Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9JLF7 (TLR5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Toll-like receptor 5
Gene names
Name:Tlr5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length859 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response By similarity.

Subunit structure

Homodimer both in the absence and presence of ligand. Binds MYD88 via their respective TIR domains By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein By similarity.

Tissue specificity

Highly expressed in liver. Detected in lung and at very low levels in most other tissues.

Post-translational modification

Phosphorylated at Tyr-799 upon flagellin binding; required for signaling By similarity.

Polymorphism

The TLR5 gene lies in a locus that is associated with susceptibility to Salmonella. Inbred strains of mice can be classified into 3 categories according to their resistance to infection with S.typhimurium: susceptible (BALB/c, C57BL/6, C3H/He), intermediate (DBA/2, C75L) and resistant (A, CBA). The strain MOLF/Ei is highly susceptible to the infection, has an unique TLR5 haplotype and a lower expression of TRL5.

Sequence similarities

Belongs to the Toll-like receptor family.

Contains 16 LRR (leucine-rich) repeats.

Contains 1 TIR domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

flagQ560862EBI-6548397,EBI-6548623From a different organism.
fliCE1WGJ52EBI-6548397,EBI-6548383From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 859833Toll-like receptor 5
PRO_0000034730

Regions

Topological domain27 – 641615Extracellular Potential
Transmembrane642 – 66221Helical; Potential
Topological domain663 – 859197Cytoplasmic Potential
Repeat70 – 9425LRR 1
Repeat95 – 11824LRR 2
Repeat145 – 16925LRR 3
Repeat226 – 24924LRR 4
Repeat288 – 31124LRR 5
Repeat312 – 33524LRR 6
Repeat337 – 35923LRR 7
Repeat360 – 38324LRR 8
Repeat384 – 41027LRR 9
Repeat423 – 44624LRR 10
Repeat448 – 47124LRR 11
Repeat473 – 49725LRR 12
Repeat502 – 52524LRR 13
Repeat527 – 54923LRR 14
Repeat551 – 56919LRR 15
Repeat570 – 59324LRR 16
Domain692 – 838147TIR

Amino acid modifications

Modified residue7991Phosphotyrosine By similarity
Glycosylation371N-linked (GlcNAc...) Potential
Glycosylation461N-linked (GlcNAc...) Potential
Glycosylation841N-linked (GlcNAc...) Potential
Glycosylation2461N-linked (GlcNAc...) Potential
Glycosylation3431N-linked (GlcNAc...) Potential
Glycosylation4381N-linked (GlcNAc...) Potential
Glycosylation5961N-linked (GlcNAc...) Potential
Glycosylation5991N-linked (GlcNAc...) Potential
Glycosylation6241N-linked (GlcNAc...) Potential
Disulfide bond584 ↔ 611 By similarity
Disulfide bond586 ↔ 630 By similarity

Natural variations

Natural variant2261G → D in strain: MOLF/Ei.
Natural variant3991A → T in strain: MOLF/Ei.
Natural variant4701P → T in strain: MOLF/Ei.
Natural variant4821T → A in strain: MOLF/Ei.
Natural variant6091V → M in strain: MOLF/Ei.
Natural variant6191G → A in strain: MOLF/Ei.
Natural variant8351G → E in strain: MOLF/Ei.
Natural variant8431G → A in strain: MOLF/Ei.

Sequences

Sequence LengthMass (Da)Tools
Q9JLF7 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 74CD915EA3F0275F

FASTA85997,627
        10         20         30         40         50         60 
MACQLDLLIG VIFMASPVLV ISPCSSDGRI AFFRGCNLTQ IPWILNTTTE RLLLSFNYIS 

        70         80         90        100        110        120 
MVVATSFPLL ERLQLLELGT QYANLTIGPG AFRNLPNLRI LDLGQSQIEV LNRDAFQGLP 

       130        140        150        160        170        180 
HLLELRLFSC GLSSAVLSDG YFRNLYSLAR LDLSGNQIHS LRLHSSFREL NSLSDVNFAF 

