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Protein

Secreted frizzled-related protein 4

Gene

Sfrp4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types (By similarity). SFRP4 plays a role in bone morphogenesis (By similarity). May also act as a regulator of adult uterine morphology and function (PubMed:12063187). May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (PubMed:12960062). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake (By similarity).2 PublicationsBy similarity

GO - Molecular functioni

GO - Biological processi

  • bone morphogenesis Source: UniProtKB
  • cell differentiation Source: UniProtKB-KW
  • decidualization Source: RGD
  • female pregnancy Source: RGD
  • mammary gland involution Source: RGD
  • negative regulation of canonical Wnt signaling pathway Source: BHF-UCL
  • negative regulation of cell proliferation Source: RGD
  • negative regulation of JNK cascade Source: RGD
  • negative regulation of non-canonical Wnt signaling pathway Source: UniProtKB
  • non-canonical Wnt signaling pathway Source: GO_Central
  • regulation of BMP signaling pathway Source: UniProtKB
  • response to estradiol Source: RGD
  • response to glucocorticoid Source: RGD
  • response to peptide hormone Source: RGD
  • Wnt signaling pathway Source: RGD

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Secreted frizzled-related protein 4
Short name:
sFRP-4
Alternative name(s):
DDC-4 protein
Gene namesi
Name:Sfrp4
Synonyms:Ddc4, Frp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621075. Sfrp4.

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

  • cytoplasm Source: RGD
  • extracellular space Source: RGD
  • integral component of membrane Source: GO_Central

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000003255319 – 348Secreted frizzled-related protein 4Add BLAST330

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi24 ↔ 85By similarity
Disulfide bondi32 ↔ 78By similarity
Glycosylationi38N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi68N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi69 ↔ 108By similarity
Disulfide bondi97 ↔ 136By similarity
Disulfide bondi101 ↔ 125By similarity
Glycosylationi116N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi194N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi240N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9JLS4.

Expressioni

Tissue specificityi

Expressed in the involuting mammary gland, ovarian corpus luteum and prostate. In ovaries, low levels found in granulosa cells. High levels in corpora lutea of pregnant animals.1 Publication

Developmental stagei

Expressed from day 9 of pregnant uterus. Highest level at day 12, specifically in the decidua and weakly, in the myometrium. Levels decline thereafter.1 Publication

Inductioni

Up-regulated 48 hours after estrogen treatment mainly in the uterine endometrial stroma (PubMed:12063187). Induced in ovarian granulosa cells after 12 hours treatment with chorionic gonadotrophin (CG). Further up-regulated in corpora lutea by the luteotrophic hormone PRL (PubMed:12960062).2 Publications

Interactioni

GO - Molecular functioni

  • Wnt-protein binding Source: RGD

Protein-protein interaction databases

IntActiQ9JLS4. 1 interactor.
STRINGi10116.ENSRNOP00000025645.

Structurei

3D structure databases

ProteinModelPortaliQ9JLS4.
SMRiQ9JLS4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 139FZPROSITE-ProRule annotationAdd BLAST121
Domaini178 – 296NTRPROSITE-ProRule annotationAdd BLAST119

Domaini

The FZ domain is involved in binding with Wnt ligands.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3577. Eukaryota.
ENOG410XRC8. LUCA.
HOGENOMiHOG000231879.
HOVERGENiHBG070536.
InParanoidiQ9JLS4.
KOiK02185.
PhylomeDBiQ9JLS4.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiView protein in InterPro
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR026560. SFRP4.
IPR008993. TIMP-like_OB-fold.
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF99. PTHR11309:SF99. 1 hit.
PfamiView protein in Pfam
PF01392. Fz. 1 hit.
PF01759. NTR. 1 hit.
SMARTiView protein in SMART
SM00643. C345C. 1 hit.
SM00063. FRI. 1 hit.
SUPFAMiSSF50242. SSF50242. 1 hit.
SSF63501. SSF63501. 1 hit.
PROSITEiView protein in PROSITE
PS50038. FZ. 1 hit.
PS50189. NTR. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JLS4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLSILVALC LCVRLALGVR GAPCEAVRIP MCRHMPWNIT RMPNHLHHST
60 70 80 90 100
QENAILAIEQ YEELVDVNCS SVLRFFLCAM YAPICTLEFL HDPIKPCKSV
110 120 130 140 150
CQRARDDCEP LMKMYNHSWP ESLACDELPV YDRGVCISPE AIVTDLPEDV
160 170 180 190 200
KWIDITPDMM VQERSFDADC KHLSPDRCKC KKVKPTLATY LSKNYSYVIH
210 220 230 240 250
AKIKAVQRSG CNEVTTVVDV KEIFKSSSPI PRTQVPLITN SSCQCPHILP
260 270 280 290 300
HQDVLIMCYE RRSRMMLLEN CLVEKWRDQL SRRSTQWEER LQEQQRTTQD
310 320 330 340
KKQIASRTSR SNPPKPKGRS PASKPASPKK NIKARSAPKK SNPKKSTS
Length:348
Mass (Da):39,762
Last modified:March 15, 2005 - v2
Checksum:i08A0F0E3B80561CF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12 – 13CV → WL in AAF66480 (PubMed:11485313).Curated2
Sequence conflicti12 – 13CV → WL in AAF66481 (PubMed:11485313).Curated2
Sequence conflicti59E → G in AAF66480 (PubMed:11485313).Curated1
Sequence conflicti74R → S in AAF66480 (PubMed:11485313).Curated1
Sequence conflicti74R → S in AAF66481 (PubMed:11485313).Curated1
Sequence conflicti101C → S in AAF66481 (PubMed:11485313).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012891 mRNA. Translation: AAB65431.1.
AF140346 mRNA. Translation: AAF66480.1.
AF140347 Genomic DNA. Translation: AAF66481.1.
PIRiJC7735.
RefSeqiNP_445996.1. NM_053544.1.
UniGeneiRn.10788.

Genome annotation databases

GeneIDi89803.
KEGGirno:89803.
UCSCiRGD:621075. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSFRP4_RAT
AccessioniPrimary (citable) accession number: Q9JLS4
Secondary accession number(s): O35222, Q9JLS5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: July 5, 2017
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families