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Protein

Disintegrin and metalloproteinase domain-containing protein 28

Gene

Adam28

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in organogenesis and organ-specific functions such as thymic T-cell development.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi171Zinc; in inhibited formBy similarity1
Metal bindingi342Zinc; catalyticBy similarity1
Active sitei343PROSITE-ProRule annotation1
Metal bindingi346Zinc; catalyticBy similarity1
Metal bindingi352Zinc; catalyticBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.020.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 28 (EC:3.4.24.-)
Short name:
ADAM 28
Alternative name(s):
Thymic epithelial cell-ADAM
Short name:
TECADAM
Gene namesi
Name:Adam28
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:105988. Adam28.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini196 – 668ExtracellularSequence analysisAdd BLAST473
Transmembranei669 – 689HelicalSequence analysisAdd BLAST21
Topological domaini690 – 793CytoplasmicSequence analysisAdd BLAST104

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi343E → A: Abolishes prodomain removal. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000002913221 – 195By similarityAdd BLAST175
ChainiPRO_0000029133196 – 793Disintegrin and metalloproteinase domain-containing protein 28Add BLAST598

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi91N-linked (GlcNAc...)Sequence analysis1
Glycosylationi272N-linked (GlcNAc...)Sequence analysis1
Glycosylationi277N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi317 ↔ 397By similarity
Disulfide bondi357 ↔ 381By similarity
Disulfide bondi359 ↔ 364By similarity
Disulfide bondi468 ↔ 488By similarity
Glycosylationi531N-linked (GlcNAc...)Sequence analysis1
Glycosylationi551N-linked (GlcNAc...)Sequence analysis1
Glycosylationi605N-linked (GlcNAc...)Sequence analysis1
Glycosylationi631N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi632 ↔ 642By similarity
Disulfide bondi636 ↔ 648By similarity
Disulfide bondi650 ↔ 659By similarity

Post-translational modificationi

Pro-domain removal and maturation may be, at least in part, autocatalytic.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ9JLN6.
PRIDEiQ9JLN6.

PTM databases

iPTMnetiQ9JLN6.
PhosphoSitePlusiQ9JLN6.

Miscellaneous databases

PMAP-CutDBQ9JLN6.

Expressioni

Tissue specificityi

Strong expression in thymic epithelial cells and developmentally related tissues including the trachea, thyroid, lung and stomach, but not in lymphocytes. Expressed at high levels also in epididymis. In contrast with human is not expressed in immature or mature lymphocyte populations of thymocytes, lymph node, spleen, and bone marrow.1 Publication

Developmental stagei

The expression patterns in adult and day 15.5 embryos are similar.

Gene expression databases

BgeeiENSMUSG00000014725.
CleanExiMM_ADAM28.
GenevisibleiQ9JLN6. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022642.

Structurei

3D structure databases

ProteinModelPortaliQ9JLN6.
SMRiQ9JLN6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini206 – 402Peptidase M12BPROSITE-ProRule annotationAdd BLAST197
Domaini410 – 496DisintegrinPROSITE-ProRule annotationAdd BLAST87
Domaini628 – 660EGF-likePROSITE-ProRule annotationAdd BLAST33

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi169 – 176Cysteine switchBy similarity8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi497 – 631Cys-richAdd BLAST135
Compositional biasi760 – 765Poly-Pro6

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
GeneTreeiENSGT00760000118888.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiQ9JLN6.
KOiK08614.
OMAiTSPQIMD.
OrthoDBiEOG091G01NX.
PhylomeDBiQ9JLN6.
TreeFamiTF314733.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q9JLN6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQQWSLLVVS FLLSPVPVSA IKELPKAKKY EVVYPIRLHP LRKRETQEPE
60 70 80 90 100
PKETFETELR YKMTVNGKVA VLYLKKNNKL LAPDYSETYY NSSGNKVTTS
110 120 130 140 150
PQIMDSCYYQ GHIVNEKVSA ASISTCQGLR GYISQGDEKY FIEPLSSENL
160 170 180 190 200
DEQAHALFKD DSNEDQEKSN CGVDDALWLQ GLHQDVALPA TRLIKLNDGM
210 220 230 240 250
VQEPKKYIEY YVVLDNGEFK KYNKNLAEIR KIVLEMANYI NMLYNKLDAH
260 270 280 290 300
VALVGVEIWT DGDKIKITPD ANTTLENFSK WRGNDLLKRK HHDIAQLISS
310 320 330 340 350
TDFSGSTVGL AFMSSMCSPY HSVGIVQDHS NYHLRVAGTM AHEMGHNLGM
360 370 380 390 400
IHDYLSCKCP SEVCVMEQSL RFHMPTDFSS CSRVNYKQFL EEKLSHCLFN
410 420 430 440 450
SPLPSDIIST PVCGNQLLEM NEDCDCGTPK ECTNKCCDAR TCKIKAGFQC
460 470 480 490 500
ALGECCEKCQ LKKPGVVCRA AKDECDLPEV CDGKSSHCPG DRFRVNGSPC
510 520 530 540 550
QNGHGYCLKG KCPTLQQQCM DMWGPGTKVA NTSCYKQNEG GTKYGYCHVE
560 570 580 590 600
NGTHMPCKAK DAMCGKLFCE GGSGDLPWKG LTISFLTCKL FDPEDTSQGV
610 620 630 640 650
DMVANGTKCG TNKVCINAEC VDMEKTYKSA NCSSKCKGHA VCDHELQCQC
660 670 680 690 700
KEGWAPPDCE NSATVFHFSI VVGVLFPLAV IFVVVAIVIQ RQSARRKQRR
710 720 730 740 750
VQRLPSTKDA KLHNQKCRPQ KVKDVQPQEM SQMKKLHVSD LPSEEPEPPP
760 770 780 790
DVLITKPNFP PPPIPVSLTG RAKVPFVKTP HPFSQQIGRV YLK
Length:793
Mass (Da):88,671
Last modified:February 12, 2003 - v3
Checksum:i7715E71456D4403B
GO
Isoform 2 (identifier: Q9JLN6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     769-793: Missing.