       190        200        210        220        230        240 
NQIFTICEDE LEPLQGKTLS FFGLKLTKLF SRVSVGWETC RNPFRGVRLE TLDLSENGWT 

       250        260        270        280        290        300 
VDITRNFSNI IQGSQISSLI LKHHIMGPGF GFQNIRDPDQ STFASLARSS VLQLDLSHGF 

       310        320        330        340        350        360 
IFSLNPRLFG TLKDLKMLNL AFNKINKIGE NAFYGLDSLQ VLNLSYNLLG ELYNSNFYGL 

       370        380        390        400        410        420 
PRVAYVDLQR NHIGIIQDQT FRLLKTLQTL DLRDNALKAI GFIPSIQMVL LGGNKLVHLP 

       430        440        450        460        470        480 
HIHFTANFLE LSENRLENLS DLYFLLRVPQ LQFLILNQNR LSSCKAAHTP SENPSLEQLF 

       490        500        510        520        530        540 
LTENMLQLAW ETGLCWDVFQ GLSRLQILYL SNNYLNFLPP GIFNDLVALR MLSLSANKLT 

       550        560        570        580        590        600 
VLSPGSLPAN LEILDISRNQ LLCPDPALFS SLRVLDITHN EFVCNCELST FISWLNQTNV 

       610        620        630        640        650        660 
TLFGSPADVY CMYPNSLLGG SLYNISTEDC DEEEAMRSLK FSLFILCTVT LTLFLVITLV 

       670        680        690        700        710        720 
VIKFRGICFL CYKTIQKLVF KDKVWSLEPG AYRYDAYFCF SSKDFEWAQN ALLKHLDAHY 

       730        740        750        760        770        780 
SSRNRLRLCF EERDFIPGEN HISNIQAAVW GSRKTVCLVS RHFLKDGWCL EAFRYAQSRS 

       790        800        810        820        830        840 
LSDLKSILIV VVVGSLSQYQ LMRHETIRGF LQKQQYLRWP EDLQDVGWFL DKLSGCILKE 

       850 
EKGKKRSSSI QLRTIATIS 

« Hide

References

[1]"Cloning and characterization of the murine Toll-like receptor 5 (Tlr5) gene: sequence and mRNA expression studies in Salmonella-susceptible MOLF/Ei mice."
Sebastiani G., Leveque G., Lariviere L., Laroche L., Skamene E., Gros P., Malo D.
Genomics 64:230-240(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
Strain: C57BL/6J and MOLF/Ei.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF186107 mRNA. Translation: AAF65625.1.
RefSeqNP_058624.2. NM_016928.2.
UniGeneMm.116894.

3D structure databases

ProteinModelPortalQ9JLF7.
SMRQ9JLF7. Positions 24-838.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9JLF7. 3 interactions.

PTM databases

PhosphoSiteQ9JLF7.

Proteomic databases

PRIDEQ9JLF7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID53791.
KEGGmmu:53791.

Organism-specific databases

CTD7100.
MGIMGI:1858171. Tlr5.

Phylogenomic databases

eggNOGNOG271351.
HOGENOMHOG000008675.
HOVERGENHBG023182.
InParanoidQ9JLF7.
KOK10168.
PhylomeDBQ9JLF7.

Gene expression databases

CleanExMM_TLR5.
GenevestigatorQ9JLF7.

Family and domain databases

Gene3D3.40.50.10140. 1 hit.
InterProIPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000157. TIR_dom.
IPR027176. TLR5.
[Graphical view]
PANTHERPTHR24365:SF148. PTHR24365:SF148. 1 hit.
PfamPF01582. TIR. 1 hit.
[Graphical view]
SMARTSM00369. LRR_TYP. 2 hits.
SM00082. LRRCT. 1 hit.
SM00255. TIR. 1 hit.
[Graphical view]
SUPFAMSSF52200. SSF52200. 1 hit.
PROSITEPS51450. LRR. 14 hits.
PS50104. TIR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio310633.
PROQ9JLF7.
SOURCESearch...

Entry information

Entry nameTLR5_MOUSE
AccessionPrimary (citable) accession number: Q9JLF7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot