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Protein

ARF GTPase-activating protein GIT2

Gene

Git2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein for the ADP ribosylation factor family.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri11 – 34C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein complex binding Source: MGI

GO - Biological processi

  • behavioral response to pain Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
ARF GTPase-activating protein GIT2
Short name:
ARF GAP GIT2
Alternative name(s):
Cool-interacting tyrosine-phosphorylated protein 2
Short name:
CAT-2
Short name:
CAT2
G protein-coupled receptor kinase-interactor 2
GRK-interacting protein 2
Gene namesi
Name:Git2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1347053. Git2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000742041 – 708ARF GTPase-activating protein GIT2Add BLAST708

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei224PhosphotyrosineBy similarity1
Modified residuei383PhosphoserineBy similarity1
Modified residuei391PhosphotyrosineBy similarity1
Modified residuei393PhosphoserineCombined sources1
Modified residuei396PhosphoserineCombined sources1
Modified residuei400PhosphothreonineCombined sources1
Modified residuei437PhosphothreonineBy similarity1
Modified residuei455PhosphoserineBy similarity1
Modified residuei505PhosphoserineBy similarity1
Modified residuei508PhosphoserineBy similarity1
Modified residuei511PhosphoserineCombined sources1
Modified residuei519PhosphoserineCombined sources1
Modified residuei535PhosphoserineBy similarity1
Modified residuei536PhosphothreonineCombined sources1
Modified residuei539PhosphoserineBy similarity1
Modified residuei544PhosphothreonineBy similarity1
Modified residuei563PhosphoserineCombined sources1

Post-translational modificationi

Tyrosine phosphorylated when coexpressed in cells with PTK2/FAK1 and SRC.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9JLQ2.
MaxQBiQ9JLQ2.
PaxDbiQ9JLQ2.
PeptideAtlasiQ9JLQ2.
PRIDEiQ9JLQ2.

PTM databases

iPTMnetiQ9JLQ2.
PhosphoSitePlusiQ9JLQ2.

Miscellaneous databases

PMAP-CutDBQ9JLQ2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000041890.
CleanExiMM_GIT2.
ExpressionAtlasiQ9JLQ2. baseline and differential.
GenevisibleiQ9JLQ2. MM.

Interactioni

Subunit structurei

Interacts with G protein-coupled receptor kinases. Associates with PXN. Also interacts with PIX exchange factors. Identified in a complex with ARHGEF6 and BIN2 (By similarity). Interacts with TGFB1I1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Arhgef7Q9ES286EBI-642860,EBI-642580

GO - Molecular functioni

  • protein complex binding Source: MGI

Protein-protein interaction databases

BioGridi204983. 3 interactors.
IntActiQ9JLQ2. 4 interactors.
MINTiMINT-1751173.
STRINGi10090.ENSMUSP00000107803.

Structurei

3D structure databases

ProteinModelPortaliQ9JLQ2.
SMRiQ9JLQ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 124Arf-GAPPROSITE-ProRule annotationAdd BLAST124
Repeati132 – 161ANK 1Add BLAST30
Repeati166 – 195ANK 2Add BLAST30
Repeati199 – 228ANK 3Add BLAST30

