Q9JLQ2 (GIT2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ARF GTPase-activating protein GIT2
Short name=ARF GAP GIT2

Alternative name(s):
Cool-interacting tyrosine-phosphorylated protein 2
Short name=CAT-2
Short name=CAT2
G protein-coupled receptor kinase-interactor 2
GRK-interacting protein 2

Gene names

Name:

Git2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	708 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GTPase-activating protein for the ADP ribosylation factor family By similarity.
Subunit structure	Interacts with G protein-coupled receptor kinases. Associates with PXN. Also interacts with PIX exchange factors. Identified in a complex with ARHGEF6 and BIN2 By similarity. Interacts with TGFB1I1. Ref.3
Post-translational modification	Tyrosine phosphorylated when coexpressed in cells with PTK2/FAK1 and SRC.
Sequence similarities	Contains 3 ANK repeats. Contains 1 Arf-GAP domain.

Ontologies

Keywords
Domain	ANK repeat Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of GTPase activity Inferred from electronic annotation. Source: GOC regulation of ARF GTPase activity Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleoplasm Inferred from electronic annotation. Source: Compara
Molecular_function	ARF GTPase activator activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Arhgef7	Q9ES28	6	EBI-642860,EBI-642580

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 708	708	ARF GTPase-activating protein GIT2		PRO_0000074204
Regions
	Domain	1 – 124	124	Arf-GAP
	Repeat	132 – 161	30	ANK 1
	Repeat	166 – 195	30	ANK 2
	Repeat	199 – 228	30	ANK 3
	Zinc finger	11 – 34	24	C4-type
Amino acid modifications
	Modified residue	393	1	Phosphoserine By similarity
	Modified residue	396	1	Phosphoserine Ref.4
	Modified residue	400	1	Phosphothreonine Ref.4
	Modified residue	508	1	Phosphoserine By similarity
	Modified residue	535	1	Phosphoserine Ref.4
	Modified residue	563	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	188	1	V → E in AAF61633. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9JLQ2 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: B84F4F684182D8CA	FASTA	708	78,766
	10 20 30 40 50 60 MSKRLRSSDV CADCNGPDPS WASVNRGTFI CDECCSVHRS LGRHISQVRH LKHTAWPPTL 70 80 90 100 110 120 LQMVETLYNN GANSIWEHSL LDPASIMSGR RKANPQDKVH PNKAEFIRAK YQMLAFVHRL 130 140 150 160 170 180 PCREDDSVTA KDLSKQLHSS VRTGNLETCL RLLSLGAQAN FFHPEKGSTP LHVASKAGQI 190 200 210 220 230 240 LQAELLAVYG ADPGTQDSSG KTPVDYARQG GHHELAERLI EIQYELTDRL AFYLCGRKPD 250 260 270 280 290 300 HKSGQHFLIP QRADSLDLSE LAKAAKKKLQ SLSNHLFEEL AMDVYDEVDR RETDAVWLAT 310 320 330 340 350 360 QNHSTLVTET TVVPFLPVNP EYSSTRNQGR QKLARFNAHE FATLVIDILS DAKRRQQGSP 370 380 390 400 410 420 LSRSKDNVEL ILRTVSTQHS TESQDNDQPD YDSVASDEDT DVETRASKAN RQKLQTLQSE 430 440 450 460 470 480 NSSLRRQATA SACQVQTGSD HKDTASHSSL KRRPSARGSR PMSMYETGSG QKPYLPMGEA 490 500 510 520 530 540 SHPEESRTRL QPFPTHIGRS ALVTSSSSLP SFPSTLSWSR DESARRASRL EKQNSTPESD 550 560 570 580 590 600 YDNTACDPEP DDTGSTRKGR QRSMLWQGDG LLPDTAEPHS VPSPTLPSTE DVIRKTEQIT 610 620 630 640 650 660 KNIQELLRAA QENKHDSYIP CSERIHVAVT EMAALFPKKP KSDTVRTSLR LLTSSAYRLQ 670 680 690 700 SECRKALPGD SSLPTDVQLV TQQVIQCAYD IAKAAKQLVT ITTKENSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A tyrosine-phosphorylated protein that binds to an important regulatory region on the cool family of p21-activated kinase-binding proteins." Bagrodia S., Bailey D., Lenard Z., Hart M., Guan J.L., Premont R.T., Taylor S.J., Cerione R.A. J. Biol. Chem. 274:22393-22400(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: a role in cytoskeletal remodeling." Turner C.E., Brown M.C., Perrotta J.A., Riedy M.C., Nikolopoulos S.N., McDonald A.R., Bagrodia S., Thomas S.M., Leventhal P.S. J. Cell Biol. 145:851-863(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TGFB1I1.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-396; THR-400 AND SER-535, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF148693 mRNA. Translation: AAF61633.1. AC087330 Genomic DNA. No translation available.
IPI	IPI00124597.
RefSeq	NP_062808.3. NM_019834.3.
UniGene	Mm.195632.
3D structure databases
ProteinModelPortal	Q9JLQ2.
SMR	Q9JLQ2. Positions 3-221, 582-705.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9JLQ2. 2 interactions.
PTM databases
PhosphoSite	Q9JLQ2.
Proteomic databases
PaxDb	Q9JLQ2.
PRIDE	Q9JLQ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000112185; ENSMUSP00000107803; ENSMUSG00000041890.
GeneID	26431.
KEGG	mmu:26431.
UCSC	uc008yzz.1. mouse.
Organism-specific databases
CTD	9815.
MGI	MGI:1347053. Git2.
Phylogenomic databases
eggNOG	COG5347.
GeneTree	ENSGT00700000104126.
HOGENOM	HOG000232135.
HOVERGEN	HBG012506.
KO	K12487.
OrthoDB	EOG45X7VM.
Gene expression databases
ArrayExpress	Q9JLQ2.
Bgee	Q9JLQ2.
CleanEx	MM_GIT2.
Genevestigator	Q9JLQ2.
GermOnline	ENSMUSG00000041890. Mus musculus.
Family and domain databases
Gene3D	1.25.40.20. 2 hits.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR001164. ArfGAP. IPR022018. GIT1_C. IPR013724. GIT_SHD. [Graphical view]
Pfam	PF12796. Ank_2. 1 hit. PF01412. ArfGap. 1 hit. PF12205. GIT1_C. 1 hit. PF08518. GIT_SHD. 2 hits. [Graphical view]
PRINTS	PR00405. REVINTRACTNG.
SMART	SM00248. ANK. 3 hits. SM00105. ArfGap. 1 hit. SM00555. GIT. 2 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 1 hit. PS50115. ARFGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	304481.
PMAP-CutDB	Q9JLQ2.
SOURCE	Search...

Entry information

Entry name

GIT2_MOUSE

Accession

Primary (citable) accession number: Q9JLQ2
Secondary accession number(s): E9QPU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program