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Q9JLI4

- NCOA6_RAT

UniProt

Q9JLI4 - NCOA6_RAT

Protein

Nuclear receptor coactivator 6

Gene

Ncoa6

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 2 (12 Feb 2003)
      Previous versions | rss
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    Functioni

    Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivate expression in an agonist- and AF2-dependent manner. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway By similarity.By similarity

    GO - Molecular functioni

    1. ligand-dependent nuclear receptor binding Source: RGD
    2. ligand-dependent nuclear receptor transcription coactivator activity Source: RGD
    3. protein binding Source: IntAct
    4. protein homodimerization activity Source: RGD
    5. thyroid hormone receptor binding Source: UniProtKB

    GO - Biological processi

    1. myeloid cell differentiation Source: UniProtKB
    2. positive regulation of transcription, DNA-templated Source: RGD
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear receptor coactivator 6
    Alternative name(s):
    Activating signal cointegrator 2
    Short name:
    ASC-2
    Amplified in breast cancer protein 3
    Cancer-amplified transcriptional coactivator ASC-2
    Nuclear receptor coactivator RAP250
    Short name:
    NRC
    Nuclear receptor-activating protein, 250 kDa
    Peroxisome proliferator-activated receptor-interacting protein
    Short name:
    PPAR-interacting protein
    Short name:
    PRIP
    Thyroid hormone receptor-binding protein
    Gene namesi
    Name:Ncoa6
    Synonyms:Aib3, Rap250, Trbp
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620111. Ncoa6.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. protein complex Source: RGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi174 – 1785LVNLL → AVNAA: Abolishes interaction with nuclear receptors. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 418›418Nuclear receptor coactivator 6PRO_0000094415Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei171 – 1711PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9JLI4.
    PRIDEiQ9JLI4.

    PTM databases

    PhosphoSiteiQ9JLI4.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9JLI4.

    Interactioni

    Subunit structurei

    Monomer and homodimer. Interacts in vitro with the basal transcription factors GTF2A and TBP, suggesting an autonomous transactivation function. Interacts with NCOA1, CRSP3, RBM14, the histone acetyltransferase proteins EP300 and CREBBP, and with methyltransferase proteins NCOA6IP and PRMT2 By similarity. Component of the MLL2/3 complex (also named ASCOM complex), at least composed of KMT2D/MLL2 or KMT2C/MLL3, ASH2L, RBBP5, WDR5, NCOA6, DPY30, KDM6A, PAXIP1/PTIP, PAGR1 and alpha- and beta-tubulin By similarity. Interacts with ZNF335; may enhance ligand-dependent transcriptional activation by nuclear hormone receptors By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ESR1P033722EBI-286271,EBI-78473From a different organism.
    GHRP109122EBI-286271,EBI-286316From a different organism.
    RXRAP197932EBI-286271,EBI-78598From a different organism.
    THRAP108272EBI-286271,EBI-286285From a different organism.
    VDRP114732EBI-286271,EBI-286357From a different organism.

    Protein-protein interaction databases

    IntActiQ9JLI4. 9 interactions.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni‹1 – 418›418NCOA1-binding regionBy similarityAdd
    BLAST
    Regioni‹1 – 352›352CREBBP-binding regionBy similarityAdd
    BLAST
    Regioni‹1 – 215›215TBP/GTF2A-binding regionBy similarityAdd
    BLAST
    Regioni60 – 214155NCOA6IP-binding regionBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi174 – 1785LXXLL motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi200 – 2056Poly-Lys
    Compositional biasi301 – 3088Poly-Pro
    Compositional biasi310 – 32718Poly-GlnAdd
    BLAST
    Compositional biasi328 – 3347Poly-Met

    Domaini

    Contains one Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif, which is essential for the association with nuclear receptors.

    Phylogenomic databases

    eggNOGiNOG12793.
    HOGENOMiHOG000113745.
    InParanoidiQ9JLI4.

    Family and domain databases

    InterProiIPR026638. NCOA6.
    [Graphical view]
    PANTHERiPTHR15690. PTHR15690. 1 hit.

    Sequencei

    Sequence statusi: Fragment.

    Q9JLI4-1 [UniParc]FASTAAdd to Basket

    « Hide

    EQIMTNQMQG NKAQFNSQNQ SNVMPGPAQI MRGPTPNMQG NMVQFTGQMS    50
    GQMLPQQGPV SNSPSQVMGI QGQVLRPPGP SPHMAQQHTD PATTANNDVN 100
    LSQMMPDVSM QQTSMVPPHV QSMQGNSASG SHFSGHGVSF NAPFGGAPNG 150
    TQMSCGQNPG FPVNKDVTLT SPLLVNLLQS DISAGHFGVN NKQNNTNANK 200
    QKKKKPPRKK KNCHQDLNTP DSRPAGLEEV DQQSLPGEQG INLDNTGPKL 250
    PDFSNRPPGY PTQPVEQRPL QQMPPQLMQH VAPPPQPPQQ QPQPQLPQQQ 300
    PQPQPPPPSQ PQSQQQQQQQ QQQQQQQMMM MLMMQQDPKS IRLPVSQNVH 350
    PPRGPLNPDS QRVPMQQSGN VPVMVSLQGP ASVPPSPDKQ RMPMPVNTPL 400
    GSNSRKMVYQ ESPQNSSS 418
    Length:418
    Mass (Da):45,574
    Last modified:February 12, 2003 - v2
    Checksum:iA90D7C4111C67CAE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF228043 mRNA. Translation: AAF76422.1.
    AF176351 mRNA. Translation: AAF71830.1.
    UniGeneiRn.234544.
    Rn.9077.

    Genome annotation databases

    UCSCiRGD:620111. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF228043 mRNA. Translation: AAF76422.1 .
    AF176351 mRNA. Translation: AAF71830.1 .
    UniGenei Rn.234544.
    Rn.9077.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9JLI4. 9 interactions.

    PTM databases

    PhosphoSitei Q9JLI4.

    Proteomic databases

    PaxDbi Q9JLI4.
    PRIDEi Q9JLI4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:620111. rat.

    Organism-specific databases

    RGDi 620111. Ncoa6.

    Phylogenomic databases

    eggNOGi NOG12793.
    HOGENOMi HOG000113745.
    InParanoidi Q9JLI4.

    Miscellaneous databases

    PROi Q9JLI4.

    Gene expression databases

    Genevestigatori Q9JLI4.

    Family and domain databases

    InterProi IPR026638. NCOA6.
    [Graphical view ]
    PANTHERi PTHR15690. PTHR15690. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Thyroid hormone receptor-binding protein, an LXXLL motif-containing protein, functions as a general coactivator."
      Ko L., Cardona G.R., Chin W.W.
      Proc. Natl. Acad. Sci. U.S.A. 97:6212-6217(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-285, INTERACTION WITH THRB.
      Tissue: Pituitary.
    2. "A new family of nuclear receptor coregulators that integrates nuclear receptor signaling through CBP."
      Mahajan M.A., Samuels H.H.
      Mol. Cell. Biol. 20:5048-5063(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 135-418, INTERACTION WITH THRA; THRB; RAR; RXR; ESR; VDR; NR3C1, MUTAGENESIS OF 174-LEU--LEU-178.
      Strain: Sprague-Dawley.
      Tissue: Pituitary.

    Entry informationi

    Entry nameiNCOA6_RAT
    AccessioniPrimary (citable) accession number: Q9JLI4
    Secondary accession number(s): Q9JIH6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 12, 2003
    Last sequence update: February 12, 2003
    Last modified: October 1, 2014
    This is version 84 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    External Data

    Dasty 3