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Q9JLJ0 (LITAF_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog

Short name=LPS-induced TNF-alpha factor homolog
Alternative name(s):
Estrogen-enhanced transcript protein
Short name=mEET
LITAF-like protein
NEDD4 WW domain-binding protein 3
Gene names
Name:Litaf
Synonyms:N4wbp3, Tbx1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length161 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable role in regulating transcription of specific genes. May regulate through NFKB1 the expression of the CCL2/MCP-1 chemokine. May play a role in tumor necrosis factor alpha (TNF-alpha) gene expression. Ref.1 Ref.2

Subunit structure

Interacts with WWOX By similarity. Interacts with NEDD4. Ref.5

Subcellular location

Lysosome membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Associated with membranes of lysosomes By similarity. Ref.1

Tissue specificity

Widely expressed. Highly expressed in liver. Ref.1 Ref.2

Developmental stage

Strong expression is detected at E.7 and drops at E11. Ref.2

Induction

By estrogen and lipopolysaccharides (LPS). Ref.1 Ref.2

Domain

The WW-binding motif mediates interaction with NEDD4, and probably WWOX By similarity. Ref.5

Sequence similarities

Belongs to the CDIP1/LITAF family.

Sequence caution

The sequence AAG44246.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processApoptosis
Transcription
Transcription regulation
   Cellular componentLysosome
Membrane
   DiseaseProto-oncogene
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaging

Inferred from electronic annotation. Source: Ensembl

apoptotic process

Inferred from electronic annotation. Source: UniProtKB-KW

cellular response to lipopolysaccharide

Inferred from mutant phenotype PubMed 16954198. Source: MGI

negative regulation of NF-kappaB import into nucleus

Inferred from mutant phenotype PubMed 12914755. Source: MGI

positive regulation of I-kappaB kinase/NF-kappaB signaling

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of cytokine production

Inferred from mutant phenotype PubMed 16954198. Source: MGI

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

response to lipopolysaccharide

Inferred from mutant phenotype PubMed 22160695. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentGolgi apparatus

Inferred from sequence or structural similarity. Source: UniProtKB

lysosomal membrane

Inferred from sequence orthology PubMed 11274176. Source: MGI

plasma membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionWW domain binding

Inferred from sequence or structural similarity. Source: UniProtKB

signal transducer activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Nedd4P469355EBI-643664,EBI-773516

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 161161Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog
PRO_0000084441

Regions

Motif20 – 234WW-binding

Experimental info

Mutagenesis231Y → A: Abolishes interaction with NEDD4. Ref.5
Mutagenesis611Y → A: No effect on interaction with NEDD4. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q9JLJ0 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 7397F79C5AD0CD79

FASTA16116,946
        10         20         30         40         50         60 
MSAPGPYQAA AGPSVVPTAP PTYEETVGVN SYYPTPPAPM PGPATGLITG PDGKGMNPPS 

        70         80         90        100        110        120 
YYTQPVPVPN ANAIAVQTVY VQQPVSFYDR PVQMCCPSCS KMIVTQLSYN AGALTWLSCG 

       130        140        150        160 
SLCLLGCVAG CCFIPFCVDA LQDVDHYCPN CKALLGTYKR L 

« Hide

References

« Hide 'large scale' references
[1]"Negative regulation of monocyte chemoattractant protein-1 gene expression by a mouse estrogen-enhanced transcript."
Xie L.-P., Fu W.-X., Jin C., Dong X.-Y., Chen W.-F.
Eur. J. Immunol. 32:2837-2846(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, SUBCELLULAR LOCATION.
Tissue: Thymus.
[2]"Molecular cloning and characterization of mouse LITAF cDNA: role in the regulation of tumor necrosis factor-alpha (TNF-alpha) gene expression."
Bolcato-Bellemin A.-L., Mattei M.-G., Fenton M., Amar S.
J. Endotoxin Res. 10:15-23(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, DEVELOPMENTAL STAGE.
Tissue: Heart.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Bone marrow, Colon and Head.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[5]"Identification of multiple proteins expressed in murine embryos as binding partners for the WW domains of the ubiquitin-protein ligase Nedd4."
Jolliffe C.N., Harvey K.F., Haines B.P., Parasivam G., Kumar S.
Biochem. J. 351:557-565(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-130, INTERACTION WITH NEDD4, MUTAGENESIS OF TYR-23 AND TYR-61, DOMAIN.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF171100 mRNA. Translation: AAF27312.1.
AF230522 mRNA. Translation: AAO49168.1.
AK018578 mRNA. Translation: BAB31289.1.
AK076162 mRNA. Translation: BAC36228.1.
AK151053 mRNA. Translation: BAE30070.1.
AK159533 mRNA. Translation: BAE35161.1.
BC018559 mRNA. Translation: AAH18559.1.
AF220207 mRNA. Translation: AAG44246.1. Different initiation.
RefSeqNP_064364.1. NM_019980.2.
XP_006522490.1. XM_006522427.1.
UniGeneMm.294753.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid208144. 2 interactions.
IntActQ9JLJ0. 2 interactions.
MINTMINT-147796.

PTM databases

PhosphoSiteQ9JLJ0.

Proteomic databases

PRIDEQ9JLJ0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023143; ENSMUSP00000023143; ENSMUSG00000022500.
ENSMUST00000117360; ENSMUSP00000112667; ENSMUSG00000022500.
GeneID56722.
KEGGmmu:56722.
UCSCuc007yen.2. mouse.

Organism-specific databases

CTD9516.
MGIMGI:1929512. Litaf.

Phylogenomic databases

eggNOGNOG41852.
GeneTreeENSGT00540000071542.
HOGENOMHOG000039585.
HOVERGENHBG006272.
InParanoidQ9JLJ0.
OMACIAGCCL.
OrthoDBEOG7XH6RR.
PhylomeDBQ9JLJ0.
TreeFamTF313294.

Gene expression databases

ArrayExpressQ9JLJ0.
BgeeQ9JLJ0.
CleanExMM_LITAF.
MM_TBX1.
GenevestigatorQ9JLJ0.

Family and domain databases

InterProIPR006629. LITAF.
[Graphical view]
PfamPF10601. zf-LITAF-like. 1 hit.
[Graphical view]
SMARTSM00714. LITAF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio313198.
PROQ9JLJ0.
SOURCESearch...

Entry information

Entry nameLITAF_MOUSE
AccessionPrimary (citable) accession number: Q9JLJ0
Secondary accession number(s): Q9EQI0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot