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Protein

Serine/threonine-protein kinase TAO2

Gene

Taok2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase involved in different processes such as membrane blebbing and apoptotic bodies formation DNA damage response and MAPK14/p38 MAPK stress-activated MAPK cascade. Phosphorylates itself, MBP, activated MAPK8, MAP2K3, MAP2K6 and tubulins. Activates the MAPK14/p38 MAPK signaling pathway through the specific activation and phosphorylation of the upstream MAP2K3 and MAP2K6 kinases. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. May affect microtubule organization and stability. May play a role in the osmotic stress-MAPK8 pathway. Prevents MAP3K7-mediated activation of CHUK, and thus NF-kappa-B activation. Isoform 2, but not isoform 1, is required for PCDH8 endocytosis. Following homophilic interactions between PCDH8 extracellular domains, isoform 2 phosphorylates and activates MAPK14/p38 MAPK which in turn phosphorylates isoform 2. This process leads to PCDH8 endocytosis and CDH2 cointernalization. Both isoforms are involved in MAPK14/p38 MAPK activation.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Enzyme regulationi

Moderately inhibited by staurosporine, a broad-range protein kinase inhibitor.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei57ATPPROSITE-ProRule annotation1
Binding sitei106Staurosporine inhibitor1 Publication1
Binding sitei108Staurosporine inhibitor1 Publication1
Active sitei151Proton acceptorPROSITE-ProRule annotation1
Binding sitei155Staurosporine inhibitor; via carbonyl oxygen1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi34 – 42ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: RGD
  • MAP kinase kinase kinase activity Source: RGD
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase TAO2 (EC:2.7.11.1)
Alternative name(s):
Thousand and one amino acid protein 2
Gene namesi
Name:Taok2
Synonyms:Tao2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621590. Taok2.

Subcellular locationi

Isoform 2 :

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei967 – 987HelicalSequence analysisAdd BLAST21
Transmembranei989 – 1009HelicalSequence analysisAdd BLAST21
Transmembranei1014 – 1034HelicalSequence analysisAdd BLAST21
Transmembranei1040 – 1060HelicalSequence analysisAdd BLAST21
Transmembranei1170 – 1190HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi57K → A: Loss of activity. Loss of MAPK14 phosphorylation and of PCDH8 internalization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867351 – 1235Serine/threonine-protein kinase TAO2Add BLAST1235

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineBy similarity1
Modified residuei181Phosphoserine1 Publication1
Modified residuei416PhosphoserineBy similarity1
Modified residuei658PhosphoserineBy similarity1
Modified residuei777PhosphoserineBy similarity1
Modified residuei825PhosphoserineBy similarity1
Modified residuei827PhosphoserineBy similarity1
Isoform 2 (identifier: Q9JLS3-2)
Modified residuei1038Phosphoserine1 Publication1

Post-translational modificationi

Autophosphorylated. Phosphorylated by ATM (By similarity).By similarity
Isoform 2: Phosphorylated on Ser-1038 by MAPK14. This phosphorylation is required PCDH8 for endocytosis.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9JLS3.
PRIDEiQ9JLS3.

PTM databases

iPTMnetiQ9JLS3.
PhosphoSitePlusiQ9JLS3.

Interactioni

Subunit structurei

Self-associates. Interacts with MAP2K3 and MAP2K6. Interacts with tubulins. Interacts with MAP3K7 and interfers with MAP3K7-binding to CHUK and thus prevents NF-kappa-B activation (By similarity). Isoform 2 interacts with PCDH8; this complex may also include CDH2.By similarity3 Publications

Protein-protein interaction databases

IntActiQ9JLS3. 1 interactor.
STRINGi10116.ENSRNOP00000061854.

