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Protein

CDK5 regulatory subunit-associated protein 1

Gene

Cdk5rap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable regulator of CDK5 activity. May inhibit CDK5 function via its interaction with CDK5R1.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi106 – 1061Iron-sulfur (4Fe-4S)By similarity
Metal bindingi142 – 1421Iron-sulfur (4Fe-4S)By similarity
Metal bindingi180 – 1801Iron-sulfur (4Fe-4S)By similarity
Metal bindingi255 – 2551Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi259 – 2591Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi262 – 2621Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein complex binding Source: RGD
  • protein kinase regulator activity Source: RGD
  • transferase activity Source: InterPro

GO - Biological processi

  • brain development Source: RGD
  • cell proliferation Source: UniProtKB
  • negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
  • regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
  • regulation of neuron differentiation Source: RGD
  • regulation of protein kinase activity Source: RGD
  • tRNA modification Source: InterPro
Complete GO annotation...

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
CDK5 regulatory subunit-associated protein 1
Alternative name(s):
CDK5 activator-binding protein C42
Gene namesi
Name:Cdk5rap1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi708387. Cdk5rap1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 586586CDK5 regulatory subunit-associated protein 1PRO_0000141766Add
BLAST

Proteomic databases

PaxDbiQ9JLH6.
PRIDEiQ9JLH6.

Expressioni

Gene expression databases

GenevisibleiQ9JLH6. RN.

Interactioni

Subunit structurei

Interacts with CDK5R1 (p35 form). CDK5RAP1, CDK5RAP2 and CDK5RAP3 show competitive binding to CDK5R1. Forms a complex with CDK5R1 and CDK5.2 Publications

GO - Molecular functioni

  • protein complex binding Source: RGD

Protein-protein interaction databases

BioGridi251627. 1 interaction.
IntActiQ9JLH6. 2 interactions.
STRINGi10116.ENSRNOP00000021418.

Structurei

3D structure databases

ProteinModelPortaliQ9JLH6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini97 – 217121MTTase N-terminalAdd
BLAST
Domaini498 – 57376TRAMAdd
BLAST

Domaini

The C-terminal part (475-586) is necessary for the interaction with CDK5R1, while the N-terminal part (1-168) is required for inhibiting the activity of the CDK5 kinase.

Sequence similaritiesi

Contains 1 MTTase N-terminal domain.Curated
Contains 1 TRAM domain.Curated

Phylogenomic databases

eggNOGiKOG2492. Eukaryota.
COG0621. LUCA.
GeneTreeiENSGT00530000063447.
HOGENOMiHOG000224767.
HOVERGENiHBG050975.
InParanoidiQ9JLH6.
OMAiCQMNFSD.
OrthoDBiEOG744T8N.
PhylomeDBiQ9JLH6.
TreeFamiTF101033.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 2 hits.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JLH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPLQRVFRA QRLSAPLTSM CWVLLRTFRA HNSTSCPDPE GKSSEGVQKD
60 70 80 90 100
FSSRLATGPT FQHFLRSASV PQEKPSSPEV EDPPPYLSGD ELLGRQRKVY
110 120 130 140 150
LETYGCQMNV NDTEIAWSIL QKSGYLRTSN LQEADVILLV TCSIREKAEQ
160 170 180 190 200
TIWNRLHQLK VLKAKRPRSR VPLRIGILGC MAERLKGEIL NREKMVDLLA
210 220 230 240 250
GPDAYRDLPR LLAVVESGQQ AANVLLSLDE TYADIMPVQT SPSATSAFVS
260 270 280 290 300
IMRGCDNMCS YCIVPFTRGR ERSRPVASIL DEVRKLSEQG LKEVTLLGQN
310 320 330 340 350
VNSFRDNSEV QFSSTGSANL SRGFTTNYKP KQGGLRFSHL LDQVSRIDPE
360 370 380 390 400
MRIRFTSPHP KDFPDEVLQL IRERHNICKQ IHLPAQSGSS RVLEAMRRGY
410 420 430 440 450
SREAYVALVH HIREAIPGVG LSSDFITGFC GETEDDHLQT VSLLREVQYN
460 470 480 490 500
TGFLFAYSMR QKTRAYHRLK DDVPEEVKLR RLEELITVFR EEASKVNATS
510 520 530 540 550
VGCTQLVLVE GFSKRSTTDL CGRNDANLKV IFPDAEVEDI TDPGLKVRAQ
560 570 580
PGDYVLVKII SASSQTLKGH ILCRTTMKDS SMNCLT
Length:586
Mass (Da):65,866
Last modified:October 1, 2000 - v1
Checksum:i95AC432AA3FF552C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177477 mRNA. Translation: AAF60223.1.
BC066666 mRNA. Translation: AAH66666.1.
RefSeqiNP_663773.1. NM_145721.2.
XP_006235369.1. XM_006235307.2.
XP_008760524.1. XM_008762302.1.
UniGeneiRn.203271.

Genome annotation databases

EnsembliENSRNOT00000021418; ENSRNOP00000021418; ENSRNOG00000015696.
GeneIDi252827.
KEGGirno:252827.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177477 mRNA. Translation: AAF60223.1.
BC066666 mRNA. Translation: AAH66666.1.
RefSeqiNP_663773.1. NM_145721.2.
XP_006235369.1. XM_006235307.2.
XP_008760524.1. XM_008762302.1.
UniGeneiRn.203271.

3D structure databases

ProteinModelPortaliQ9JLH6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251627. 1 interaction.
IntActiQ9JLH6. 2 interactions.
STRINGi10116.ENSRNOP00000021418.

Proteomic databases

PaxDbiQ9JLH6.
PRIDEiQ9JLH6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021418; ENSRNOP00000021418; ENSRNOG00000015696.
GeneIDi252827.
KEGGirno:252827.

Organism-specific databases

CTDi51654.
RGDi708387. Cdk5rap1.

Phylogenomic databases

eggNOGiKOG2492. Eukaryota.
COG0621. LUCA.
GeneTreeiENSGT00530000063447.
HOGENOMiHOG000224767.
HOVERGENiHBG050975.
InParanoidiQ9JLH6.
OMAiCQMNFSD.
OrthoDBiEOG744T8N.
PhylomeDBiQ9JLH6.
TreeFamiTF101033.

Miscellaneous databases

NextBioi623881.
PROiQ9JLH6.

Gene expression databases

GenevisibleiQ9JLH6. RN.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 2 hits.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of three novel neuronal Cdk5 activator binding proteins."
    Ching Y.-P., Qi Z., Wang J.H.
    Gene 242:285-294(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CDK5R1.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  3. "Identification of a neuronal Cdk5 activator-binding protein as Cdk5 inhibitor."
    Ching Y.-P., Pang A.S.H., Lam W.-H., Qi R.Z., Wang J.H.
    J. Biol. Chem. 277:15237-15240(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CDK5R1.

Entry informationi

Entry nameiCK5P1_RAT
AccessioniPrimary (citable) accession number: Q9JLH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: October 1, 2000
Last modified: May 11, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.