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Q9JLF7

- TLR5_MOUSE

UniProt

Q9JLF7 - TLR5_MOUSE

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Protein

Toll-like receptor 5

Gene

Tlr5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity).By similarity

GO - Molecular functioni

  1. transmembrane signaling receptor activity Source: InterPro

GO - Biological processi

  1. defense response to bacterium Source: InterPro
  2. inflammatory response Source: UniProtKB-KW
  3. innate immune response Source: UniProtKB-KW
  4. MyD88-dependent toll-like receptor signaling pathway Source: InterPro
  5. positive regulation of cytokine production Source: InterPro
  6. regulation of cytokine secretion Source: InterPro
  7. toll-like receptor 5 signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Toll-like receptor 5
Gene namesi
Name:Tlr5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1858171. Tlr5.

Subcellular locationi

Membrane By similarity; Single-pass type I membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 641615ExtracellularSequence AnalysisAdd
BLAST
Transmembranei642 – 66221HelicalSequence AnalysisAdd
BLAST
Topological domaini663 – 859197CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 859833Toll-like receptor 5PRO_0000034730Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi37 – 371N-linked (GlcNAc...)Sequence Analysis
Glycosylationi46 – 461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi343 – 3431N-linked (GlcNAc...)Sequence Analysis
Glycosylationi438 – 4381N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi584 ↔ 611By similarity
Disulfide bondi586 ↔ 630By similarity
Glycosylationi596 – 5961N-linked (GlcNAc...)Sequence Analysis
Glycosylationi599 – 5991N-linked (GlcNAc...)Sequence Analysis
Glycosylationi624 – 6241N-linked (GlcNAc...)Sequence Analysis
Modified residuei799 – 7991PhosphotyrosineBy similarity

Post-translational modificationi

Phosphorylated at Tyr-799 upon flagellin binding; required for signaling.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ9JLF7.

PTM databases

PhosphoSiteiQ9JLF7.

Expressioni

Tissue specificityi

Highly expressed in liver. Detected in lung and at very low levels in most other tissues.

Gene expression databases

CleanExiMM_TLR5.
GenevestigatoriQ9JLF7.

Interactioni

Subunit structurei

Homodimer both in the absence and presence of ligand. Binds MYD88 via their respective TIR domains (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
flagQ560862EBI-6548397,EBI-6548623From a different organism.
fliCE1WGJ52EBI-6548397,EBI-6548383From a different organism.

Protein-protein interaction databases

IntActiQ9JLF7. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9JLF7.
SMRiQ9JLF7. Positions 24-838.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati45 – 6925LRR 1Add
BLAST
Repeati72 – 9423LRR 2Add
BLAST
Repeati96 – 11823LRR 3Add
BLAST
Repeati121 – 14424LRR 4Add
BLAST
Repeati147 – 16721LRR 5Add
BLAST
Repeati172 – 19322LRR 6Add
BLAST
Repeati198 – 21215LRR 7Add
BLAST
Repeati215 – 23016LRR 8Add
BLAST
Repeati235 – 2362LRR 9
Repeati261 – 28525LRR 11Add
BLAST
Repeati290 – 30213LRR 12Add
BLAST
Repeati314 – 33522LRR 13Add
BLAST
Repeati338 – 35619LRR 14Add
BLAST
Repeati386 – 40217LRR 16Add
BLAST
Repeati413 – 43220LRR 17Add
BLAST
Repeati450 – 47122LRR 18Add
BLAST
Repeati475 – 49622LRR 19Add
BLAST
Repeati504 – 52522LRR 20Add
BLAST
Repeati528 – 54720LRR 21Add
BLAST
Repeati550 – 56819LRR 22Add
BLAST
Domaini580 – 63253LRRCTAdd
BLAST
Domaini692 – 838147TIRPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the Toll-like receptor family.Curated
Contains 22 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 TIR domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG271351.
HOGENOMiHOG000008675.
HOVERGENiHBG023182.
InParanoidiQ9JLF7.
KOiK10168.
PhylomeDBiQ9JLF7.

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000157. TIR_dom.
IPR027176. TLR5.
[Graphical view]
PANTHERiPTHR24365:SF221. PTHR24365:SF221. 1 hit.
PfamiPF13504. LRR_7. 1 hit.
PF13855. LRR_8. 3 hits.
PF01582. TIR. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 2 hits.
SM00082. LRRCT. 1 hit.
SM00255. TIR. 1 hit.
[Graphical view]
SUPFAMiSSF52200. SSF52200. 1 hit.
PROSITEiPS51450. LRR. 14 hits.
PS50104. TIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JLF7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MACQLDLLIG VIFMASPVLV ISPCSSDGRI AFFRGCNLTQ IPWILNTTTE
60 70 80 90 100
RLLLSFNYIS MVVATSFPLL ERLQLLELGT QYANLTIGPG AFRNLPNLRI
110 120 130 140 150
LDLGQSQIEV LNRDAFQGLP HLLELRLFSC GLSSAVLSDG YFRNLYSLAR
160 170 180 190 200
LDLSGNQIHS LRLHSSFREL NSLSDVNFAF NQIFTICEDE LEPLQGKTLS
210 220 230 240 250
FFGLKLTKLF SRVSVGWETC RNPFRGVRLE TLDLSENGWT VDITRNFSNI
260 270 280 290 300
IQGSQISSLI LKHHIMGPGF GFQNIRDPDQ STFASLARSS VLQLDLSHGF
310 320 330 340 350
IFSLNPRLFG TLKDLKMLNL AFNKINKIGE NAFYGLDSLQ VLNLSYNLLG
360 370 380 390 400
ELYNSNFYGL PRVAYVDLQR NHIGIIQDQT FRLLKTLQTL DLRDNALKAI
410 420 430 440 450
GFIPSIQMVL LGGNKLVHLP HIHFTANFLE LSENRLENLS DLYFLLRVPQ
460 470 480 490 500
LQFLILNQNR LSSCKAAHTP SENPSLEQLF LTENMLQLAW ETGLCWDVFQ
510 520 530 540 550
GLSRLQILYL SNNYLNFLPP GIFNDLVALR MLSLSANKLT VLSPGSLPAN
560 570 580 590 600
LEILDISRNQ LLCPDPALFS SLRVLDITHN EFVCNCELST FISWLNQTNV
610 620 630 640 650
TLFGSPADVY CMYPNSLLGG SLYNISTEDC DEEEAMRSLK FSLFILCTVT
660 670 680 690 700
LTLFLVITLV VIKFRGICFL CYKTIQKLVF KDKVWSLEPG AYRYDAYFCF
710 720 730 740 750
SSKDFEWAQN ALLKHLDAHY SSRNRLRLCF EERDFIPGEN HISNIQAAVW
760 770 780 790 800
GSRKTVCLVS RHFLKDGWCL EAFRYAQSRS LSDLKSILIV VVVGSLSQYQ
810 820 830 840 850
LMRHETIRGF LQKQQYLRWP EDLQDVGWFL DKLSGCILKE EKGKKRSSSI

QLRTIATIS
Length:859
Mass (Da):97,627
Last modified:October 1, 2000 - v1
Checksum:i74CD915EA3F0275F
GO

Polymorphismi

The TLR5 gene lies in a locus that is associated with susceptibility to Salmonella. Inbred strains of mice can be classified into 3 categories according to their resistance to infection with S.typhimurium: susceptible (BALB/c, C57BL/6, C3H/He), intermediate (DBA/2, C75L) and resistant (A, CBA). The strain MOLF/Ei is highly susceptible to the infection, has an unique TLR5 haplotype and a lower expression of TRL5.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti226 – 2261G → D in strain: MOLF/Ei.
Natural varianti399 – 3991A → T in strain: MOLF/Ei.
Natural varianti470 – 4701P → T in strain: MOLF/Ei.
Natural varianti482 – 4821T → A in strain: MOLF/Ei.
Natural varianti609 – 6091V → M in strain: MOLF/Ei.
Natural varianti619 – 6191G → A in strain: MOLF/Ei.
Natural varianti835 – 8351G → E in strain: MOLF/Ei.
Natural varianti843 – 8431G → A in strain: MOLF/Ei.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF186107 mRNA. Translation: AAF65625.1.
RefSeqiNP_058624.2. NM_016928.2.
UniGeneiMm.116894.

Genome annotation databases

GeneIDi53791.
KEGGimmu:53791.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF186107 mRNA. Translation: AAF65625.1 .
RefSeqi NP_058624.2. NM_016928.2.
UniGenei Mm.116894.

3D structure databases

ProteinModelPortali Q9JLF7.
SMRi Q9JLF7. Positions 24-838.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9JLF7. 3 interactions.

PTM databases

PhosphoSitei Q9JLF7.

Proteomic databases

PRIDEi Q9JLF7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 53791.
KEGGi mmu:53791.

Organism-specific databases

CTDi 7100.
MGIi MGI:1858171. Tlr5.

Phylogenomic databases

eggNOGi NOG271351.
HOGENOMi HOG000008675.
HOVERGENi HBG023182.
InParanoidi Q9JLF7.
KOi K10168.
PhylomeDBi Q9JLF7.

Miscellaneous databases

NextBioi 310633.
PROi Q9JLF7.
SOURCEi Search...

Gene expression databases

CleanExi MM_TLR5.
Genevestigatori Q9JLF7.

Family and domain databases

Gene3Di 3.40.50.10140. 1 hit.
InterProi IPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000157. TIR_dom.
IPR027176. TLR5.
[Graphical view ]
PANTHERi PTHR24365:SF221. PTHR24365:SF221. 1 hit.
Pfami PF13504. LRR_7. 1 hit.
PF13855. LRR_8. 3 hits.
PF01582. TIR. 1 hit.
[Graphical view ]
SMARTi SM00369. LRR_TYP. 2 hits.
SM00082. LRRCT. 1 hit.
SM00255. TIR. 1 hit.
[Graphical view ]
SUPFAMi SSF52200. SSF52200. 1 hit.
PROSITEi PS51450. LRR. 14 hits.
PS50104. TIR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the murine Toll-like receptor 5 (Tlr5) gene: sequence and mRNA expression studies in Salmonella-susceptible MOLF/Ei mice."
    Sebastiani G., Leveque G., Lariviere L., Laroche L., Skamene E., Gros P., Malo D.
    Genomics 64:230-240(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
    Strain: C57BL/6J and MOLF/Ei.
    Tissue: Liver.

Entry informationi

Entry nameiTLR5_MOUSE
AccessioniPrimary (citable) accession number: Q9JLF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3