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Reviewed, UniProtKB/Swiss-Prot Q9JLS4 (SFRP4_RAT)

Last modified January 19, 2010. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Secreted frizzled-related protein 4
      Short name=sFRP-4
      Short name=Frizzled-related protein
      Short name=FRP
Alternative name(s):
    DDC-4 protein
Gene names
Name: Sfrp4
Synonyms: Ddc4, Frp
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake By similarity.

Subcellular location

Secreted By similarity.

Tissue specificity

Expressed in the involuting mammary gland, ovarian corpus luteum and prostate. In ovaries, low levels found in granulosa cells. High levels in corpora lutea of pregnant animals. Ref.5

Developmental stage

Expressed from day 9 of pregnant uterus. Highest level at day 12, specifically in the decidua and weakly, in the myometrium. Levels decline thereafter. Ref.4

Induction

Up-regulated 48 hours after estrogen treatment mainly in the uterine endometrial stroma. Induced in ovarian granulosa cells after 12 hours treatment with chorionic gonadotrophin (CG). Further up-regulated in corpora lutea by the luteotrophic hormone PRL. Ref.5

Domain

The FZ domain is involved in binding with Wnt ligands By similarity.

Sequence similarities

Belongs to the secreted frizzled-related protein (sFRP) family.

Contains 1 FZ (frizzled) domain.

Contains 1 NTR domain.

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Ontologies

Keywords
   Biological processDifferentiation
Wnt signaling pathway
   Cellular componentSecreted
   DomainSignal
   Molecular functionDevelopmental protein
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Biological processWnt receptor signaling pathway Ref.4

Traceable author statement. Source: RGD

cell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

multicellular organismal development

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionprotein binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 348330Secreted frizzled-related protein 4
PRO_0000032553

Regions

Domain19 – 139121FZ
Domain178 – 296119NTR

Amino acid modifications

Glycosylation381N-linked (GlcNAc...) Potential
Glycosylation681N-linked (GlcNAc...) Potential
Glycosylation1161N-linked (GlcNAc...) Potential
Glycosylation1941N-linked (GlcNAc...) Potential
Glycosylation2401N-linked (GlcNAc...) Potential
Disulfide bond24 ↔ 85 By similarity
Disulfide bond32 ↔ 78 By similarity
Disulfide bond69 ↔ 108 By similarity
Disulfide bond97 ↔ 136 By similarity
Disulfide bond101 ↔ 125 By similarity

Experimental info

Sequence conflict12 – 132CV → WL in AAF66480. Ref.2
Sequence conflict12 – 132CV → WL in AAF66481. Ref.2
Sequence conflict591E → G in AAF66480. Ref.2
Sequence conflict741R → S in AAF66480. Ref.2
Sequence conflict741R → S in AAF66481. Ref.2
Sequence conflict1011C → S in AAF66481. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Q9JLS4-1 [UniParc].

Last modified March 15, 2005. Version 2.
Checksum: 08A0F0E3B80561CF

FASTA34839,762
        10         20         30         40         50         60 
MLLSILVALC LCVRLALGVR GAPCEAVRIP MCRHMPWNIT RMPNHLHHST QENAILAIEQ 

        70         80         90        100        110        120 
YEELVDVNCS SVLRFFLCAM YAPICTLEFL HDPIKPCKSV CQRARDDCEP LMKMYNHSWP 

       130        140        150        160        170        180 
ESLACDELPV YDRGVCISPE AIVTDLPEDV KWIDITPDMM VQERSFDADC KHLSPDRCKC 

       190        200        210        220        230        240 
KKVKPTLATY LSKNYSYVIH AKIKAVQRSG CNEVTTVVDV KEIFKSSSPI PRTQVPLITN 

       250        260        270        280        290        300 
SSCQCPHILP HQDVLIMCYE RRSRMMLLEN CLVEKWRDQL SRRSTQWEER LQEQQRTTQD 

       310        320        330        340 
KKQIASRTSR SNPPKPKGRS PASKPASPKK NIKARSAPKK SNPKKSTS

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References

[1]"DDC-4, an apoptosis-associated gene, is a secreted frizzled relative."
Wolf V., Ke G., Dharmarajan A.M., Bielke W., Artuso L., Saurer S., Friis R.R.
FEBS Lett. 417:385-389(1997) [PubMed: 9409757] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Corpus luteum.
[2]"Transcriptional activity of the promoter region of rat frizzled-related protein gene."
Yam J.W.P., Chan K.W., Wong V.K.W., Hsiao W.L.W.
Biochem. Biophys. Res. Commun. 286:94-100(2001) [PubMed: 11485313] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: Fischer.
Tissue: Fibroblast and Liver.
[3]"Apoptosis-associated gene expression in the corpus luteum of the rat."
Guo K., Wolf V., Dharmarajan A.M., Feng Z., Bielke W., Saurer S., Friis R.
Biol. Reprod. 58:739-746(1998) [PubMed: 9510961] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-140.
Strain: Sprague-Dawley.
[4]"Differential expression of secreted frizzled-related protein 4 in decidual cells during pregnancy."
Fujita M., Ogawa S., Fukuoka H., Tsukui T., Nemoto N., Tsutsumi O., Ouchi Y., Inoue S.
J. Mol. Endocrinol. 28:213-223(2002) [PubMed: 12063187] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 190-347, DEVELOPMENTAL STAGE.
Strain: Wistar.
Tissue: Uterus.
[5]"Expression and localization of secreted frizzled-related protein-4 in the rodent ovary: evidence for selective up-regulation in luteinized granulosa cells."
Hsieh M., Mulders S.M., Friis R.R., Dharmarajan A., Richards J.S.
Endocrinology 144:4597-4606(2003) [PubMed: 12960062] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF012891 mRNA. Translation: AAB65431.1.
AF140346 mRNA. Translation: AAF66480.1.
AF140347 Genomic DNA. Translation: AAF66481.1.
IPIIPI00207235.
PIRJC7735.
RefSeqNP_445996.1.
UniGeneRn.10788

3D structure databases

SMRQ9JLS4. Positions 22-144, 179-283.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9JLS4.

Genome annotation databases

EnsemblENSRNOT00000025645; ENSRNOP00000025645; ENSRNOG00000018893; Rattus norvegicus. [Genome view]
GeneID89803.
KEGGrno:89803.
UCSCNM_053544. rat.

Organism-specific databases

CTD89803.
RGD621075. Sfrp4.

Phylogenomic databases

eggNOGroNOG14236.
HOVERGENQ9JLS4.
InParanoidQ9JLS4.

Gene expression databases

ArrayExpressQ9JLS4.
GenevestigatorQ9JLS4.
GermOnlineENSRNOG00000018893. Rattus norvegicus.

Family and domain databases

InterProIPR020067. Frizzled-like_dom.
IPR000024. Frizzled_Cys-rich.
IPR020068. Frizzled_dom_subgr.
IPR015526. Frizzled_related.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
Gene3DG3DSA:1.10.2000.10. Frizzled_Cys-rich. 1 hit.
PANTHERPTHR11309. Fz_related. 1 hit.
PfamPF01392. Fz. 1 hit.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTSM00643. C345C. 1 hit.
SM00063. FRI. 1 hit.
[Graphical view]
PROSITEPS50038. FZ. 1 hit.
PS50189. NTR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio617638.

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Entry information

Entry nameSFRP4_RAT
AccessionPrimary (citable) accession number: Q9JLS4
Secondary accession number(s): O35222, Q9JLS5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: January 19, 2010
This is version 55 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents