00000037574; ENSMUSG00000038324. [Q9JLV2-1]
ENSMUST00000103140; ENSMUSP00000099429; ENSMUSG00000038324. [Q9JLV2-2]
GeneIDi56407.
KEGGimmu:56407.
UCSCiuc008nlc.2. mouse. [Q9JLV2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF509881 mRNA. Translation: AAM46938.1 .
AF130458 mRNA. Translation: AAF36513.1 .
BC003931 mRNA. Translation: AAH03931.1 .
BC033274 mRNA. Translation: AAH33274.1 .
BC057330 mRNA. Translation: AAH57330.1 .
AF093589 mRNA. Translation: AAL08422.1 . Different initiation.
CCDSi CCDS16952.1. [Q9JLV2-2 ]
CCDS50769.1. [Q9JLV2-1 ]
RefSeqi NP_001156924.1. NM_001163452.1. [Q9JLV2-1 ]
NP_062802.2. NM_019828.2. [Q9JLV2-2 ]
UniGenei Mm.268304.
Mm.469980.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000037574.

PTM databases

PhosphoSitei Q9JLV2.

Proteomic databases

MaxQBi Q9JLV2.
PaxDbi Q9JLV2.
PRIDEi Q9JLV2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000041059 ; ENSMUSP00000037574 ; ENSMUSG00000038324 . [Q9JLV2-1 ]
ENSMUST00000103140 ; ENSMUSP00000099429 ; ENSMUSG00000038324 . [Q9JLV2-2 ]
GeneIDi 56407.
KEGGi mmu:56407.
UCSCi uc008nlc.2. mouse. [Q9JLV2-1 ]

Organism-specific databases

CTDi 26133.
MGIi MGI:1930751. Trpc4ap.

Phylogenomic databases

eggNOGi NOG42180.
GeneTreei ENSGT00390000018330.
HOGENOMi HOG000231794.
HOVERGENi HBG059886.
InParanoidi Q9JLV2.
KOi K11796.
OMAi HYLNKDK.
OrthoDBi EOG7P02H9.
TreeFami TF329145.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_227861. TRP channels.

Miscellaneous databases

ChiTaRSi Trpc4ap. mouse.
NextBioi 312538.
PROi Q9JLV2.
SOURCEi Search...

Gene expression databases

Bgeei Q9JLV2.
ExpressionAtlasi Q9JLV2. baseline.
Genevestigatori Q9JLV2.

Family and domain databases

InterProi IPR016024. ARM-type_fold.
IPR022162. DUF3689.
[Graphical view ]
Pfami PF12463. DUF3689. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 4 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "TRUSS, a novel tumor necrosis factor receptor 1 scaffolding protein that mediates activation of the transcription factor NF-kappaB."
    Soond S.M., Terry J.L., Colbert J.D., Riches D.W.H.
    Mol. Cell. Biol. 23:8334-8344(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: BALB/c.
  2. Qian F., Philipson L.H.
    Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Insulinoma.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary gland.
  4. "A novel Rabex-5/Rin2-interacting protein."
    Scherrer D., Tsai M., Galli S.J., Tam S.-Y.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 154-797 (ISOFORM 1).
    Strain: BALB/c.
    Tissue: Brain.
  5. "TRUSS, a tumor necrosis factor receptor-1-interacting protein, activates c-Jun NH(2)-terminal kinase and transcription factor AP-1."
    Soond S.M., Terry J.L., Riches D.W.H.
    FEBS Lett. 580:4591-4596(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TRAF2.
  6. "TRUSS, TNF-R1, and TRPC ion channels synergistically reverse endoplasmic reticulum Ca2+ storage reduction in response to m1 muscarinic acetylcholine receptor signaling."
    Mace K.E., Lussier M.P., Boulay G., Terry-Powers J.L., Parfrey H., Perraud A.L., Riches D.W.H.
    J. Cell. Physiol. 225:444-453(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TRPC1; TRPC4 AND TRPC5.

Entry informationi

Entry nameiTP4AP_MOUSE
AccessioniPrimary (citable) accession number: Q9JLV2
Secondary accession number(s): Q920J6, Q99L03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 15, 2002
Last modified: November 26, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

External Data

Dasty 3