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Q9JLQ0

- CD2AP_MOUSE

UniProt

Q9JLQ0 - CD2AP_MOUSE

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Protein
CD2-associated protein
Gene
Cd2ap, Mets1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. In collaboration with CBLC, modulates the rate of RET turnover and may act as regulatory checkpoint that limits the potency of GDNF on neuronal survival. Controls CBLC function, converting it from an inhibitor to a promoter of RET degradation. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis.1 Publication

GO - Molecular functioni

  1. protein binding Source: MGI

GO - Biological processi

  1. cell migration Source: Ensembl
  2. mitotic nuclear division Source: UniProtKB-KW
  3. negative regulation of transforming growth factor beta1 production Source: MGI
  4. positive regulation of protein localization to nucleus Source: MGI
  5. proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
  6. regulation of actin cytoskeleton reorganization Source: MGI
  7. regulation of receptor-mediated endocytosis Source: Ensembl
  8. single organismal cell-cell adhesion Source: Ensembl
  9. vesicle organization Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
CD2-associated protein
Alternative name(s):
Mesenchyme-to-epithelium transition protein with SH3 domains 1
Short name:
METS-1
Gene namesi
Name:Cd2ap
Synonyms:Mets1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1330281. Cd2ap.

Subcellular locationi

Cytoplasmcytoskeleton. Cell projectionruffle
Note: During late anaphase and telophase, concentrates in the vicinity of the midzone microtubules and in the midbody in late telophase By similarity. Located at podocyte slit diaphragm between podocyte foot processes.2 Publications

GO - Cellular componenti

  1. cell cortex Source: MGI
  2. cell-cell junction Source: MGI
  3. cytoplasm Source: MGI
  4. cytosol Source: Reactome
  5. endocytic vesicle Source: Ensembl
  6. filamentous actin Source: Ensembl
  7. nucleolus Source: Ensembl
  8. perinuclear region of cytoplasm Source: Ensembl
  9. plasma membrane Source: MGI
  10. ruffle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Death at 6 to 7 weeks of age from renal failure. Mice show defects in epithelial foot processes, accompanied by mesangial cell hyperplasia and extracellular matrix deposition.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 637637CD2-associated protein
PRO_0000089436Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei224 – 2241Phosphoserine By similarity
Modified residuei458 – 4581Phosphoserine1 Publication
Modified residuei510 – 5101Phosphoserine By similarity
Modified residuei514 – 5141Phosphoserine By similarity

Post-translational modificationi

Phosphorylated on tyrosine residues; probably by c-Abl, Fyn and c-Src By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9JLQ0.
PaxDbiQ9JLQ0.
PRIDEiQ9JLQ0.

PTM databases

PhosphoSiteiQ9JLQ0.

Expressioni

Tissue specificityi

Expressed in podocytes (at protein level).1 Publication

Gene expression databases

BgeeiQ9JLQ0.
CleanExiMM_CD2AP.
GenevestigatoriQ9JLQ0.

Interactioni

Subunit structurei

Self-associates. Homodimer Reviewed prediction. Interacts with F-actin, PKD2, NPHS1 and NPHS2. Interacts with WTIP. Interacts with DDN; interaction is direct. Interacts (via SH3 2 domain) with CBL (via phosphorylated C-terminus). Interacts with BCAR1/p130Cas (via SH3 domain). Interacts with MVB12A and ARHGAP17. Interacts with ANLN, CD2 and CBLB. Interacts with PDCD6IP and TSG101. Interacts with RIN3. Interacts directly with RET (inactive) and CBLC; upon RET activation by GDNF suggested to dissociate from RET as CBLC:CD2AP complex.9 Publications

Protein-protein interaction databases

BioGridi198584. 19 interactions.
IntActiQ9JLQ0. 20 interactions.
MINTiMINT-255809.
STRINGi10090.ENSMUSP00000024709.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 63
Beta strandi8 – 103
Beta strandi14 – 174
Beta strandi25 – 317
Beta strandi37 – 426
Beta strandi45 – 506
Helixi51 – 533
Beta strandi54 – 563
Beta strandi113 – 1153
Beta strandi134 – 1429
Beta strandi145 – 1506
Beta strandi153 – 1586
Turni159 – 1613
Beta strandi270 – 2789
Beta strandi283 – 2875
Beta strandi295 – 3017
Beta strandi303 – 31311
Beta strandi316 – 3216
Helixi322 – 3243
Beta strandi325 – 3295

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JTENMR-A270-329[»]
2KRMNMR-A2-58[»]
2KRNNMR-A111-166[»]
2KRONMR-A270-329[»]
2LZ6NMR-B270-329[»]
2MCNNMR-A2-58[»]
ProteinModelPortaliQ9JLQ0.
SMRiQ9JLQ0. Positions 2-329, 475-503.

