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Protein

CDK5 regulatory subunit-associated protein 1

Gene

Cdk5rap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable regulator of CDK5 activity. May inhibit CDK5 function via its interaction with CDK5R1.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi106Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi142Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi180Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi255Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi259Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi262Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1

GO - Molecular functioni

GO - Biological processi

  • brain development Source: RGD
  • cell proliferation Source: UniProtKB
  • mitochondrial tRNA modification Source: RGD
  • negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
  • positive regulation of mitochondrial translation Source: RGD
  • positive regulation of translational fidelity Source: RGD
  • regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
  • regulation of neuron differentiation Source: RGD
  • regulation of protein kinase activity Source: RGD

Keywordsi

Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
CDK5 regulatory subunit-associated protein 1
Alternative name(s):
CDK5 activator-binding protein C42
Gene namesi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi708387 Cdk5rap1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001417661 – 586CDK5 regulatory subunit-associated protein 1Add BLAST586

Proteomic databases

PaxDbiQ9JLH6
PRIDEiQ9JLH6

Expressioni

Gene expression databases

BgeeiENSRNOG00000015696
GenevisibleiQ9JLH6 RN

Interactioni

Subunit structurei

Interacts with CDK5R1 (p35 form). CDK5RAP1, CDK5RAP2 and CDK5RAP3 show competitive binding to CDK5R1. Forms a complex with CDK5R1 and CDK5.2 Publications

Protein-protein interaction databases

BioGridi251627, 1 interactor
IntActiQ9JLH6, 2 interactors
STRINGi10116.ENSRNOP00000021418

Structurei

3D structure databases

ProteinModelPortaliQ9JLH6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini97 – 217MTTase N-terminalAdd BLAST121
Domaini498 – 573TRAMAdd BLAST76

Domaini

The C-terminal part (475-586) is necessary for the interaction with CDK5R1, while the N-terminal part (1-168) is required for inhibiting the activity of the CDK5 kinase.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2492 Eukaryota
COG0621 LUCA
GeneTreeiENSGT00530000063447
HOGENOMiHOG000224767
HOVERGENiHBG050975
InParanoidiQ9JLH6
OMAiKVCEHFH
OrthoDBiEOG091G07M5
PhylomeDBiQ9JLH6
TreeFamiTF101033

Family and domain databases

Gene3Di3.40.50.12160, 1 hit
3.80.30.20, 1 hit
HAMAPiMF_01864 tRNA_metthiotr_MiaB, 1 hit
InterProiView protein in InterPro
IPR006638 Elp3/MiaB/NifB
IPR005839 Methylthiotransferase
IPR020612 Methylthiotransferase_CS
IPR013848 Methylthiotransferase_N
IPR038135 Methylthiotransferase_N_sf
IPR006463 MiaB_methiolase
IPR007197 rSAM
IPR023404 rSAM_horseshoe
IPR002792 TRAM_dom
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PF01938 TRAM, 1 hit
PF00919 UPF0004, 1 hit
SFLDiSFLDF00273 (dimethylallyl)adenosine_tRNA_, 1 hit
SFLDG01061 methylthiotransferase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00089 TIGR00089, 1 hit
PROSITEiView protein in PROSITE
PS51449 MTTASE_N, 1 hit
PS01278 MTTASE_RADICAL, 1 hit
PS50926 TRAM, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JLH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPLQRVFRA QRLSAPLTSM CWVLLRTFRA HNSTSCPDPE GKSSEGVQKD
60 70 80 90 100
FSSRLATGPT FQHFLRSASV PQEKPSSPEV EDPPPYLSGD ELLGRQRKVY
110 120 130 140 150
LETYGCQMNV NDTEIAWSIL QKSGYLRTSN LQEADVILLV TCSIREKAEQ
160 170 180 190 200
TIWNRLHQLK VLKAKRPRSR VPLRIGILGC MAERLKGEIL NREKMVDLLA
210 220 230 240 250
GPDAYRDLPR LLAVVESGQQ AANVLLSLDE TYADIMPVQT SPSATSAFVS
260 270 280 290 300
IMRGCDNMCS YCIVPFTRGR ERSRPVASIL DEVRKLSEQG LKEVTLLGQN
310 320 330 340 350
VNSFRDNSEV QFSSTGSANL SRGFTTNYKP KQGGLRFSHL LDQVSRIDPE
360 370 380 390 400
MRIRFTSPHP KDFPDEVLQL IRERHNICKQ IHLPAQSGSS RVLEAMRRGY
410 420 430 440 450
SREAYVALVH HIREAIPGVG LSSDFITGFC GETEDDHLQT VSLLREVQYN
460 470 480 490 500
TGFLFAYSMR QKTRAYHRLK DDVPEEVKLR RLEELITVFR EEASKVNATS
510 520 530 540 550
VGCTQLVLVE GFSKRSTTDL CGRNDANLKV IFPDAEVEDI TDPGLKVRAQ
560 570 580
PGDYVLVKII SASSQTLKGH ILCRTTMKDS SMNCLT
Length:586
Mass (Da):65,866
Last modified:October 1, 2000 - v1
Checksum:i95AC432AA3FF552C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177477 mRNA Translation: AAF60223.1
BC066666 mRNA Translation: AAH66666.1
RefSeqiNP_663773.1, NM_145721.2
XP_006235369.1, XM_006235307.2
UniGeneiRn.203271

Genome annotation databases

EnsembliENSRNOT00000021418; ENSRNOP00000021418; ENSRNOG00000015696
GeneIDi252827
KEGGirno:252827

Similar proteinsi

Entry informationi

Entry nameiCK5P1_RAT
AccessioniPrimary (citable) accession number: Q9JLH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health