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Q9JLF6

- TGM1_MOUSE

UniProt

Q9JLF6 - TGM1_MOUSE

Protein

Protein-glutamine gamma-glutamyltransferase K

Gene

Tgm1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (04 Jan 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Responsible for cross-linking epidermal proteins during formation of the stratum corneum By similarity.By similarity

    Catalytic activityi

    Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.PROSITE-ProRule annotation

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei376 – 3761PROSITE-ProRule annotation
    Active sitei435 – 4351PROSITE-ProRule annotation
    Active sitei458 – 4581PROSITE-ProRule annotation
    Metal bindingi498 – 4981CalciumBy similarity
    Metal bindingi500 – 5001CalciumBy similarity
    Metal bindingi547 – 5471CalciumBy similarity
    Metal bindingi552 – 5521CalciumBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein-glutamine gamma-glutamyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. keratinization Source: UniProtKB-KW
    2. organ morphogenesis Source: MGI
    3. peptide cross-linking Source: InterPro
    4. protein metabolic process Source: MGI

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Keratinization

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein-glutamine gamma-glutamyltransferase K (EC:2.3.2.13)
    Alternative name(s):
    Epidermal TGase
    Transglutaminase K
    Short name:
    TG(K)
    Short name:
    TGK
    Short name:
    TGase K
    Transglutaminase-1
    Short name:
    TGase-1
    Gene namesi
    Name:Tgm1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:98730. Tgm1.

    Subcellular locationi

    Membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. cell-cell adherens junction Source: MGI
    2. intrinsic component of membrane Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 815815Protein-glutamine gamma-glutamyltransferase KPRO_0000213702Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei23 – 231PhosphoserineBy similarity
    Modified residuei83 – 831PhosphoserineBy similarity
    Modified residuei91 – 911Phosphoserine2 Publications
    Modified residuei94 – 941Phosphoserine3 Publications
    Modified residuei803 – 8031Phosphoserine2 Publications

    Post-translational modificationi

    Tyrosine-phosphorylated.3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9JLF6.
    PaxDbiQ9JLF6.
    PRIDEiQ9JLF6.

    PTM databases

    PhosphoSiteiQ9JLF6.

    Expressioni

    Tissue specificityi

    Expressed in large amounts in epithelial tissues (lung, liver and kidney).1 Publication

    Gene expression databases

    BgeeiQ9JLF6.
    CleanExiMM_TGM1.
    GenevestigatoriQ9JLF6.

    Interactioni

    Protein-protein interaction databases

    IntActiQ9JLF6. 3 interactions.
    MINTiMINT-4137626.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JLF6.
    SMRiQ9JLF6. Positions 85-786.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG80379.
    GeneTreeiENSGT00740000115156.
    HOGENOMiHOG000231695.
    HOVERGENiHBG004342.
    InParanoidiQ9JLF6.
    KOiK05619.
    OrthoDBiEOG7WT40M.
    PhylomeDBiQ9JLF6.
    TreeFamiTF324278.

    Family and domain databases

    Gene3Di2.60.40.10. 3 hits.
    3.90.260.10. 1 hit.
    InterProiIPR023608. Gln_gamma-glutamylTfrase_euk.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR002931. Transglutaminase-like.
    IPR008958. Transglutaminase_C.
    IPR013808. Transglutaminase_CS.
    IPR001102. Transglutaminase_N.
    [Graphical view]
    PANTHERiPTHR11590. PTHR11590. 1 hit.
    PfamiPF00927. Transglut_C. 2 hits.
    PF01841. Transglut_core. 1 hit.
    PF00868. Transglut_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000459. TGM_EBP42. 1 hit.
    SMARTiSM00460. TGc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49309. SSF49309. 2 hits.
    SSF81296. SSF81296. 1 hit.
    PROSITEiPS00547. TRANSGLUTAMINASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9JLF6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGPRSDVGR WGRSPWQPPT TPSPEPEPEP EPDRRSRSRR GGGRSFWARC    50
    CGCCSCGNRG DDDWGPEPSG SRSRGTSSRG RDSRGGRRPE SRGSGVNAAG 100
    DGTIREGMLV VTGVDLLCSR SDQNRREHHT DEFEYDELIV RRGQPFHMIL 150
    FLNREYESSD RIALELLIGS NPEVGKGTHV IIPVGKGGSG GWKAQVTKNN 200
    GHNLNLRVHT SPNAIIGKFQ FTVRTRSEAG EFQLPFDPRN EIYILFNPWC 250
    PEDIVYVDHE DWRQEYVLNE SGRIYYGTEA QIGERTWNYG QFDHGVLDAC 300
    LYILDRRGMP YGGRGDPVSV SRVVSAMVNS LDDNGVLIGN WTGDYSRGTN 350
    PSAWVGSVEI LLSYLRTGYS VPYGQCWVFA GVTTTVLRCL GFATRTVTNF 400
    NSAHDTDTSL TMDIYFDENM KPLEHLNHDS VWNFHVWNDC WMKRPDLPSG 450
    FDGWQVVDAT PQETSSGIFC CGPCSVESVK NGLVYMKYDT PFIFAEVNSD 500
    KVYWQRQDDG SFKIVYVEEK AIGTLIVTKA IHSNNREDIT HIYKHPEGSE 550
    AERRAVEKAA AHGSKPNVYA TRDSAEDVAM QVEAQDAVMG QDLAVSVVLT 600
    NRGSSRRTVK LHLYLCVTYY TGVSGPTFKE AKKEVTLAPG ASDSVTMPVA 650
    YKEYKPHLVD QGAMLLNVSG HVKESGQVLA KQHTFRLRTP DLSLTLLGAA 700
    VVGQECGVQI VFKNPLPVTL TNVVFRLEGS GLQRPKVLNV GDIGGNETVT 750
    LRQTFVPVRP GPRQLIASLD SPQLSQVHGV IQVDVAPASG GSGFSDAGGD 800
    SRSGENIPMA YRGGA 815
    Length:815
    Mass (Da):89,826
    Last modified:January 4, 2005 - v2
    Checksum:i326DD4E512AEA23E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti29 – 291E → V in AAF35986. (PubMed:10567386)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF186373 mRNA. Translation: AAF35986.1.
    BC026422 mRNA. Translation: AAH26422.1.
    CCDSiCCDS36932.1.
    RefSeqiNP_001155186.1. NM_001161714.1.
    NP_001155187.1. NM_001161715.1.
    NP_064368.3. NM_019984.3.
    XP_006518871.1. XM_006518808.1.
    XP_006518872.1. XM_006518809.1.
    UniGeneiMm.41964.