Show »
Length:768
Mass (Da):85,833
Checksum:i6786AB68FD631A36
GO
Isoform 3 (identifier: Q9JLN6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     769-771: TGR → DPN
     775-793: Missing.

Show »
Length:774
Mass (Da):86,458
Checksum:i93C42E7C92A20786
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005488769 – 793Missing in isoform 2. 1 PublicationAdd BLAST25
Alternative sequenceiVSP_005489769 – 771TGR → DPN in isoform 3. 3 Publications3
Alternative sequenceiVSP_005490775 – 793Missing in isoform 3. 3 PublicationsAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF153350 mRNA. Translation: AAF71993.1.
AF163290 mRNA. Translation: AAM21935.1.
AF163291 mRNA. Translation: AAM21936.1.
AF163292 mRNA. Translation: AAM21937.1.
BC058782 mRNA. Translation: AAH58782.1.
CCDSiCCDS36964.1. [Q9JLN6-3]
CCDS36965.1. [Q9JLN6-1]
RefSeqiNP_001041640.1. NM_001048175.2. [Q9JLN6-3]
NP_034212.1. NM_010082.2. [Q9JLN6-1]
NP_899222.1. NM_183366.3. [Q9JLN6-2]
UniGeneiMm.117450.

Genome annotation databases

EnsembliENSMUST00000022642; ENSMUSP00000022642; ENSMUSG00000014725. [Q9JLN6-1]
ENSMUST00000111072; ENSMUSP00000106701; ENSMUSG00000014725. [Q9JLN6-3]
GeneIDi13522.
KEGGimmu:13522.
UCSCiuc007ult.2. mouse. [Q9JLN6-3]
uc007ulv.1. mouse. [Q9JLN6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF153350 mRNA. Translation: AAF71993.1.
AF163290 mRNA. Translation: AAM21935.1.
AF163291 mRNA. Translation: AAM21936.1.
AF163292 mRNA. Translation: AAM21937.1.
BC058782 mRNA. Translation: AAH58782.1.
CCDSiCCDS36964.1. [Q9JLN6-3]
CCDS36965.1. [Q9JLN6-1]
RefSeqiNP_001041640.1. NM_001048175.2. [Q9JLN6-3]
NP_034212.1. NM_010082.2. [Q9JLN6-1]
NP_899222.1. NM_183366.3. [Q9JLN6-2]
UniGeneiMm.117450.

3D structure databases

ProteinModelPortaliQ9JLN6.
SMRiQ9JLN6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022642.

Protein family/group databases

MEROPSiM12.020.

PTM databases

iPTMnetiQ9JLN6.
PhosphoSitePlusiQ9JLN6.

Proteomic databases

PaxDbiQ9JLN6.
PRIDEiQ9JLN6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022642; ENSMUSP00000022642; ENSMUSG00000014725. [Q9JLN6-1]
ENSMUST00000111072; ENSMUSP00000106701; ENSMUSG00000014725. [Q9JLN6-3]
GeneIDi13522.
KEGGimmu:13522.
UCSCiuc007ult.2. mouse. [Q9JLN6-3]
uc007ulv.1. mouse. [Q9JLN6-1]

Organism-specific databases

CTDi10863.
MGIiMGI:105988. Adam28.

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
GeneTreeiENSGT00760000118888.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiQ9JLN6.
KOiK08614.
OMAiTSPQIMD.
OrthoDBiEOG091G01NX.
PhylomeDBiQ9JLN6.
TreeFamiTF314733.

Miscellaneous databases

PMAP-CutDBQ9JLN6.
PROiQ9JLN6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000014725.
CleanExiMM_ADAM28.
GenevisibleiQ9JLN6. MM.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADA28_MOUSE
AccessioniPrimary (citable) accession number: Q9JLN6
Secondary accession number(s): Q5D070, Q8K5D2, Q8K5D3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: February 12, 2003
Last modified: November 2, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.