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Q9JLM8

- DCLK1_MOUSE

UniProt

Q9JLM8 - DCLK1_MOUSE

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Protein

Serine/threonine-protein kinase DCLK1

Gene

Dclk1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei435 – 4351ATPPROSITE-ProRule annotation
Active sitei527 – 5271Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi412 – 4209ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. axon extension Source: MGI
  2. axonogenesis Source: MGI
  3. brain development Source: MGI
  4. central nervous system projection neuron axonogenesis Source: MGI
  5. dendrite morphogenesis Source: MGI
  6. forebrain development Source: MGI
  7. intracellular signal transduction Source: InterPro
  8. neuron migration Source: MGI
  9. response to virus Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation, Neurogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase DCLK1 (EC:2.7.11.1)
Alternative name(s):
Doublecortin-like and CAM kinase-like 1
Doublecortin-like kinase 1
Gene namesi
Name:Dclk1
Synonyms:Dcamkl1, Dclk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:1330861. Dclk1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 756756Serine/threonine-protein kinase DCLK1PRO_0000085920Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei307 – 3071Phosphoserine1 Publication
Modified residuei330 – 3301Phosphoserine1 Publication
Modified residuei332 – 3321Phosphoserine1 Publication
Modified residuei337 – 3371Phosphoserine1 Publication
Modified residuei352 – 3521PhosphoserineBy similarity
Modified residuei392 – 3921Phosphoserine1 Publication
Modified residuei536 – 5361Phosphotyrosine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9JLM8.
PaxDbiQ9JLM8.
PRIDEiQ9JLM8.

PTM databases

PhosphoSiteiQ9JLM8.

Miscellaneous databases

PMAP-CutDBQ9JLM8.

Expressioni

Gene expression databases

BgeeiQ9JLM8.
CleanExiMM_DCLK1.
ExpressionAtlasiQ9JLM8. baseline and differential.
GenevestigatoriQ9JLM8.

Interactioni

Protein-protein interaction databases

BioGridi199063. 4 interactions.
IntActiQ9JLM8. 3 interactions.
STRINGi10090.ENSMUSP00000050034.

Structurei

3D structure databases

ProteinModelPortaliQ9JLM8.
SMRiQ9JLM8. Positions 54-154, 402-727.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini57 – 14387Doublecortin 1PROSITE-ProRule annotationAdd
BLAST
Domaini186 – 26984Doublecortin 2PROSITE-ProRule annotationAdd
BLAST
Domaini406 – 663258Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi298 – 35861Pro/Ser-richAdd
BLAST

Sequence similaritiesi

Contains 2 doublecortin domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000119185.
HOGENOMiHOG000230855.
HOVERGENiHBG003790.
InParanoidiQ9JLM8.
KOiK08805.
OMAiFLLDESX.
OrthoDBiEOG7M98FQ.
PhylomeDBiQ9JLM8.
TreeFamiTF318770.

Family and domain databases

Gene3Di3.10.20.230. 2 hits.
InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR003533. Doublecortin_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF03607. DCX. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00537. DCX. 2 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50309. DC. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9JLM8) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSFGRDMELE HFDERDKAQR YSRGSRVNGL PSPTHSAHCS FYRTRTLQTL
60 70 80 90 100
SSEKKAKKVR FYRNGDRYFK GIVYAISPDR FRSFEALLAD LTRTLSDNVN
110 120 130 140 150
LPQGVRTIYT IDGLKKISSL DQLVEGESYV CGSIEPFKKL EYTKNVNPNW
160 170 180 190 200
SVNVKTTSAS RAVSSLATAK GGPSEVRENK DFIRPKLVTI IRSGVKPRKA
210 220 230 240 250
VRILLNKKTA HSFEQVLTDI TDAIKLDSGV VKRLYTLDGK QVMCLQDFFG
260 270 280 290 300
DDDIFIACGP EKFRYQDDFL LDESECRVVK STSYTKIASA SRRGTTKSPG
310 320 330 340 350
PSRRSKSPAS TSSVNGTPGS QLSTPRSGKS PSPSPTSPGS LRKQRISQHG
360 370 380 390 400
GSSTSLSSTK VCSSMDENDG PGEGDELGRR HSLQRGWRRE ESEEGFQIPA
410 420 430 440 450
TITERYKVGR TIGDGNFAVV KECIERSTAR EYALKIIKKS KCRGKEHMIQ
460 470 480 490 500
NEVSILRRVK HPNIVLLIEE MDVPTELYLV MELVKGGDLF DAITSTSKYT
510 520 530 540 550
ERDASGMLYN LASAIKYLHS LNIVHRDIKP ENLLVYEHQD GSKSLKLGDF
560 570 580 590 600
GLATIVDGPL YTVCGTPTYV APEIIAETGY GLKVDIWAAG VITYILLCGF
610 620 630 640 650
PPFRGSGDDQ EVLFDQILMG QVDFPSPYWD NVSDSAKELI NMMLLVNVDQ
660 670 680 690 700
RFSAVQVLEH PWVNDDGLPE NEHQLSVAGK IKKHFNTGPK PSSTAAGVSV
710 720 730 740 750
IATTALDKER QVFRRRRNQD VRSRYKAQPA PPELNSESED YSPSSSETVR

