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Protein

Selenocysteine lyase

Gene

Scly

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the decomposition of L-selenocysteine to L-alanine and elemental selenium.1 Publication

Catalytic activityi

L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor.1 Publication

Cofactori

pyridoxal 5'-phosphate1 Publication

Kineticsi

  1. KM=9.9 mM for L-selenocysteine1 Publication
  2. KM=8.6 mM for L-cysteine sulfinate1 Publication
  3. KM=5.2 mM for L-cysteine1 Publication
  1. Vmax=58 µmol/min/mg enzyme with L-selenocysteine as substrate1 Publication
  2. Vmax=0.45 µmol/min/mg enzyme with L-cysteine sulfinate as substrate1 Publication
  3. Vmax=0.0074 µmol/min/mg enzyme with L-cysteine as substrate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei375 – 3751S-selanylcysteine intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

  • lipid metabolic process Source: MGI
  • negative regulation of cellular response to oxidative stress Source: MGI
  • response to insulin Source: MGI
  • selenium compound metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi4.4.1.16. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Selenocysteine lyase (EC:4.4.1.16)
Short name:
mSCL
Gene namesi
Name:Scly
Synonyms:Scl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1355310. Scly.

Subcellular locationi

  • Cytoplasmcytosol 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 432432Selenocysteine lyasePRO_0000317013Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei117 – 1171PhosphoserineBy similarity
Modified residuei247 – 2471N6-(pyridoxal phosphate)lysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9JLI6.
MaxQBiQ9JLI6.
PaxDbiQ9JLI6.
PeptideAtlasiQ9JLI6.
PRIDEiQ9JLI6.

PTM databases

iPTMnetiQ9JLI6.
PhosphoSiteiQ9JLI6.

Expressioni

Tissue specificityi

Widely expressed. Present in liver, kidney, testis (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000026307.
CleanExiMM_SCLY.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiQ9JLI6. 2 interactions.
MINTiMINT-4130305.
STRINGi10090.ENSMUSP00000027532.

Structurei

3D structure databases

ProteinModelPortaliQ9JLI6.
SMRiQ9JLI6. Positions 18-430.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1549. Eukaryota.
COG1104. LUCA.
HOGENOMiHOG000017510.
HOVERGENiHBG003708.
InParanoidiQ9JLI6.
KOiK01763.
PhylomeDBiQ9JLI6.
TreeFamiTF313550.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR016454. Cysteine_dSase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF005572. NifS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JLI6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDAARNGALG SVESLPDRKV YMDYNATTPL EPEVIQAVTE AMKEAWGNPS
60 70 80 90 100
SSYVSGRKAK DIINAARASL AKMIGGKPQD IIFTSGGTES NNLVIHSMVR
110 120 130 140 150
CFHEQQTLKG NMVDQHSPEE GTRPHFITCT VEHDSIRLPL EHLVENQMAE
160 170 180 190 200
VTFVPVSKVN GQAEVEDILA AVRPTTCLVT IMLANNETGV IMPVSEISRR
210 220 230 240 250
IKALNQIRAA SGLPRVLVHT DAAQALGKRR VDVEDLGVDF LTIVGHKFYG
260 270 280 290 300
PRIGALYVRG VGKLTPLYPM LFGGGQERNF RPGTENTPMI AGLGKAADLV
310 320 330 340 350
SENCETYEAH MRDIRDYLEE RLEAEFGKRI HLNSRFPGVE RLPNTCNFSI
360 370 380 390 400
QGSQLQGYTV LAQCRTLLAS VGASCHSNHE DRPSPVLLSC GIPVDVARNA
410 420 430
VRLSVGRGTT RADVDLIVQD LKQAVAQLEG RL
Length:432
Mass (Da):47,174
Last modified:October 1, 2000 - v1
Checksum:iAFC1800A1BC349D4
GO
Isoform 2 (identifier: Q9JLI6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     284-432: Missing.

Note: No experimental confirmation available.
Show »
Length:283
Mass (Da):30,858
Checksum:iEA9FBEED52F31550
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti146 – 1461N → S in BAC29465 (PubMed:16141072).Curated
Sequence conflicti278 – 2781R → W in BAC29465 (PubMed:16141072).Curated
Sequence conflicti278 – 2781R → W in BAC29563 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei284 – 432149Missing in isoform 2. 1 PublicationVSP_030855Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175407 mRNA. Translation: AAF36812.1.
AK036533 mRNA. Translation: BAC29465.1.
AK036750 mRNA. Translation: BAC29563.1.
BC019879 mRNA. Translation: AAH19879.1.
BC021389 mRNA. Translation: AAH21389.1.
CCDSiCCDS15159.1. [Q9JLI6-1]
RefSeqiNP_057926.2. NM_016717.3.
UniGeneiMm.25724.

Genome annotation databases

GeneIDi50880.
KEGGimmu:50880.
UCSCiuc007cae.1. mouse. [Q9JLI6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175407 mRNA. Translation: AAF36812.1.
AK036533 mRNA. Translation: BAC29465.1.
AK036750 mRNA. Translation: BAC29563.1.
BC019879 mRNA. Translation: AAH19879.1.
BC021389 mRNA. Translation: AAH21389.1.
CCDSiCCDS15159.1. [Q9JLI6-1]
RefSeqiNP_057926.2. NM_016717.3.
UniGeneiMm.25724.

3D structure databases

ProteinModelPortaliQ9JLI6.
SMRiQ9JLI6. Positions 18-430.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JLI6. 2 interactions.
MINTiMINT-4130305.
STRINGi10090.ENSMUSP00000027532.

PTM databases

iPTMnetiQ9JLI6.
PhosphoSiteiQ9JLI6.

Proteomic databases

EPDiQ9JLI6.
MaxQBiQ9JLI6.
PaxDbiQ9JLI6.
PeptideAtlasiQ9JLI6.
PRIDEiQ9JLI6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi50880.
KEGGimmu:50880.
UCSCiuc007cae.1. mouse. [Q9JLI6-1]

Organism-specific databases

CTDi51540.
MGIiMGI:1355310. Scly.

Phylogenomic databases

eggNOGiKOG1549. Eukaryota.
COG1104. LUCA.
HOGENOMiHOG000017510.
HOVERGENiHBG003708.
InParanoidiQ9JLI6.
KOiK01763.
PhylomeDBiQ9JLI6.
TreeFamiTF313550.

Enzyme and pathway databases

BRENDAi4.4.1.16. 3474.

Miscellaneous databases

ChiTaRSiScly. mouse.
PROiQ9JLI6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026307.
CleanExiMM_SCLY.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR016454. Cysteine_dSase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF005572. NifS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSCLY_MOUSE
AccessioniPrimary (citable) accession number: Q9JLI6
Secondary accession number(s): Q8CB57, Q8CB94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.