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Protein

High mobility group nucleosome-binding domain-containing protein 5

Gene

Hmgn5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction.2 Publications

GO - Molecular functioni

  • chromatin binding Source: MGI
  • DNA binding Source: UniProtKB-KW
  • poly(A) RNA binding Source: Ensembl

GO - Biological processi

  • chromatin modification Source: MGI
  • glutathione metabolic process Source: MGI
  • negative regulation of apoptotic process Source: Ensembl
  • negative regulation of cell cycle arrest Source: Ensembl
  • positive regulation of cell proliferation Source: Ensembl
  • positive regulation of gene expression Source: Ensembl
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
High mobility group nucleosome-binding domain-containing protein 5
Alternative name(s):
Nucleosome-binding protein 1
Nucleosome-binding protein 45
Short name:
NBP-45
Protein GARP45
Gene namesi
Name:Hmgn5
Synonyms:Garp45, Nsbp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1355295. Hmgn5.

Subcellular locationi

GO - Cellular componenti

  • chromatin Source: InterPro
  • nucleolus Source: Ensembl
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi18 – 181S → E: May abolish association with nucleosomes; when associated with E-22. 1 Publication
Mutagenesisi22 – 221S → E: May abolish association with nucleosomes; when associated with E-18. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 406406High mobility group nucleosome-binding domain-containing protein 5PRO_0000206718Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei29 – 291PhosphothreonineBy similarity
Cross-linki64 – 64Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki98 – 98Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki121 – 121Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9JL35.
MaxQBiQ9JL35.
PaxDbiQ9JL35.
PRIDEiQ9JL35.

PTM databases

iPTMnetiQ9JL35.
PhosphoSiteiQ9JL35.

Expressioni

Tissue specificityi

Expressed in liver, spleen, lung, heart, kidney, muscle and brain (at protein level). Widely expressed with highest levels in submaxillary gland, thymus, kidney and liver and lowest levels in brain, lung, pancreas and eye.2 Publications

Developmental stagei

At E7.5, expression is detected in the ectoplacental cone but not in embryonic tissues. By E9.5 and E12.5, strongly expressed in the giant trophoblast, spongiotrophoblast and decidual cells of the placenta (at protein level). At E9.5 and E11.5, weakly expressed in the developing embryo.1 Publication

Gene expression databases

BgeeiQ9JL35.
CleanExiMM_NSBP1.
GenevisibleiQ9JL35. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9JL35. 1 interaction.
MINTiMINT-1849891.
STRINGi10090.ENSMUSP00000033597.

Family & Domainsi

Domaini

Specifically targeted by its C-terminus to nucleosomes in euchromatin.1 Publication

Sequence similaritiesi

Belongs to the HMGN family.Curated

Phylogenomic databases

eggNOGiENOG410JCH1. Eukaryota.
ENOG410XQBG. LUCA.
GeneTreeiENSGT00730000111570.
HOGENOMiHOG000070450.
HOVERGENiHBG052673.
InParanoidiQ9JL35.
KOiK17045.
OMAiEYNNENA.
OrthoDBiEOG77T177.
PhylomeDBiQ9JL35.
TreeFamiTF105374.

Family and domain databases

InterProiIPR000079. HMGN_fam.
[Graphical view]
PfamiPF01101. HMG14_17. 1 hit.
[Graphical view]
PRINTSiPR00925. NONHISHMG17.
SMARTiSM00527. HMG17. 1 hit.
[Graphical view]
PROSITEiPS00355. HMG14_17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JL35-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKRKAAGDV SQEPKRRSAR LSAMPVPFTP ELKPKRASTS RKTKTTNVVE
60 70 80 90 100
ENKDASTIPI PETKPEDVKD ECNMENAENG EAKIMEAPIP KMEAEEVKEQ
110 120 130 140 150
INEDTEEDGG EKKEAVAAEA KDDELKANIQ DVEKDEDGKE HKDTGEEVED
160 170 180 190 200
GKIEEEGLNE KPGTAKSEDA EVSKDEEEKG DNEKGEDGKE EGDEKEEEKD
210 220 230 240 250
DKEGDTGTEK EVKEQNKEAE EDDGKCKEEE NKEVGKEGQP EEDGKEDLHE
260 270 280 290 300
EVGKEDLHEE DGKEGQPEED GKEIHHEEDG KEGQPEEDGK EYLHEEDGEE
310 320 330 340 350
GQPKEDQKEG QPEEDGKEDQ PEEDGKEGQC KEDGKEGHHE EGGKEDLHEE
360 370 380 390 400
DGKEKDGGKE DRKEEGEQEV AVDEGSDENK VEAEEEGAEN KDFKQDGEKE

EPLSIV
Length:406
Mass (Da):45,344
Last modified:September 19, 2002 - v2
Checksum:i59A4305613EC9679
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti74 – 741M → V in AAF30179 (PubMed:10692437).Curated
Sequence conflicti390 – 3901N → H in BAA33783 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF213454 mRNA. Translation: AAF30179.1.
AB018374 mRNA. Translation: BAA33783.2.
AK013748 mRNA. Translation: BAB28982.2.
AK131996 mRNA. Translation: BAE20926.1.
AL954348 Genomic DNA. Translation: CAM15237.1.
BC021626 mRNA. Translation: AAH21626.1.
BC083087 mRNA. Translation: AAH83087.1.
CCDSiCCDS41101.1.
RefSeqiNP_057919.2. NM_016710.2.
UniGeneiMm.298443.

Genome annotation databases

EnsembliENSMUST00000033597; ENSMUSP00000033597; ENSMUSG00000031245.
GeneIDi50887.
KEGGimmu:50887.
UCSCiuc009ucs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF213454 mRNA. Translation: AAF30179.1.
AB018374 mRNA. Translation: BAA33783.2.
AK013748 mRNA. Translation: BAB28982.2.
AK131996 mRNA. Translation: BAE20926.1.
AL954348 Genomic DNA. Translation: CAM15237.1.
BC021626 mRNA. Translation: AAH21626.1.
BC083087 mRNA. Translation: AAH83087.1.
CCDSiCCDS41101.1.
RefSeqiNP_057919.2. NM_016710.2.
UniGeneiMm.298443.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JL35. 1 interaction.
MINTiMINT-1849891.
STRINGi10090.ENSMUSP00000033597.

PTM databases

iPTMnetiQ9JL35.
PhosphoSiteiQ9JL35.

Proteomic databases

EPDiQ9JL35.
MaxQBiQ9JL35.
PaxDbiQ9JL35.
PRIDEiQ9JL35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033597; ENSMUSP00000033597; ENSMUSG00000031245.
GeneIDi50887.
KEGGimmu:50887.
UCSCiuc009ucs.1. mouse.

Organism-specific databases

CTDi79366.
MGIiMGI:1355295. Hmgn5.

Phylogenomic databases

eggNOGiENOG410JCH1. Eukaryota.
ENOG410XQBG. LUCA.
GeneTreeiENSGT00730000111570.
HOGENOMiHOG000070450.
HOVERGENiHBG052673.
InParanoidiQ9JL35.
KOiK17045.
OMAiEYNNENA.
OrthoDBiEOG77T177.
PhylomeDBiQ9JL35.
TreeFamiTF105374.

Miscellaneous databases

ChiTaRSiHmgn5. mouse.
NextBioi307873.
PROiQ9JL35.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JL35.
CleanExiMM_NSBP1.
GenevisibleiQ9JL35. MM.

Family and domain databases

InterProiIPR000079. HMGN_fam.
[Graphical view]
PfamiPF01101. HMG14_17. 1 hit.
[Graphical view]
PRINTSiPR00925. NONHISHMG17.
SMARTiSM00527. HMG17. 1 hit.
[Graphical view]
PROSITEiPS00355. HMG14_17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "NBP-45, a novel nucleosomal binding protein with a tissue-specific and developmentally regulated expression."
    Shirakawa H., Landsman D., Postnikov Y.V., Bustin M.
    J. Biol. Chem. 275:6368-6374(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. Onoda G., Suzuki N., Saito H., Honda T., Sato H., Kuwano R.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo and Hippocampus.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon and Limb.
  6. "The nucleosomal binding protein NSBP1 is highly expressed in the placenta and modulates the expression of differentiation markers in placental Rcho-1 cells."
    Shirakawa H., Rochman M., Furusawa T., Kuehn M.R., Horigome S., Haketa K., Sugita Y., Inada T., Komai M., Bustin M.
    J. Cell. Biochem. 106:651-658(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  7. "The interaction of NSBP1/HMGN5 with nucleosomes in euchromatin counteracts linker histone-mediated chromatin compaction and modulates transcription."
    Rochman M., Postnikov Y., Correll S., Malicet C., Wincovitch S., Karpova T.S., McNally J.G., Wu X., Bubunenko N.A., Grigoryev S., Bustin M.
    Mol. Cell 35:642-656(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DOMAIN, MUTAGENESIS OF SER-18 AND SER-22.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiHMGN5_MOUSE
AccessioniPrimary (citable) accession number: Q9JL35
Secondary accession number(s): O88832
, Q3V272, Q8VC71, Q9CUW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: September 19, 2002
Last modified: April 13, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.