Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9JLI8 (SART3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Squamous cell carcinoma antigen recognized by T-cells 3

Short name=SART-3
Short name=mSART-3
Alternative name(s):
Tumor-rejection antigen SART3
Gene names
Name:Sart3
Synonyms:Kiaa0156
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length962 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Subunit structure

Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with AGO1 and AGO2 By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Nucleus speckle By similarity.

Tissue specificity

Ubiquitously expressed, with low level of expression in liver, heart and skeletal. Ref.1

Developmental stage

Expressed from early prenatal stages, as early as E7 and increased thereafter. Ref.1

Sequence similarities

Contains 9 HAT repeats.

Contains 2 RRM (RNA recognition motif) domains.

Sequence caution

The sequence AAH36350.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9JLI8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9JLI8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     356-401: DRQLKVKDLV...RHGLDHQTIS → PCCAELPMDS...RSECRLHPGH
     402-962: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 962961Squamous cell carcinoma antigen recognized by T-cells 3
PRO_0000223314

Regions

Coiled coil559 – 61860 Potential
Motif602 – 61817Nuclear localization signal Potential

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue101Phosphoserine By similarity
Modified residue8521Phosphoserine By similarity

Natural variations

Alternative sequence356 – 40146DRQLK…HQTIS → PCCAELPMDSCPVESVPSGH GATWTGPSNDFCDLRERSEC RLHPGH in isoform 2.
VSP_017252
Alternative sequence402 – 962561Missing in isoform 2.
VSP_017253

Experimental info

Sequence conflict1601M → V in BAC39661. Ref.4
Sequence conflict792 – 81423FRYST…FSCTK → CFLKKGVFRVGCPIGSAQ in BAC97877. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 23BC235125E7A09C

FASTA962109,619
        10         20         30         40         50         60 
MATTAASSAS EPEVEPQAGP EAEGEEDEAK PAGVQRKVLS GAVAAEAAEA KGPGWDLQRE 

        70         80         90        100        110        120 
GASGSDGDEE DAMASSAESS AGEDEWEYDE EEEKNQLEIE RLEEQLSING YDYNCHVELI 

       130        140        150        160        170        180 
RLLRLEGELS RVRAARQKMS ELFPLTEELW LEWLHDEISM AMDGLDREHV YELFERAVKD 

       190        200        210        220        230        240 
YICPNIWLEY GQYSVGGIGQ KGGLEKVRSV FERALSSVGL HMTKGLAIWE AYREFESAIV 

       250        260        270        280        290        300 
EAARLEKVHS LFRRQLAIPL YEMEATFAEY EEWSEEPMPE SVLQSYQKAL GQLEKYKPYE 

       310        320        330        340        350        360 
EALLQAEAPR LAEYQAYIDF EMKIGDPARI QLIFERALVE NCLVPDLWIR YSQYLDRQLK 

       370        380        390        400        410        420 
VKDLVLSVHS RAVRNCPWTV ALWSRYLLAM ERHGLDHQTI SATFENALSA GFIQATDYVE 

       430        440        450        460        470        480 
IWQVYLDYLR RRVDFRQDSS KELEELRSMF TRALEYLQQE VEERFSESGD PSCLIMQSWA 

       490        500        510        520        530        540 
RVEARLCNNM QKARELWDSI MTRGNAKYAN MWLEYYNLER AHGDTQHCRK ALHRAVQCTS 

       550        560        570        580        590        600 
DYPEHVCEVL LTMERTEGTL EDWDLAIQKT ETRLARVNEQ RMKAAEKEAA LVQQEEEKAE 

       610        620        630        640        650        660 
QRKKVRAEKK ALKKKKKTRG ADKRREDEDE ENEWGEEEEE QPSKRRRTEN SLASGEASAM 

       670        680        690        700        710        720 
KEETELSGKC LTIDVGPPSK QKEKAASLKR DMPKVAHDSS KDSVTVFVSN LPYSIEEPEV 

       730        740        750        760        770        780 
KLRPLFEVCG EVVQIRPIFS NRGDFRGYCY VEFGEEKSAQ QALELDRKIV EGRPMFVSPC 

       790        800        810        820        830        840 
VDKSKNPDFK VFRYSTTLEK HKLFISGLPF SCTKEELEDI CKAHGTVKDL RLVTNRAGKP 

       850        860        870        880        890        900 
KGLAYVEYEN ESQASQAVMK MDGMTIRENV IKVAISNPPQ RKVPEKPEVR TAPGAPMLPR 

       910        920        930        940        950        960 
QMYGARGKGR TQLSLLPRAL QRQGAAPQAE NGPAPGPAVA PSVATEAPKM SNADFAKLLL 


RK 

« Hide

Isoform 2 [UniParc].

Checksum: AE0B8FA9BE117FFE
Show »

FASTA40145,291

References

« Hide 'large scale' references
[1]"Mouse homologue of the human SART3 gene encoding tumor-rejection antigen."
Harada K., Yamada A., Mine T., Kawagoe N., Takasu H., Itoh K.
Jpn. J. Cancer Res. 91:239-247(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: BALB/c.
Tissue: Squamous cell carcinoma.
[2]"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Embryonic tail.
[3]Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.
Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Eye and Head.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: NMRI.
Tissue: Mammary gland and Mammary tumor.
[6]"Identification of a gene coding for a protein possessing shared tumor epitopes capable of inducing HLA-A24-restricted cytotoxic T lymphocytes in cancer patients."
Yang D., Nakao M., Shichijo S., Sasatomi T., Takasu H., Matsumoto H., Mori K., Hayashi A., Yamana H., Shirouzu K., Itoh K.
Cancer Res. 59:4056-4063(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: REGION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF172722 mRNA. Translation: AAF65228.1.
AK053828 mRNA. Translation: BAC35544.1.
AK129067 Transcribed RNA. Translation: BAC97877.2.
AK086398 mRNA. Translation: BAC39661.1.
BC036350 mRNA. Translation: AAH36350.1. Different initiation.
BC057156 mRNA. Translation: AAH57156.1.
CCDSCCDS19552.1. [Q9JLI8-1]
RefSeqNP_058622.1. NM_016926.1. [Q9JLI8-1]
UniGeneMm.29594.

3D structure databases

ProteinModelPortalQ9JLI8.
SMRQ9JLI8. Positions 103-361, 702-877.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ9JLI8.

Proteomic databases

MaxQBQ9JLI8.
PaxDbQ9JLI8.
PRIDEQ9JLI8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000019118; ENSMUSP00000019118; ENSMUSG00000018974. [Q9JLI8-1]
GeneID53890.
KEGGmmu:53890.
UCSCuc008yym.1. mouse. [Q9JLI8-1]

Organism-specific databases

CTD9733.
MGIMGI:1858230. Sart3.
RougeSearch...

Phylogenomic databases

eggNOGCOG0724.
GeneTreeENSGT00730000111098.
HOGENOMHOG000063708.
HOVERGENHBG053888.
InParanoidQ9JLI8.
OMAYYNLESP.
OrthoDBEOG72ZCDB.
PhylomeDBQ9JLI8.
TreeFamTF317554.

Gene expression databases

BgeeQ9JLI8.
GenevestigatorQ9JLI8.

Family and domain databases

Gene3D3.30.70.330. 2 hits.
InterProIPR003107. HAT.
IPR008669. LSM_interact.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamPF05391. Lsm_interact. 1 hit.
PF00076. RRM_1. 2 hits.
[Graphical view]
SMARTSM00386. HAT. 7 hits.
SM00360. RRM. 2 hits.
[Graphical view]
PROSITEPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSART3. mouse.
NextBio310779.
PROQ9JLI8.
SOURCESearch...

Entry information

Entry nameSART3_MOUSE
AccessionPrimary (citable) accession number: Q9JLI8
Secondary accession number(s): Q6ZQI2 expand/collapse secondary AC list , Q8BPK9, Q8C3B7, Q8CFU9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot