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Protein

Sperm-associated antigen 6

Gene

Spag6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Important for structural integrity of the central apparatus in the sperm tail and for flagellar motility.2 Publications

GO - Biological processi

  • flagellated sperm motility Source: MGI
  • sperm axoneme assembly Source: MGI
  • ventricular system development Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm-associated antigen 6
Alternative name(s):
Axoneme central apparatus protein
Protein PF16 homolog
Gene namesi
Name:Spag6
Synonyms:Pf16
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1354388. Spag6.

Subcellular locationi

GO - Cellular componenti

  • axonemal central apparatus Source: MGI
  • cytoplasm Source: UniProtKB-KW
  • microtubule Source: UniProtKB-KW
  • microtubule cytoskeleton Source: MGI
  • nucleus Source: MGI
  • sperm principal piece Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum, Microtubule

Pathology & Biotechi

Involvement in diseasei

Defects in Spag6 are a cause of hydrocephalus and of male infertility.

Disruption phenotypei

In null embryos the central apparatus is unstable leading to a loss, and presumably degradation of proteins to which SPAG6 normally binds, including SPAG16 AND SPAG17.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000720961 – 507Sperm-associated antigen 6Add BLAST507

Proteomic databases

MaxQBiQ9JLI7.
PaxDbiQ9JLI7.
PRIDEiQ9JLI7.

PTM databases

PhosphoSitePlusiQ9JLI7.

Expressioni

Tissue specificityi

Highly expressed in testis. Not detected in prostate, ovary, spleen, thymus, small intestine, colon and peripheral blood leukocytes.2 Publications

Gene expression databases

BgeeiENSMUSG00000022783.
CleanExiMM_SPAG6.
ExpressionAtlasiQ9JLI7. baseline and differential.
GenevisibleiQ9JLI7. MM.

Interactioni

Subunit structurei

Interacts with SPAG16 AND SPAG17.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Spag17Q5S0033EBI-1783654,EBI-1783665

Protein-protein interaction databases

BioGridi206052. 1 interactor.
IntActiQ9JLI7. 1 interactor.
STRINGi10090.ENSMUSP00000023468.

Structurei

3D structure databases

ProteinModelPortaliQ9JLI7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati31 – 70ARM 1Add BLAST40
Repeati73 – 112ARM 2Add BLAST40
Repeati115 – 154ARM 3Add BLAST40
Repeati157 – 196ARM 4Add BLAST40
Repeati199 – 238ARM 5Add BLAST40
Repeati241 – 280ARM 6Add BLAST40
Repeati325 – 365ARM 7Add BLAST41
Repeati402 – 441ARM 8Add BLAST40

Sequence similaritiesi

Contains 8 ARM repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0166. Eukaryota.
COG5064. LUCA.
GeneTreeiENSGT00760000119094.
HOGENOMiHOG000257051.
HOVERGENiHBG055326.
InParanoidiQ9JLI7.
OMAiRVENYQP.
OrthoDBiEOG091G07CA.
PhylomeDBiQ9JLI7.
TreeFamiTF328894.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR000357. HEAT.
[Graphical view]
PfamiPF00514. Arm. 4 hits.
PF02985. HEAT. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9JLI7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQRQVLQVF EQYQKARTQF VQMVAELATR PQNIETLQNA GVMSLLRPLL
60 70 80 90 100
LDVVPTIQQT AALALGRLAN YNDDLAEAVV KGDILPQLVY SLAEQNRFYK
110 120 130 140 150
KAAAFVLRAV GKHSPQLAQA IVDCGALDTL VICLEDFDPG VKEAAAWALG
160 170 180 190 200
YIARHNTELS QAVVDAGAIP LLVLCIQEPE IALKRIAASA LSDISKHSPE
210 220 230 240 250
LAQTVVDAGA IAHLAQMILN PDAKLKRQVL SALSQIAKHS VDLAEMVVEA
260 270 280 290 300
EIFPVVLTCL KDKDEYVKKN ACTLIREIAK HTPELSQLIV NAGGVAAVID
310 320 330 340 350
CIGSCKGNIR LPGIMMLGYV AAHSENLAMA VIISKGVPQL SICLSEEPED
360 370 380 390 400
HIKAAAAWAL GQLGRHTPEH ARAVAVTNTL PVLLSLYMSP ESSEDLQLKS
410 420 430 440 450
KKAIKNILQK CTYLPALEPF LYDAPPNILK HVVGQFSKVL PHDSKARRLF
460 470 480 490 500
VTSGGLKKVQ EIKAEPGSLL QEYINSINNC YPEEIVRYYS PGYSDTLLQR

VDSYQPL
Length:507
Mass (Da):55,269
Last modified:October 1, 2000 - v1
Checksum:iEFFAE719EC111285
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF173866 mRNA. Translation: AAF35831.1.
AK005732 mRNA. Translation: BAB24211.1.
AF486266 Genomic DNA. Translation: AAM64149.1.
CCDSiCCDS27988.1.
RefSeqiNP_056588.1. NM_015773.2.
UniGeneiMm.31701.

Genome annotation databases

EnsembliENSMUST00000023468; ENSMUSP00000023468; ENSMUSG00000022783.
GeneIDi50525.
KEGGimmu:50525.
UCSCiuc007yjb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF173866 mRNA. Translation: AAF35831.1.
AK005732 mRNA. Translation: BAB24211.1.
AF486266 Genomic DNA. Translation: AAM64149.1.
CCDSiCCDS27988.1.
RefSeqiNP_056588.1. NM_015773.2.
UniGeneiMm.31701.

3D structure databases

ProteinModelPortaliQ9JLI7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206052. 1 interactor.
IntActiQ9JLI7. 1 interactor.
STRINGi10090.ENSMUSP00000023468.

PTM databases

PhosphoSitePlusiQ9JLI7.

Proteomic databases

MaxQBiQ9JLI7.
PaxDbiQ9JLI7.
PRIDEiQ9JLI7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023468; ENSMUSP00000023468; ENSMUSG00000022783.
GeneIDi50525.
KEGGimmu:50525.
UCSCiuc007yjb.2. mouse.

Organism-specific databases

CTDi50525.
MGIiMGI:1354388. Spag6.

Phylogenomic databases

eggNOGiKOG0166. Eukaryota.
COG5064. LUCA.
GeneTreeiENSGT00760000119094.
HOGENOMiHOG000257051.
HOVERGENiHBG055326.
InParanoidiQ9JLI7.
OMAiRVENYQP.
OrthoDBiEOG091G07CA.
PhylomeDBiQ9JLI7.
TreeFamiTF328894.

Miscellaneous databases

PROiQ9JLI7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022783.
CleanExiMM_SPAG6.
ExpressionAtlasiQ9JLI7. baseline and differential.
GenevisibleiQ9JLI7. MM.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR000357. HEAT.
[Graphical view]
PfamiPF00514. Arm. 4 hits.
PF02985. HEAT. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSPAG6_MOUSE
AccessioniPrimary (citable) accession number: Q9JLI7
Secondary accession number(s): Q8K461
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.