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Protein

Nectin-3

Gene

Nectin3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectins-like or other nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.5 Publications

GO - Molecular functioni

GO - Biological processi

  • cell adhesion Source: MGI
  • cell recognition Source: GO_Central
  • fertilization Source: MGI
  • heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: GO_Central
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: HGNC
  • lens morphogenesis in camera-type eye Source: MGI
  • protein localization to cell junction Source: MGI
  • retina morphogenesis in camera-type eye Source: MGI
  • single organismal cell-cell adhesion Source: MGI
  • spermatid development Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-418990. Adherens junctions interactions.
R-MMU-420597. Nectin/Necl trans heterodimerization.

Names & Taxonomyi

Protein namesi
Recommended name:
Nectin-3
Alternative name(s):
Nectin cell adhesion molecule 3By similarity
Poliovirus receptor-related protein 3
CD_antigen: CD113
Gene namesi
Name:Nectin3By similarity
Synonyms:Pvrl3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1930171. Nectin3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini58 – 404ExtracellularSequence analysisAdd BLAST347
Transmembranei405 – 425HelicalSequence analysisAdd BLAST21
Topological domaini426 – 549CytoplasmicSequence analysisAdd BLAST124

GO - Cellular componenti

  • apical junction complex Source: MGI
  • cell-cell adherens junction Source: MGI
  • cell-cell contact zone Source: MGI
  • cell-cell junction Source: MGI
  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: HGNC
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Mice show an ocular phenotype, microphthalmia, accompanied by a separation of the contact between the pigment and non-pigment cell layers of the ciliary epithelia. Male mice exhibits infertility, suggesting a role in spermatogenesis. In the hippocampus, the formation and the number of adherens junctions at the synapses is impaired, and the trajectory of mossy fiber is abnormal.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 57Sequence analysisAdd BLAST57
ChainiPRO_000022637358 – 549Nectin-3Add BLAST492

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi73N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi78 ↔ 148PROSITE-ProRule annotation
Glycosylationi83N-linked (GlcNAc...)Sequence analysis1
Glycosylationi125N-linked (GlcNAc...)Sequence analysis1
Glycosylationi186N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi193 ↔ 246PROSITE-ProRule annotation
Glycosylationi222N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi291 ↔ 338PROSITE-ProRule annotation
Glycosylationi331N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9JLB9.
PeptideAtlasiQ9JLB9.
PRIDEiQ9JLB9.

PTM databases

iPTMnetiQ9JLB9.
PhosphoSitePlusiQ9JLB9.

Expressioni

Tissue specificityi

Ubiquitous with high expression in testes. Localized in spermatids at Sertoli-spermatid junctions. Expressed in ovarian granulosa cells, but only faintly expressed after ovulation.2 Publications

Gene expression databases

BgeeiENSMUSG00000022656.
CleanExiMM_PVRL3.
ExpressionAtlasiQ9JLB9. baseline and differential.
GenevisibleiQ9JLB9. MM.

Interactioni

Subunit structurei

Cis- and trans-homodimer. Can form trans-heterodimers with NECTIN1, NECTIN2, PVR, IGSF4B/Necl-1 and with IGSF4. Interaction between NECTIN1 and NECTIN3 on the pre- and postsynaptic sites, respectively, initiates the formation of puncta adherentia junctions between axons and dendrites. Interacts (via Cytoplasmic domain) with AFDN, providing a connection with the actin cytoskeleton. Binds with low affinity to TIGIT.5 Publications

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-41729N.
IntActiQ9JLB9. 2 interactors.
MINTiMINT-254672.
STRINGi10090.ENSMUSP00000023334.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AXAX-ray2.78A/B544-549[»]
ProteinModelPortaliQ9JLB9.
SMRiQ9JLB9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 165Ig-like V-typeAdd BLAST108
Domaini170 – 258Ig-like C2-type 1Add BLAST89
Domaini269 – 354Ig-like C2-type 2Add BLAST86

