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Protein

Sperm-associated antigen 6

Gene

Spag6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Important for structural integrity of the central apparatus in the sperm tail and for flagellar motility.2 Publications

GO - Biological processi

  • sperm axoneme assembly Source: MGI
  • sperm motility Source: MGI
  • ventricular system development Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm-associated antigen 6
Alternative name(s):
Axoneme central apparatus protein
Protein PF16 homolog
Gene namesi
Name:Spag6
Synonyms:Pf16
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1354388. Spag6.

Subcellular locationi

GO - Cellular componenti

  • axonemal central apparatus Source: MGI
  • cytoplasm Source: UniProtKB-KW
  • microtubule Source: UniProtKB-KW
  • microtubule cytoskeleton Source: MGI
  • nucleus Source: MGI
  • sperm principal piece Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum, Microtubule

Pathology & Biotechi

Involvement in diseasei

Defects in Spag6 are a cause of hydrocephalus and of male infertility.

Disruption phenotypei

In null embryos the central apparatus is unstable leading to a loss, and presumably degradation of proteins to which SPAG6 normally binds, including SPAG16 AND SPAG17.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 507507Sperm-associated antigen 6PRO_0000072096Add
BLAST

Proteomic databases

MaxQBiQ9JLI7.
PaxDbiQ9JLI7.
PRIDEiQ9JLI7.

Expressioni

Tissue specificityi

Highly expressed in testis. Not detected in prostate, ovary, spleen, thymus, small intestine, colon and peripheral blood leukocytes.2 Publications

Gene expression databases

BgeeiQ9JLI7.
CleanExiMM_SPAG6.
ExpressionAtlasiQ9JLI7. baseline and differential.
GenevisibleiQ9JLI7. MM.

Interactioni

Subunit structurei

Interacts with SPAG16 AND SPAG17.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Spag17Q5S0033EBI-1783654,EBI-1783665

Protein-protein interaction databases

BioGridi206052. 1 interaction.
IntActiQ9JLI7. 1 interaction.
STRINGi10090.ENSMUSP00000023468.

Structurei

3D structure databases

ProteinModelPortaliQ9JLI7.
SMRiQ9JLI7. Positions 1-479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati31 – 7040ARM 1Add
BLAST
Repeati73 – 11240ARM 2Add
BLAST
Repeati115 – 15440ARM 3Add
BLAST
Repeati157 – 19640ARM 4Add
BLAST
Repeati199 – 23840ARM 5Add
BLAST
Repeati241 – 28040ARM 6Add
BLAST
Repeati325 – 36541ARM 7Add
BLAST
Repeati402 – 44140ARM 8Add
BLAST

Sequence similaritiesi

Contains 8 ARM repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0166. Eukaryota.
COG5064. LUCA.
GeneTreeiENSGT00760000119094.
HOGENOMiHOG000257051.
HOVERGENiHBG055326.
InParanoidiQ9JLI7.
OMAiRVENYQP.
OrthoDBiEOG7WMCJ9.
PhylomeDBiQ9JLI7.
TreeFamiTF328894.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR000357. HEAT.
[Graphical view]
PfamiPF00514. Arm. 4 hits.
PF02985. HEAT. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9JLI7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQRQVLQVF EQYQKARTQF VQMVAELATR PQNIETLQNA GVMSLLRPLL
60 70 80 90 100
LDVVPTIQQT AALALGRLAN YNDDLAEAVV KGDILPQLVY SLAEQNRFYK
110 120 130 140 150
KAAAFVLRAV GKHSPQLAQA IVDCGALDTL VICLEDFDPG VKEAAAWALG
160 170 180 190 200
YIARHNTELS QAVVDAGAIP LLVLCIQEPE IALKRIAASA LSDISKHSPE
210 220 230 240 250
LAQTVVDAGA IAHLAQMILN PDAKLKRQVL SALSQIAKHS VDLAEMVVEA
260 270 280 290 300
EIFPVVLTCL KDKDEYVKKN ACTLIREIAK HTPELSQLIV NAGGVAAVID
310 320 330 340 350
CIGSCKGNIR LPGIMMLGYV AAHSENLAMA VIISKGVPQL SICLSEEPED
360 370 380 390 400
HIKAAAAWAL GQLGRHTPEH ARAVAVTNTL PVLLSLYMSP ESSEDLQLKS
410 420 430 440 450
KKAIKNILQK CTYLPALEPF LYDAPPNILK HVVGQFSKVL PHDSKARRLF
460 470 480 490 500
VTSGGLKKVQ EIKAEPGSLL QEYINSINNC YPEEIVRYYS PGYSDTLLQR

VDSYQPL
Length:507
Mass (Da):55,269
Last modified:October 1, 2000 - v1
Checksum:iEFFAE719EC111285
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF173866 mRNA. Translation: AAF35831.1.
AK005732 mRNA. Translation: BAB24211.1.
AF486266 Genomic DNA. Translation: AAM64149.1.
CCDSiCCDS27988.1.
RefSeqiNP_056588.1. NM_015773.2.
UniGeneiMm.31701.

