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Q9JLN6

- ADA28_MOUSE

UniProt

Q9JLN6 - ADA28_MOUSE

Protein

Disintegrin and metalloproteinase domain-containing protein 28

Gene

Adam28

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 3 (12 Feb 2003)
      Previous versions | rss
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    Functioni

    May play a role in organogenesis and organ-specific functions such as thymic T-cell development.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi171 – 1711Zinc; in inhibited formBy similarity
    Metal bindingi342 – 3421Zinc; catalyticBy similarity
    Active sitei343 – 3431PROSITE-ProRule annotation
    Metal bindingi346 – 3461Zinc; catalyticBy similarity
    Metal bindingi352 – 3521Zinc; catalyticBy similarity

    GO - Molecular functioni

    1. metalloendopeptidase activity Source: InterPro
    2. zinc ion binding Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM12.020.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Disintegrin and metalloproteinase domain-containing protein 28 (EC:3.4.24.-)
    Short name:
    ADAM 28
    Alternative name(s):
    Thymic epithelial cell-ADAM
    Short name:
    TECADAM
    Gene namesi
    Name:Adam28
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:105988. Adam28.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi343 – 3431E → A: Abolishes prodomain removal. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Propeptidei21 – 195175By similarityPRO_0000029132Add
    BLAST
    Chaini196 – 793598Disintegrin and metalloproteinase domain-containing protein 28PRO_0000029133Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi91 – 911N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi272 – 2721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi277 – 2771N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi317 ↔ 397By similarity
    Disulfide bondi357 ↔ 381By similarity
    Disulfide bondi359 ↔ 364By similarity
    Disulfide bondi468 ↔ 488By similarity
    Glycosylationi531 – 5311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi551 – 5511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi605 – 6051N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi631 – 6311N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi632 ↔ 642By similarity
    Disulfide bondi636 ↔ 648By similarity
    Disulfide bondi650 ↔ 659By similarity

    Post-translational modificationi

    Pro-domain removal and maturation may be, at least in part, autocatalytic.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiQ9JLN6.
    PRIDEiQ9JLN6.

    PTM databases

    PhosphoSiteiQ9JLN6.

    Miscellaneous databases

    PMAP-CutDBQ9JLN6.

    Expressioni

    Tissue specificityi

    Strong expression in thymic epithelial cells and developmentally related tissues including the trachea, thyroid, lung and stomach, but not in lymphocytes. Expressed at high levels also in epididymis. In contrast with human is not expressed in immature or mature lymphocyte populations of thymocytes, lymph node, spleen, and bone marrow.1 Publication

    Developmental stagei

    The expression patterns in adult and day 15.5 embryos are similar.

    Gene expression databases

    ArrayExpressiQ9JLN6.
    BgeeiQ9JLN6.
    CleanExiMM_ADAM28.
    GenevestigatoriQ9JLN6.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JLN6.
    SMRiQ9JLN6. Positions 204-623, 633-660.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini196 – 668473ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini690 – 793104CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei669 – 68921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini206 – 402197Peptidase M12BPROSITE-ProRule annotationAdd
    BLAST
    Domaini410 – 49687DisintegrinPROSITE-ProRule annotationAdd
    BLAST
    Domaini628 – 66033EGF-likePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi169 – 1768Cysteine switchBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi497 – 631135Cys-richAdd
    BLAST
    Compositional biasi760 – 7656Poly-Pro

    Domaini

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

    Sequence similaritiesi

    Contains 1 disintegrin domain.PROSITE-ProRule annotation
    Contains 1 EGF-like domain.PROSITE-ProRule annotation
    Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG277164.
    GeneTreeiENSGT00740000114848.
    HOGENOMiHOG000230883.
    HOVERGENiHBG006978.
    InParanoidiQ9JLN6.
    KOiK08614.
    OMAiTSPQIMD.
    OrthoDBiEOG7PGDQ7.
    PhylomeDBiQ9JLN6.
    TreeFamiTF314733.

