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Protein

Neuronal acetylcholine receptor subunit alpha-10

Gene

Chrna10

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this leads to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma.3 Publications

GO - Molecular functioni

  • acetylcholine-activated cation-selective channel activity Source: RGD
  • calcium channel activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-10
Alternative name(s):
Nicotinic acetylcholine receptor subunit alpha-10
Short name:
NACHR alpha-10
Gene namesi
Name:Chrna10
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620142. Chrna10.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 237213ExtracellularSequence analysisAdd
BLAST
Transmembranei238 – 25821HelicalSequence analysisAdd
BLAST
Transmembranei268 – 28821HelicalSequence analysisAdd
BLAST
Transmembranei302 – 32221HelicalSequence analysisAdd
BLAST
Topological domaini323 – 425103CytoplasmicSequence analysisAdd
BLAST
Transmembranei426 – 44621HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • acetylcholine-gated channel complex Source: RGD
  • axon Source: RGD
  • cell junction Source: UniProtKB-KW
  • cytosol Source: Ensembl
  • perikaryon Source: RGD
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2094110.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 447423Neuronal acetylcholine receptor subunit alpha-10PRO_0000000377Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi40 – 401N-linked (GlcNAc...)Sequence analysis
Glycosylationi56 – 561N-linked (GlcNAc...)Sequence analysis
Disulfide bondi154 ↔ 168By similarity
Disulfide bondi218 ↔ 219Associated with receptor activationBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9JLB5.
PRIDEiQ9JLB5.

Expressioni

Tissue specificityi

Expressed in the outer hair cells of the cochlea and the neurons of dorsal root ganglia.2 Publications

Developmental stagei

Expression in the inner hair cells of the ear is lost at the onset of hearing, around P12. This correlates with a loss of sensitivity of these cells to cholinergic stimuli.2 Publications

Gene expression databases

GenevisibleiQ9JLB5. RN.

Interactioni

Subunit structurei

Forms heterooligomeric channels in conjunction with CHRNA9. The native outer hair cell receptor may be composed of CHRNA9-CHRNA10 heterooligomers.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027506.

Chemistry

BindingDBiQ9JLB5.

Structurei

3D structure databases

ProteinModelPortaliQ9JLB5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00790000122957.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiQ9JLB5.
KOiK04811.
OMAiAQRCHED.
OrthoDBiEOG72JWGV.
PhylomeDBiQ9JLB5.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JLB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTRSHYLDL GFLLLLFLPA ECLGAEGRLA HKLFRDLFAN YTSALRPVAD
60 70 80 90 100
TDQTLNVTLE VTLSQIIDMD ERNQVLTLYL WIRQEWTDAY LHWDPKAYGD
110 120 130 140 150
LDAIRIPSRL VWRPDIVLYN KADTQPPASA STNVVVRHDG AVRWDAPAIT
160 170 180 190 200
RSSCRVDVSA FPFDAQRCGL TFGSWTHGGH QLDVRPRGTS ASLADFVENV
210 220 230 240 250
EWRVLGMPAR RRVLTYGCCS EPYPDVTFTL LLRRRAAAYV CNLLLPCVFI
260 270 280 290 300
SLLAPLAFHL PADSGEKVSL GVTVLLALTV FQLILAESMP PAESVPLIGK
310 320 330 340 350
YYMATMTMVT FSTALTILIM NLHYCGPNAH PVPAWARVLL LGHLAKGLCV
360 370 380 390 400
RERGEPCGQS KPLESAPSLQ PPPASPAGPC HEPRCLCHQE ALLHHIASIA
410 420 430 440
STFRSHRAAQ RRHEDWKRLA RVMDRFFLGI FFCMALVMSL IVLVQAL
Length:447
Mass (Da):49,820
Last modified:October 1, 2000 - v1
Checksum:iEEE49D93490B698F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF196344 mRNA. Translation: AAF27624.1.
RefSeqiNP_072161.1. NM_022639.1.
UniGeneiRn.48767.

Genome annotation databases

EnsembliENSRNOT00000027507; ENSRNOP00000027506; ENSRNOG00000020293.
GeneIDi64574.
KEGGirno:64574.
UCSCiRGD:620142. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF196344 mRNA. Translation: AAF27624.1.
RefSeqiNP_072161.1. NM_022639.1.
UniGeneiRn.48767.

3D structure databases

ProteinModelPortaliQ9JLB5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027506.

Chemistry

BindingDBiQ9JLB5.
ChEMBLiCHEMBL2094110.

Proteomic databases

PaxDbiQ9JLB5.
PRIDEiQ9JLB5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027507; ENSRNOP00000027506; ENSRNOG00000020293.
GeneIDi64574.
KEGGirno:64574.
UCSCiRGD:620142. rat.

Organism-specific databases

CTDi57053.
RGDi620142. Chrna10.

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00790000122957.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiQ9JLB5.
KOiK04811.
OMAiAQRCHED.
OrthoDBiEOG72JWGV.
PhylomeDBiQ9JLB5.
TreeFamiTF315605.

Miscellaneous databases

PROiQ9JLB5.

Gene expression databases

GenevisibleiQ9JLB5. RN.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Alpha10: a determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells."
    Elgoyhen A.B., Vetter D.E., Katz E., Rothlin C.V., Heinemann S.F., Boulter J.
    Proc. Natl. Acad. Sci. U.S.A. 98:3501-3506(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CHRNA9, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Sprague-Dawley.
  2. "The alpha9alpha10 nicotinic acetylcholine receptor is permeable to and is modulated by divalent cations."
    Weisstaub N., Vetter D.E., Elgoyhen A.B., Katz E.
    Hear. Res. 167:122-135(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CHRNA9.
  3. "Coexpression of alpha 9 and alpha 10 nicotinic acetylcholine receptors in rat dorsal root ganglion neurons."
    Lips K.S., Pfeil U., Kummer W.
    Neuroscience 115:1-5(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "Developmental regulation of nicotinic synapses on cochlear inner hair cells."
    Katz E., Elgoyhen A.B., Gomez-Casati M.E., Knipper M., Vetter D.E., Fuchs P.A., Glowatzki E.
    J. Neurosci. 24:7814-7820(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  5. "Pharmacological properties of alpha 9 alpha 10 nicotinic acetylcholine receptors revealed by heterologous expression of subunit chimeras."
    Baker E.R., Zwart R., Sher E., Millar N.S.
    Mol. Pharmacol. 65:453-460(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CHRNA9.

Entry informationi

Entry nameiACH10_RAT
AccessioniPrimary (citable) accession number: Q9JLB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The heterooligomeric receptor composed of CHRNA9 and CHRNA10 has an atypical pharmacological profile, binding several non-nicotinic ligands including strychnine (a glycine receptor antagonist) and atropine (a muscarinic acetylcholine receptor antagonist).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.