Structurei

Secondary structure

11235
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 18Combined sources6
Helixi24 – 27Combined sources4
Beta strandi28 – 36Combined sources9
Beta strandi38 – 47Combined sources10
Turni48 – 51Combined sources4
Beta strandi52 – 61Combined sources10
Helixi66 – 82Combined sources17
Beta strandi91 – 97Combined sources7
Beta strandi100 – 106Combined sources7
Beta strandi109 – 111Combined sources3
Helixi112 – 119Combined sources8
Helixi125 – 144Combined sources20
Helixi154 – 156Combined sources3
Beta strandi157 – 160Combined sources4
Turni161 – 163Combined sources3
Beta strandi164 – 167Combined sources4
Beta strandi174 – 179Combined sources6
Helixi186 – 188Combined sources3
Helixi191 – 195Combined sources5
Turni196 – 199Combined sources4
Helixi205 – 220Combined sources16
Turni224 – 227Combined sources4
Helixi230 – 239Combined sources10
Helixi252 – 261Combined sources10
Helixi266 – 268Combined sources3
Helixi272 – 275Combined sources4
Helixi279 – 282Combined sources4
Helixi289 – 303Combined sources15
Beta strandi304 – 306Combined sources3
Helixi307 – 309Combined sources3
Helixi311 – 319Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U5QX-ray2.10A/B1-320[»]
1U5RX-ray2.10A/B1-320[»]
2GCDX-ray2.55A/B12-320[»]
ProteinModelPortaliQ9JLS3.
SMRiQ9JLS3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JLS3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 281Protein kinasePROSITE-ProRule annotationAdd BLAST254

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili488 – 523Sequence analysisAdd BLAST36
Coiled coili576 – 603Sequence analysisAdd BLAST28
Coiled coili683 – 715Sequence analysisAdd BLAST33

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi330 – 336Poly-Glu7
Compositional biasi347 – 370Ser-richAdd BLAST24
Compositional biasi378 – 408Glu-richAdd BLAST31
Compositional biasi450 – 455Poly-Ser6
Compositional biasi791 – 794Poly-Glu4
Compositional biasi900 – 908Poly-Glu9
Compositional biasi945 – 1062Leu-richAdd BLAST118

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0577. Eukaryota.
ENOG410Y259. LUCA.
HOGENOMiHOG000236358.
HOVERGENiHBG088996.
InParanoidiQ9JLS3.
KOiK04429.
PhylomeDBiQ9JLS3.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JLS3-1) [UniParc]FASTAAdd to basket
Also known as: TAO2-alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPAGGRAGSL KDPDVAELFF KDDPEKLFSD LREIGHGSFG AVYFARDVRN
60 70 80 90 100
SEVVAIKKMS YSGKQSNEKW QDIIKEVRFL QKLRHPNTIQ YRGCYLREHT
110 120 130 140 150
AWLVMEYCLG SASDLLEVHK KPLQEVEIAA VTHGALQGLA YLHSHNMIHR
160 170 180 190 200
DVKAGNILLS EPGLVKLGDF GSASIMAPAN SFVGTPYWMA PEVILAMDEG
210 220 230 240 250
QYDGKVDVWS LGITCIELAE RKPPLFNMNA MSALYHIAQN ESPALQSGHW
260 270 280 290 300
SEYFRNFVDS CLQKIPQDRP TSEVLLKHRF VLRERPPTVI MDLIQRTKDA
310 320 330 340 350
VRELDNLQYR KMKKILFQEA PNGPGAEAPE EEEEAEPYMH RAGTLTSLES
360 370 380 390 400
SHSVPSMSIS ASSQSSSVNS LADASDNEEE EEEEEEEEEE EEEEGPESRE
410 420 430 440 450
MAMMQEGEHT VTSHSSIIHR LPGSDNLYDD PYQPEMTPGP LQPPAAPPTS
460 470 480 490 500
TSSSSARRRA YCRNRDHFAT IRTASLVSRQ IQEHEQDSAL REQLSGYKRM
510 520 530 540 550
RRQHQKQLLA LESRLRGERE EHSGRLQREL EAQRAGFGTE AEKLARRHQA
560 570 580 590 600
IGEKEARAAQ AEERKFQQHI LGQQKKELAA LLEAQKRTYK LRKEQLKEEL
610 620 630 640 650
QENPSTPKRE KAEWLLRQKE QLQQCQAEEE AGLLRRQRQY FELQCRQYKR
660 670 680 690 700
KMLLARHSLD QDLLREDLNK KQTQKDLECA LLLRQHEATR ELELRQLQAV
710 720 730 740 750
QRTRAELTRL QHQTELGNQL EYNKRREQEL RQKHAAQVRQ QPKSLKVRAG
760 770 780 790 800
QLPMGLPATG ALGPLSTGTL SEEQPCSSGQ EAILGQRMLG EEEEAVPERM
810 820 830 840 850
ILGKEGTTLE PEEQRILGEE MGTFSSSPQK HRSLVNEEDW DISKEMKESR
860 870 880 890 900
VPSLASQERN IIGQEEAGAW NLWEKEHGNL VDMEFKLGWV QGPVLTPVPE
910 920 930 940 950
EEEEEEEEGG APIGTPRDPG DGCPSPDIPP EPPPSHLRQY PASQLPGFLS
960 970 980 990 1000
HGLLTGLSFA VGSSSGLLPL LLLLLLPLLA AQGGGGLQAA LLALEVGLVG
1010 1020 1030 1040 1050
LGASYLFLCT ALHLPPSLFL LLAQGTALGA VLSLSWRRGL MGVPLGLGAA
1060 1070 1080 1090 1100
WLLAWPSLAL PLAAMAAGGK WVRQQGPQMR RGISRLWLRV LLRLSPMVFR
1110 1120 1130 1140 1150
ALQGCAAVGD RGLFALYPKT NKNGFRSRLP VPWPRQGNPR TTQHPLALLA
1160 1170 1180 1190 1200
RVWALCKGWN WRLARASHRL ASCLPPWAVH ILASWGLLKG ERPSRIPRLL
1210 1220 1230
PRSQRRLGLS ASRQLPPGTV AGRRSQTRRA LPPWR
Length:1,235
Mass (Da):138,751
Last modified:October 1, 2000 - v1
Checksum:i426960D0812518AD
GO
Isoform 2 (identifier: Q9JLS3-2) [UniParc]FASTAAdd to basket
Also known as: TAO2-beta

