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Q9JLM8

- DCLK1_MOUSE

UniProt

Q9JLM8 - DCLK1_MOUSE

Protein

Serine/threonine-protein kinase DCLK1

Gene

Dclk1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei435 – 4351ATPPROSITE-ProRule annotation
    Active sitei527 – 5271Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi412 – 4209ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein serine/threonine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. axon extension Source: MGI
    2. axonogenesis Source: MGI
    3. brain development Source: MGI
    4. central nervous system projection neuron axonogenesis Source: MGI
    5. dendrite morphogenesis Source: MGI
    6. forebrain development Source: MGI
    7. intracellular signal transduction Source: InterPro
    8. neuron migration Source: MGI
    9. response to virus Source: Ensembl

    Keywords - Molecular functioni

    Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Differentiation, Neurogenesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.1. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase DCLK1 (EC:2.7.11.1)
    Alternative name(s):
    Doublecortin-like and CAM kinase-like 1
    Doublecortin-like kinase 1
    Gene namesi
    Name:Dclk1
    Synonyms:Dcamkl1, Dclk
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:1330861. Dclk1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 756756Serine/threonine-protein kinase DCLK1PRO_0000085920Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei307 – 3071Phosphoserine1 Publication
    Modified residuei330 – 3301Phosphoserine1 Publication
    Modified residuei332 – 3321Phosphoserine1 Publication
    Modified residuei337 – 3371Phosphoserine1 Publication
    Modified residuei352 – 3521PhosphoserineBy similarity
    Modified residuei392 – 3921Phosphoserine1 Publication
    Modified residuei536 – 5361Phosphotyrosine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9JLM8.
    PaxDbiQ9JLM8.
    PRIDEiQ9JLM8.

    PTM databases

    PhosphoSiteiQ9JLM8.

    Miscellaneous databases

    PMAP-CutDBQ9JLM8.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9JLM8.
    BgeeiQ9JLM8.
    CleanExiMM_DCLK1.
    GenevestigatoriQ9JLM8.

    Interactioni

    Protein-protein interaction databases

    BioGridi199063. 4 interactions.
    IntActiQ9JLM8. 3 interactions.
    STRINGi10090.ENSMUSP00000050034.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JLM8.
    SMRiQ9JLM8. Positions 54-154, 402-727.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini57 – 14387Doublecortin 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini186 – 26984Doublecortin 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini406 – 663258Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi298 – 35861Pro/Ser-richAdd
    BLAST

    Sequence similaritiesi

    Contains 2 doublecortin domains.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00680000099865.
    HOGENOMiHOG000230855.
    HOVERGENiHBG003790.
    InParanoidiQ9JLM8.
    KOiK08805.
    OMAiFLLDESX.
    OrthoDBiEOG7M98FQ.
    PhylomeDBiQ9JLM8.
    TreeFamiTF318770.

    Family and domain databases

    Gene3Di3.10.20.230. 2 hits.
    InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR003533. Doublecortin_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24347. PTHR24347. 1 hit.
    PfamiPF03607. DCX. 2 hits.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00537. DCX. 2 hits.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50309. DC. 2 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9JLM8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSFGRDMELE HFDERDKAQR YSRGSRVNGL PSPTHSAHCS FYRTRTLQTL    50
    SSEKKAKKVR FYRNGDRYFK GIVYAISPDR FRSFEALLAD LTRTLSDNVN 100
    LPQGVRTIYT IDGLKKISSL DQLVEGESYV CGSIEPFKKL EYTKNVNPNW 150
    SVNVKTTSAS RAVSSLATAK GGPSEVRENK DFIRPKLVTI IRSGVKPRKA 200
    VRILLNKKTA HSFEQVLTDI TDAIKLDSGV VKRLYTLDGK QVMCLQDFFG 250
    DDDIFIACGP EKFRYQDDFL LDESECRVVK STSYTKIASA SRRGTTKSPG 300
    PSRRSKSPAS TSSVNGTPGS QLSTPRSGKS PSPSPTSPGS LRKQRISQHG 350
    GSSTSLSSTK VCSSMDENDG PGEGDELGRR HSLQRGWRRE ESEEGFQIPA 400
    TITERYKVGR TIGDGNFAVV KECIERSTAR EYALKIIKKS KCRGKEHMIQ 450
    NEVSILRRVK HPNIVLLIEE MDVPTELYLV MELVKGGDLF DAITSTSKYT 500
    ERDASGMLYN LASAIKYLHS LNIVHRDIKP ENLLVYEHQD GSKSLKLGDF 550
    GLATIVDGPL YTVCGTPTYV APEIIAETGY GLKVDIWAAG VITYILLCGF 600
    PPFRGSGDDQ EVLFDQILMG QVDFPSPYWD NVSDSAKELI NMMLLVNVDQ 650
    RFSAVQVLEH PWVNDDGLPE NEHQLSVAGK IKKHFNTGPK PSSTAAGVSV 700
    IATTALDKER QVFRRRRNQD VRSRYKAQPA PPELNSESED YSPSSSETVR 750
    SPNSPF 756
    Length:756
    Mass (Da):84,153
    Last modified:October 1, 2000 - v1
    Checksum:i3D1DBF18C23129F2
    GO
    Isoform 2 (identifier: Q9JLM8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         343-363: KQRISQHGGSSTSLSSTKVCS → RQRDLYRPLSSDDLDSVGDSV
         364-756: Missing.

