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Protein

Calcium-binding protein 1

Gene

Cabp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Modulates calcium-dependent activity of inositol 1,4,5-triphosphate receptors (ITPRs). Inhibits agonist-induced intracellular calcium signaling. Enhances inactivation and does not support calcium-dependent facilitation of voltage-dependent P/Q-type calcium channels. Causes calcium-dependent facilitation and inhibits inactivation of L-type calcium channels by binding to the same sites as calmodulin in the C-terminal domain of CACNA1C, but resulting in an opposit effects on channel function. Suppresses the calcium-dependent inactivation of CACNA1D. Inhibits TRPC5 channels. Prevents NMDA receptor-induced cellular degeneration (By similarity).By similarity2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi95 – 951MagnesiumBy similarity
Metal bindingi97 – 971MagnesiumBy similarity
Metal bindingi99 – 991MagnesiumBy similarity
Metal bindingi101 – 1011Magnesium; via carbonyl oxygenBy similarity
Metal bindingi172 – 1721Calcium 1By similarity
Metal bindingi174 – 1741Calcium 1By similarity
Metal bindingi176 – 1761Calcium 1By similarity
Metal bindingi178 – 1781Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi183 – 1831Calcium 1By similarity
Metal bindingi209 – 2091Calcium 2By similarity
Metal bindingi210 – 2101Calcium 2; via amide nitrogenBy similarity
Metal bindingi211 – 2111Calcium 2By similarity
Metal bindingi213 – 2131Calcium 2By similarity
Metal bindingi214 – 2141Calcium 2; via amide nitrogenBy similarity
Metal bindingi215 – 2151Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi217 – 2171Calcium 2By similarity
Metal bindingi220 – 2201Calcium 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi95 – 106121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi172 – 183122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi209 – 220123PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-binding protein 1
Short name:
CaBP1
Gene namesi
Name:Cabp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1352750. Cabp1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 227226Calcium-binding protein 1PRO_0000073514Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineCurated
Modified residuei3 – 31Deamidated asparagineCurated
Lipidationi4 – 41S-palmitoyl cysteineCurated
Modified residuei180 – 1801PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated. The phosphorylation regulates the activity (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiQ9JLK7.
PaxDbiQ9JLK7.
PRIDEiQ9JLK7.

PTM databases

PhosphoSiteiQ9JLK7.

Expressioni

Gene expression databases

BgeeiQ9JLK7.
CleanExiMM_CABP1.
ExpressionAtlasiQ9JLK7. baseline and differential.
GenevisibleiQ9JLK7. MM.

Interactioni

Subunit structurei

Homodimer; when bound to calcium or magnesium. Interacts (via C-terminus) with ITPR1, ITPR2 and ITPR3. This binding is calcium dependent and the interaction correlates with calcium concentration. An additional calcium-independent interaction with the N-terminus of ITPR1 results in a decreased InsP3 binding to the receptor (By similarity). Interacts with CACNA1A (via C-terminal CDB motif) in the pre- and postsynaptic membranes (By similarity). Interacts with CACNA1D and CACNA1C (via C-terminal C and IQ motifs). The binding to the C motif is calcium independent whereas the binding to IQ requires the presence of calcium and is mutually exclusive with calmodulin binding (By similarity). Interacts with TRPC5 (via C-terminus). Interacts (via EF-hands 1 and 2) at microtubules with MAP1LC3B (By similarity). Interacts with MYO1C. Interacts (via EF-hands 1 and 2) with NSMF (via the central NLS-containing motif region), the interaction occurs in a calcium dependent manner after synaptic NMDA receptor stimulation and prevents nuclear import of NSMF. Interacts with C9orf9 homolog.By similarity5 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi205933. 1 interaction.
STRINGi10090.ENSMUSP00000031519.

Structurei

3D structure databases

ProteinModelPortaliQ9JLK7.
SMRiQ9JLK7. Positions 76-227.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini82 – 11736EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini118 – 15336EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini159 – 19436EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini196 – 22732EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Domaini

EF-1 binds magnesium constitutively under physiological conditions, EF-3 and EF-4 bind calcium cooperatively and EF-2 binds neither calcium nor magnesium.By similarity

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118901.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiQ9JLK7.
OMAiVLAQNCA.
OrthoDBiEOG7XM303.
PhylomeDBiQ9JLK7.
TreeFamiTF334804.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform L-CaBP1 (identifier: Q9JLK7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNCVKSPLR NLSRKMRQEE KTSYMAVQTS EDGLADGGEL HGPLMMLAQN
60 70 80 90 100
CAVMHNLLGP ACIFLRKGFA ENRQPDRSLR PEEIEELREA FREFDKDKDG
110 120 130 140 150
YINCRDLGNC MRTMGYMPTE MELIELSQQI NMNLGGHVDF DDFVELMGPK
160 170 180 190 200
LLAETADMIG VKELRDAFRE FDTNGDGEIS TSELREAMRK LLGHQVGHRD
210 220
IEEIIRDVDL NGDGRVDFEE FVRMMSR
Length:227
Mass (Da):25,943
Last modified:January 23, 2007 - v3
Checksum:iC0AA48F481664617
GO
Isoform S-CaBP1 (identifier: Q9JLK7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     16-75: Missing.