    Family and domain databases

    Gene3Di3.40.390.10. 1 hit.
    4.10.70.10. 1 hit.
    InterProiIPR006586. ADAM_Cys-rich.
    IPR001762. Blood-coag_inhib_Disintegrin.
    IPR018358. Disintegrin_CS.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR002870. Peptidase_M12B_N.
    [Graphical view]
    PfamiPF08516. ADAM_CR. 1 hit.
    PF00200. Disintegrin. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    [Graphical view]
    PRINTSiPR00289. DISINTEGRIN.
    SMARTiSM00608. ACR. 1 hit.
    SM00050. DISIN. 1 hit.
    SM00181. EGF. 1 hit.
    [Graphical view]
    SUPFAMiSSF57552. SSF57552. 1 hit.
    PROSITEiPS50215. ADAM_MEPRO. 1 hit.
    PS00427. DISINTEGRIN_1. 1 hit.
    PS50214. DISINTEGRIN_2. 1 hit.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform 1 (identifier: Q9JLN6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MQQWSLLVVS FLLSPVPVSA IKELPKAKKY EVVYPIRLHP LRKRETQEPE    50
    PKETFETELR YKMTVNGKVA VLYLKKNNKL LAPDYSETYY NSSGNKVTTS 100
    PQIMDSCYYQ GHIVNEKVSA ASISTCQGLR GYISQGDEKY FIEPLSSENL 150
    DEQAHALFKD DSNEDQEKSN CGVDDALWLQ GLHQDVALPA TRLIKLNDGM 200
    VQEPKKYIEY YVVLDNGEFK KYNKNLAEIR KIVLEMANYI NMLYNKLDAH 250
    VALVGVEIWT DGDKIKITPD ANTTLENFSK WRGNDLLKRK HHDIAQLISS 300
    TDFSGSTVGL AFMSSMCSPY HSVGIVQDHS NYHLRVAGTM AHEMGHNLGM 350
    IHDYLSCKCP SEVCVMEQSL RFHMPTDFSS CSRVNYKQFL EEKLSHCLFN 400
    SPLPSDIIST PVCGNQLLEM NEDCDCGTPK ECTNKCCDAR TCKIKAGFQC 450
    ALGECCEKCQ LKKPGVVCRA AKDECDLPEV CDGKSSHCPG DRFRVNGSPC 500
    QNGHGYCLKG KCPTLQQQCM DMWGPGTKVA NTSCYKQNEG GTKYGYCHVE 550
    NGTHMPCKAK DAMCGKLFCE GGSGDLPWKG LTISFLTCKL FDPEDTSQGV 600
    DMVANGTKCG TNKVCINAEC VDMEKTYKSA NCSSKCKGHA VCDHELQCQC 650
    KEGWAPPDCE NSATVFHFSI VVGVLFPLAV IFVVVAIVIQ RQSARRKQRR 700
    VQRLPSTKDA KLHNQKCRPQ KVKDVQPQEM SQMKKLHVSD LPSEEPEPPP 750
    DVLITKPNFP PPPIPVSLTG RAKVPFVKTP HPFSQQIGRV YLK 793
    Length:793
    Mass (Da):88,671
    Last modified:February 12, 2003 - v3
    Checksum:i7715E71456D4403B
    GO
    Isoform 2 (identifier: Q9JLN6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         769-793: Missing.

    Show »
    Length:768
    Mass (Da):85,833
    Checksum:i6786AB68FD631A36
    GO
    Isoform 3 (identifier: Q9JLN6-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         769-771: TGR → DPN
         775-793: Missing.

    Show »
    Length:774
    Mass (Da):86,458
    Checksum:i93C42E7C92A20786
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei769 – 79325Missing in isoform 2. 1 PublicationVSP_005488Add
    BLAST
    Alternative sequencei769 – 7713TGR → DPN in isoform 3. 3 PublicationsVSP_005489
    Alternative sequencei775 – 79319Missing in isoform 3. 3 PublicationsVSP_005490Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF153350 mRNA. Translation: AAF71993.1.
    AF163290 mRNA. Translation: AAM21935.1.
    AF163291 mRNA. Translation: AAM21936.1.
    AF163292 mRNA. Translation: AAM21937.1.
    BC058782 mRNA. Translation: AAH58782.1.
    CCDSiCCDS36964.1. [Q9JLN6-3]
    CCDS36965.1. [Q9JLN6-1]
    RefSeqiNP_001041640.1. NM_001048175.2. [Q9JLN6-3]
    NP_034212.1. NM_010082.2. [Q9JLN6-1]
    NP_899222.1. NM_183366.3. [Q9JLN6-2]
    UniGeneiMm.117450.