Miscellaneous databases

EvolutionaryTraceiQ9JLQ0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 5959SH3 1; truncated
Add
BLAST
Domaini108 – 16760SH3 2
Add
BLAST
Domaini269 – 33062SH3 3
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 175175Interaction with ANLN and localization to the midbody By similarity
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili578 – 63659 Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi336 – 35217SH3-binding Reviewed prediction
Add
BLAST
Motifi378 – 39720SH3-binding Reviewed prediction
Add
BLAST
Motifi410 – 42213SH3-binding Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi336 – 42287Pro-rich
Add
BLAST

Domaini

Potential homodimerization is mediated by the coiled coil domain By similarity.

Sequence similaritiesi

Contains 3 SH3 domains.

Keywords - Domaini

Coiled coil, Repeat, SH3 domain, SH3-binding

Phylogenomic databases

eggNOGiNOG319250.
GeneTreeiENSGT00530000063594.
HOGENOMiHOG000231405.
HOVERGENiHBG057824.
InParanoidiQ9JLQ0.
KOiK13738.
OMAiVHDDELT.
OrthoDBiEOG7W41BC.
TreeFamiTF350191.

Family and domain databases

InterProiIPR028445. CD2AP.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR14167:SF23. PTHR14167:SF23. 1 hit.
PfamiPF00018. SH3_1. 1 hit.
PF14604. SH3_9. 2 hits.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 3 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
PROSITEiPS50002. SH3. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JLQ0-1 [UniParc]FASTAAdd to Basket

« Hide

MVDYIVEYDY DAVHDDELTI RVGEIIRNVK KLQEEGWLEG ELNGRRGMFP    50
DNFVKEIKRE TEPKDDNLPI KRERQGNVAS LVQRISTYGL PAGGIQPHPQ 100
TKAIKKKTKK RQCKVLFDYS PQNEDELELI VGDVIDVIEE VEEGWWSGTL 150
NNKLGLFPSN FVKELESTED GETHNAQEES EVPLTGPTSP LPSPGNGSEP 200
APGSVAQPKK IRGIGFGDIF KEGSVKLRTR TSSSETEEKK TEKPLILQPL 250
GSRTQNVEVT KPDVDGKIKA KEYCRTLFPY TGTNEDELTF REGEIIHLIS 300
KETGEAGWWK GELNGKEGVF PDNFAVQISE LDKDFPKPKK PPPPAKGPAP 350
KPDLSAAEKK AFPLKAEEKD EKSLLEQKPS KPAAPQVPPK KPTAPTKASN 400
LLRSPGAVYP KRPEKPVPPP PPAAKINGEV SIISSKIDTE PVSKPKLDPE 450
QLPVRPKSVD LDAFVARNSK ETDDVNFDDI ASSENLLHLT ANRPKMPGRR 500
LPGRFNGGHS PTQSPEKTLK LPKEDDSGNL KPLEFKKDAS YSSKSSLSTP 550
SSASKVNTAA FLTPLELKAK AEADDGKRNS VDELRAQIIE LLCIVDALKK 600
DHGKELEKLR KELEEEKAMR SNLEVEIAKL KKAVLLS 637
Length:637
Mass (Da):70,450
Last modified:July 27, 2011 - v3
Checksum:i0B618FE82AF12332
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti78 – 781V → E in AAF73150. 1 Publication
Sequence conflicti107 – 1071Missing in AAC36099. 1 Publication
Sequence conflicti110 – 1101K → Q in AAC36099. 1 Publication
Sequence conflicti244 – 2441P → R in AAC36099. 1 Publication
Sequence conflicti295 – 2973IIH → LS in AAC36099. 1 Publication
Sequence conflicti392 – 3921P → PTAPTKA in AAC36099. 1 Publication
Sequence conflicti545 – 5451S → P in AAF73150. 1 Publication
Sequence conflicti545 – 5451S → P in CAD30510. 1 Publication
Sequence conflicti545 – 5451S → P in AAH19744. 1 Publication
Sequence conflicti578 – 5781R → K in AAF73150. 1 Publication
Sequence conflicti578 – 5781R → K in CAD30510. 1 Publication
Sequence conflicti578 – 5781R → K in AAH19744. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF077003 mRNA. Translation: AAC36099.1.
AF149092 mRNA. Translation: AAF73150.1.
AJ459109 mRNA. Translation: CAD30510.1.
AC111082 Genomic DNA. No translation available.
BC019744 mRNA. Translation: AAH19744.1.
CCDSiCCDS50114.1.
RefSeqiNP_033977.3. NM_009847.3.
UniGeneiMm.218637.

Genome annotation databases

EnsembliENSMUST00000024709; ENSMUSP00000024709; ENSMUSG00000061665.
GeneIDi12488.
KEGGimmu:12488.
UCSCiuc008cot.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF077003 mRNA. Translation: AAC36099.1 .
AF149092 mRNA. Translation: AAF73150.1 .
AJ459109 mRNA. Translation: CAD30510.1 .
AC111082 Genomic DNA. No translation available.
BC019744 mRNA. Translation: AAH19744.1 .
CCDSi CCDS50114.1.
RefSeqi NP_033977.3. NM_009847.3.
UniGenei Mm.218637.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2JTE NMR - A 270-329 [» ]
2KRM NMR - A 2-58 [» ]
2KRN NMR - A 111-166 [» ]
2KRO NMR - A 270-329 [» ]
2LZ6 NMR - B 270-329 [» ]
2MCN NMR - A 2-58 [» ]
ProteinModelPortali Q9JLQ0.
SMRi Q9JLQ0. Positions 2-329, 475-503.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198584. 19 interactions.
IntActi Q9JLQ0. 20 interactions.
MINTi MINT-255809.
STRINGi 10090.ENSMUSP00000024709.