Show »
Length:167
Mass (Da):19,354
Checksum:i3C65D1F2C4338AF3
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei16 – 7560Missing in isoform S-CaBP1. 1 PublicationVSP_000733Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF169152 mRNA. Translation: AAF25786.1.
AF169153 mRNA. Translation: AAF25787.1.
CCDSiCCDS19582.1. [Q9JLK7-1]
CCDS80378.1. [Q9JLK7-2]
RefSeqiNP_001297644.1. NM_001310715.1. [Q9JLK7-2]
NP_038907.1. NM_013879.2. [Q9JLK7-1]
UniGeneiMm.38551.

Genome annotation databases

EnsembliENSMUST00000031519; ENSMUSP00000031519; ENSMUSG00000029544. [Q9JLK7-1]
ENSMUST00000112112; ENSMUSP00000107740; ENSMUSG00000029544. [Q9JLK7-2]
GeneIDi29867.
KEGGimmu:29867.
UCSCiuc008zdg.1. mouse. [Q9JLK7-1]
uc008zdh.1. mouse. [Q9JLK7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF169152 mRNA. Translation: AAF25786.1.
AF169153 mRNA. Translation: AAF25787.1.
CCDSiCCDS19582.1. [Q9JLK7-1]
CCDS80378.1. [Q9JLK7-2]
RefSeqiNP_001297644.1. NM_001310715.1. [Q9JLK7-2]
NP_038907.1. NM_013879.2. [Q9JLK7-1]
UniGeneiMm.38551.

3D structure databases

ProteinModelPortaliQ9JLK7.
SMRiQ9JLK7. Positions 76-227.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205933. 1 interaction.
STRINGi10090.ENSMUSP00000031519.

PTM databases

PhosphoSiteiQ9JLK7.

Proteomic databases

MaxQBiQ9JLK7.
PaxDbiQ9JLK7.
PRIDEiQ9JLK7.

Protocols and materials databases

DNASUi29867.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031519; ENSMUSP00000031519; ENSMUSG00000029544. [Q9JLK7-1]
ENSMUST00000112112; ENSMUSP00000107740; ENSMUSG00000029544. [Q9JLK7-2]
GeneIDi29867.
KEGGimmu:29867.
UCSCiuc008zdg.1. mouse. [Q9JLK7-1]
uc008zdh.1. mouse. [Q9JLK7-2]

Organism-specific databases

CTDi9478.
MGIiMGI:1352750. Cabp1.

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118901.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiQ9JLK7.
OMAiVLAQNCA.
OrthoDBiEOG7XM303.
PhylomeDBiQ9JLK7.
TreeFamiTF334804.

Miscellaneous databases

ChiTaRSiCabp1. mouse.
PROiQ9JLK7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JLK7.
CleanExiMM_CABP1.
ExpressionAtlasiQ9JLK7. baseline and differential.
GenevisibleiQ9JLK7. MM.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Five members of a novel Ca(2+)-binding protein (CABP) subfamily with similarity to calmodulin."
    Haeseleer F., Sokal I., Verlinde C.L.M.J., Erdjument-Bromage H., Tempst P., Pronin A.N., Benovic J.L., Fariss R.N., Palczewski K.
    J. Biol. Chem. 275:1247-1260(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS L-CABP1 AND S-CABP1).
    Tissue: Retina.
  2. "Inhibition of TRPC5 channels by Ca2+-binding protein 1 in Xenopus oocytes."
    Kinoshita-Kawada M., Tang J., Xiao R., Kaneko S., Foskett J.K., Zhu M.X.
    Pflugers Arch. 450:345-354(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TRPC5.
  3. "Switching of Ca2+-dependent inactivation of Ca(v)1.3 channels by calcium binding proteins of auditory hair cells."
    Yang P.S., Alseikhan B.A., Hiel H., Grant L., Mori M.X., Yang W., Fuchs P.A., Yue D.T.
    J. Neurosci. 26:10677-10689(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CACNA1D.
  4. "Ca2+-binding proteins tune Ca2+-feedback to Cav1.3 channels in mouse auditory hair cells."
    Cui G., Meyer A.C., Calin-Jageman I., Neef J., Haeseleer F., Moser T., Lee A.
    J. Physiol. (Lond.) 585:791-803(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CACNA1D.
  5. Cited for: INTERACTION WITH MYO1C.
  6. "A mouse protein that localizes to acrosome and sperm tail is regulated by Y-chromosome."
    Bhattacharya R., Devi M.S., Dhople V.M., Jesudasan R.A.
    BMC Cell Biol. 14:50-50(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH C9ORF9 HOMOLOG.

Entry informationi

Entry nameiCABP1_MOUSE
AccessioniPrimary (citable) accession number: Q9JLK7
Secondary accession number(s): Q9JLK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.