Sequence similaritiesi

Belongs to the nectin family.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGR7. Eukaryota.
ENOG410ZRVN. LUCA.
GeneTreeiENSGT00770000120512.
HOGENOMiHOG000115805.
HOVERGENiHBG082234.
InParanoidiQ9JLB9.
KOiK06592.
OMAiDVPFKQT.
OrthoDBiEOG091G0BLQ.
PhylomeDBiQ9JLB9.
TreeFamiTF331051.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR033319. Nectin-3.
[Graphical view]
PANTHERiPTHR23277:SF12. PTHR23277:SF12. 1 hit.
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 3 hits.
PROSITEiPS50835. IG_LIKE. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JLB9-1) [UniParc]FASTAAdd to basket
Also known as: Nectin-3 alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARTPGPAPL CPGGGKAQLS SAFPPAAGLL LPAPTPPPLL LLLIPLLLFS
60 70 80 90 100
RLCGALAGSI IVEPHVTAVW GKNVSLKCLI EVNETITQIS WEKIHGKSTQ
110 120 130 140 150
TVAVHHPQYG FSVQGDYQGR VLFKNYSLND ATITLHNIGF SDSGKYICKA
160 170 180 190 200
VTFPLGNAQS STTVTVLVEP TVSLIKGPDS LIDGGNETVA AVCVAATGKP
210 220 230 240 250
VAQIDWEGDL GEMESSTTSF PNETATIVSQ YKLFPTRFAR GRRITCVVKH
260 270 280 290 300
PALEKDIRYS FILDIQYAPE VSVTGYDGNW FVGRKGVNLK CNADANPPPF
310 320 330 340 350
KSVWSRLDGQ WPDGLLASDN TLHFVHPLTV NYSGVYVCKV SNSLGQRSDQ
360 370 380 390 400
KVIYISDPPT TTTLQPTVQW HSSPADVQDI ATEHKKLPFP LSTLATLKDD
410 420 430 440 450
TIGTIIASVV GGALFLVLVS ILAGVFCYRR RRTFRGDYFA KNYIPPSDMQ
460 470 480 490 500
KESQIDVLHQ DELDSYPDSV KKENKNPVNN LIRKDYLEEP EKTQWNNVEN
510 520 530 540
LTRFERPMDY YEDLKMGMKF VSDERYNESE DGLVSHVDGS VISRREWYV
Length:549
Mass (Da):60,583
Last modified:October 1, 2000 - v1
Checksum:i5492C9ABB472F185
GO
Isoform 2 (identifier: Q9JLB9-2) [UniParc]FASTAAdd to basket
Also known as: Nectin-3 beta

The sequence of this isoform differs from the canonical sequence as follows:
     358-510: PPTTTTLQPT...LTRFERPMDY → IPLTQTSSIA...LYINPREHYV
     511-549: Missing.

Show »
Length:510
Mass (Da):55,811
Checksum:i45CFE6EF78454864
GO
Isoform 3 (identifier: Q9JLB9-3) [UniParc]FASTAAdd to basket
Also known as: Nectin-3 gamma

The sequence of this isoform differs from the canonical sequence as follows:
     358-438: PPTTTTLQPT...RRRRTFRGDY → IPLTQTSSIA...LGQVRALEDT
     439-549: Missing.

Show »
Length:438
Mass (Da):47,262
Checksum:i2A0A4416E5B02FEF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti181L → S in BAB27933 (PubMed:16141072).Curated1
Sequence conflicti195 – 196AA → SS in BAB27933 (PubMed:16141072).Curated2
Sequence conflicti213 – 221MESSTTSFP → REFSTISFL in BAB27933 (PubMed:16141072).Curated9
Sequence conflicti232K → E in BAB27933 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017437358 – 510PPTTT…RPMDY → IPLTQTSSIAVAGAVIGAVL ALFIITVFVTVLLTPRKKRP SYLDKVIDLPPTHKPPPVYE ERIPSLPQKDLLGQTEHLPL QTQFKEKGAGGLQPSNGPIS RRFDYEDESTMQEDGTQRMC PLYSQMCHQDRSPRQHHPRN PERLYINPREHYV in isoform 2. 1 PublicationAdd BLAST153
Alternative sequenceiVSP_017438358 – 438PPTTT…FRGDY → IPLTQTSSIAVAGAVIGAVL ALFIITVFVTVLLTPRKKRP SYLDKVIDLPPTHKPPPVYE ERIPSLPQKDLLGQVRALED T in isoform 3. 2 PublicationsAdd BLAST81
Alternative sequenceiVSP_017439439 – 549Missing in isoform 3. 2 PublicationsAdd BLAST111
Alternative sequenceiVSP_017440511 – 549Missing in isoform 2. 1 PublicationAdd BLAST39

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195833 mRNA. Translation: AAF63685.1.
AF195834 mRNA. Translation: AAF63686.1.
AF195835 mRNA. Translation: AAF63687.1.
AK011949 mRNA. Translation: BAB27933.1.
BC125588 mRNA. Translation: AAI25589.1.
BC132187 mRNA. Translation: AAI32188.1.
CCDSiCCDS28204.1. [Q9JLB9-2]
CCDS28205.1. [Q9JLB9-3]
CCDS28206.1. [Q9JLB9-1]
RefSeqiNP_067470.1. NM_021495.4. [Q9JLB9-1]
NP_067471.1. NM_021496.3. [Q9JLB9-2]
NP_067472.1. NM_021497.2. [Q9JLB9-3]
UniGeneiMm.328072.
Mm.40477.

