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Protein

Selenoprotein K

Gene

Selk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for Ca2+ flux in immune cells and plays a role in T-cell proliferation and in T-cell and neutrophil migration. Involved in endoplasmic reticulum-associated degradation (ERAD) of soluble glycosylated proteins. Required for palmitoylation and cell surface expression of CD36 and involved in macrophage uptake of low-density lipoprotein and in foam cell formation. Plays a role in protection of cells from ER stress-induced apoptosis. Protects cells from oxidative stress when overexpressed in cardiomyocytes.2 Publications

GO - Molecular functioni

GO - Biological processi

  • calcium ion transport Source: MGI
  • intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: MGI
  • macrophage derived foam cell differentiation Source: MGI
  • positive regulation of cell migration Source: MGI
  • positive regulation of chemokine secretion Source: UniProtKB
  • positive regulation of defense response to virus by host Source: UniProtKB
  • positive regulation of interleukin-6 secretion Source: UniProtKB
  • positive regulation of monocyte chemotactic protein-1 production Source: UniProtKB
  • positive regulation of T cell migration Source: MGI
  • positive regulation of T cell proliferation Source: MGI
  • positive regulation of tumor necrosis factor production Source: UniProtKB
  • protein palmitoylation Source: MGI
  • regulation of calcium-mediated signaling Source: UniProtKB
  • regulation of protein transport Source: MGI
  • respiratory burst after phagocytosis Source: MGI
  • response to oxidative stress Source: MGI
Complete GO annotation...

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Selenoprotein K
Short name:
SelK
Gene namesi
Name:Selk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1931466. Selk.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei20 – 4223HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Normal growth, fertility and immune system development. Reduced receptor-mediated Ca2+ flux in T-cells, neutrophils and macrophages. Reduced T-cell proliferation and reduced T-cell and neutrophil migration. Decreased viral clearance and increased mortality following infection with West Nile virus. Bone marrow-derived macrophages from knockout mice display decreased uptake of acetylated or oxidized low-density lipoprotein, reduced foam cell formation and decreased palmitoylation and cell surface expression of CD36.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 9493Selenoprotein KPRO_0000097670Add
BLAST

Post-translational modificationi

Cleaved by CAPN2/m-calpain in resting macrophages but not in activated macrophages. Macrophage activation up-regulates expression of the calpain inhibitor CAST/calpastatin, resulting in inhibition of CAPN2 activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei81 – 822Cleavage; by CAPN2

Proteomic databases

EPDiQ9JLJ1.
MaxQBiQ9JLJ1.
PaxDbiQ9JLJ1.
PRIDEiQ9JLJ1.

PTM databases

PhosphoSiteiQ9JLJ1.

Expressioni

Tissue specificityi

High expression in spleen and intestine (at protein level). Expressed in a range of immune cells including T and B-cells and also in myeloid cells including macrophages, neutrophils and dendritic cells (at protein level).2 Publications

Inductioni

By increased dietary selenium. Expression is significantly decreased by a low selenium diet.1 Publication

Gene expression databases

BgeeiQ9JLJ1.
CleanExiMM_SELK.
GenevisibleiQ9JLJ1. MM.

Interactioni

Subunit structurei

Interacts with DERL1, DERL2, DERL3 and VIMP/SELS. The SELK-VIMP complex interacts with VCP. Part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000107887.

Family & Domainsi

Sequence similaritiesi

Belongs to the selenoprotein K family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J0AZ. Eukaryota.
ENOG410XWKI. LUCA.
GeneTreeiENSGT00390000016119.
HOGENOMiHOG000013002.
HOVERGENiHBG108467.
InParanoidiQ9JLJ1.
OMAiMGRINHW.
OrthoDBiEOG747PN7.
PhylomeDBiQ9JLJ1.
TreeFamiTF328380.

Family and domain databases

InterProiIPR024491. Se_SelK/SelG.
[Graphical view]
PfamiPF10961. DUF2763. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JLJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVYISNGQVL DSRNQSPWRV SFLTDFFWGI AEFVVFFFKT LLQQDVKKRR
60 70 80 90
GYGSSSDSRY DDGRGPPGNP PRRMGRISHL RGPSPPPMAG GUGR
Length:94
Mass (Da):10,642
Last modified:February 26, 2008 - v3
Checksum:i840B51DC9CF7B8F2
GO

Sequence cautioni

The sequence AAF27311.1 differs from that shown. Reason: Erroneous termination at position 92. Translated as Sec.Curated

Non-standard residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-standard residuei92 – 921SelenocysteineBy similarity

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF170920 mRNA. Translation: AAF27311.1. Sequence problems.
AK003206 mRNA. Translation: BAB22642.2.
AK006674 mRNA. Translation: BAB24699.2.
AK007523 mRNA. Translation: BAB25088.2.
AK008419 mRNA. Translation: BAB25657.2.
BC038049 mRNA. Translation: AAH38049.3.
BC096044 mRNA. Translation: AAH96044.1.
BC096677 mRNA. Translation: AAH96677.2.
CCDSiCCDS36844.1.
RefSeqiNP_064363.2. NM_019979.2.
UniGeneiMm.104491.

