Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Growth factor receptor-bound protein 14

Gene

Grb14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal (By similarity).By similarity

GO - Molecular functioni

  • SH3/SH2 adaptor activity Source: MGI

GO - Biological processi

  • signal transduction Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-210993. Tie2 Signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Growth factor receptor-bound protein 14
Alternative name(s):
GRB14 adapter protein
Gene namesi
Name:Grb14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1355324. Grb14.

Subcellular locationi

  • Cytoplasm By similarity
  • Endosome membrane By similarity; Peripheral membrane protein By similarity

  • Note: Upon insulin stimulation, translocates to the plasma membrane.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 538537Growth factor receptor-bound protein 14PRO_0000150349Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineBy similarity
Modified residuei370 – 3701PhosphoserineBy similarity
Modified residuei373 – 3731PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated on serine residues. Phosphorylated on tyrosine residues by TEK/TIE2 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9JLM9.
PaxDbiQ9JLM9.
PRIDEiQ9JLM9.

PTM databases

iPTMnetiQ9JLM9.
PhosphoSiteiQ9JLM9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026888.
CleanExiMM_GRB14.
ExpressionAtlasiQ9JLM9. baseline and differential.
GenevisibleiQ9JLM9. MM.

Interactioni

Subunit structurei

Interacts with the cytoplasmic domain of the autophosphorylated insulin receptor, through the SH2 domain. Interacts with GRB14 (via BPS domain); this interaction protects the tyrosines in the activation loop on INSR from dephosphorylation (By similarity). Binds to the ankyrin repeat region of TNKS2 via its N-terminus. Interacts with activated NRAS (By similarity). Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CNGA1P299732EBI-8347358,EBI-8417095From a different organism.

GO - Molecular functioni

  • SH3/SH2 adaptor activity Source: MGI

Protein-protein interaction databases

BioGridi206154. 1 interaction.
IntActiQ9JLM9. 2 interactions.
MINTiMINT-4096749.
STRINGi10090.ENSMUSP00000028252.

Structurei

3D structure databases

ProteinModelPortaliQ9JLM9.
SMRiQ9JLM9. Positions 104-354, 371-535.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini104 – 19087Ras-associatingPROSITE-ProRule annotationAdd
BLAST
Domaini232 – 340109PHPROSITE-ProRule annotationAdd
BLAST
Domaini437 – 53397SH2PROSITE-ProRule annotationAdd
BLAST

Domaini

The PH domain binds relatively non-specifically and with low affinity to several phosphoinositides, the best binder being PI(3,4,5)P3.By similarity

