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Q9JLM8 (DCLK1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase DCLK1
EC=2.7.11.1
Alternative name(s):
Doublecortin-like and CAM kinase-like 1
Doublecortin-like kinase 1
Gene names
Name:Dclk1
Synonyms:Dcamkl1, Dclk
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length756 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

Contains 2 doublecortin domains.

Contains 1 protein kinase domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9JLM8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9JLM8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     343-363: KQRISQHGGSSTSLSSTKVCS → RQRDLYRPLSSDDLDSVGDSV
     364-756: Missing.
Note: Contains a phosphoserine at position 352.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 756756Serine/threonine-protein kinase DCLK1
PRO_0000085920

Regions

Domain57 – 14387Doublecortin 1
Domain186 – 26984Doublecortin 2
Domain406 – 663258Protein kinase
Nucleotide binding412 – 4209ATP By similarity
Compositional bias298 – 35861Pro/Ser-rich

Sites

Active site5271Proton acceptor By similarity
Binding site4351ATP By similarity

Amino acid modifications

Modified residue321Phosphoserine Ref.7
Modified residue361Phosphoserine Ref.7
Modified residue3051Phosphoserine Ref.3
Modified residue3071Phosphoserine Ref.3 Ref.6
Modified residue3301Phosphoserine Ref.8
Modified residue3321Phosphoserine Ref.6 Ref.8
Modified residue3361Phosphothreonine Ref.6
Modified residue3371Phosphoserine Ref.8
Modified residue3521Phosphoserine By similarity
Modified residue3641Phosphoserine Ref.4
Modified residue3921Phosphoserine Ref.4
Modified residue5091Phosphotyrosine Ref.5
Modified residue5361Phosphotyrosine Ref.5
Modified residue7381Phosphoserine Ref.3
Modified residue7421Phosphoserine Ref.3

Natural variations

Alternative sequence343 – 36321KQRIS…TKVCS → RQRDLYRPLSSDDLDSVGDS V in isoform 2.
VSP_019593
Alternative sequence364 – 756393Missing in isoform 2.
VSP_019594

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 3D1DBF18C23129F2

FASTA75684,153
        10         20         30         40         50         60 
MSFGRDMELE HFDERDKAQR YSRGSRVNGL PSPTHSAHCS FYRTRTLQTL SSEKKAKKVR 

        70         80         90        100        110        120 
FYRNGDRYFK GIVYAISPDR FRSFEALLAD LTRTLSDNVN LPQGVRTIYT IDGLKKISSL 

       130        140        150        160        170        180 
DQLVEGESYV CGSIEPFKKL EYTKNVNPNW SVNVKTTSAS RAVSSLATAK GGPSEVRENK 

       190        200        210        220        230        240 
DFIRPKLVTI IRSGVKPRKA VRILLNKKTA HSFEQVLTDI TDAIKLDSGV VKRLYTLDGK 

       250        260        270        280        290        300 
QVMCLQDFFG DDDIFIACGP EKFRYQDDFL LDESECRVVK STSYTKIASA SRRGTTKSPG 

       310        320        330        340        350        360 
PSRRSKSPAS TSSVNGTPGS QLSTPRSGKS PSPSPTSPGS LRKQRISQHG GSSTSLSSTK 

       370        380        390        400        410        420 
VCSSMDENDG PGEGDELGRR HSLQRGWRRE ESEEGFQIPA TITERYKVGR TIGDGNFAVV 

       430        440        450        460        470        480 
KECIERSTAR EYALKIIKKS KCRGKEHMIQ NEVSILRRVK HPNIVLLIEE MDVPTELYLV 

       490        500        510        520        530        540 
MELVKGGDLF DAITSTSKYT ERDASGMLYN LASAIKYLHS LNIVHRDIKP ENLLVYEHQD 

       550        560        570        580        590        600 
GSKSLKLGDF GLATIVDGPL YTVCGTPTYV APEIIAETGY GLKVDIWAAG VITYILLCGF 

       610        620        630        640        650        660 
PPFRGSGDDQ EVLFDQILMG QVDFPSPYWD NVSDSAKELI NMMLLVNVDQ RFSAVQVLEH 

       670        680        690        700        710        720 
PWVNDDGLPE NEHQLSVAGK IKKHFNTGPK PSSTAAGVSV IATTALDKER QVFRRRRNQD 

       730        740        750 
VRSRYKAQPA PPELNSESED YSPSSSETVR SPNSPF

« Hide

Isoform 2 [UniParc].

Checksum: 66EC3E5C6BF46993
Show »

FASTA36340,409

References

« Hide 'large scale' references
[1]"KIAA0369, doublecortin-like kinase, is expressed during brain development."
Burgess H.A., Martinez S., Reiner O.
J. Neurosci. Res. 58:567-575(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM1).
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Embryo.
[3]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-305; SER-307; SER-738 AND SER-742, MASS SPECTROMETRY.
Tissue: Embryonic brain.
[4]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-364 AND SER-392, MASS SPECTROMETRY.
Tissue: Brain.
[5]"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-509 AND TYR-536, MASS SPECTROMETRY.
Tissue: Brain.
[6]"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-307; SER-332 AND THR-336, MASS SPECTROMETRY.
Tissue: Brain cortex.
[7]"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry."
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M.
J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32 AND SER-36, MASS SPECTROMETRY.
Tissue: Melanoma.
[8]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-330; SER-332 AND SER-337, MASS SPECTROMETRY.
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF155819 mRNA. Translation: AAF26673.1.
BC064783 mRNA. Translation: AAH64783.1.
IPIIPI00468380.
IPI00761729.
RefSeqNP_064362.1. NM_019978.3.
UniGeneMm.393242.
Mm.472264.

3D structure databases

ProteinModelPortalQ9JLM8.
SMRQ9JLM8. Positions 54-154, 172-264, 400-733.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9JLM8. 3 interactions.
STRING10090.ENSMUSP00000050034.

PTM databases

PhosphoSiteQ9JLM8.

Proteomic databases

PaxDbQ9JLM8.
PRIDEQ9JLM8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000054237; ENSMUSP00000050034; ENSMUSG00000027797.
GeneID13175.
KEGGmmu:13175.
UCSCuc008pgl.2. mouse.

Organism-specific databases

CTD9201.
MGIMGI:1330861. Dclk1.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00680000099865.
HOGENOMHOG000230855.
HOVERGENHBG003790.
InParanoidQ9JLM8.
KOK08805.
OMAFLLDESX.
OrthoDBEOG4H9XK1.

Enzyme and pathway databases

BRENDA2.7.11.1. 3474.

Gene expression databases

ArrayExpressQ9JLM8.
BgeeQ9JLM8.
CleanExMM_DCLK1.
GenevestigatorQ9JLM8.
GermOnlineENSMUSG00000027797. Mus musculus.

Family and domain databases

Gene3D3.10.20.230. 2 hits.
InterProIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR003533. Doublecortin_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERPTHR24347. PTHR24347. 1 hit.
PfamPF03607. DCX. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00537. DCX. 2 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF89837. Doublecortin_dom. 2 hits.
SSF56112. Kinase_like. 1 hit.
PROSITEPS50309. DC. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDCLK1. mouse.
NextBio283276.
PMAP-CutDBQ9JLM8.
SOURCESearch...

Entry information

Entry nameDCLK1_MOUSE
AccessionPrimary (citable) accession number: Q9JLM8
Secondary accession number(s): Q6P207
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: October 1, 2000
Last modified: May 1, 2013
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents