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Protein

CDK5 regulatory subunit-associated protein 3

Gene

Cdk5rap3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable tumor suppressor initially identified as a CDK5R1 interactor controlling cell proliferation. Negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation. Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint. Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation. May play a role in the unfolded protein response, mediating the ufmylation of multiple proteins in response to endoplasmic reticulum stress. May also play a role in the rupture of the nuclear envelope during apoptosis. May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1.By similarity

GO - Molecular functioni

  • protein complex binding Source: RGD
  • ubiquitin-like protein ligase binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
CDK5 regulatory subunit-associated protein 3Curated
Alternative name(s):
CDK5 activator-binding protein C531 Publication
Gene namesi
Name:Cdk5rap3Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620002. Cdk5rap3.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

  • Note: Colocalizes and associates with microtubules.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504CDK5 regulatory subunit-associated protein 3PRO_0000220519Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki448 – 448Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

May be phosphorylated by CDK5.1 Publication
May be ufmylated.By similarity
Ubiquitinated. Probably triggers proteasomal degradation and is negatively regulated by UFL1.By similarity
Cleaved by caspases early during apoptosis, the resulting peptides may play a role in rupture of the nuclear envelope.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9JLH7.
PRIDEiQ9JLH7.

Expressioni

Tissue specificityi

Expressed in vascular endothelium. Up-regulated in failing heart. Highly expressed in the ventricular section in subacute and chronic ischemic heart failure.1 Publication

Interactioni

Subunit structurei

Interacts with CDK5R1; competes with CDK5RAP1 and CDK5RAP2 (PubMed:10721722). Interacts with RELA. Interacts with CHEK1; may negatively regulate CHEK1 and thereby stimulate entry into mitosis. Interacts with CDKN2A/ARF and MDM2; forms a ternary complex involved in regulation of p53/TP53. Interacts with UFL1; the interaction is direct. Interacts with DDRGK1. Interacts with MAPK14. Interacts with CCNB1. Interacts with TUBG1; may regulate CDK5RAP3 in mitotic G2/M transition checkpoint (By similarity).By similarity1 Publication

GO - Molecular functioni

  • protein complex binding Source: RGD
  • ubiquitin-like protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi249477. 1 interaction.
IntActiQ9JLH7. 3 interactions.
MINTiMINT-4568319.
STRINGi10116.ENSRNOP00000065240.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni268 – 504237Required for interaction with UFL1 and mediates interaction with CHEK1By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the CDK5RAP3 family.Curated

Phylogenomic databases

eggNOGiKOG2607. Eukaryota.
ENOG410XSWB. LUCA.
HOVERGENiHBG050978.
InParanoidiQ9JLH7.
PhylomeDBiQ9JLH7.

Family and domain databases

InterProiIPR008491. DUF773.
[Graphical view]
PANTHERiPTHR14894. PTHR14894. 1 hit.
PfamiPF05600. DUF773. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JLH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDHQHVPID IQTSKLLDWL VDRRHCNLKW QSLVLTIREK INTAIQDMPE
60 70 80 90 100
SQEIAQLLSG SYIHYFHCLR IVDLLKGTEA STKNIFGRYS SQRMKDWQEI
110 120 130 140 150
ISLYEKDNTY LVELSSLLVR NVNYEIPSLK KQIAKCQQLQ QDYSRKEEEG
160 170 180 190 200
QAGAAEMREQ FYHSCKQYGI TGDNVRRELL ALVKDLPSQL AEIGAGAQSL
210 220 230 240 250
GEAIDLYQAC VEFVCDSPTE QVLPMLRYVQ PKGNSTVYEW RTGTEPSVVE
260 270 280 290 300
RPQLEDPPEQ VQEDEIDWGD FGLEAVSDSG NIISAETPGI DWGISLESES
310 320 330 340 350
KDAGADKIDW GDNAVASEIT VLETGTEAPE GVARGSDALT LLEYPETRNQ
360 370 380 390 400
FIDELMELEI FLSQRAVEMS EEADILSVSQ FQLAPAILQG QTKEKMLSLV
410 420 430 440 450
STLQHLIGQL TSLDLQHLFM ILASPRYVDR VTELLQQKLK QSQLLALKKD
460 470 480 490 500
LMVEKQQEAL QEQAALEPKL DLLLEKTREL QKLIEADISK RYNGRPVNLM

GTSV
Length:504
Mass (Da):57,045
Last modified:October 1, 2000 - v1
Checksum:i5283EF7DA2D7042E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177476 mRNA. Translation: AAF60222.1.
UniGeneiRn.3727.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177476 mRNA. Translation: AAF60222.1.
UniGeneiRn.3727.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249477. 1 interaction.
IntActiQ9JLH7. 3 interactions.
MINTiMINT-4568319.
STRINGi10116.ENSRNOP00000065240.

Proteomic databases

PaxDbiQ9JLH7.
PRIDEiQ9JLH7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi620002. Cdk5rap3.

Phylogenomic databases

eggNOGiKOG2607. Eukaryota.
ENOG410XSWB. LUCA.
HOVERGENiHBG050978.
InParanoidiQ9JLH7.
PhylomeDBiQ9JLH7.

Miscellaneous databases

PROiQ9JLH7.

Family and domain databases

InterProiIPR008491. DUF773.
[Graphical view]
PANTHERiPTHR14894. PTHR14894. 1 hit.
PfamiPF05600. DUF773. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of three novel neuronal Cdk5 activator binding proteins."
    Ching Y.-P., Qi Z., Wang J.H.
    Gene 242:285-294(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CDK5R1, PHOSPHORYLATION.
  2. "A novel gene IC53 stimulates ECV304 cell proliferation and is upregulated in failing heart."
    Chen J., Liu B., Liu Y.Q., Han Y., Yu H., Zhang Y., Lu L., Zhen Y., Hui R.T.
    Biochem. Biophys. Res. Commun. 294:161-166(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiCK5P3_RAT
AccessioniPrimary (citable) accession number: Q9JLH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: October 1, 2000
Last modified: July 6, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.