Sequence similaritiesi

Belongs to the GRB7/10/14 family.Curated
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiENOG410KD2U. Eukaryota.
ENOG410ZN6J. LUCA.
GeneTreeiENSGT00550000074537.
HOGENOMiHOG000231904.
HOVERGENiHBG000468.
InParanoidiQ9JLM9.
OMAiERIIEDH.
OrthoDBiEOG091G135G.
PhylomeDBiQ9JLM9.
TreeFamiTF317511.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR015042. BPS-dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000159. RA_dom.
IPR000980. SH2.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF08947. BPS. 1 hit.
PF00169. PH. 1 hit.
PF00788. RA. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
SMARTiSM00233. PH. 1 hit.
SM00314. RA. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50200. RA. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JLM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSLQDGQS AAGRAGAQDS PLAVQVCRVA QGKGDAQDPA QVPGLHALSP
60 70 80 90 100
ASDATLRGAI DRRKMKDLDV LEKPPIPNPF PELCCSPLTS VLSAGLFPRA
110 120 130 140 150
NSRKKQVIKV YSEDETSRAL EVPSDITARD VCQLLILKNH YVDDNSWTLF
160 170 180 190 200
EHLSHIGLER TVEDHELPTE VLSHWGVEED NKLYLRKNYA KYEFFKNPMY
210 220 230 240 250
FFPEHMVSFA AEMNGDRSPT QILQVFLSSS TYPEIHGFLH AKEQGKKSWK
260 270 280 290 300
KAYFFLRRSG LYFSTKGTSK EPRHLQLFSE FSTSHVYMSL AGKKKHGAPT
310 320 330 340 350
PYGFCLKPNK AGGPRDLKML CAEEEQSRTC WVTAIRLLKD GMQLYQNYMH
360 370 380 390 400
PYQGRSACNS QSMSPMRSVS ENSLVAMDFS GEKSRVIDNP TEALSVAVEE
410 420 430 440 450
GLAWRKKGCL RLGNHGSPSA PSQSSAVNMA LHRSQPWFHH RISRDEAQRL
460 470 480 490 500
IIRQGPVDGV FLVRDSQSNP RTFVLSMSHG QKIKHYQIIP VEDDGELFHT
510 520 530
LDDGHTKFTD LIQLVEFYQL NRGVLPCKLK HYCARMAV
Length:538
Mass (Da):60,573
Last modified:October 1, 2000 - v1
Checksum:i04ABD6CEB6ABC6CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155647 mRNA. Translation: AAF43996.1.
AK010849 mRNA. Translation: BAB27221.3.
AK010903 mRNA. Translation: BAB27256.3.
AK147041 mRNA. Translation: BAE27629.1.
BC021820 mRNA. Translation: AAH21820.1.
CCDSiCCDS16071.1.
RefSeqiNP_057928.1. NM_016719.1.
UniGeneiMm.214554.

Genome annotation databases

EnsembliENSMUST00000028252; ENSMUSP00000028252; ENSMUSG00000026888.
GeneIDi50915.
KEGGimmu:50915.
UCSCiuc008jvx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155647 mRNA. Translation: AAF43996.1.
AK010849 mRNA. Translation: BAB27221.3.
AK010903 mRNA. Translation: BAB27256.3.
AK147041 mRNA. Translation: BAE27629.1.
BC021820 mRNA. Translation: AAH21820.1.
CCDSiCCDS16071.1.
RefSeqiNP_057928.1. NM_016719.1.
UniGeneiMm.214554.

3D structure databases

ProteinModelPortaliQ9JLM9.
SMRiQ9JLM9. Positions 104-354, 371-535.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206154. 1 interaction.
IntActiQ9JLM9. 2 interactions.
MINTiMINT-4096749.
STRINGi10090.ENSMUSP00000028252.

PTM databases

iPTMnetiQ9JLM9.
PhosphoSiteiQ9JLM9.

Proteomic databases

MaxQBiQ9JLM9.
PaxDbiQ9JLM9.
PRIDEiQ9JLM9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028252; ENSMUSP00000028252; ENSMUSG00000026888.
GeneIDi50915.
KEGGimmu:50915.
UCSCiuc008jvx.2. mouse.

Organism-specific databases

CTDi2888.
MGIiMGI:1355324. Grb14.

Phylogenomic databases

eggNOGiENOG410KD2U. Eukaryota.
ENOG410ZN6J. LUCA.
GeneTreeiENSGT00550000074537.
HOGENOMiHOG000231904.
HOVERGENiHBG000468.
InParanoidiQ9JLM9.
OMAiERIIEDH.
OrthoDBiEOG091G135G.
PhylomeDBiQ9JLM9.
TreeFamiTF317511.

Enzyme and pathway databases

ReactomeiR-MMU-210993. Tie2 Signaling.

Miscellaneous databases

PROiQ9JLM9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026888.
CleanExiMM_GRB14.
ExpressionAtlasiQ9JLM9. baseline and differential.
GenevisibleiQ9JLM9. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR015042. BPS-dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000159. RA_dom.
IPR000980. SH2.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF08947. BPS. 1 hit.
PF00169. PH. 1 hit.
PF00788. RA. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
SMARTiSM00233. PH. 1 hit.
SM00314. RA. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50200. RA. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRB14_MOUSE
AccessioniPrimary (citable) accession number: Q9JLM9
Secondary accession number(s): Q3UI77, Q8VDI2, Q9CR03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.