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Protein

Squamous cell carcinoma antigen recognized by T-cells 3

Gene

Sart3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

U6 snRNP-binding protein that functions as a recycling factor of the splicing machinery. Promotes the initial reassembly of U4 and U6 snRNPs following their ejection from the spliceosome during its maturation. Also binds U6atac snRNPs and may function as a recycling factor for U4atac/U6atac spliceosomal snRNP, an initial step in the assembly of U12-type spliceosomal complex. The U12-type spliceosomal complex plays a role in the splicing of introns with non-canonical splice sites. May also function as a substrate-targeting factor for deubiquitinases like USP4 and USP15. Recruits USP4 to ubiquitinated PRPF3 within the U4/U5/U6 tri-snRNP complex, promoting PRPF3 deubiquitination and thereby regulating the spliceosome U4/U5/U6 tri-snRNP spliceosomal complex disassembly. May also recruit the deubiquitinase USP15 to histone H2B and mediate histone deubiquitination, thereby regulating gene expression and/or DNA repair (By similarity). May play a role in hematopoiesis probably through transcription regulation of specific genes including MYC (PubMed:21447833).By similarity1 Publication

GO - Molecular functioni

  1. histone binding Source: UniProtKB
  2. nucleotide binding Source: InterPro
  3. poly(A) RNA binding Source: MGI
  4. RNA binding Source: MGI
  5. U6atac snRNA binding Source: UniProtKB
  6. U6 snRNA binding Source: UniProtKB
  7. ubiquitin-specific protease binding Source: MGI

GO - Biological processi

  1. cell morphogenesis Source: MGI
  2. hematopoietic stem cell proliferation Source: MGI
  3. homeostasis of number of cells Source: MGI
  4. mRNA splicing, via spliceosome Source: UniProtKB
  5. nucleosome assembly Source: UniProtKB
  6. positive regulation of histone deubiquitination Source: UniProtKB
  7. regulation of gene expression Source: MGI
  8. spliceosomal snRNP assembly Source: UniProtKB
  9. spliceosomal tri-snRNP complex assembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Squamous cell carcinoma antigen recognized by T-cells 3Curated
Short name:
SART-31 Publication
Short name:
mSART-31 Publication
Alternative name(s):
Tumor-rejection antigen SART31 Publication
Gene namesi
Name:Sart3Imported
Synonyms:Kiaa0156Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1858230. Sart3.

Subcellular locationi

Nucleusnucleoplasm 1 Publication. NucleusCajal body 1 Publication. Nucleus speckle By similarity. Cytoplasm By similarity

GO - Cellular componenti

  1. Cajal body Source: UniProtKB
  2. cytoplasm Source: UniProtKB-SubCell
  3. nuclear speck Source: UniProtKB-SubCell
  4. nucleoplasm Source: UniProtKB
  5. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Knockout of Sart3 is embryonic lethal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 962961Squamous cell carcinoma antigen recognized by T-cells 3PRO_0000223314Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei10 – 101PhosphoserineBy similarity
Modified residuei852 – 8521PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9JLI8.
PaxDbiQ9JLI8.
PRIDEiQ9JLI8.

PTM databases

PhosphoSiteiQ9JLI8.

Expressioni

Tissue specificityi

Ubiquitously expressed, with low level of expression in liver, heart and skeletal (PubMed:10761712). Also detected in hematopoietic cells (at protein level) (PubMed:21447833).2 Publications

Developmental stagei

Expressed from early prenatal stages, as early as E7 and increased thereafter.1 Publication

Inductioni

Up-regulated in proliferating hematopoietic cells.1 Publication

Gene expression databases

BgeeiQ9JLI8.
GenevestigatoriQ9JLI8.

