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Protein

MAGUK p55 subfamily member 5

Gene

Mpp5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton.1 Publication

GO - Molecular functioni

  • guanylate kinase activity Source: MGI
  • protein domain specific binding Source: MGI

GO - Biological processi

  • bicellular tight junction assembly Source: InterPro
  • establishment of epithelial cell polarity Source: InterPro
  • establishment of protein localization to plasma membrane Source: Ensembl
  • morphogenesis of an epithelial sheet Source: MGI
  • myelin assembly Source: Ensembl
  • peripheral nervous system myelin maintenance Source: BHF-UCL
  • plasma membrane organization Source: MGI
  • protein localization to myelin sheath abaxonal region Source: BHF-UCL
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-420029. Tight junction interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
MAGUK p55 subfamily member 5
Alternative name(s):
Protein associated with Lin-7 1
Gene namesi
Name:Mpp5
Synonyms:Pals1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1927339. Mpp5.

Subcellular locationi

GO - Cellular componenti

  • bicellular tight junction Source: UniProtKB-SubCell
  • cytoplasm Source: MGI
  • endomembrane system Source: UniProtKB-SubCell
  • extracellular exosome Source: MGI
  • integral component of membrane Source: MGI
  • lateral loop Source: BHF-UCL
  • myelin sheath adaxonal region Source: BHF-UCL
  • nucleoplasm Source: MGI
  • plasma membrane Source: MGI
  • protein complex Source: Ensembl
  • Schmidt-Lanterman incisure Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi32 – 321H → A: No effect on interaction with PARD6B. 1 Publication
Mutagenesisi33 – 331R → A: No effect on interaction with PARD6B. 1 Publication
Mutagenesisi34 – 341E → A: No effect on interaction with PARD6B. 1 Publication
Mutagenesisi36 – 361A → G: No effect on interaction with PARD6B. 1 Publication
Mutagenesisi37 – 371V → G: Prevents interaction with PARD6B. Does not affect localization to the tight junctions. 1 Publication
Mutagenesisi37 – 371V → I: No effect on interaction with PARD6B. 1 Publication
Mutagenesisi38 – 381D → A: Prevents interaction with PARD6B. 1 Publication
Mutagenesisi38 – 381D → E: No effect on interaction with PARD6B. 1 Publication
Mutagenesisi39 – 391C → A: No effect on interaction with PARD6B. 1 Publication
Mutagenesisi40 – 401P → A: No effect on interaction with PARD6B. 1 Publication
Mutagenesisi150 – 1501L → G: No effect on PARD6B interaction. Prevents interaction with INADL; when associated with G-154. 1 Publication
Mutagenesisi154 – 1541V → G: No effect on PARD6B interaction. Prevents interaction with INADL; when associated with G-150. 1 Publication
Mutagenesisi207 – 2071E → Q: No effect on interaction with LIN7C. 1 Publication
Mutagenesisi208 – 2081L → G: Prevents interaction with LIN7C. 1 Publication
Mutagenesisi212 – 2121L → G: Prevents interaction with LIN7C. 1 Publication
Mutagenesisi216 – 2161H → N: No effect on interaction with LIN7C. 1 Publication
Mutagenesisi221 – 2211L → G: Partially prevents interaction with LIN7C. 1 Publication
Mutagenesisi224 – 2241H → N: Partially prevents interaction with LIN7C. 1 Publication
Mutagenesisi225 – 2251D → N: Prevents interaction with LIN7C. 1 Publication
Mutagenesisi227 – 2271V → G: Prevents interaction with LIN7C. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 675675MAGUK p55 subfamily member 5PRO_0000094581Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphoserineBy similarity
Modified residuei25 – 251PhosphoserineCombined sources
Modified residuei83 – 831PhosphoserineBy similarity
Modified residuei84 – 841PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9JLB2.
PaxDbiQ9JLB2.
PRIDEiQ9JLB2.

PTM databases

iPTMnetiQ9JLB2.
PhosphoSiteiQ9JLB2.

Expressioni

Tissue specificityi

Expressed in retina, lung, kidney, and heart. In the brain, expressed in the dentate gyrus of hippocampus, striatum and cerebellum (at protein level). Expressed in placenta, kidney, brain, heart, skeletal muscles, lung, pancreas and liver.3 Publications

Gene expression databases

BgeeiENSMUSG00000021112.
ExpressionAtlasiQ9JLB2. baseline and differential.
GenevisibleiQ9JLB2. MM.

Interactioni

Subunit structurei

Heterodimer with MPP1. Interacts with MPP7. Forms a complex with CRB1 and MPP4. Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3 (By similarity). Interacts with CRB3, EZR, INADL, LIN7C, MPDZ, PARD6B and SC6A1.By similarity8 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207851. 8 interactions.
IntActiQ9JLB2. 3 interactions.
MINTiMINT-1206544.
STRINGi10090.ENSMUSP00000080683.

Structurei

Secondary structure

1
675
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi123 – 13513Combined sources
Helixi141 – 15515Combined sources
Helixi157 – 16913Combined sources
Beta strandi247 – 2493Combined sources
Beta strandi255 – 2617Combined sources
Beta strandi269 – 2735Combined sources
Beta strandi275 – 2839Combined sources
Helixi288 – 2925Combined sources
Beta strandi300 – 3045Combined sources
Helixi314 – 32310Combined sources
Beta strandi326 – 3338Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VA8NMR-A236-335[»]
1VF6X-ray2.10C/D123-180[»]
ProteinModelPortaliQ9JLB2.
SMRiQ9JLB2. Positions 119-232, 236-675.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JLB2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini120 – 17758L27 1PROSITE-ProRule annotationAdd
BLAST
Domaini179 – 23557L27 2PROSITE-ProRule annotationAdd
BLAST
Domaini256 – 33681PDZPROSITE-ProRule annotationAdd
BLAST
Domaini345 – 41773SH3PROSITE-ProRule annotationAdd
BLAST
Domaini479 – 660182Guanylate kinase-likePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 140120Interaction with PARD6BAdd
BLAST
Regioni181 – 24363Interaction with LIN7CAdd
BLAST

Domaini

The L27 domain 1 functions in targeting to the tight junctions by binding to INADL.
The PDZ domain binds to the C-terminus of SC6A1.

Sequence similaritiesi

Belongs to the MAGUK family.Curated
Contains 1 guanylate kinase-like domain.PROSITE-ProRule annotation
Contains 2 L27 domains.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG0609. Eukaryota.
COG0194. LUCA.
GeneTreeiENSGT00760000118866.
HOGENOMiHOG000233034.
HOVERGENiHBG001858.
InParanoidiQ9JLB2.
KOiK06091.
OMAiHQKEGQE.
OrthoDBiEOG091G065I.
PhylomeDBiQ9JLB2.
TreeFamiTF314263.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR020590. Guanylate_kinase_CS.
IPR014775. L27_C.
IPR004172. L27_dom.
IPR015145. L27_N.
IPR031185. MPP5.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23122:SF14. PTHR23122:SF14. 1 hit.
PfamiPF00625. Guanylate_kin. 1 hit.
PF02828. L27. 1 hit.
PF09060. L27_N. 1 hit.
PF00595. PDZ. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
SM00569. L27. 2 hits.
SM00228. PDZ. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF101288. SSF101288. 2 hits.
SSF50044. SSF50044. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS51022. L27. 2 hits.
PS50106. PDZ. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JLB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSYMNGHV TEESDSGIKN LDLASPEEYP KHREMAVDCP GDLGTRMMPV
60 70 80 90 100
RRSAQLERIR QQQEDMRRRR EEEGKKQELD LNSSMRLKKL AQIPPKTGID
110 120 130 140 150
NPIFDTEEGI VLESPHYAVN ILDVEDLFSS LKHIQHTLVD SQSQEDISLL
160 170 180 190 200
LQLVQNRDFQ NAFKIHNAVT VHMSKASPPF PLIANVQDLV QEVQTVLKPV
210 220 230 240 250
HQKEGQELTA LLNAPHIQAL LLAHDKVAEQ EMQLEPITDE RVYESIGHYG
260 270 280 290 300
GETVKIVRIE KARDIPLGAT VRNEMDSVII SRIVKGGAAE KSGLLHEGDE
310 320 330 340 350
VLEINGIEIR GKDVNEVFDL LSDMHGTLTF VLIPSQQIKP PPAKETVIHV
360 370 380 390 400
KAHFDYDPSD DPYVPCRELG LSFQKGDILH VISQEDPNWW QAYREGDEDN
410 420 430 440 450
QPLAGLVPGK SFQQQREAMK QTIEEDKEPE KSGKLWCAKK NKKKRKKVLY
460 470 480 490 500
NANKNDDYDN EEILTYEEMS LYHQPANRKR PIILIGPQNC GQNELRQRLM
510 520 530 540 550
NKEKDRFASA VPHTTRNRRD HEVAGRDYHF VSRQAFEADI AAGKFIEHGE
560 570 580 590 600
FEKNLYGTSI DSVRQVINSG KICLLSLRTQ SLKTLRNSDL KPYIIFIAPP
610 620 630 640 650
SQERLRALLA KEGKNPKPEE LREIIEKTRE MEQNNGHYFD TAIVNSDLDK
660 670
AYQELLRLIN KLDTEPQWVP STWLR
Length:675
Mass (Da):77,229
Last modified:October 1, 2000 - v1
Checksum:i66670F919F18063B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF199008 mRNA. Translation: AAF63789.1.
CCDSiCCDS26001.1.
RefSeqiNP_062525.1. NM_019579.3.
XP_006516160.1. XM_006516097.2.
UniGeneiMm.425777.