Genome annotation databases

EnsembliENSMUST00000023334; ENSMUSP00000023334; ENSMUSG00000022656. [Q9JLB9-1]
ENSMUST00000023335; ENSMUSP00000023335; ENSMUSG00000022656. [Q9JLB9-2]
ENSMUST00000096052; ENSMUSP00000093757; ENSMUSG00000022656. [Q9JLB9-3]
GeneIDi58998.
KEGGimmu:58998.
UCSCiuc007zji.3. mouse. [Q9JLB9-2]
uc007zjj.2. mouse. [Q9JLB9-3]
uc007zjk.3. mouse. [Q9JLB9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195833 mRNA. Translation: AAF63685.1.
AF195834 mRNA. Translation: AAF63686.1.
AF195835 mRNA. Translation: AAF63687.1.
AK011949 mRNA. Translation: BAB27933.1.
BC125588 mRNA. Translation: AAI25589.1.
BC132187 mRNA. Translation: AAI32188.1.
CCDSiCCDS28204.1. [Q9JLB9-2]
CCDS28205.1. [Q9JLB9-3]
CCDS28206.1. [Q9JLB9-1]
RefSeqiNP_067470.1. NM_021495.4. [Q9JLB9-1]
NP_067471.1. NM_021496.3. [Q9JLB9-2]
NP_067472.1. NM_021497.2. [Q9JLB9-3]
UniGeneiMm.328072.
Mm.40477.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AXAX-ray2.78A/B544-549[»]
ProteinModelPortaliQ9JLB9.
SMRiQ9JLB9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-41729N.
IntActiQ9JLB9. 2 interactors.
MINTiMINT-254672.
STRINGi10090.ENSMUSP00000023334.

PTM databases

iPTMnetiQ9JLB9.
PhosphoSitePlusiQ9JLB9.

Proteomic databases

PaxDbiQ9JLB9.
PeptideAtlasiQ9JLB9.
PRIDEiQ9JLB9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023334; ENSMUSP00000023334; ENSMUSG00000022656. [Q9JLB9-1]
ENSMUST00000023335; ENSMUSP00000023335; ENSMUSG00000022656. [Q9JLB9-2]
ENSMUST00000096052; ENSMUSP00000093757; ENSMUSG00000022656. [Q9JLB9-3]
GeneIDi58998.
KEGGimmu:58998.
UCSCiuc007zji.3. mouse. [Q9JLB9-2]
uc007zjj.2. mouse. [Q9JLB9-3]
uc007zjk.3. mouse. [Q9JLB9-1]

Organism-specific databases

CTDi25945.
MGIiMGI:1930171. Nectin3.

Phylogenomic databases

eggNOGiENOG410IGR7. Eukaryota.
ENOG410ZRVN. LUCA.
GeneTreeiENSGT00770000120512.
HOGENOMiHOG000115805.
HOVERGENiHBG082234.
InParanoidiQ9JLB9.
KOiK06592.
OMAiDVPFKQT.
OrthoDBiEOG091G0BLQ.
PhylomeDBiQ9JLB9.
TreeFamiTF331051.

Enzyme and pathway databases

ReactomeiR-MMU-418990. Adherens junctions interactions.
R-MMU-420597. Nectin/Necl trans heterodimerization.

Miscellaneous databases

PROiQ9JLB9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022656.
CleanExiMM_PVRL3.
ExpressionAtlasiQ9JLB9. baseline and differential.
GenevisibleiQ9JLB9. MM.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR033319. Nectin-3.
[Graphical view]
PANTHERiPTHR23277:SF12. PTHR23277:SF12. 1 hit.
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 3 hits.
PROSITEiPS50835. IG_LIKE. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNECT3_MOUSE
AccessioniPrimary (citable) accession number: Q9JLB9
Secondary accession number(s): Q059N7
, Q9D006, Q9JLB7, Q9JLB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.