    Show »
    Length:363
    Mass (Da):40,409
    Checksum:i66EC3E5C6BF46993
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei343 – 36321KQRIS…TKVCS → RQRDLYRPLSSDDLDSVGDS V in isoform 2. 1 PublicationVSP_019593Add
    BLAST
    Alternative sequencei364 – 756393Missing in isoform 2. 1 PublicationVSP_019594Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF155819 mRNA. Translation: AAF26673.1.
    BC064783 mRNA. Translation: AAH64783.1.
    CCDSiCCDS17359.1. [Q9JLM8-1]
    CCDS50909.1. [Q9JLM8-2]
    RefSeqiNP_064362.1. NM_019978.3. [Q9JLM8-1]
    UniGeneiMm.393242.
    Mm.472264.

    Genome annotation databases

    EnsembliENSMUST00000054237; ENSMUSP00000050034; ENSMUSG00000027797. [Q9JLM8-1]
    GeneIDi13175.
    KEGGimmu:13175.
    UCSCiuc008pgl.2. mouse. [Q9JLM8-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF155819 mRNA. Translation: AAF26673.1 .
    BC064783 mRNA. Translation: AAH64783.1 .
    CCDSi CCDS17359.1. [Q9JLM8-1 ]
    CCDS50909.1. [Q9JLM8-2 ]
    RefSeqi NP_064362.1. NM_019978.3. [Q9JLM8-1 ]
    UniGenei Mm.393242.
    Mm.472264.

    3D structure databases

    ProteinModelPortali Q9JLM8.
    SMRi Q9JLM8. Positions 54-154, 402-727.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199063. 4 interactions.
    IntActi Q9JLM8. 3 interactions.
    STRINGi 10090.ENSMUSP00000050034.

    PTM databases

    PhosphoSitei Q9JLM8.

    Proteomic databases

    MaxQBi Q9JLM8.
    PaxDbi Q9JLM8.
    PRIDEi Q9JLM8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000054237 ; ENSMUSP00000050034 ; ENSMUSG00000027797 . [Q9JLM8-1 ]
    GeneIDi 13175.
    KEGGi mmu:13175.
    UCSCi uc008pgl.2. mouse. [Q9JLM8-1 ]

    Organism-specific databases

    CTDi 9201.
    MGIi MGI:1330861. Dclk1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00680000099865.
    HOGENOMi HOG000230855.
    HOVERGENi HBG003790.
    InParanoidi Q9JLM8.
    KOi K08805.
    OMAi FLLDESX.
    OrthoDBi EOG7M98FQ.
    PhylomeDBi Q9JLM8.
    TreeFami TF318770.

    Enzyme and pathway databases

    BRENDAi 2.7.11.1. 3474.

    Miscellaneous databases

    ChiTaRSi DCLK1. mouse.
    NextBioi 283276.
    PMAP-CutDB Q9JLM8.
    PROi Q9JLM8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9JLM8.
    Bgeei Q9JLM8.
    CleanExi MM_DCLK1.
    Genevestigatori Q9JLM8.

    Family and domain databases

    Gene3Di 3.10.20.230. 2 hits.
    InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR003533. Doublecortin_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24347. PTHR24347. 1 hit.
    Pfami PF03607. DCX. 2 hits.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00537. DCX. 2 hits.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50309. DC. 2 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "KIAA0369, doublecortin-like kinase, is expressed during brain development."
      Burgess H.A., Martinez S., Reiner O.
      J. Neurosci. Res. 58:567-575(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM1).
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Embryo.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-307, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.
    4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-392, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    5. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-536, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    6. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-330; SER-332 AND SER-337, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiDCLK1_MOUSE
    AccessioniPrimary (citable) accession number: Q9JLM8
    Secondary accession number(s): Q6P207
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2002
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3