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Q9JLI4 (NCOA6_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Nuclear receptor coactivator 6
Alternative name(s):
Activating signal cointegrator 2
Short name=ASC-2
Amplified in breast cancer protein 3
Cancer-amplified transcriptional coactivator ASC-2
Nuclear receptor coactivator RAP250
Short name=NRC
Nuclear receptor-activating protein, 250 kDa
Peroxisome proliferator-activated receptor-interacting protein
Short name=PPAR-interacting protein
Short name=PRIP
Thyroid hormone receptor-binding protein
Gene names
Name:Ncoa6
Synonyms:Aib3, Rap250, Trbp
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length418 AA.
Sequence statusFragment.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivate expression in an agonist- and AF2-dependent manner. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway By similarity.

Subunit structure

Monomer and homodimer. Interacts in vitro with the basal transcription factors GTF2A and TBP, suggesting an autonomous transactivation function. Interacts with NCOA1, CRSP3, RBM14, the histone acetyltransferase proteins EP300 and CREBBP, and with methyltransferase proteins NCOA6IP and PRMT2 By similarity. Component of the MLL2/3 complex (also named ASCOM complex), at least composed of KMT2D/MLL2 or KMT2C/MLL3, ASH2L, RBBP5, WDR5, NCOA6, DPY30, KDM6A, PAXIP1/PTIP, PAGR1 and alpha- and beta-tubulin By similarity. Interacts with ZNF335; may enhance ligand-dependent transcriptional activation by nuclear hormone receptors By similarity. Ref.1 Ref.2

Subcellular location

Nucleus.

Domain

Contains one Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif, which is essential for the association with nuclear receptors.

Post-translational modification

Phosphorylated By similarity.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ESR1P033722EBI-286271,EBI-78473From a different organism.
GHRP109122EBI-286271,EBI-286316From a different organism.
RXRAP197932EBI-286271,EBI-78598From a different organism.
THRAP108272EBI-286271,EBI-286285From a different organism.
VDRP114732EBI-286271,EBI-286357From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 418›418Nuclear receptor coactivator 6
PRO_0000094415

Regions

Region‹1 – 418›418NCOA1-binding region By similarity
Region‹1 – 352›352CREBBP-binding region By similarity
Region‹1 – 215›215TBP/GTF2A-binding region By similarity
Region60 – 214155NCOA6IP-binding region By similarity
Motif174 – 1785LXXLL motif
Compositional bias200 – 2056Poly-Lys
Compositional bias301 – 3088Poly-Pro
Compositional bias310 – 32718Poly-Gln
Compositional bias328 – 3347Poly-Met

Amino acid modifications

Modified residue1711Phosphoserine By similarity

Experimental info

Mutagenesis174 – 1785LVNLL → AVNAA: Abolishes interaction with nuclear receptors. Ref.2
Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
Q9JLI4 [UniParc].

Last modified February 12, 2003. Version 2.
Checksum: A90D7C4111C67CAE

FASTA41845,574
        10         20         30         40         50         60 
EQIMTNQMQG NKAQFNSQNQ SNVMPGPAQI MRGPTPNMQG NMVQFTGQMS GQMLPQQGPV 

        70         80         90        100        110        120 
SNSPSQVMGI QGQVLRPPGP SPHMAQQHTD PATTANNDVN LSQMMPDVSM QQTSMVPPHV 

       130        140        150        160        170        180 
QSMQGNSASG SHFSGHGVSF NAPFGGAPNG TQMSCGQNPG FPVNKDVTLT SPLLVNLLQS 

       190        200        210        220        230        240 
DISAGHFGVN NKQNNTNANK QKKKKPPRKK KNCHQDLNTP DSRPAGLEEV DQQSLPGEQG 

       250        260        270        280        290        300 
INLDNTGPKL PDFSNRPPGY PTQPVEQRPL QQMPPQLMQH VAPPPQPPQQ QPQPQLPQQQ 

       310        320        330        340        350        360 
PQPQPPPPSQ PQSQQQQQQQ QQQQQQQMMM MLMMQQDPKS IRLPVSQNVH PPRGPLNPDS 

       370        380        390        400        410 
QRVPMQQSGN VPVMVSLQGP ASVPPSPDKQ RMPMPVNTPL GSNSRKMVYQ ESPQNSSS 

« Hide

References

[1]"Thyroid hormone receptor-binding protein, an LXXLL motif-containing protein, functions as a general coactivator."
Ko L., Cardona G.R., Chin W.W.
Proc. Natl. Acad. Sci. U.S.A. 97:6212-6217(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-285, INTERACTION WITH THRB.
Tissue: Pituitary.
[2]"A new family of nuclear receptor coregulators that integrates nuclear receptor signaling through CBP."
Mahajan M.A., Samuels H.H.
Mol. Cell. Biol. 20:5048-5063(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 135-418, INTERACTION WITH THRA; THRB; RAR; RXR; ESR; VDR; NR3C1, MUTAGENESIS OF 174-LEU--LEU-178.
Strain: Sprague-Dawley.
Tissue: Pituitary.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF228043 mRNA. Translation: AAF76422.1.
AF176351 mRNA. Translation: AAF71830.1.
UniGeneRn.234544.
Rn.9077.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9JLI4. 9 interactions.

PTM databases

PhosphoSiteQ9JLI4.

Proteomic databases

PaxDbQ9JLI4.
PRIDEQ9JLI4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:620111. rat.

Organism-specific databases

RGD620111. Ncoa6.

Phylogenomic databases

eggNOGNOG12793.
HOGENOMHOG000113745.
InParanoidQ9JLI4.

Gene expression databases

GenevestigatorQ9JLI4.

Family and domain databases

InterProIPR026638. NCOA6.
[Graphical view]
PANTHERPTHR15690. PTHR15690. 1 hit.
ProtoNetSearch...

Other

PROQ9JLI4.

Entry information

Entry nameNCOA6_RAT
AccessionPrimary (citable) accession number: Q9JLI4
Secondary accession number(s): Q9JIH6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: February 12, 2003
Last modified: February 19, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program