PTM databases

PhosphoSitei Q9JLQ0.

Proteomic databases

MaxQBi Q9JLQ0.
PaxDbi Q9JLQ0.
PRIDEi Q9JLQ0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000024709 ; ENSMUSP00000024709 ; ENSMUSG00000061665 .
GeneIDi 12488.
KEGGi mmu:12488.
UCSCi uc008cot.1. mouse.

Organism-specific databases

CTDi 23607.
MGIi MGI:1330281. Cd2ap.

Phylogenomic databases

eggNOGi NOG319250.
GeneTreei ENSGT00530000063594.
HOGENOMi HOG000231405.
HOVERGENi HBG057824.
InParanoidi Q9JLQ0.
KOi K13738.
OMAi VHDDELT.
OrthoDBi EOG7W41BC.
TreeFami TF350191.

Miscellaneous databases

ChiTaRSi CD2AP. mouse.
EvolutionaryTracei Q9JLQ0.
NextBioi 281400.
PROi Q9JLQ0.
SOURCEi Search...

Gene expression databases

Bgeei Q9JLQ0.
CleanExi MM_CD2AP.
Genevestigatori Q9JLQ0.

Family and domain databases

InterProi IPR028445. CD2AP.
IPR001452. SH3_domain.
[Graphical view ]
PANTHERi PTHR14167:SF23. PTHR14167:SF23. 1 hit.
Pfami PF00018. SH3_1. 1 hit.
PF14604. SH3_9. 2 hits.
[Graphical view ]
PRINTSi PR00452. SH3DOMAIN.
SMARTi SM00326. SH3. 3 hits.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 3 hits.
PROSITEi PS50002. SH3. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel adaptor protein orchestrates receptor patterning and cytoskeletal polarity in T-cell contacts."
    Dustin M.L., Olszowy M.W., Holdorf A.D., Li J., Bromley S., Desai N., Widder P., Rosenberger F., van der Merwe P.A., Allen P.M., Shaw A.S.
    Cell 94:667-677(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CD2.
  2. "In vivo interaction of the adapter protein CD2-associated protein with the type 2 polycystic kidney disease protein, polycystin-2."
    Lehtonen S., Ora A., Olkkonen V.M., Geng L., Zerial M., Somlo S., Lehtonen E.
    J. Biol. Chem. 275:32888-32893(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH PKD2.
  3. "Role of the interaction between CD2AP and c-Cbl."
    Meton I., Le Marchand-Brustel Y., Cormont M.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 394-637.
    Tissue: Mammary tumor.
  6. "Congenital nephrotic syndrome in mice lacking CD2-associated protein."
    Shih N.Y., Li J., Karpitskii V., Nguyen A., Dustin M.L., Kanagawa O., Miner J.H., Shaw A.S.
    Science 286:312-315(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH NPHS1, DISRUPTION PHENOTYPE.
  7. "CD2AP localizes to the slit diaphragm and binds to nephrin via a novel C-terminal domain."
    Shih N.Y., Li J., Cotran R., Mundel P., Miner J.H., Shaw A.S.
    Am. J. Pathol. 159:2303-2308(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH NPHS1.
  8. "Podocin, a raft-associated component of the glomerular slit diaphragm, interacts with CD2AP and nephrin."
    Schwarz K., Simons M., Reiser J., Saleem M.A., Faul C., Kriz W., Shaw A.S., Holzman L.B., Mundel P.
    J. Clin. Invest. 108:1621-1629(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NPHS1 AND NPHS2.
  9. "CD2-associated protein directly interacts with the actin cytoskeleton."
    Lehtonen S., Zhao F., Lehtonen E.
    Am. J. Physiol. 283:F734-F743(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH F-ACTIN.
  10. Cited for: INTERACTION WITH WTIP.
  11. "Nuclear relocation of the nephrin and CD2AP-binding protein dendrin promotes apoptosis of podocytes."
    Asanuma K., Campbell K.N., Kim K., Faul C., Mundel P.
    Proc. Natl. Acad. Sci. U.S.A. 104:10134-10139(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DDN.
  12. "CD2AP and Cbl-3/Cbl-c constitute a critical checkpoint in the regulation of ret signal transduction."
    Tsui C.C., Pierchala B.A.
    J. Neurosci. 28:8789-8800(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RET, TISSUE SPECIFICITY.
  13. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. Cited for: STRUCTURE BY NMR OF 270-329.

Entry informationi

Entry nameiCD2AP_MOUSE
AccessioniPrimary (citable) accession number: Q9JLQ0
Secondary accession number(s): E9QL86
, O88903, Q8K4Z1, Q8VCI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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