Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Growth factor receptor-bound protein 14

Gene

Grb14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • negative regulation of insulin receptor signaling pathway Source: MGI
  • signal transduction Source: MGI

Enzyme and pathway databases

ReactomeiR-MMU-210993 Tie2 Signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Growth factor receptor-bound protein 14
Alternative name(s):
GRB14 adapter protein
Gene namesi
Name:Grb14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1355324 Grb14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001503492 – 538Growth factor receptor-bound protein 14Add BLAST537

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1
Modified residuei370PhosphoserineBy similarity1
Modified residuei373PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on serine residues. Phosphorylated on tyrosine residues by TEK/TIE2 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9JLM9
PaxDbiQ9JLM9
PRIDEiQ9JLM9

PTM databases

iPTMnetiQ9JLM9
PhosphoSitePlusiQ9JLM9

Expressioni

Gene expression databases

BgeeiENSMUSG00000026888
CleanExiMM_GRB14
ExpressionAtlasiQ9JLM9 baseline and differential
GenevisibleiQ9JLM9 MM

Interactioni

Subunit structurei

Interacts with the cytoplasmic domain of the autophosphorylated insulin receptor, through the SH2 domain. Interacts with GRB14 (via BPS domain); this interaction protects the tyrosines in the activation loop on INSR from dephosphorylation (By similarity). Binds to the ankyrin repeat region of TNKS2 via its N-terminus. Interacts with activated NRAS (By similarity). Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CNGA1P299732EBI-8347358,EBI-8417095From Homo sapiens.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi206154, 1 interactor
IntActiQ9JLM9, 2 interactors
MINTiQ9JLM9
STRINGi10090.ENSMUSP00000028252

Structurei

3D structure databases

ProteinModelPortaliQ9JLM9
SMRiQ9JLM9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 190Ras-associatingPROSITE-ProRule annotationAdd BLAST87
Domaini232 – 340PHPROSITE-ProRule annotationAdd BLAST109
Domaini437 – 533SH2PROSITE-ProRule annotationAdd BLAST97

Domaini

The PH domain binds relatively non-specifically and with low affinity to several phosphoinositides, the best binder being PI(3,4,5)P3.By similarity

Sequence similaritiesi

Belongs to the GRB7/10/14 family.Curated

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiENOG410KD2U Eukaryota
ENOG410ZN6J LUCA
GeneTreeiENSGT00550000074537
HOGENOMiHOG000231904
HOVERGENiHBG000468
InParanoidiQ9JLM9
OMAiQPHQKQK
OrthoDBiEOG091G135G
PhylomeDBiQ9JLM9
TreeFamiTF317511

Family and domain databases

CDDicd10414 SH2_Grb14, 1 hit
Gene3Di2.30.29.30, 1 hit
3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR015042 BPS-dom
IPR035035 Grb14
IPR035034 Grb14_SH2
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR000159 RA_dom
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR11243:SF22 PTHR11243:SF22, 1 hit
PfamiView protein in Pfam
PF08947 BPS, 1 hit
PF00169 PH, 1 hit
PF00788 RA, 1 hit
PF00017 SH2, 1 hit
PRINTSiPR00401 SH2DOMAIN
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00314 RA, 1 hit
SM00252 SH2, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
PS50200 RA, 1 hit
PS50001 SH2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JLM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSLQDGQS AAGRAGAQDS PLAVQVCRVA QGKGDAQDPA QVPGLHALSP
60 70 80 90 100
ASDATLRGAI DRRKMKDLDV LEKPPIPNPF PELCCSPLTS VLSAGLFPRA
110 120 130 140 150
NSRKKQVIKV YSEDETSRAL EVPSDITARD VCQLLILKNH YVDDNSWTLF
160 170 180 190 200
EHLSHIGLER TVEDHELPTE VLSHWGVEED NKLYLRKNYA KYEFFKNPMY
210 220 230 240 250
FFPEHMVSFA AEMNGDRSPT QILQVFLSSS TYPEIHGFLH AKEQGKKSWK
260 270 280 290 300
KAYFFLRRSG LYFSTKGTSK EPRHLQLFSE FSTSHVYMSL AGKKKHGAPT
310 320 330 340 350
PYGFCLKPNK AGGPRDLKML CAEEEQSRTC WVTAIRLLKD GMQLYQNYMH
360 370 380 390 400
PYQGRSACNS QSMSPMRSVS ENSLVAMDFS GEKSRVIDNP TEALSVAVEE
410 420 430 440 450
GLAWRKKGCL RLGNHGSPSA PSQSSAVNMA LHRSQPWFHH RISRDEAQRL
460 470 480 490 500
IIRQGPVDGV FLVRDSQSNP RTFVLSMSHG QKIKHYQIIP VEDDGELFHT
510 520 530
LDDGHTKFTD LIQLVEFYQL NRGVLPCKLK HYCARMAV
Length:538
Mass (Da):60,573
Last modified:October 1, 2000 - v1
Checksum:i04ABD6CEB6ABC6CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155647 mRNA Translation: AAF43996.1
AK010849 mRNA Translation: BAB27221.3
AK010903 mRNA Translation: BAB27256.3
AK147041 mRNA Translation: BAE27629.1
BC021820 mRNA Translation: AAH21820.1
CCDSiCCDS16071.1
RefSeqiNP_057928.1, NM_016719.1
UniGeneiMm.214554

Genome annotation databases

EnsembliENSMUST00000028252; ENSMUSP00000028252; ENSMUSG00000026888
GeneIDi50915
KEGGimmu:50915
UCSCiuc008jvx.2 mouse

Similar proteinsi

Entry informationi

Entry nameiGRB14_MOUSE
AccessioniPrimary (citable) accession number: Q9JLM9
Secondary accession number(s): Q3UI77, Q8VDI2, Q9CR03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: October 1, 2000
Last modified: March 28, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health