The sequence of this isoform differs from the canonical sequence as follows:
     333-333: E → ELTPCSQ
     747-1050: VRAGQLPMGL...MGVPLGLGAA → SKELQIKKQF...ILNGSSHFYS
     1051-1235: Missing.

Show »
Length:1,056
Mass (Da):119,965
Checksum:iE6BCAD23F13A189A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti434P → L in BAF64457 (PubMed:17988630).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040545333E → ELTPCSQ in isoform 2. 1 Publication1
Alternative sequenceiVSP_040546747 – 1050VRAGQ…GLGAA → SKELQIKKQFQETCKIQTRQ YKALRAHLLETTPKAQHKSL VKRLKEEQTRKLAILAEQYD QSISEMLSSQALRLDETQEA EFQALRQRSNRNWSSLMLTR ARSKIRTESQHERELRELEQ RVALRRALLEQRVEEELLAL QTGRSERIRSLLERQAREIE AFDAESMRLGFSSMALGGIP AEAAAQGYPAPPPAPAWPSR PVPRSGAHWSHGPPPPGMPP PAWRXPALLAPPGPPNWLGP PTQSGTPSGGALLLLRNSPQ PLKRAASGGSSGENVGPPAA VPGPLSRSTSVASHILNGSS HFYS in isoform 2. 1 PublicationAdd BLAST304
Alternative sequenceiVSP_0405471051 – 1235Missing in isoform 2. 1 PublicationAdd BLAST185

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF140556 mRNA. Translation: AAD39480.2.
AB290408 mRNA. Translation: BAF64457.1.
RefSeqiNP_073193.1. NM_022702.1. [Q9JLS3-1]
UniGeneiRn.9575.

Genome annotation databases

GeneIDi64666.
KEGGirno:64666.
UCSCiRGD:621590. rat. [Q9JLS3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF140556 mRNA. Translation: AAD39480.2.
AB290408 mRNA. Translation: BAF64457.1.
RefSeqiNP_073193.1. NM_022702.1. [Q9JLS3-1]
UniGeneiRn.9575.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U5QX-ray2.10A/B1-320[»]
1U5RX-ray2.10A/B1-320[»]
2GCDX-ray2.55A/B12-320[»]
ProteinModelPortaliQ9JLS3.
SMRiQ9JLS3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JLS3. 1 interactor.
STRINGi10116.ENSRNOP00000061854.

PTM databases

iPTMnetiQ9JLS3.
PhosphoSitePlusiQ9JLS3.

Proteomic databases

PaxDbiQ9JLS3.
PRIDEiQ9JLS3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi64666.
KEGGirno:64666.
UCSCiRGD:621590. rat. [Q9JLS3-1]

Organism-specific databases

CTDi9344.
RGDi621590. Taok2.

Phylogenomic databases

eggNOGiKOG0577. Eukaryota.
ENOG410Y259. LUCA.
HOGENOMiHOG000236358.
HOVERGENiHBG088996.
InParanoidiQ9JLS3.
KOiK04429.
PhylomeDBiQ9JLS3.

Miscellaneous databases

EvolutionaryTraceiQ9JLS3.
PROiQ9JLS3.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTAOK2_RAT
AccessioniPrimary (citable) accession number: Q9JLS3
Secondary accession number(s): A6BM05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.