    Genome annotation databases

    EnsembliENSMUST00000022642; ENSMUSP00000022642; ENSMUSG00000014725. [Q9JLN6-1]
    ENSMUST00000111072; ENSMUSP00000106701; ENSMUSG00000014725. [Q9JLN6-3]
    GeneIDi13522.
    KEGGimmu:13522.
    UCSCiuc007ult.1. mouse. [Q9JLN6-3]
    uc007ulv.1. mouse. [Q9JLN6-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF153350 mRNA. Translation: AAF71993.1 .
    AF163290 mRNA. Translation: AAM21935.1 .
    AF163291 mRNA. Translation: AAM21936.1 .
    AF163292 mRNA. Translation: AAM21937.1 .
    BC058782 mRNA. Translation: AAH58782.1 .
    CCDSi CCDS36964.1. [Q9JLN6-3 ]
    CCDS36965.1. [Q9JLN6-1 ]
    RefSeqi NP_001041640.1. NM_001048175.2. [Q9JLN6-3 ]
    NP_034212.1. NM_010082.2. [Q9JLN6-1 ]
    NP_899222.1. NM_183366.3. [Q9JLN6-2 ]
    UniGenei Mm.117450.

    3D structure databases

    ProteinModelPortali Q9JLN6.
    SMRi Q9JLN6. Positions 204-623, 633-660.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi M12.020.

    PTM databases

    PhosphoSitei Q9JLN6.

    Proteomic databases

    PaxDbi Q9JLN6.
    PRIDEi Q9JLN6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022642 ; ENSMUSP00000022642 ; ENSMUSG00000014725 . [Q9JLN6-1 ]
    ENSMUST00000111072 ; ENSMUSP00000106701 ; ENSMUSG00000014725 . [Q9JLN6-3 ]
    GeneIDi 13522.
    KEGGi mmu:13522.
    UCSCi uc007ult.1. mouse. [Q9JLN6-3 ]
    uc007ulv.1. mouse. [Q9JLN6-1 ]

    Organism-specific databases

    CTDi 10863.
    MGIi MGI:105988. Adam28.

    Phylogenomic databases

    eggNOGi NOG277164.
    GeneTreei ENSGT00740000114848.
    HOGENOMi HOG000230883.
    HOVERGENi HBG006978.
    InParanoidi Q9JLN6.
    KOi K08614.
    OMAi TSPQIMD.
    OrthoDBi EOG7PGDQ7.
    PhylomeDBi Q9JLN6.
    TreeFami TF314733.

    Miscellaneous databases

    NextBioi 284092.
    PMAP-CutDB Q9JLN6.
    PROi Q9JLN6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9JLN6.
    Bgeei Q9JLN6.
    CleanExi MM_ADAM28.
    Genevestigatori Q9JLN6.

    Family and domain databases

    Gene3Di 3.40.390.10. 1 hit.
    4.10.70.10. 1 hit.
    InterProi IPR006586. ADAM_Cys-rich.
    IPR001762. Blood-coag_inhib_Disintegrin.
    IPR018358. Disintegrin_CS.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR002870. Peptidase_M12B_N.
    [Graphical view ]
    Pfami PF08516. ADAM_CR. 1 hit.
    PF00200. Disintegrin. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    [Graphical view ]
    PRINTSi PR00289. DISINTEGRIN.
    SMARTi SM00608. ACR. 1 hit.
    SM00050. DISIN. 1 hit.
    SM00181. EGF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57552. SSF57552. 1 hit.
    PROSITEi PS50215. ADAM_MEPRO. 1 hit.
    PS00427. DISINTEGRIN_1. 1 hit.
    PS50214. DISINTEGRIN_2. 1 hit.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of ADAM28: evidence for autocatalytic pro-domain removal and for cell surface localization of mature ADAM28."
      Howard L., Maciewicz R.A., Blobel C.P.
      Biochem. J. 348:21-27(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), CHARACTERIZATION, MUTAGENESIS OF GLU-343.
      Tissue: Lung.
    2. "An ADAM family member with expression in thymic epithelial cells and related tissues."
      Haidl I.D., Huber G., Eichmann K.
      Gene 283:163-170(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY.
      Tissue: Thymus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Tissue: Olfactory epithelium.

    Entry informationi

    Entry nameiADA28_MOUSE
    AccessioniPrimary (citable) accession number: Q9JLN6
    Secondary accession number(s): Q5D070, Q8K5D2, Q8K5D3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: February 12, 2003
    Last modified: October 1, 2014
    This is version 124 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3