Genome annotation databases

EnsembliENSMUST00000023468; ENSMUSP00000023468; ENSMUSG00000022783.
GeneIDi50525.
KEGGimmu:50525.
UCSCiuc007yjb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF173866 mRNA. Translation: AAF35831.1.
AK005732 mRNA. Translation: BAB24211.1.
AF486266 Genomic DNA. Translation: AAM64149.1.
CCDSiCCDS27988.1.
RefSeqiNP_056588.1. NM_015773.2.
UniGeneiMm.31701.

3D structure databases

ProteinModelPortaliQ9JLI7.
SMRiQ9JLI7. Positions 1-479.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206052. 1 interaction.
IntActiQ9JLI7. 1 interaction.
STRINGi10090.ENSMUSP00000023468.

Proteomic databases

MaxQBiQ9JLI7.
PaxDbiQ9JLI7.
PRIDEiQ9JLI7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023468; ENSMUSP00000023468; ENSMUSG00000022783.
GeneIDi50525.
KEGGimmu:50525.
UCSCiuc007yjb.2. mouse.

Organism-specific databases

CTDi9576.
MGIiMGI:1354388. Spag6.

Phylogenomic databases

eggNOGiKOG0166. Eukaryota.
COG5064. LUCA.
GeneTreeiENSGT00760000119094.
HOGENOMiHOG000257051.
HOVERGENiHBG055326.
InParanoidiQ9JLI7.
OMAiRVENYQP.
OrthoDBiEOG7WMCJ9.
PhylomeDBiQ9JLI7.
TreeFamiTF328894.

Miscellaneous databases

PROiQ9JLI7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JLI7.
CleanExiMM_SPAG6.
ExpressionAtlasiQ9JLI7. baseline and differential.
GenevisibleiQ9JLI7. MM.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR000357. HEAT.
[Graphical view]
PfamiPF00514. Arm. 4 hits.
PF02985. HEAT. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sperm antigen 6 is the murine homologue of the Chlamydomonas reinhardtii central apparatus protein encoded by the PF16 locus."
    Sapiro R., Tarantino L.M., Velazquez F., Kiriakidou M., Hecht N.B., Bucan M., Strauss J.F. III
    Biol. Reprod. 62:511-518(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Tissue: Sperm.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  3. "Male infertility, impaired sperm motility, and hydrocephalus in mice deficient in sperm-associated antigen 6."
    Sapiro R., Kostetskii I., Olds-Clarke P., Gerton G.L., Radice G.L., Strauss J.F. III
    Mol. Cell. Biol. 22:6298-6305(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 42-96, FUNCTION, DISEASE.
    Strain: C57BL/6J.
    Tissue: Testis.
  4. "A sperm-associated WD repeat protein orthologous to Chlamydomonas PF20 associates with Spag6, the mammalian orthologue of Chlamydomonas PF16."
    Zhang Z., Sapiro R., Kapfhamer D., Bucan M., Bray J., Chennathukuzhi V., McNamara P., Curtis A., Zhang M., Blanchette-Mackie E.J., Strauss J.F. III
    Mol. Cell. Biol. 22:7993-8004(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SPAG16, TISSUE SPECIFICITY.
    Tissue: Sperm.
  5. "Dissecting the axoneme interactome: the mammalian orthologue of Chlamydomonas PF6 interacts with sperm-associated antigen 6, the mammalian orthologue of Chlamydomonas PF16."
    Zhang Z., Jones B.H., Tang W., Moss S.B., Wei Z., Ho C., Pollack M., Horowitz E., Bennett J., Baker M.E., Strauss J.F. III
    Mol. Cell. Proteomics 4:914-923(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SPAG17, DISRUPTION PHENOTYPE.
    Strain: C57BL/6J.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiSPAG6_MOUSE
AccessioniPrimary (citable) accession number: Q9JLI7
Secondary accession number(s): Q8K461
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.