Q9JLV1 (BAG3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
BAG family molecular chaperone regulator 3
Short name=BAG-3

Alternative name(s):
Bcl-2-associated athanogene 3
Bcl-2-binding protein Bis

Gene names

Name:	Bag3
Synonyms:	Bis
ORF Names:	MNCb-2243

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
Subunit structure	Binds to the ATPase domain of HSP/HSC70 chaperones. Binds to Bcl-2 and PLC-gamma By similarity. Interacts with DNAJB6 By similarity.
Sequence similarities	Contains 1 BAG domain. Contains 2 WW domains.

Ontologies

Keywords
Biological process	Apoptosis
Domain	Repeat
Molecular function	Chaperone
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW brain development Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from direct assay Ref.1PubMed 11527400. Source: MGI spinal cord development Inferred from electronic annotation. Source: Compara
Cellular_component	Z disc Inferred from direct assay PubMed 16936253. Source: MGI cytosol Inferred from sequence orthology Ref.1. Source: MGI neuron projection Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 577

576

BAG family molecular chaperone regulator 3

PRO_0000088869

Regions

Domain

22 – 56

35

WW 1

Domain

126 – 157

32

WW 2

Domain

426 – 503

78

BAG

Compositional bias

186 – 193

8

Poly-Ser

Amino acid modifications

Modified residue

2

1

N-acetylserine By similarity

Modified residue

204

1

Phosphoserine By similarity

Modified residue

280

1

Phosphoserine By similarity

Modified residue

281

1

Phosphoserine By similarity

Modified residue

285

1

Phosphoserine By similarity

Modified residue

291

1

Phosphothreonine By similarity

Modified residue

297

1

Phosphoserine Ref.5

Modified residue

382

1

Phosphoserine By similarity

Modified residue

390

1

Phosphoserine Ref.6

Experimental info

Sequence conflict

74

1

D → N in AAF26840. Ref.1

Sequence conflict

527

1

Q → P in AAF26840. Ref.1

Sequence conflict

539

1

K → E in BAA95066. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

577

Helix Strand Turn

Details...

Beta strand

417 – 420

4

Helix

425 – 445

21

Beta strand

451 – 453

3

Helix

454 – 471

18

Helix

479 – 503

25

Sequences

	Sequence		Length	Mass (Da)
	Q9JLV1 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 7BBF296A4A2EF7E3	FASTA	577	61,860
	10 20 30 40 50 60 MSAATQSPMM QMASGNGASD RDPLPPGWEI KIDPQTGWPF FVDHNSRTTT WNDPRVPPEG 70 80 90 100 110 120 PKDTASSANG PSRDGSRLLP IREGHPIYPQ LRPGYIPIPV LHEGSENRQP HLFHAYSQPG 130 140 150 160 170 180 VQRFRTEAAA ATPQRSQSPL RGGMTEAAQT DKQCGQMPAT ATTAAAQPPT AHGPERSQSP 190 200 210 220 230 240 AASDCSSSSS SASLPSSGRS SLGSHQLPRG YIPIPVIHEQ NITRPAAQPS FHQAQKTHYP 250 260 270 280 290 300 AQQGEYQPQQ PVYHKIQGDD WEPRPLRAAS PFRSPVRGAS SREGSPARSG TPVHCPSPIR 310 320 330 340 350 360 VHTVVDRPQP MTHREPPPVT QPENKPESKP GPAGPDLPPG HIPIQVIRRE ADSKPVSQKS 370 380 390 400 410 420 PPPAEKVEVK VSSAPIPCPS PSPAPSAVPS PPKNVAAEQK AAPSPAPAEP AAPKSGEAET 430 440 450 460 470 480 PPKHPGVLKV EAILEKVQGL EQAVDSFEGK KTDKKYLMIE EYLTKELLAL DSVDPEGRAD 490 500 510 520 530 540 VRQARRDGVR KVQTILEKLE QKAIDVPGQV QVYELQPSNL EAEQPLQEIM GAVVADKDKK 550 560 570 GPENKDPQTE SQQLEAKAAT PPNPSNPADS AGNLVAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bis, a Bcl-2-binding protein that synergizes with Bcl-2 in preventing cell death." Lee J.H., Takahashi T., Yasuhara N., Inazawa J., Kamada S., Tsujimoto Y. Oncogene 18:6183-6190(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation of full-length cDNA clones from mouse brain cDNA library made by oligo-capping method." Osada N., Kusuda J., Tanuma R., Ito A., Hirata M., Sugano S., Hashimoto K. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Bone, Pancreas and Testis.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-297, MASS SPECTROMETRY. Tissue: Liver.
[6]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-390, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
[7]	"Solution structure of the murine BAG domain of Bcl2-associated athanogene 3." RIKEN structural genomics initiative (RSGI) Submitted (FEB-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 404-503.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF130471 mRNA. Translation: AAF26840.1.
AB041583 mRNA. Translation: BAA95066.1.
AK007414 mRNA. Translation: BAB25024.1.
AK016510 mRNA. Translation: BAB30278.1.
AK036675 mRNA. Translation: BAC29531.1.
CH466531 Genomic DNA. Translation: EDL17651.1.

IPI

IPI00331334.

RefSeq

NP_038891.4. NM_013863.5.

UniGene

Mm.84073.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UK5	NMR	-	A	406-503	[»]

ProteinModelPortal

Q9JLV1.

SMR

Q9JLV1. Positions 18-57, 401-503.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9JLV1. 1 interaction.

PTM databases

PhosphoSite

Q9JLV1.

Proteomic databases

PaxDb

Q9JLV1.

PRIDE

Q9JLV1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000033136; ENSMUSP00000033136; ENSMUSG00000030847.

GeneID

29810.

KEGG

mmu:29810.

Organism-specific databases

CTD

9531.

MGI

MGI:1352493. Bag3.

Phylogenomic databases

eggNOG

NOG238690.

GeneTree

ENSGT00530000063256.

HOGENOM

HOG000050234.

HOVERGEN

HBG003419.

InParanoid

Q9CQL3.

KO

K09557.

OMA

VYPQLRP.

OrthoDB

EOG4ZW5BW.

Gene expression databases

Bgee

Q9JLV1.

CleanEx

MM_BAG3.

Genevestigator

Q9JLV1.

GermOnline

ENSMUSG00000030847. Mus musculus.

Family and domain databases

InterPro

IPR003103. BAG_domain.
IPR001202. WW_dom.
[Graphical view]

Pfam

PF02179. BAG. 1 hit.
PF00397. WW. 1 hit.
[Graphical view]

SMART

SM00264. BAG. 1 hit.
SM00456. WW. 1 hit.
[Graphical view]

SUPFAM

SSF51045. WW_Rsp5_WWP. 1 hit.

PROSITE

PS51035. BAG. 1 hit.
PS01159. WW_DOMAIN_1. 1 hit.
PS50020. WW_DOMAIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9JLV1.

NextBio

306958.

SOURCE

Search...

Entry information

Entry name

BAG3_MOUSE

Accession

Primary (citable) accession number: Q9JLV1
Secondary accession number(s): Q9CQL3, Q9JJC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program