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Q9JLV2 (TP4AP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Short transient receptor potential channel 4-associated protein

Short name=Trp4-associated protein
Short name=Trpc4-associated protein
Alternative name(s):
Protein TAP1
Rabex-5/Rin2-interacting protein
TNF-receptor ubiquitous scaffolding/signaling protein
Short name=Protein TRUSS
Gene names
Name:Trpc4ap
Synonyms:Trrp4ap
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length797 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(TRUSS) complex specifically mediates the polyubiquitination and subsequent degradation of MYC By similarity. Also participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. Involved in JNK activation via its interaction with TRAF2. Also involved in elevation of endoplasmic reticulum Ca2+ storage reduction in response to CHRM1. Ref.5 Ref.6

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Component of the DCX(TRUSS) E3 ubiquitin ligase complex, at least composed of CUL4A, DDB1, TRPC4AP/TRUSS and RBX1. Interacts with MYC By similarity. Constitutively associated with TNFRSF1A. Directly interacts with TRADD, TRAF2, CHUK, IKBKB and IKBKG. Interacts with TRPC1, TRPC4 and TRPC5. Ref.5 Ref.6

Tissue specificity

Widely expressed, with high levels in heart, liver and testis.

Sequence caution

The sequence AAL08422.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9JLV2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9JLV2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     351-358: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 797796Short transient receptor potential channel 4-associated protein
PRO_0000072642

Regions

Region2 – 400399Interaction with TNFRSF1A

Amino acid modifications

Modified residue21N-acetylalanine By similarity

Natural variations

Alternative sequence351 – 3588Missing in isoform 2.
VSP_003983

Experimental info

Sequence conflict11 – 144GASR → LPYW in AAF36513. Ref.2
Sequence conflict1581I → L in AAF36513. Ref.2
Sequence conflict1931F → L in AAF36513. Ref.2
Sequence conflict3591G → S in AAM46938. Ref.1
Sequence conflict5321R → K in AAM46938. Ref.1
Sequence conflict7691L → V in AAL08422. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 15, 2002. Version 2.
Checksum: 8BC3453A7658A234

FASTA79790,727
        10         20         30         40         50         60 
MAAAPAAAGA GASRGRRLAA TAAAWGGWGG RPRPGNILLQ LRQGQLTGRG LVRAVQFTET 

        70         80         90        100        110        120 
FLTERDKLSK WSGIPQLLLK LYATSHLHSD FVECQSILKE ISPLLSMEAM AFVTEDRKFT 

       130        140        150        160        170        180 
QEATYPNTYI FDLFGGVDLL VEILMRPTIS IRGQKLKISD EMSKDCLSIL YNTCVCTEGV 

       190        200        210        220        230        240 
TKRLAEKNDF VIFLFTLMTS KKTFLQTATL IEDILGVKKE MIRLDEVPNL SSLVSNFDQQ 

       250        260        270        280        290        300 
QLANFCRILA VTISEMDTGN DDKHTLLAKN AQQKKSLSLG PSAAEINQAA LLSIPGFVER 

       310        320        330        340        350        360 
LCKLATRKVS ESTGTASFLQ ELEEWYTWLD NALVLDALMR VANEESEHNQ APTVFPSLGT 

       370        380        390        400        410        420 
SEEGGLPHTS ARAQLPQSMK IMHEIMYKLE VLYVLCVLLM GRQRNQVHRM IAEFKLIPGL 

       430        440        450        460        470        480 
NNLFDKLIWR KHSASALVLH GHNQNCDCSP DITLKIQFLR LLQSFSDHHE NKYLLLNNQE 

       490        500        510        520        530        540 
LNELSAISLK ANIPEVEAVL NTDRSLVCDG KRGLLTRLLQ VMKKEPAESS FRFWQARAVE 

       550        560        570        580        590        600 
SFLRGTTSYA DQMFLLKRGL LEHILYCIVD SECKSRDVLQ SYFDLLGELM KFNVDAFKRF 

       610        620        630        640        650        660 
NKYINTDAKF QVFLKQINSS LVDSNMLVRC VTLSLDRFEN QVDMKVAEVL SECRLLAYIS 

       670        680        690        700        710        720 
QVPTQMSFLF RLINIIHVQT LTQENVSCLN TSLVILMLAR RKERLPLYLR LLQRMEHSKK 

       730        740        750        760        770        780 
YPGFLLNNFH NLLRFWQQHY LHKDKDSTCL ENSSCISFSY WKETVSILLN PDRQSPSALV 

       790 
SYIEEPYMDI DRDFTEE 

« Hide

Isoform 2 [UniParc].

Checksum: 92D659B52F87206E
Show »

FASTA78989,914

References

« Hide 'large scale' references
[1]"TRUSS, a novel tumor necrosis factor receptor 1 scaffolding protein that mediates activation of the transcription factor NF-kappaB."
Soond S.M., Terry J.L., Colbert J.D., Riches D.W.H.
Mol. Cell. Biol. 23:8334-8344(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: BALB/c.
[2]Qian F., Philipson L.H.
Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Insulinoma.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Mammary gland.
[4]"A novel Rabex-5/Rin2-interacting protein."
Scherrer D., Tsai M., Galli S.J., Tam S.-Y.
Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 154-797 (ISOFORM 1).
Strain: BALB/c.
Tissue: Brain.
[5]"TRUSS, a tumor necrosis factor receptor-1-interacting protein, activates c-Jun NH(2)-terminal kinase and transcription factor AP-1."
Soond S.M., Terry J.L., Riches D.W.H.
FEBS Lett. 580:4591-4596(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TRAF2.
[6]"TRUSS, TNF-R1, and TRPC ion channels synergistically reverse endoplasmic reticulum Ca2+ storage reduction in response to m1 muscarinic acetylcholine receptor signaling."
Mace K.E., Lussier M.P., Boulay G., Terry-Powers J.L., Parfrey H., Perraud A.L., Riches D.W.H.
J. Cell. Physiol. 225:444-453(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TRPC1; TRPC4 AND TRPC5.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF509881 mRNA. Translation: AAM46938.1.
AF130458 mRNA. Translation: AAF36513.1.
BC003931 mRNA. Translation: AAH03931.1.
BC033274 mRNA. Translation: AAH33274.1.
BC057330 mRNA. Translation: AAH57330.1.
AF093589 mRNA. Translation: AAL08422.1. Different initiation.
RefSeqNP_001156924.1. NM_001163452.1.
NP_062802.2. NM_019828.2.
UniGeneMm.268304.
Mm.469980.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000037574.

PTM databases

PhosphoSiteQ9JLV2.

Proteomic databases

PaxDbQ9JLV2.
PRIDEQ9JLV2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000041059; ENSMUSP00000037574; ENSMUSG00000038324. [Q9JLV2-1]
ENSMUST00000103140; ENSMUSP00000099429; ENSMUSG00000038324. [Q9JLV2-2]
GeneID56407.
KEGGmmu:56407.
UCSCuc008nlc.2. mouse. [Q9JLV2-1]

Organism-specific databases

CTD26133.
MGIMGI:1930751. Trpc4ap.

Phylogenomic databases

eggNOGNOG42180.
GeneTreeENSGT00390000018330.
HOGENOMHOG000231794.
HOVERGENHBG059886.
InParanoidQ9JLV2.
KOK11796.
OMAQESTFPN.
OrthoDBEOG7P02H9.
TreeFamTF329145.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

BgeeQ9JLV2.
GenevestigatorQ9JLV2.

Family and domain databases

InterProIPR016024. ARM-type_fold.
IPR022162. DUF3689.
[Graphical view]
PfamPF12463. DUF3689. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 4 hits.
ProtoNetSearch...

Other

ChiTaRSTRPC4AP. mouse.
NextBio312538.
PROQ9JLV2.
SOURCESearch...

Entry information

Entry nameTP4AP_MOUSE
AccessionPrimary (citable) accession number: Q9JLV2
Secondary accession number(s): Q920J6, Q99L03
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 15, 2002
Last modified: April 16, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot