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Q9JLF7

- TLR5_MOUSE

UniProt

Q9JLF7 - TLR5_MOUSE

Protein

Toll-like receptor 5

Gene

Tlr5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. transmembrane signaling receptor activity Source: InterPro

    GO - Biological processi

    1. defense response to bacterium Source: InterPro
    2. inflammatory response Source: UniProtKB-KW
    3. innate immune response Source: UniProtKB-KW
    4. MyD88-dependent toll-like receptor signaling pathway Source: InterPro
    5. positive regulation of cytokine production Source: InterPro
    6. regulation of cytokine secretion Source: InterPro
    7. toll-like receptor 5 signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Immunity, Inflammatory response, Innate immunity

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Toll-like receptor 5
    Gene namesi
    Name:Tlr5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1858171. Tlr5.

    Subcellular locationi

    Membrane By similarity; Single-pass type I membrane protein By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 859833Toll-like receptor 5PRO_0000034730Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi37 – 371N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi46 – 461N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi343 – 3431N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi438 – 4381N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi584 ↔ 611By similarity
    Disulfide bondi586 ↔ 630By similarity
    Glycosylationi596 – 5961N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi599 – 5991N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi624 – 6241N-linked (GlcNAc...)Sequence Analysis
    Modified residuei799 – 7991PhosphotyrosineBy similarity

    Post-translational modificationi

    Phosphorylated at Tyr-799 upon flagellin binding; required for signaling.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiQ9JLF7.

    PTM databases

    PhosphoSiteiQ9JLF7.

    Expressioni

    Tissue specificityi

    Highly expressed in liver. Detected in lung and at very low levels in most other tissues.

    Gene expression databases

    CleanExiMM_TLR5.
    GenevestigatoriQ9JLF7.

    Interactioni

    Subunit structurei

    Homodimer both in the absence and presence of ligand. Binds MYD88 via their respective TIR domains By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    flagQ560862EBI-6548397,EBI-6548623From a different organism.
    fliCE1WGJ52EBI-6548397,EBI-6548383From a different organism.

    Protein-protein interaction databases

    IntActiQ9JLF7. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JLF7.
    SMRiQ9JLF7. Positions 24-838.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini27 – 641615ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini663 – 859197CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei642 – 66221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati45 – 6925LRR 1Add
    BLAST
    Repeati72 – 9423LRR 2Add
    BLAST
    Repeati96 – 11823LRR 3Add
    BLAST
    Repeati121 – 14424LRR 4Add
    BLAST
    Repeati147 – 16721LRR 5Add
    BLAST
    Repeati172 – 19322LRR 6Add
    BLAST
    Repeati198 – 21215LRR 7Add
    BLAST
    Repeati215 – 23016LRR 8Add
    BLAST
    Repeati235 – 2362LRR 9
    Repeati261 – 28525LRR 11Add
    BLAST
    Repeati290 – 30213LRR 12Add
    BLAST
    Repeati314 – 33522LRR 13Add
    BLAST
    Repeati338 – 35619LRR 14Add
    BLAST
    Repeati386 – 40217LRR 16Add
    BLAST
    Repeati413 – 43220LRR 17Add
    BLAST
    Repeati450 – 47122LRR 18Add
    BLAST
    Repeati475 – 49622LRR 19Add
    BLAST
    Repeati504 – 52522LRR 20Add
    BLAST
    Repeati528 – 54720LRR 21Add
    BLAST
    Repeati550 – 56819LRR 22Add
    BLAST
    Domaini580 – 63253LRRCTAdd
    BLAST
    Domaini692 – 838147TIRPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Toll-like receptor family.Curated
    Contains 22 LRR (leucine-rich) repeats.Curated
    Contains 1 LRRCT domain.Curated
    Contains 1 TIR domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG271351.
    HOGENOMiHOG000008675.
    HOVERGENiHBG023182.
    InParanoidiQ9JLF7.
    KOiK10168.
    PhylomeDBiQ9JLF7.

    Family and domain databases

    Gene3Di3.40.50.10140. 1 hit.
    InterProiIPR000483. Cys-rich_flank_reg_C.
    IPR001611. Leu-rich_rpt.
    IPR003591. Leu-rich_rpt_typical-subtyp.
    IPR000157. TIR_dom.
    IPR027176. TLR5.
    [Graphical view]
    PANTHERiPTHR24365:SF221. PTHR24365:SF221. 1 hit.
    PfamiPF13504. LRR_7. 1 hit.
    PF13855. LRR_8. 3 hits.
    PF01582. TIR. 1 hit.
    [Graphical view]
    SMARTiSM00369. LRR_TYP. 2 hits.
    SM00082. LRRCT. 1 hit.
    SM00255. TIR. 1 hit.
    [Graphical view]
    SUPFAMiSSF52200. SSF52200. 1 hit.
    PROSITEiPS51450. LRR. 14 hits.
    PS50104. TIR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9JLF7-1 [UniParc]FASTAAdd to Basket

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    MACQLDLLIG VIFMASPVLV ISPCSSDGRI AFFRGCNLTQ IPWILNTTTE    50
    RLLLSFNYIS MVVATSFPLL ERLQLLELGT QYANLTIGPG AFRNLPNLRI 100
    LDLGQSQIEV LNRDAFQGLP HLLELRLFSC GLSSAVLSDG YFRNLYSLAR 150
    LDLSGNQIHS LRLHSSFREL NSLSDVNFAF NQIFTICEDE LEPLQGKTLS 200
    FFGLKLTKLF SRVSVGWETC RNPFRGVRLE TLDLSENGWT VDITRNFSNI 250
    IQGSQISSLI LKHHIMGPGF GFQNIRDPDQ STFASLARSS VLQLDLSHGF 300
    IFSLNPRLFG TLKDLKMLNL AFNKINKIGE NAFYGLDSLQ VLNLSYNLLG 350
    ELYNSNFYGL PRVAYVDLQR NHIGIIQDQT FRLLKTLQTL DLRDNALKAI 400
    GFIPSIQMVL LGGNKLVHLP HIHFTANFLE LSENRLENLS DLYFLLRVPQ 450
    LQFLILNQNR LSSCKAAHTP SENPSLEQLF LTENMLQLAW ETGLCWDVFQ 500
    GLSRLQILYL SNNYLNFLPP GIFNDLVALR MLSLSANKLT VLSPGSLPAN 550
    LEILDISRNQ LLCPDPALFS SLRVLDITHN EFVCNCELST FISWLNQTNV 600
    TLFGSPADVY CMYPNSLLGG SLYNISTEDC DEEEAMRSLK FSLFILCTVT 650
    LTLFLVITLV VIKFRGICFL CYKTIQKLVF KDKVWSLEPG AYRYDAYFCF 700
    SSKDFEWAQN ALLKHLDAHY SSRNRLRLCF EERDFIPGEN HISNIQAAVW 750
    GSRKTVCLVS RHFLKDGWCL EAFRYAQSRS LSDLKSILIV VVVGSLSQYQ 800
    LMRHETIRGF LQKQQYLRWP EDLQDVGWFL DKLSGCILKE EKGKKRSSSI 850
    QLRTIATIS 859
    Length:859
    Mass (Da):97,627
    Last modified:October 1, 2000 - v1
    Checksum:i74CD915EA3F0275F
    GO

    Polymorphismi

    The TLR5 gene lies in a locus that is associated with susceptibility to Salmonella. Inbred strains of mice can be classified into 3 categories according to their resistance to infection with S.typhimurium: susceptible (BALB/c, C57BL/6, C3H/He), intermediate (DBA/2, C75L) and resistant (A, CBA). The strain MOLF/Ei is highly susceptible to the infection, has an unique TLR5 haplotype and a lower expression of TRL5.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti226 – 2261G → D in strain: MOLF/Ei.
    Natural varianti399 – 3991A → T in strain: MOLF/Ei.
    Natural varianti470 – 4701P → T in strain: MOLF/Ei.
    Natural varianti482 – 4821T → A in strain: MOLF/Ei.
    Natural varianti609 – 6091V → M in strain: MOLF/Ei.
    Natural varianti619 – 6191G → A in strain: MOLF/Ei.
    Natural varianti835 – 8351G → E in strain: MOLF/Ei.
    Natural varianti843 – 8431G → A in strain: MOLF/Ei.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF186107 mRNA. Translation: AAF65625.1.
    RefSeqiNP_058624.2. NM_016928.2.
    UniGeneiMm.116894.

    Genome annotation databases

    GeneIDi53791.
    KEGGimmu:53791.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF186107 mRNA. Translation: AAF65625.1 .
    RefSeqi NP_058624.2. NM_016928.2.
    UniGenei Mm.116894.

    3D structure databases

    ProteinModelPortali Q9JLF7.
    SMRi Q9JLF7. Positions 24-838.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9JLF7. 3 interactions.

    PTM databases

    PhosphoSitei Q9JLF7.

    Proteomic databases

    PRIDEi Q9JLF7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 53791.
    KEGGi mmu:53791.

    Organism-specific databases

    CTDi 7100.
    MGIi MGI:1858171. Tlr5.

    Phylogenomic databases

    eggNOGi NOG271351.
    HOGENOMi HOG000008675.
    HOVERGENi HBG023182.
    InParanoidi Q9JLF7.
    KOi K10168.
    PhylomeDBi Q9JLF7.

    Miscellaneous databases

    NextBioi 310633.
    PROi Q9JLF7.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_TLR5.
    Genevestigatori Q9JLF7.

    Family and domain databases

    Gene3Di 3.40.50.10140. 1 hit.
    InterProi IPR000483. Cys-rich_flank_reg_C.
    IPR001611. Leu-rich_rpt.
    IPR003591. Leu-rich_rpt_typical-subtyp.
    IPR000157. TIR_dom.
    IPR027176. TLR5.
    [Graphical view ]
    PANTHERi PTHR24365:SF221. PTHR24365:SF221. 1 hit.
    Pfami PF13504. LRR_7. 1 hit.
    PF13855. LRR_8. 3 hits.
    PF01582. TIR. 1 hit.
    [Graphical view ]
    SMARTi SM00369. LRR_TYP. 2 hits.
    SM00082. LRRCT. 1 hit.
    SM00255. TIR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52200. SSF52200. 1 hit.
    PROSITEi PS51450. LRR. 14 hits.
    PS50104. TIR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the murine Toll-like receptor 5 (Tlr5) gene: sequence and mRNA expression studies in Salmonella-susceptible MOLF/Ei mice."
      Sebastiani G., Leveque G., Lariviere L., Laroche L., Skamene E., Gros P., Malo D.
      Genomics 64:230-240(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
      Strain: C57BL/6J and MOLF/Ei.
      Tissue: Liver.

    Entry informationi

    Entry nameiTLR5_MOUSE
    AccessioniPrimary (citable) accession number: Q9JLF7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 31, 2002
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3