Sfrp4

Functionⁱ

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types (By similarity). SFRP4 plays a role in bone morphogenesis (By similarity). May also act as a regulator of adult uterine morphology and function (PubMed:12063187). May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (PubMed:12960062). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake (By similarity).2 PublicationsBy similarity

GO - Molecular functionⁱ

G-protein coupled receptor activity Source: GO_Central
Wnt-activated receptor activity Source: GO_Central

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

bone morphogenesis Source: UniProtKB
cell differentiation Source: UniProtKB-KW
decidualization
Source: RGD
Inferred from expression patternⁱ
- 12063187
female pregnancy
Source: RGD
Inferred from expression patternⁱ
- 16540541
mammary gland involution
Source: RGD
Inferred from expression patternⁱ
- 9409757
negative regulation of canonical Wnt signaling pathway
Source: BHF-UCL
Inferred from direct assayⁱ
- 12952927
negative regulation of cell proliferation
Source: RGD
Inferred from direct assayⁱ
- 18567805
negative regulation of JNK cascade
Source: RGD
Inferred from direct assayⁱ
- 18567805
negative regulation of non-canonical Wnt signaling pathway Source: UniProtKB
non-canonical Wnt signaling pathway Source: GO_Central
regulation of BMP signaling pathway Source: UniProtKB
response to estradiol
Source: RGD
Inferred from expression patternⁱ
- 12063187
response to glucocorticoid
Source: RGD
Inferred from expression patternⁱ
- 16540541
response to peptide hormone
Source: RGD
Inferred from expression patternⁱ
- 12960062
Wnt signaling pathway
Source: RGD
Traceable author statementⁱ
- 12063187

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Developmental protein
Biological process	Differentiation, Wnt signaling pathway

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Secreted frizzled-related protein 4 Short name: sFRP-4 Alternative name(s): DDC-4 protein
Gene namesⁱ	Name:Sfrp4 Synonyms:Ddc4, Frp
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Unplaced

Organism-specific databases

Rat genome database More...RGDⁱ	621075 Sfrp4

RGDⁱ

621075 Sfrp4

Keywords - Cellular componentⁱ

Secreted

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 18	Sequence analysisAdd BLAST		18
ChainⁱPRO_0000032553	19 – 348	Secreted frizzled-related protein 4Add BLAST		330

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	24 ↔ 85	By similarity
Disulfide bondⁱ	32 ↔ 78	By similarity
Glycosylationⁱ	38	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	68	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	69 ↔ 108	By similarity
Disulfide bondⁱ	97 ↔ 136	By similarity
Disulfide bondⁱ	101 ↔ 125	By similarity
Glycosylationⁱ	116	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	194	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	240	N-linked (GlcNAc...) asparagineSequence analysis	1

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbⁱ	Q9JLS4
PRIDEⁱ	Q9JLS4

PTM databases

CarbonylDBⁱ	Q9JLS4

CarbonylDBⁱ

Q9JLS4

Expressionⁱ

Tissue specificityⁱ

Expressed in the involuting mammary gland, ovarian corpus luteum and prostate. In ovaries, low levels found in granulosa cells. High levels in corpora lutea of pregnant animals.1 Publication

Developmental stageⁱ

Expressed from day 9 of pregnant uterus. Highest level at day 12, specifically in the decidua and weakly, in the myometrium. Levels decline thereafter.1 Publication

Inductionⁱ

Up-regulated 48 hours after estrogen treatment mainly in the uterine endometrial stroma (PubMed:12063187). Induced in ovarian granulosa cells after 12 hours treatment with chorionic gonadotrophin (CG). Further up-regulated in corpora lutea by the luteotrophic hormone PRL (PubMed:12960062).2 Publications

Interactionⁱ

Protein-protein interaction databases

IntActⁱ	Q9JLS4, 1 interactor
STRINGⁱ	10116.ENSRNOP00000025645

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9JLS4
SMRⁱ	Q9JLS4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	19 – 139	FZPROSITE-ProRule annotationAdd BLAST		121
Domainⁱ	178 – 296	NTRPROSITE-ProRule annotationAdd BLAST		119

Domainⁱ

The FZ domain is involved in binding with Wnt ligands.By similarity

Sequence similaritiesⁱ

Belongs to the secreted frizzled-related protein (sFRP) family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	KOG3577 Eukaryota ENOG410XRC8 LUCA
HOGENOMⁱ	HOG000231879
HOVERGENⁱ	HBG070536
InParanoidⁱ	Q9JLS4
KEGG Orthology (KO) More...KOⁱ	K02185
PhylomeDBⁱ	Q9JLS4

Family and domain databases

Gene3Dⁱ	1.10.2000.10, 1 hit
InterProⁱ	View protein in InterPro IPR015526 Frizzled/SFRP IPR020067 Frizzled_dom IPR036790 Frizzled_dom_sf IPR001134 Netrin_domain IPR018933 Netrin_module_non-TIMP IPR026560 SFRP4 IPR008993 TIMP-like_OB-fold
PANTHERⁱ	PTHR11309 PTHR11309, 1 hit PTHR11309:SF7 PTHR11309:SF7, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01392 Fz, 1 hit PF01759 NTR, 1 hit
SMARTⁱ	View protein in SMART SM00643 C345C, 1 hit SM00063 FRI, 1 hit
SUPFAMⁱ	SSF50242 SSF50242, 1 hit SSF63501 SSF63501, 1 hit
PROSITEⁱ	View protein in PROSITE PS50038 FZ, 1 hit PS50189 NTR, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q9JLS4-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLLSILVALC LCVRLALGVR GAPCEAVRIP MCRHMPWNIT RMPNHLHHST 
        60         70         80         90        100
QENAILAIEQ YEELVDVNCS SVLRFFLCAM YAPICTLEFL HDPIKPCKSV 
       110        120        130        140        150
CQRARDDCEP LMKMYNHSWP ESLACDELPV YDRGVCISPE AIVTDLPEDV 
       160        170        180        190        200
KWIDITPDMM VQERSFDADC KHLSPDRCKC KKVKPTLATY LSKNYSYVIH 
       210        220        230        240        250
AKIKAVQRSG CNEVTTVVDV KEIFKSSSPI PRTQVPLITN SSCQCPHILP 
       260        270        280        290        300
HQDVLIMCYE RRSRMMLLEN CLVEKWRDQL SRRSTQWEER LQEQQRTTQD 
       310        320        330        340 
KKQIASRTSR SNPPKPKGRS PASKPASPKK NIKARSAPKK SNPKKSTS

