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Q9JLN6

- ADA28_MOUSE

UniProt

Q9JLN6 - ADA28_MOUSE

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Protein

Disintegrin and metalloproteinase domain-containing protein 28

Gene

Adam28

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May play a role in organogenesis and organ-specific functions such as thymic T-cell development.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi171 – 1711Zinc; in inhibited formBy similarity
Metal bindingi342 – 3421Zinc; catalyticBy similarity
Active sitei343 – 3431PROSITE-ProRule annotation
Metal bindingi346 – 3461Zinc; catalyticBy similarity
Metal bindingi352 – 3521Zinc; catalyticBy similarity

GO - Molecular functioni

  1. metalloendopeptidase activity Source: InterPro
  2. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.020.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 28 (EC:3.4.24.-)
Short name:
ADAM 28
Alternative name(s):
Thymic epithelial cell-ADAM
Short name:
TECADAM
Gene namesi
Name:Adam28
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:105988. Adam28.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini196 – 668473ExtracellularSequence AnalysisAdd
BLAST
Transmembranei669 – 68921HelicalSequence AnalysisAdd
BLAST
Topological domaini690 – 793104CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi343 – 3431E → A: Abolishes prodomain removal. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Propeptidei21 – 195175By similarityPRO_0000029132Add
BLAST
Chaini196 – 793598Disintegrin and metalloproteinase domain-containing protein 28PRO_0000029133Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi91 – 911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi272 – 2721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi277 – 2771N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi317 ↔ 397By similarity
Disulfide bondi357 ↔ 381By similarity
Disulfide bondi359 ↔ 364By similarity
Disulfide bondi468 ↔ 488By similarity
Glycosylationi531 – 5311N-linked (GlcNAc...)Sequence Analysis
Glycosylationi551 – 5511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi605 – 6051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi631 – 6311N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi632 ↔ 642By similarity
Disulfide bondi636 ↔ 648By similarity
Disulfide bondi650 ↔ 659By similarity

Post-translational modificationi

Pro-domain removal and maturation may be, at least in part, autocatalytic.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ9JLN6.
PRIDEiQ9JLN6.

PTM databases

PhosphoSiteiQ9JLN6.

Miscellaneous databases

PMAP-CutDBQ9JLN6.

Expressioni

Tissue specificityi

Strong expression in thymic epithelial cells and developmentally related tissues including the trachea, thyroid, lung and stomach, but not in lymphocytes. Expressed at high levels also in epididymis. In contrast with human is not expressed in immature or mature lymphocyte populations of thymocytes, lymph node, spleen, and bone marrow.1 Publication

Developmental stagei

The expression patterns in adult and day 15.5 embryos are similar.

Gene expression databases

BgeeiQ9JLN6.
CleanExiMM_ADAM28.
ExpressionAtlasiQ9JLN6. baseline and differential.
GenevestigatoriQ9JLN6.

Structurei

3D structure databases

ProteinModelPortaliQ9JLN6.
SMRiQ9JLN6. Positions 204-623.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini206 – 402197Peptidase M12BPROSITE-ProRule annotationAdd
BLAST
Domaini410 – 49687DisintegrinPROSITE-ProRule annotationAdd
BLAST
Domaini628 – 66033EGF-likePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi169 – 1768Cysteine switchBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi497 – 631135Cys-richAdd
BLAST
Compositional biasi760 – 7656Poly-Pro

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG277164.
GeneTreeiENSGT00760000118888.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiQ9JLN6.
KOiK08614.
OMAiTSPQIMD.
OrthoDBiEOG7PGDQ7.
PhylomeDBiQ9JLN6.
TreeFamiTF314733.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q9JLN6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQQWSLLVVS FLLSPVPVSA IKELPKAKKY EVVYPIRLHP LRKRETQEPE
60 70 80 90 100
PKETFETELR YKMTVNGKVA VLYLKKNNKL LAPDYSETYY NSSGNKVTTS
110 120 130 140 150
PQIMDSCYYQ GHIVNEKVSA ASISTCQGLR GYISQGDEKY FIEPLSSENL
160 170 180 190 200
DEQAHALFKD DSNEDQEKSN CGVDDALWLQ GLHQDVALPA TRLIKLNDGM
210 220 230 240 250
VQEPKKYIEY YVVLDNGEFK KYNKNLAEIR KIVLEMANYI NMLYNKLDAH
260 270 280 290 300
VALVGVEIWT DGDKIKITPD ANTTLENFSK WRGNDLLKRK HHDIAQLISS
310 320 330 340 350
TDFSGSTVGL AFMSSMCSPY HSVGIVQDHS NYHLRVAGTM AHEMGHNLGM
360 370 380 390 400
IHDYLSCKCP SEVCVMEQSL RFHMPTDFSS CSRVNYKQFL EEKLSHCLFN
410 420 430 440 450
SPLPSDIIST PVCGNQLLEM NEDCDCGTPK ECTNKCCDAR TCKIKAGFQC
460 470 480 490 500
ALGECCEKCQ LKKPGVVCRA AKDECDLPEV CDGKSSHCPG DRFRVNGSPC
510 520 530 540 550
QNGHGYCLKG KCPTLQQQCM DMWGPGTKVA NTSCYKQNEG GTKYGYCHVE
560 570 580 590 600
NGTHMPCKAK DAMCGKLFCE GGSGDLPWKG LTISFLTCKL FDPEDTSQGV
610 620 630 640 650
DMVANGTKCG TNKVCINAEC VDMEKTYKSA NCSSKCKGHA VCDHELQCQC
660 670 680 690 700
KEGWAPPDCE NSATVFHFSI VVGVLFPLAV IFVVVAIVIQ RQSARRKQRR
710 720 730 740 750
VQRLPSTKDA KLHNQKCRPQ KVKDVQPQEM SQMKKLHVSD LPSEEPEPPP
760 770 780 790
DVLITKPNFP PPPIPVSLTG RAKVPFVKTP HPFSQQIGRV YLK
Length:793
Mass (Da):88,671
Last modified:February 12, 2003 - v3
Checksum:i7715E71456D4403B
GO
Isoform 2 (identifier: Q9JLN6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     769-793: Missing.

Show »
Length:768
Mass (Da):85,833
Checksum:i6786AB68FD631A36
GO
Isoform 3 (identifier: Q9JLN6-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     769-771: TGR → DPN
     775-793: Missing.

Show »
Length:774
Mass (Da):86,458
Checksum:i93C42E7C92A20786
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei769 – 79325Missing in isoform 2. 1 PublicationVSP_005488Add
BLAST
Alternative sequencei769 – 7713TGR → DPN in isoform 3. 3 PublicationsVSP_005489
Alternative sequencei775 – 79319Missing in isoform 3. 3 PublicationsVSP_005490Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF153350 mRNA. Translation: AAF71993.1.
AF163290 mRNA. Translation: AAM21935.1.
AF163291 mRNA. Translation: AAM21936.1.
AF163292 mRNA. Translation: AAM21937.1.
BC058782 mRNA. Translation: AAH58782.1.
CCDSiCCDS36964.1. [Q9JLN6-3]
CCDS36965.1. [Q9JLN6-1]
RefSeqiNP_001041640.1. NM_001048175.2. [Q9JLN6-3]
NP_034212.1. NM_010082.2. [Q9JLN6-1]
NP_899222.1. NM_183366.3. [Q9JLN6-2]
UniGeneiMm.117450.

Genome annotation databases

EnsembliENSMUST00000022642; ENSMUSP00000022642; ENSMUSG00000014725. [Q9JLN6-1]
ENSMUST00000111072; ENSMUSP00000106701; ENSMUSG00000014725. [Q9JLN6-3]
GeneIDi13522.
KEGGimmu:13522.
UCSCiuc007ult.1. mouse. [Q9JLN6-3]
uc007ulv.1. mouse. [Q9JLN6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF153350 mRNA. Translation: AAF71993.1 .
AF163290 mRNA. Translation: AAM21935.1 .
AF163291 mRNA. Translation: AAM21936.1 .
AF163292 mRNA. Translation: AAM21937.1 .
BC058782 mRNA. Translation: AAH58782.1 .
CCDSi CCDS36964.1. [Q9JLN6-3 ]
CCDS36965.1. [Q9JLN6-1 ]
RefSeqi NP_001041640.1. NM_001048175.2. [Q9JLN6-3 ]
NP_034212.1. NM_010082.2. [Q9JLN6-1 ]
NP_899222.1. NM_183366.3. [Q9JLN6-2 ]
UniGenei Mm.117450.

3D structure databases

ProteinModelPortali Q9JLN6.
SMRi Q9JLN6. Positions 204-623.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M12.020.

PTM databases

PhosphoSitei Q9JLN6.

Proteomic databases

PaxDbi Q9JLN6.
PRIDEi Q9JLN6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000022642 ; ENSMUSP00000022642 ; ENSMUSG00000014725 . [Q9JLN6-1 ]
ENSMUST00000111072 ; ENSMUSP00000106701 ; ENSMUSG00000014725 . [Q9JLN6-3 ]
GeneIDi 13522.
KEGGi mmu:13522.
UCSCi uc007ult.1. mouse. [Q9JLN6-3 ]
uc007ulv.1. mouse. [Q9JLN6-1 ]

Organism-specific databases

CTDi 10863.
MGIi MGI:105988. Adam28.

Phylogenomic databases

eggNOGi NOG277164.
GeneTreei ENSGT00760000118888.
HOGENOMi HOG000230883.
HOVERGENi HBG006978.
InParanoidi Q9JLN6.
KOi K08614.
OMAi TSPQIMD.
OrthoDBi EOG7PGDQ7.
PhylomeDBi Q9JLN6.
TreeFami TF314733.

Miscellaneous databases

NextBioi 284092.
PMAP-CutDB Q9JLN6.
PROi Q9JLN6.
SOURCEi Search...

Gene expression databases

Bgeei Q9JLN6.
CleanExi MM_ADAM28.
ExpressionAtlasi Q9JLN6. baseline and differential.
Genevestigatori Q9JLN6.

Family and domain databases

Gene3Di 3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProi IPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view ]
Pfami PF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view ]
PRINTSi PR00289. DISINTEGRIN.
SMARTi SM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view ]
SUPFAMi SSF57552. SSF57552. 1 hit.
PROSITEi PS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of ADAM28: evidence for autocatalytic pro-domain removal and for cell surface localization of mature ADAM28."
    Howard L., Maciewicz R.A., Blobel C.P.
    Biochem. J. 348:21-27(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), CHARACTERIZATION, MUTAGENESIS OF GLU-343.
    Tissue: Lung.
  2. "An ADAM family member with expression in thymic epithelial cells and related tissues."
    Haidl I.D., Huber G., Eichmann K.
    Gene 283:163-170(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY.
    Tissue: Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Olfactory epithelium.

Entry informationi

Entry nameiADA28_MOUSE
AccessioniPrimary (citable) accession number: Q9JLN6
Secondary accession number(s): Q5D070, Q8K5D2, Q8K5D3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: February 12, 2003
Last modified: November 26, 2014
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3