    Genome annotation databases

    EnsembliENSMUST00000002389; ENSMUSP00000002389; ENSMUSG00000022218.
    ENSMUST00000168729; ENSMUSP00000128090; ENSMUSG00000022218.
    ENSMUST00000178034; ENSMUSP00000137642; ENSMUSG00000022218.
    GeneIDi21816.
    KEGGimmu:21816.
    UCSCiuc007uag.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF186373 mRNA. Translation: AAF35986.1 .
    BC026422 mRNA. Translation: AAH26422.1 .
    CCDSi CCDS36932.1.
    RefSeqi NP_001155186.1. NM_001161714.1.
    NP_001155187.1. NM_001161715.1.
    NP_064368.3. NM_019984.3.
    XP_006518871.1. XM_006518808.1.
    XP_006518872.1. XM_006518809.1.
    UniGenei Mm.41964.

    3D structure databases

    ProteinModelPortali Q9JLF6.
    SMRi Q9JLF6. Positions 85-786.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9JLF6. 3 interactions.
    MINTi MINT-4137626.

    PTM databases

    PhosphoSitei Q9JLF6.

    Proteomic databases

    MaxQBi Q9JLF6.
    PaxDbi Q9JLF6.
    PRIDEi Q9JLF6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000002389 ; ENSMUSP00000002389 ; ENSMUSG00000022218 .
    ENSMUST00000168729 ; ENSMUSP00000128090 ; ENSMUSG00000022218 .
    ENSMUST00000178034 ; ENSMUSP00000137642 ; ENSMUSG00000022218 .
    GeneIDi 21816.
    KEGGi mmu:21816.
    UCSCi uc007uag.2. mouse.

    Organism-specific databases

    CTDi 7051.
    MGIi MGI:98730. Tgm1.

    Phylogenomic databases

    eggNOGi NOG80379.
    GeneTreei ENSGT00740000115156.
    HOGENOMi HOG000231695.
    HOVERGENi HBG004342.
    InParanoidi Q9JLF6.
    KOi K05619.
    OrthoDBi EOG7WT40M.
    PhylomeDBi Q9JLF6.
    TreeFami TF324278.

    Miscellaneous databases

    NextBioi 301220.
    PROi Q9JLF6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9JLF6.
    CleanExi MM_TGM1.
    Genevestigatori Q9JLF6.

    Family and domain databases

    Gene3Di 2.60.40.10. 3 hits.
    3.90.260.10. 1 hit.
    InterProi IPR023608. Gln_gamma-glutamylTfrase_euk.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR002931. Transglutaminase-like.
    IPR008958. Transglutaminase_C.
    IPR013808. Transglutaminase_CS.
    IPR001102. Transglutaminase_N.
    [Graphical view ]
    PANTHERi PTHR11590. PTHR11590. 1 hit.
    Pfami PF00927. Transglut_C. 2 hits.
    PF01841. Transglut_core. 1 hit.
    PF00868. Transglut_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000459. TGM_EBP42. 1 hit.
    SMARTi SM00460. TGc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49309. SSF49309. 2 hits.
    SSF81296. SSF81296. 1 hit.
    PROSITEi PS00547. TRANSGLUTAMINASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Transglutaminase type 1 and its cross-linking activity are concentrated at adherens junctions in simple epithelial cells."
      Hiiragi T., Sasaki H., Nagafuchi A., Sabe H., Shen S.C., Matsuki M., Yamanishi K., Tsukita S.
      J. Biol. Chem. 274:34148-34154(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION, TISSUE SPECIFICITY.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Kidney.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-91; SER-94 AND SER-803, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    4. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
      Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
      J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiTGM1_MOUSE
    AccessioniPrimary (citable) accession number: Q9JLF6
    Secondary accession number(s): Q8R0T9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 4, 2005
    Last sequence update: January 4, 2005
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3