Genome annotation databases

EnsembliENSMUST00000112268; ENSMUSP00000107887; ENSMUSG00000042682.
GeneIDi80795.
KEGGimmu:80795.
UCSCiuc007sul.1. mouse.

Keywords - Coding sequence diversityi

Selenocysteine

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF170920 mRNA. Translation: AAF27311.1. Sequence problems.
AK003206 mRNA. Translation: BAB22642.2.
AK006674 mRNA. Translation: BAB24699.2.
AK007523 mRNA. Translation: BAB25088.2.
AK008419 mRNA. Translation: BAB25657.2.
BC038049 mRNA. Translation: AAH38049.3.
BC096044 mRNA. Translation: AAH96044.1.
BC096677 mRNA. Translation: AAH96677.2.
CCDSiCCDS36844.1.
RefSeqiNP_064363.2. NM_019979.2.
UniGeneiMm.104491.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000107887.

PTM databases

PhosphoSiteiQ9JLJ1.

Proteomic databases

EPDiQ9JLJ1.
MaxQBiQ9JLJ1.
PaxDbiQ9JLJ1.
PRIDEiQ9JLJ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000112268; ENSMUSP00000107887; ENSMUSG00000042682.
GeneIDi80795.
KEGGimmu:80795.
UCSCiuc007sul.1. mouse.

Organism-specific databases

CTDi58515.
MGIiMGI:1931466. Selk.

Phylogenomic databases

eggNOGiENOG410J0AZ. Eukaryota.
ENOG410XWKI. LUCA.
GeneTreeiENSGT00390000016119.
HOGENOMiHOG000013002.
HOVERGENiHBG108467.
InParanoidiQ9JLJ1.
OMAiMGRINHW.
OrthoDBiEOG747PN7.
PhylomeDBiQ9JLJ1.
TreeFamiTF328380.

Miscellaneous databases

ChiTaRSiSelk. mouse.
NextBioi350133.
PROiQ9JLJ1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JLJ1.
CleanExiMM_SELK.
GenevisibleiQ9JLJ1. MM.

Family and domain databases

InterProiIPR024491. Se_SelK/SelG.
[Graphical view]
PfamiPF10961. DUF2763. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Xie L.P., Chen W.F.
    Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Thymic epithelium.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo, Pancreas, Small intestine and Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and Czech II.
    Tissue: Mammary gland and Mammary tumor.
  4. "Selenoprotein K is a novel target of m-calpain, and cleavage is regulated by Toll-like receptor-induced calpastatin in macrophages."
    Huang Z., Hoffmann F.W., Norton R.L., Hashimoto A.C., Hoffmann P.R.
    J. Biol. Chem. 286:34830-34838(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, CLEAVAGE BY CAPN2.
  5. "Selenoprotein K binds multiprotein complexes and is involved in the regulation of endoplasmic reticulum homeostasis."
    Shchedrina V.A., Everley R.A., Zhang Y., Gygi S.P., Hatfield D.L., Gladyshev V.N.
    J. Biol. Chem. 286:42937-42948(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DERL1; DERL2 AND DERL3.
  6. "Selenoprotein K knockout mice exhibit deficient calcium flux in immune cells and impaired immune responses."
    Verma S., Hoffmann F.W., Kumar M., Huang Z., Roe K., Nguyen-Wu E., Hashimoto A.S., Hoffmann P.R.
    J. Immunol. 186:2127-2137(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.
  7. "Selenoprotein K is required for palmitoylation of CD36 in macrophages: implications in foam cell formation and atherogenesis."
    Meiler S., Baumer Y., Huang Z., Hoffmann F.W., Fredericks G.J., Rose A.H., Norton R.L., Hoffmann P.R., Boisvert W.A.
    J. Leukoc. Biol. 93:771-780(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiSELK_MOUSE
AccessioniPrimary (citable) accession number: Q9JLJ1
Secondary accession number(s): Q8CI00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 4, 2003
Last sequence update: February 26, 2008
Last modified: March 16, 2016
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.