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Protein

Galactoside 2-alpha-L-fucosyltransferase 2

Gene

Fut2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates the transfer of fucose to the terminal galactose on glycan chains of cell surface glycoproteins and glycolipids (PubMed:11323419, PubMed:27161092). The resulting epitope plays a role in cell-cell interaction including host-microbe interaction (PubMed:19706747, PubMed:27161092). Mediates interaction with intestinal microbiota influencing its composition (PubMed:19706747, PubMed:27161092). Creates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen, which is an essential substrate for the final step in the blood group antigen synthesis pathway (PubMed:11323419).3 Publications

Miscellaneous

In mouse, there are three genes (Fut1, Fut2 and Sec1) which encode galactoside 2-L-fucosyltransferase.1 Publication

Catalytic activityi

GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R.1 Publication
GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R.1 Publication

Kineticsi

  1. KM=10.2 mM for phenyl-beta-D-galactoside1 Publication

    Pathwayi: protein glycosylation

    This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 Publication
    View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

    GO - Molecular functioni

    • fucosyltransferase activity Source: MGI
    • galactoside 2-alpha-L-fucosyltransferase activity Source: MGI

    GO - Biological processi

    • carbohydrate metabolic process Source: InterPro
    • protein glycosylation Source: MGI

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.1.69 3474
    SABIO-RKQ9JL27
    UniPathwayiUPA00378

    Protein family/group databases

    CAZyiGT11 Glycosyltransferase Family 11

    Chemistry databases

    SwissLipidsiSLP:000001426

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Galactoside 2-alpha-L-fucosyltransferase 2 (EC:2.4.1.3441 Publication, EC:2.4.1.691 Publication)
    Alternative name(s):
    Alpha(1,2)FT 2
    Fucosyltransferase 2
    GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 2
    Secretory blood group protein 2
    Gene namesi
    Name:Fut2
    Synonyms:Sec2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 7

    Organism-specific databases

    MGIiMGI:109374 Fut2

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 5CytoplasmicSequence analysis5
    Transmembranei6 – 26Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
    Topological domaini27 – 347LumenalSequence analysisAdd BLAST321

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001491091 – 347Galactoside 2-alpha-L-fucosyltransferase 2Add BLAST347

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi192N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi258N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi286N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi312N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ9JL27
    PaxDbiQ9JL27
    PRIDEiQ9JL27

    PTM databases

    PhosphoSitePlusiQ9JL27

    Expressioni

    Tissue specificityi

    Expressed in stomach, colon, ovary and uterus, specifically in luminal uterine epithelium.1 Publication

    Developmental stagei

    Detected at highest levels in proestrus and estrus, with 10- to 12-fold increases over early diestrus.1 Publication

    Gene expression databases

    BgeeiENSMUSG00000055978
    CleanExiMM_FUT2
    ExpressionAtlasiQ9JL27 baseline and differential
    GenevisibleiQ9JL27 MM

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000063719

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JL27
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 11 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG410IFKR Eukaryota
    ENOG4111HG3 LUCA
    GeneTreeiENSGT00390000001450
    HOGENOMiHOG000261621
    HOVERGENiHBG004338
    InParanoidiQ9JL27
    KOiK00718
    OMAiAFLPEWT
    OrthoDBiEOG091G0X2P
    PhylomeDBiQ9JL27
    TreeFamiTF315810

    Family and domain databases

    InterProiView protein in InterPro
    IPR002516 Glyco_trans_11
    PANTHERiPTHR11927 PTHR11927, 1 hit
    PfamiView protein in Pfam
    PF01531 Glyco_transf_11, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9JL27-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MASAQVPFSF PLAHFLIFVF VTSTIIHLQQ RIVKLQTLSE KELQAVQMSS
    60 70 80 90 100
    PNAARTDMQQ SAKLQGIFTI NSIGRLGNQM GEYATLFALA RMNGRLAFIP
    110 120 130 140 150
    ESMHNALAPI FRISLPVLHS DTARRIPWQN YHLNDWMEER YRHIPGQYVR
    160 170 180 190 200
    FTGYPCSWTF YHHLRPEILK EFTLHDHVRE EAQAFLRGLR VNGSQPSTFV
    210 220 230 240 250
    GVHVRRGDYV HVMPKVWKGV VADRGYLEKA LDRFRARYSS PVFVVTSNGM
    260 270 280 290 300
    AWCRENINTS LGDVVFAGNG IEGSPAKDFA LLTQCNHTIM TIGTFGIWAA
    310 320 330 340
    YLAGGDTIYL ANYTLPDSPF LKIFKPAAAF LPEWMGIPAD LSPLLKH
    Length:347
    Mass (Da):39,243
    Last modified:October 1, 2000 - v1
    Checksum:iDD5F441046CC8E79
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF214656 Genomic DNA Translation: AAF45146.1
    AK033520 mRNA Translation: BAC28338.1
    CCDSiCCDS21258.1
    RefSeqiNP_001258922.1, NM_001271993.1
    NP_061364.2, NM_018876.4
    XP_011249097.1, XM_011250795.1
    UniGeneiMm.290046
    Mm.491300

    Genome annotation databases

    EnsembliENSMUST00000069800; ENSMUSP00000063719; ENSMUSG00000055978
    GeneIDi14344
    KEGGimmu:14344
    UCSCiuc009gwn.2 mouse

    Similar proteinsi

    Entry informationi

    Entry nameiFUT2_MOUSE
    AccessioniPrimary (citable) accession number: Q9JL27
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 6, 2003
    Last sequence update: October 1, 2000
    Last modified: April 25, 2018
    This is version 125 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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