Interactioni

Subunit structurei

Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with AGO1 and AGO2. Interacts with PRPF3 and USP4; the interaction with PRPF3 is direct and recruits USP4 to its substrate PRPF3. Interacts with USP15; the interaction is direct.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9JLI8.
SMRiQ9JLI8. Positions 103-361, 700-902.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati127 – 15933HAT 1Sequence AnalysisAdd
BLAST
Repeati165 – 19632HAT 2Sequence AnalysisAdd
BLAST
Repeati202 – 23837HAT 3Sequence AnalysisAdd
BLAST
Repeati243 – 27634HAT 4Sequence AnalysisAdd
BLAST
Repeati325 – 35733HAT 5Sequence AnalysisAdd
BLAST
Repeati360 – 39233HAT 6Sequence AnalysisAdd
BLAST
Repeati395 – 43137HAT 7Sequence AnalysisAdd
BLAST
Repeati441 – 47434HAT 8Sequence AnalysisAdd
BLAST
Repeati488 – 52134HAT 9Sequence AnalysisAdd
BLAST
Domaini704 – 78279RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini801 – 87878RRM 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 352351Mediates interaction with PRPF3By similarityAdd
BLAST
Regioni488 – 52134Required for interaction with USP4By similarityAdd
BLAST
Regioni538 – 952415Necessary and sufficient for U6 snRNA bindingBy similarityAdd
BLAST
Regioni601 – 67070Required for nuclear localizationBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili559 – 61860Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 9 HAT repeats.Curated
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiCOG0724.
GeneTreeiENSGT00730000111098.
HOGENOMiHOG000063708.
HOVERGENiHBG053888.
InParanoidiQ9JLI8.
OMAiYYNLESP.
OrthoDBiEOG72ZCDB.
PhylomeDBiQ9JLI8.
TreeFamiTF317554.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR003107. HAT.
IPR008669. LSM_interact.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF05391. Lsm_interact. 1 hit.
PF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00386. HAT. 7 hits.
SM00360. RRM. 2 hits.
[Graphical view]
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JLI8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATTAASSAS EPEVEPQAGP EAEGEEDEAK PAGVQRKVLS GAVAAEAAEA
60 70 80 90 100
KGPGWDLQRE GASGSDGDEE DAMASSAESS AGEDEWEYDE EEEKNQLEIE
110 120 130 140 150
RLEEQLSING YDYNCHVELI RLLRLEGELS RVRAARQKMS ELFPLTEELW
160 170 180 190 200
LEWLHDEISM AMDGLDREHV YELFERAVKD YICPNIWLEY GQYSVGGIGQ
210 220 230 240 250
KGGLEKVRSV FERALSSVGL HMTKGLAIWE AYREFESAIV EAARLEKVHS
260 270 280 290 300
LFRRQLAIPL YEMEATFAEY EEWSEEPMPE SVLQSYQKAL GQLEKYKPYE
310 320 330 340 350
EALLQAEAPR LAEYQAYIDF EMKIGDPARI QLIFERALVE NCLVPDLWIR
360 370 380 390 400
YSQYLDRQLK VKDLVLSVHS RAVRNCPWTV ALWSRYLLAM ERHGLDHQTI
410 420 430 440 450
SATFENALSA GFIQATDYVE IWQVYLDYLR RRVDFRQDSS KELEELRSMF
460 470 480 490 500
TRALEYLQQE VEERFSESGD PSCLIMQSWA RVEARLCNNM QKARELWDSI
510 520 530 540 550
MTRGNAKYAN MWLEYYNLER AHGDTQHCRK ALHRAVQCTS DYPEHVCEVL
560 570 580 590 600
LTMERTEGTL EDWDLAIQKT ETRLARVNEQ RMKAAEKEAA LVQQEEEKAE
610 620 630 640 650
QRKKVRAEKK ALKKKKKTRG ADKRREDEDE ENEWGEEEEE QPSKRRRTEN
660 670 680 690 700
SLASGEASAM KEETELSGKC LTIDVGPPSK QKEKAASLKR DMPKVAHDSS
710 720 730 740 750
KDSVTVFVSN LPYSIEEPEV KLRPLFEVCG EVVQIRPIFS NRGDFRGYCY
760 770 780 790 800
VEFGEEKSAQ QALELDRKIV EGRPMFVSPC VDKSKNPDFK VFRYSTTLEK
810 820 830 840 850
HKLFISGLPF SCTKEELEDI CKAHGTVKDL RLVTNRAGKP KGLAYVEYEN
860 870 880 890 900
ESQASQAVMK MDGMTIRENV IKVAISNPPQ RKVPEKPEVR TAPGAPMLPR
910 920 930 940 950
QMYGARGKGR TQLSLLPRAL QRQGAAPQAE NGPAPGPAVA PSVATEAPKM
960
SNADFAKLLL RK
Length:962
Mass (Da):109,619
Last modified:October 1, 2000 - v1
Checksum:i23BC235125E7A09C
GO
Isoform 2 (identifier: Q9JLI8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     356-401: DRQLKVKDLV...RHGLDHQTIS → PCCAELPMDS...RSECRLHPGH
     402-962: Missing.

Note: No experimental confirmation available.

Show »
Length:401
Mass (Da):45,291
Checksum:iAE0B8FA9BE117FFE
GO

Sequence cautioni

The sequence AAH36350.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti160 – 1601M → V in BAC39661 (PubMed:16141072).Curated
Sequence conflicti792 – 81423FRYST…FSCTK → CFLKKGVFRVGCPIGSAQ in BAC97877 (PubMed:14621295).CuratedAdd
BLAST

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei356 – 40146DRQLK…HQTIS → PCCAELPMDSCPVESVPSGH GATWTGPSNDFCDLRERSEC RLHPGH in isoform 2. 1 PublicationVSP_017252Add
BLAST
Alternative sequencei402 – 962561Missing in isoform 2. 1 PublicationVSP_017253Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF172722 mRNA. Translation: AAF65228.1.
AK053828 mRNA. Translation: BAC35544.1.
AK129067 Transcribed RNA. Translation: BAC97877.2.
AK086398 mRNA. Translation: BAC39661.1.
BC036350 mRNA. Translation: AAH36350.1. Different initiation.
BC057156 mRNA. Translation: AAH57156.1.
CCDSiCCDS19552.1. [Q9JLI8-1]
RefSeqiNP_058622.1. NM_016926.1. [Q9JLI8-1]
UniGeneiMm.29594.

Genome annotation databases

EnsembliENSMUST00000019118; ENSMUSP00000019118; ENSMUSG00000018974. [Q9JLI8-1]
GeneIDi53890.
KEGGimmu:53890.
UCSCiuc008yym.1. mouse. [Q9JLI8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF172722 mRNA. Translation: AAF65228.1.
AK053828 mRNA. Translation: BAC35544.1.
AK129067 Transcribed RNA. Translation: BAC97877.2.
AK086398 mRNA. Translation: BAC39661.1.
BC036350 mRNA. Translation: AAH36350.1. Different initiation.
BC057156 mRNA. Translation: AAH57156.1.
CCDSiCCDS19552.1. [Q9JLI8-1]
RefSeqiNP_058622.1. NM_016926.1. [Q9JLI8-1]
UniGeneiMm.29594.

3D structure databases

ProteinModelPortaliQ9JLI8.
SMRiQ9JLI8. Positions 103-361, 700-902.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ9JLI8.

Proteomic databases

MaxQBiQ9JLI8.
PaxDbiQ9JLI8.
PRIDEiQ9JLI8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019118; ENSMUSP00000019118; ENSMUSG00000018974. [Q9JLI8-1]
GeneIDi53890.
KEGGimmu:53890.
UCSCiuc008yym.1. mouse. [Q9JLI8-1]

Organism-specific databases

CTDi9733.
MGIiMGI:1858230. Sart3.
RougeiSearch...

Phylogenomic databases

eggNOGiCOG0724.
GeneTreeiENSGT00730000111098.
HOGENOMiHOG000063708.
HOVERGENiHBG053888.
InParanoidiQ9JLI8.
OMAiYYNLESP.
OrthoDBiEOG72ZCDB.
PhylomeDBiQ9JLI8.
TreeFamiTF317554.

Miscellaneous databases

ChiTaRSiSart3. mouse.
NextBioi310779.
PROiQ9JLI8.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JLI8.
GenevestigatoriQ9JLI8.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR003107. HAT.
IPR008669. LSM_interact.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF05391. Lsm_interact. 1 hit.
PF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00386. HAT. 7 hits.
SM00360. RRM. 2 hits.
[Graphical view]
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse homologue of the human SART3 gene encoding tumor-rejection antigen."
    Harada K., Yamada A., Mine T., Kawagoe N., Takasu H., Itoh K.
    Jpn. J. Cancer Res. 91:239-247(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: BALB/c.
    Tissue: Squamous cell carcinoma.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Embryonic tail.
  3. Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.
    Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Eye and Head.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: NMRI.
    Tissue: Mammary gland and Mammary tumor.
  6. "Identification of a gene coding for a protein possessing shared tumor epitopes capable of inducing HLA-A24-restricted cytotoxic T lymphocytes in cancer patients."
    Yang D., Nakao M., Shichijo S., Sasatomi T., Takasu H., Matsumoto H., Mori K., Hayashi A., Yamana H., Shirouzu K., Itoh K.
    Cancer Res. 59:4056-4063(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: REGION.
  7. "Targeting of U4/U6 small nuclear RNP assembly factor SART3/p110 to Cajal bodies."
    Stanek D., Rader S.D., Klingauf M., Neugebauer K.M.
    J. Cell Biol. 160:505-516(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "TIP110/p110nrb/SART3/p110 regulation of hematopoiesis through CMYC."
    Liu Y., Timani K., Mantel C., Fan Y., Hangoc G., Cooper S., He J.J., Broxmeyer H.E.
    Blood 117:5643-5651(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiSART3_MOUSE
AccessioniPrimary (citable) accession number: Q9JLI8
Secondary accession number(s): Q6ZQI2
, Q8BPK9, Q8C3B7, Q8CFU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 1, 2000
Last modified: March 4, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.