SPNSPF
Length:756
Mass (Da):84,153
Last modified:October 1, 2000 - v1
Checksum:i3D1DBF18C23129F2
GO
Isoform 2 (identifier: Q9JLM8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     343-363: KQRISQHGGSSTSLSSTKVCS → RQRDLYRPLSSDDLDSVGDSV
     364-756: Missing.

Show »
Length:363
Mass (Da):40,409
Checksum:i66EC3E5C6BF46993
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei343 – 36321KQRIS…TKVCS → RQRDLYRPLSSDDLDSVGDS V in isoform 2. 1 PublicationVSP_019593Add
BLAST
Alternative sequencei364 – 756393Missing in isoform 2. 1 PublicationVSP_019594Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF155819 mRNA. Translation: AAF26673.1.
BC064783 mRNA. Translation: AAH64783.1.
CCDSiCCDS17359.1. [Q9JLM8-1]
CCDS50909.1. [Q9JLM8-2]
RefSeqiNP_064362.1. NM_019978.3. [Q9JLM8-1]
UniGeneiMm.393242.
Mm.472264.

Genome annotation databases

EnsembliENSMUST00000054237; ENSMUSP00000050034; ENSMUSG00000027797. [Q9JLM8-1]
GeneIDi13175.
KEGGimmu:13175.
UCSCiuc008pgl.2. mouse. [Q9JLM8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF155819 mRNA. Translation: AAF26673.1 .
BC064783 mRNA. Translation: AAH64783.1 .
CCDSi CCDS17359.1. [Q9JLM8-1 ]
CCDS50909.1. [Q9JLM8-2 ]
RefSeqi NP_064362.1. NM_019978.3. [Q9JLM8-1 ]
UniGenei Mm.393242.
Mm.472264.

3D structure databases

ProteinModelPortali Q9JLM8.
SMRi Q9JLM8. Positions 54-154, 402-727.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199063. 4 interactions.
IntActi Q9JLM8. 3 interactions.
STRINGi 10090.ENSMUSP00000050034.

PTM databases

PhosphoSitei Q9JLM8.

Proteomic databases

MaxQBi Q9JLM8.
PaxDbi Q9JLM8.
PRIDEi Q9JLM8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000054237 ; ENSMUSP00000050034 ; ENSMUSG00000027797 . [Q9JLM8-1 ]
GeneIDi 13175.
KEGGi mmu:13175.
UCSCi uc008pgl.2. mouse. [Q9JLM8-1 ]

Organism-specific databases

CTDi 9201.
MGIi MGI:1330861. Dclk1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000119185.
HOGENOMi HOG000230855.
HOVERGENi HBG003790.
InParanoidi Q9JLM8.
KOi K08805.
OMAi FLLDESX.
OrthoDBi EOG7M98FQ.
PhylomeDBi Q9JLM8.
TreeFami TF318770.

Enzyme and pathway databases

BRENDAi 2.7.11.1. 3474.

Miscellaneous databases

ChiTaRSi DCLK1. mouse.
NextBioi 283276.
PMAP-CutDB Q9JLM8.
PROi Q9JLM8.
SOURCEi Search...

Gene expression databases

Bgeei Q9JLM8.
CleanExi MM_DCLK1.
ExpressionAtlasi Q9JLM8. baseline and differential.
Genevestigatori Q9JLM8.

Family and domain databases

Gene3Di 3.10.20.230. 2 hits.
InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR003533. Doublecortin_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR24347. PTHR24347. 1 hit.
Pfami PF03607. DCX. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00537. DCX. 2 hits.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50309. DC. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "KIAA0369, doublecortin-like kinase, is expressed during brain development."
    Burgess H.A., Martinez S., Reiner O.
    J. Neurosci. Res. 58:567-575(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM1).
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Embryo.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-307, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-392, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  5. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-536, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  6. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-330; SER-332 AND SER-337, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiDCLK1_MOUSE
AccessioniPrimary (citable) accession number: Q9JLM8
Secondary accession number(s): Q6P207
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3