Sequence similaritiesi

Contains 3 ANK repeats.PROSITE-ProRule annotation
Contains 1 Arf-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri11 – 34C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0818. Eukaryota.
ENOG410XR8U. LUCA.
GeneTreeiENSGT00860000133715.
HOGENOMiHOG000232135.
HOVERGENiHBG012506.
InParanoidiQ9JLQ2.
KOiK12487.
TreeFamiTF317762.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR022018. GIT1_C.
IPR013724. GIT_SHD.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF12205. GIT1_C. 1 hit.
PF08518. GIT_SHD. 2 hits.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 3 hits.
SM00105. ArfGap. 1 hit.
SM00555. GIT. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JLQ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRLRSSDV CADCNGPDPS WASVNRGTFI CDECCSVHRS LGRHISQVRH
60 70 80 90 100
LKHTAWPPTL LQMVETLYNN GANSIWEHSL LDPASIMSGR RKANPQDKVH
110 120 130 140 150
PNKAEFIRAK YQMLAFVHRL PCREDDSVTA KDLSKQLHSS VRTGNLETCL
160 170 180 190 200
RLLSLGAQAN FFHPEKGSTP LHVASKAGQI LQAELLAVYG ADPGTQDSSG
210 220 230 240 250
KTPVDYARQG GHHELAERLI EIQYELTDRL AFYLCGRKPD HKSGQHFLIP
260 270 280 290 300
QRADSLDLSE LAKAAKKKLQ SLSNHLFEEL AMDVYDEVDR RETDAVWLAT
310 320 330 340 350
QNHSTLVTET TVVPFLPVNP EYSSTRNQGR QKLARFNAHE FATLVIDILS
360 370 380 390 400
DAKRRQQGSP LSRSKDNVEL ILRTVSTQHS TESQDNDQPD YDSVASDEDT
410 420 430 440 450
DVETRASKAN RQKLQTLQSE NSSLRRQATA SACQVQTGSD HKDTASHSSL
460 470 480 490 500
KRRPSARGSR PMSMYETGSG QKPYLPMGEA SHPEESRTRL QPFPTHIGRS
510 520 530 540 550
ALVTSSSSLP SFPSTLSWSR DESARRASRL EKQNSTPESD YDNTACDPEP
560 570 580 590 600
DDTGSTRKGR QRSMLWQGDG LLPDTAEPHS VPSPTLPSTE DVIRKTEQIT
610 620 630 640 650
KNIQELLRAA QENKHDSYIP CSERIHVAVT EMAALFPKKP KSDTVRTSLR
660 670 680 690 700
LLTSSAYRLQ SECRKALPGD SSLPTDVQLV TQQVIQCAYD IAKAAKQLVT

ITTKENSS
Length:708
Mass (Da):78,766
Last modified:July 27, 2011 - v2
Checksum:iB84F4F684182D8CA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti188V → E in AAF61633 (PubMed:10428811).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148693 mRNA. Translation: AAF61633.1.
AC087330 Genomic DNA. No translation available.
CCDSiCCDS19570.2.
RefSeqiNP_062808.3. NM_019834.3.
UniGeneiMm.195632.

Genome annotation databases

EnsembliENSMUST00000112185; ENSMUSP00000107803; ENSMUSG00000041890.
GeneIDi26431.
KEGGimmu:26431.
UCSCiuc008yzz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148693 mRNA. Translation: AAF61633.1.
AC087330 Genomic DNA. No translation available.
CCDSiCCDS19570.2.
RefSeqiNP_062808.3. NM_019834.3.
UniGeneiMm.195632.

3D structure databases

ProteinModelPortaliQ9JLQ2.
SMRiQ9JLQ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204983. 3 interactors.
IntActiQ9JLQ2. 4 interactors.
MINTiMINT-1751173.
STRINGi10090.ENSMUSP00000107803.

PTM databases

iPTMnetiQ9JLQ2.
PhosphoSitePlusiQ9JLQ2.

Proteomic databases

EPDiQ9JLQ2.
MaxQBiQ9JLQ2.
PaxDbiQ9JLQ2.
PeptideAtlasiQ9JLQ2.
PRIDEiQ9JLQ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000112185; ENSMUSP00000107803; ENSMUSG00000041890.
GeneIDi26431.
KEGGimmu:26431.
UCSCiuc008yzz.1. mouse.

Organism-specific databases

CTDi9815.
MGIiMGI:1347053. Git2.

Phylogenomic databases

eggNOGiKOG0818. Eukaryota.
ENOG410XR8U. LUCA.
GeneTreeiENSGT00860000133715.
HOGENOMiHOG000232135.
HOVERGENiHBG012506.
InParanoidiQ9JLQ2.
KOiK12487.
TreeFamiTF317762.

Miscellaneous databases

PMAP-CutDBQ9JLQ2.
PROiQ9JLQ2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041890.
CleanExiMM_GIT2.
ExpressionAtlasiQ9JLQ2. baseline and differential.
GenevisibleiQ9JLQ2. MM.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR022018. GIT1_C.
IPR013724. GIT_SHD.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF12205. GIT1_C. 1 hit.
PF08518. GIT_SHD. 2 hits.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 3 hits.
SM00105. ArfGap. 1 hit.
SM00555. GIT. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGIT2_MOUSE
AccessioniPrimary (citable) accession number: Q9JLQ2
Secondary accession number(s): E9QPU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.