Genome annotation databases

EnsembliENSMUST00000082024; ENSMUSP00000080683; ENSMUSG00000021112.
GeneIDi56217.
KEGGimmu:56217.
UCSCiuc007nzg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF199008 mRNA. Translation: AAF63789.1.
CCDSiCCDS26001.1.
RefSeqiNP_062525.1. NM_019579.3.
XP_006516160.1. XM_006516097.2.
UniGeneiMm.425777.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VA8NMR-A236-335[»]
1VF6X-ray2.10C/D123-180[»]
ProteinModelPortaliQ9JLB2.
SMRiQ9JLB2. Positions 119-232, 236-675.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207851. 8 interactions.
IntActiQ9JLB2. 3 interactions.
MINTiMINT-1206544.
STRINGi10090.ENSMUSP00000080683.

PTM databases

iPTMnetiQ9JLB2.
PhosphoSiteiQ9JLB2.

Proteomic databases

MaxQBiQ9JLB2.
PaxDbiQ9JLB2.
PRIDEiQ9JLB2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000082024; ENSMUSP00000080683; ENSMUSG00000021112.
GeneIDi56217.
KEGGimmu:56217.
UCSCiuc007nzg.2. mouse.

Organism-specific databases

CTDi64398.
MGIiMGI:1927339. Mpp5.

Phylogenomic databases

eggNOGiKOG0609. Eukaryota.
COG0194. LUCA.
GeneTreeiENSGT00760000118866.
HOGENOMiHOG000233034.
HOVERGENiHBG001858.
InParanoidiQ9JLB2.
KOiK06091.
OMAiHQKEGQE.
OrthoDBiEOG091G065I.
PhylomeDBiQ9JLB2.
TreeFamiTF314263.

Enzyme and pathway databases

ReactomeiR-MMU-420029. Tight junction interactions.

Miscellaneous databases

EvolutionaryTraceiQ9JLB2.
PROiQ9JLB2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021112.
ExpressionAtlasiQ9JLB2. baseline and differential.
GenevisibleiQ9JLB2. MM.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR020590. Guanylate_kinase_CS.
IPR014775. L27_C.
IPR004172. L27_dom.
IPR015145. L27_N.
IPR031185. MPP5.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23122:SF14. PTHR23122:SF14. 1 hit.
PfamiPF00625. Guanylate_kin. 1 hit.
PF02828. L27. 1 hit.
PF09060. L27_N. 1 hit.
PF00595. PDZ. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
SM00569. L27. 2 hits.
SM00228. PDZ. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF101288. SSF101288. 2 hits.
SSF50044. SSF50044. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS51022. L27. 2 hits.
PS50106. PDZ. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPP5_MOUSE
AccessioniPrimary (citable) accession number: Q9JLB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.