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Protein

Toll-like receptor 5

Gene

Tlr5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Toll-like receptor 5
Gene namesi
Name:Tlr5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1858171. Tlr5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini27 – 641ExtracellularSequence analysisAdd BLAST615
Transmembranei642 – 662HelicalSequence analysisAdd BLAST21
Topological domaini663 – 859CytoplasmicSequence analysisAdd BLAST197

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000003473027 – 859Toll-like receptor 5Add BLAST833

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi37N-linked (GlcNAc...)Sequence analysis1
Glycosylationi46N-linked (GlcNAc...)Sequence analysis1
Glycosylationi84N-linked (GlcNAc...)Sequence analysis1
Glycosylationi246N-linked (GlcNAc...)Sequence analysis1
Glycosylationi343N-linked (GlcNAc...)Sequence analysis1
Glycosylationi438N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi584 ↔ 611By similarity
Disulfide bondi586 ↔ 630By similarity
Glycosylationi596N-linked (GlcNAc...)Sequence analysis1
Glycosylationi599N-linked (GlcNAc...)Sequence analysis1
Glycosylationi624N-linked (GlcNAc...)Sequence analysis1
Modified residuei799PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated at Tyr-799 upon flagellin binding; required for signaling.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9JLF7.
PRIDEiQ9JLF7.

PTM databases

iPTMnetiQ9JLF7.
PhosphoSitePlusiQ9JLF7.

Expressioni

Tissue specificityi

Highly expressed in liver. Detected in lung and at very low levels in most other tissues.

Gene expression databases

CleanExiMM_TLR5.

Interactioni

Subunit structurei

Homodimer both in the absence and presence of ligand. Binds MYD88 via their respective TIR domains (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
flagQ560862EBI-6548397,EBI-6548623From a different organism.
fliCA0A0H3NMJ62EBI-6548397,EBI-6548383From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9JLF7. 3 interactors.
STRINGi10090.ENSMUSP00000106625.

Structurei

3D structure databases

ProteinModelPortaliQ9JLF7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati45 – 69LRR 1Add BLAST25
Repeati72 – 94LRR 2Add BLAST23
Repeati96 – 118LRR 3Add BLAST23
Repeati121 – 144LRR 4Add BLAST24
Repeati147 – 167LRR 5Add BLAST21
Repeati172 – 193LRR 6Add BLAST22
Repeati198 – 212LRR 7Add BLAST15
Repeati215 – 230LRR 8Add BLAST16
Repeati235 – 236LRR 92
Repeati261 – 285LRR 11Add BLAST25
Repeati290 – 302LRR 12Add BLAST13
Repeati314 – 335LRR 13Add BLAST22
Repeati338 – 356LRR 14Add BLAST19
Repeati386 – 402LRR 16Add BLAST17
Repeati413 – 432LRR 17Add BLAST20
Repeati450 – 471LRR 18Add BLAST22
Repeati475 – 496LRR 19Add BLAST22
Repeati504 – 525LRR 20Add BLAST22
Repeati528 – 547LRR 21Add BLAST20
Repeati550 – 568LRR 22Add BLAST19
Domaini580 – 632LRRCTAdd BLAST53
Domaini692 – 838TIRPROSITE-ProRule annotationAdd BLAST147

Sequence similaritiesi

Belongs to the Toll-like receptor family.Curated
Contains 22 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 TIR domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4641. Eukaryota.
COG4886. LUCA.
HOGENOMiHOG000008675.
HOVERGENiHBG023182.
InParanoidiQ9JLF7.
KOiK10168.
PhylomeDBiQ9JLF7.

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
3.80.10.10. 3 hits.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000157. TIR_dom.
IPR027176. TLR5.
[Graphical view]
PANTHERiPTHR24365:SF311. PTHR24365:SF311. 1 hit.
PfamiPF13855. LRR_8. 3 hits.
PF01582. TIR. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 9 hits.
SM00082. LRRCT. 1 hit.
SM00255. TIR. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 2 hits.
SSF52200. SSF52200. 1 hit.
PROSITEiPS51450. LRR. 14 hits.
PS50104. TIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JLF7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACQLDLLIG VIFMASPVLV ISPCSSDGRI AFFRGCNLTQ IPWILNTTTE
60 70 80 90 100
RLLLSFNYIS MVVATSFPLL ERLQLLELGT QYANLTIGPG AFRNLPNLRI
110 120 130 140 150
LDLGQSQIEV LNRDAFQGLP HLLELRLFSC GLSSAVLSDG YFRNLYSLAR
160 170 180 190 200
LDLSGNQIHS LRLHSSFREL NSLSDVNFAF NQIFTICEDE LEPLQGKTLS
210 220 230 240 250
FFGLKLTKLF SRVSVGWETC RNPFRGVRLE TLDLSENGWT VDITRNFSNI
260 270 280 290 300
IQGSQISSLI LKHHIMGPGF GFQNIRDPDQ STFASLARSS VLQLDLSHGF
310 320 330 340 350
IFSLNPRLFG TLKDLKMLNL AFNKINKIGE NAFYGLDSLQ VLNLSYNLLG
360 370 380 390 400
ELYNSNFYGL PRVAYVDLQR NHIGIIQDQT FRLLKTLQTL DLRDNALKAI
410 420 430 440 450
GFIPSIQMVL LGGNKLVHLP HIHFTANFLE LSENRLENLS DLYFLLRVPQ
460 470 480 490 500
LQFLILNQNR LSSCKAAHTP SENPSLEQLF LTENMLQLAW ETGLCWDVFQ
510 520 530 540 550
GLSRLQILYL SNNYLNFLPP GIFNDLVALR MLSLSANKLT VLSPGSLPAN
560 570 580 590 600
LEILDISRNQ LLCPDPALFS SLRVLDITHN EFVCNCELST FISWLNQTNV
610 620 630 640 650
TLFGSPADVY CMYPNSLLGG SLYNISTEDC DEEEAMRSLK FSLFILCTVT
660 670 680 690 700
LTLFLVITLV VIKFRGICFL CYKTIQKLVF KDKVWSLEPG AYRYDAYFCF
710 720 730 740 750
SSKDFEWAQN ALLKHLDAHY SSRNRLRLCF EERDFIPGEN HISNIQAAVW
760 770 780 790 800
GSRKTVCLVS RHFLKDGWCL EAFRYAQSRS LSDLKSILIV VVVGSLSQYQ
810 820 830 840 850
LMRHETIRGF LQKQQYLRWP EDLQDVGWFL DKLSGCILKE EKGKKRSSSI

QLRTIATIS
Length:859
Mass (Da):97,627
Last modified:October 1, 2000 - v1
Checksum:i74CD915EA3F0275F
GO

Polymorphismi

The TLR5 gene lies in a locus that is associated with susceptibility to Salmonella. Inbred strains of mice can be classified into 3 categories according to their resistance to infection with S.typhimurium: susceptible (BALB/c, C57BL/6, C3H/He), intermediate (DBA/2, C75L) and resistant (A, CBA). The strain MOLF/Ei is highly susceptible to the infection, has an unique TLR5 haplotype and a lower expression of TRL5.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti226G → D in strain: MOLF/Ei. 1
Natural varianti399A → T in strain: MOLF/Ei. 1
Natural varianti470P → T in strain: MOLF/Ei. 1
Natural varianti482T → A in strain: MOLF/Ei. 1
Natural varianti609V → M in strain: MOLF/Ei. 1
Natural varianti619G → A in strain: MOLF/Ei. 1
Natural varianti835G → E in strain: MOLF/Ei. 1
Natural varianti843G → A in strain: MOLF/Ei. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF186107 mRNA. Translation: AAF65625.1.
RefSeqiNP_058624.2. NM_016928.3.
UniGeneiMm.116894.

Genome annotation databases

GeneIDi53791.
KEGGimmu:53791.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF186107 mRNA. Translation: AAF65625.1.
RefSeqiNP_058624.2. NM_016928.3.
UniGeneiMm.116894.

3D structure databases

ProteinModelPortaliQ9JLF7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JLF7. 3 interactors.
STRINGi10090.ENSMUSP00000106625.

PTM databases

iPTMnetiQ9JLF7.
PhosphoSitePlusiQ9JLF7.

Proteomic databases

PaxDbiQ9JLF7.
PRIDEiQ9JLF7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi53791.
KEGGimmu:53791.

Organism-specific databases

CTDi7100.
MGIiMGI:1858171. Tlr5.

Phylogenomic databases

eggNOGiKOG4641. Eukaryota.
COG4886. LUCA.
HOGENOMiHOG000008675.
HOVERGENiHBG023182.
InParanoidiQ9JLF7.
KOiK10168.
PhylomeDBiQ9JLF7.

Miscellaneous databases

PROiQ9JLF7.
SOURCEiSearch...

Gene expression databases

CleanExiMM_TLR5.

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
3.80.10.10. 3 hits.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000157. TIR_dom.
IPR027176. TLR5.
[Graphical view]
PANTHERiPTHR24365:SF311. PTHR24365:SF311. 1 hit.
PfamiPF13855. LRR_8. 3 hits.
PF01582. TIR. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 9 hits.
SM00082. LRRCT. 1 hit.
SM00255. TIR. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 2 hits.
SSF52200. SSF52200. 1 hit.
PROSITEiPS51450. LRR. 14 hits.
PS50104. TIR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTLR5_MOUSE
AccessioniPrimary (citable) accession number: Q9JLF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.