Length:348

Mass (Da):39,762

Last modified:March 15, 2005 - v2

Checksum:ⁱ08A0F0E3B80561CF

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	12 – 13	CV → WL in AAF66480 (PubMed:11485313).Curated	2
Sequence conflictⁱ	12 – 13	CV → WL in AAF66481 (PubMed:11485313).Curated	2
Sequence conflictⁱ	59	E → G in AAF66480 (PubMed:11485313).Curated	1
Sequence conflictⁱ	74	R → S in AAF66480 (PubMed:11485313).Curated	1
Sequence conflictⁱ	74	R → S in AAF66481 (PubMed:11485313).Curated	1
Sequence conflictⁱ	101	C → S in AAF66481 (PubMed:11485313).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF012891 mRNA Translation: AAB65431.1 AF140346 mRNA Translation: AAF66480.1 AF140347 Genomic DNA Translation: AAF66481.1
PIRⁱ	JC7735
NCBI Reference Sequences More...RefSeqⁱ	NP_445996.1, NM_053544.1
UniGeneⁱ	Rn.10788

Genome annotation databases

GeneIDⁱ	89803
KEGGⁱ	rno:89803
UCSC genome browser More...UCSCⁱ	RGD:621075 rat

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
Q9JLS4	Secreted frizzled-related sequence protein 4		MOUSE		351
UPI0002233997		LOXAF		408
UPI00045E1BBF		Orycteropus afer afer		360
UPI0003F18C67		CHRAS		354
UPI0002C424D4		Florida manatee		353
+29

Protein	Similar proteins		Species	Score	Length
Q9JLS4	Secreted frizzled-related sequence protein 4		MOUSE		351
UPI0002233997		LOXAF		408
UPI00045E1BBF		Orycteropus afer afer		360
UPI0003F18C67		CHRAS		354
UPI0003F05821		ELEED		353
+36

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF012891 mRNA Translation: AAB65431.1 AF140346 mRNA Translation: AAF66480.1 AF140347 Genomic DNA Translation: AAF66481.1
PIRⁱ	JC7735
NCBI Reference Sequences More...RefSeqⁱ	NP_445996.1, NM_053544.1
UniGeneⁱ	Rn.10788

3D structure databases

ProteinModelPortalⁱ	Q9JLS4
SMRⁱ	Q9JLS4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	Q9JLS4, 1 interactor
STRINGⁱ	10116.ENSRNOP00000025645

PTM databases

CarbonylDBⁱ	Q9JLS4

CarbonylDBⁱ

Q9JLS4

Proteomic databases

PaxDbⁱ	Q9JLS4
PRIDEⁱ	Q9JLS4

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	89803
KEGGⁱ	rno:89803
UCSC genome browser More...UCSCⁱ	RGD:621075 rat

Organism-specific databases

CTDⁱ	6424
Rat genome database More...RGDⁱ	621075 Sfrp4

Phylogenomic databases

eggNOGⁱ	KOG3577 Eukaryota ENOG410XRC8 LUCA
HOGENOMⁱ	HOG000231879
HOVERGENⁱ	HBG070536
InParanoidⁱ	Q9JLS4
KEGG Orthology (KO) More...KOⁱ	K02185
PhylomeDBⁱ	Q9JLS4

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9JLS4

PROⁱ

PR:Q9JLS4

Family and domain databases

Gene3Dⁱ	1.10.2000.10, 1 hit
InterProⁱ	View protein in InterPro IPR015526 Frizzled/SFRP IPR020067 Frizzled_dom IPR036790 Frizzled_dom_sf IPR001134 Netrin_domain IPR018933 Netrin_module_non-TIMP IPR026560 SFRP4 IPR008993 TIMP-like_OB-fold
PANTHERⁱ	PTHR11309 PTHR11309, 1 hit PTHR11309:SF7 PTHR11309:SF7, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01392 Fz, 1 hit PF01759 NTR, 1 hit
SMARTⁱ	View protein in SMART SM00643 C345C, 1 hit SM00063 FRI, 1 hit
SUPFAMⁱ	SSF50242 SSF50242, 1 hit SSF63501 SSF63501, 1 hit
PROSITEⁱ	View protein in PROSITE PS50038 FZ, 1 hit PS50189 NTR, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SFRP4_RAT
Accessionⁱ	Q9JLS4Primary (citable) accession number: Q9JLS4 Secondary accession number(s): O35222, Q9JLS5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
	Last sequence update:	March 15, 2005
	Last modified:	May 23, 2018
	This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

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UniProtKB - Q9JLS4 (SFRP4_RAT)

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Secreted frizzled-related protein 4

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

Extracellular region or secreted

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