Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Galactoside 2-alpha-L-fucosyltransferase 2

Gene

Fut2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the transfer of fucose to the terminal galactose on glycan chains of cell surface glycoproteins and glycolipids (PubMed:11323419, PubMed:27161092). The resulting epitope plays a role in cell-cell interaction including host-microbe interaction (PubMed:19706747, PubMed:27161092). Mediates interaction with intestinal microbiota influencing its composition (PubMed:19706747, PubMed:27161092). Creates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen, which is an essential substrate for the final step in the blood group antigen synthesis pathway (PubMed:11323419).3 Publications

Catalytic activityi

GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.1 Publication

Kineticsi

  1. KM=10.2 mM for phenyl-beta-D-galactoside1 Publication

    Pathwayi: protein glycosylation

    This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 Publication
    View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

    GO - Molecular functioni

    • fucosyltransferase activity Source: MGI
    • galactoside 2-alpha-L-fucosyltransferase activity Source: MGI

    GO - Biological processi

    • carbohydrate metabolic process Source: InterPro
    • protein glycosylation Source: MGI
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.1.69. 3474.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT11. Glycosyltransferase Family 11.

    Chemistry databases

    SwissLipidsiSLP:000001426.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Galactoside 2-alpha-L-fucosyltransferase 2 (EC:2.4.1.691 Publication)
    Alternative name(s):
    Alpha(1,2)FT 2
    Fucosyltransferase 2
    GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 2
    Secretory blood group protein 2
    Gene namesi
    Name:Fut2
    Synonyms:Sec2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 7

    Organism-specific databases

    MGIiMGI:109374. Fut2.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 5CytoplasmicSequence analysis5
    Transmembranei6 – 26Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
    Topological domaini27 – 347LumenalSequence analysisAdd BLAST321

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001491091 – 347Galactoside 2-alpha-L-fucosyltransferase 2Add BLAST347

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi192N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi258N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi286N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi312N-linked (GlcNAc...)Sequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ9JL27.
    PaxDbiQ9JL27.
    PRIDEiQ9JL27.

    PTM databases

    PhosphoSitePlusiQ9JL27.

    Expressioni

    Tissue specificityi

    Expressed in stomach, colon, ovary and uterus, specifically in luminal uterine epithelium.1 Publication

    Developmental stagei

    Detected at highest levels in proestrus and estrus, with 10- to 12-fold increases over early diestrus.1 Publication

    Gene expression databases

    BgeeiENSMUSG00000055978.
    CleanExiMM_FUT2.
    ExpressionAtlasiQ9JL27. baseline and differential.
    GenevisibleiQ9JL27. MM.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000063719.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JL27.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 11 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG410IFKR. Eukaryota.
    ENOG4111HG3. LUCA.
    GeneTreeiENSGT00390000001450.
    HOGENOMiHOG000261621.
    HOVERGENiHBG004338.
    InParanoidiQ9JL27.
    KOiK00718.
    OMAiAFLPEWT.
    OrthoDBiEOG091G0X2P.
    PhylomeDBiQ9JL27.
    TreeFamiTF315810.

    Family and domain databases

    InterProiIPR002516. Glyco_trans_11.
    [Graphical view]
    PANTHERiPTHR11927. PTHR11927. 1 hit.
    PfamiPF01531. Glyco_transf_11. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9JL27-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MASAQVPFSF PLAHFLIFVF VTSTIIHLQQ RIVKLQTLSE KELQAVQMSS
    60 70 80 90 100
    PNAARTDMQQ SAKLQGIFTI NSIGRLGNQM GEYATLFALA RMNGRLAFIP
    110 120 130 140 150
    ESMHNALAPI FRISLPVLHS DTARRIPWQN YHLNDWMEER YRHIPGQYVR
    160 170 180 190 200
    FTGYPCSWTF YHHLRPEILK EFTLHDHVRE EAQAFLRGLR VNGSQPSTFV
    210 220 230 240 250
    GVHVRRGDYV HVMPKVWKGV VADRGYLEKA LDRFRARYSS PVFVVTSNGM
    260 270 280 290 300
    AWCRENINTS LGDVVFAGNG IEGSPAKDFA LLTQCNHTIM TIGTFGIWAA
    310 320 330 340
    YLAGGDTIYL ANYTLPDSPF LKIFKPAAAF LPEWMGIPAD LSPLLKH
    Length:347
    Mass (Da):39,243
    Last modified:October 1, 2000 - v1
    Checksum:iDD5F441046CC8E79
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF214656 Genomic DNA. Translation: AAF45146.1.
    AK033520 mRNA. Translation: BAC28338.1.
    CCDSiCCDS21258.1.
    RefSeqiNP_001258922.1. NM_001271993.1.
    NP_061364.2. NM_018876.4.
    XP_011249097.1. XM_011250795.1.
    UniGeneiMm.290046.
    Mm.491300.

    Genome annotation databases

    EnsembliENSMUST00000069800; ENSMUSP00000063719; ENSMUSG00000055978.
    GeneIDi14344.
    KEGGimmu:14344.
    UCSCiuc009gwn.2. mouse.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    Fucosyltransferase 2

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF214656 Genomic DNA. Translation: AAF45146.1.
    AK033520 mRNA. Translation: BAC28338.1.
    CCDSiCCDS21258.1.
    RefSeqiNP_001258922.1. NM_001271993.1.
    NP_061364.2. NM_018876.4.
    XP_011249097.1. XM_011250795.1.
    UniGeneiMm.290046.
    Mm.491300.

    3D structure databases

    ProteinModelPortaliQ9JL27.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000063719.

    Chemistry databases

    SwissLipidsiSLP:000001426.

    Protein family/group databases

    CAZyiGT11. Glycosyltransferase Family 11.

    PTM databases

    PhosphoSitePlusiQ9JL27.

    Proteomic databases

    MaxQBiQ9JL27.
    PaxDbiQ9JL27.
    PRIDEiQ9JL27.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000069800; ENSMUSP00000063719; ENSMUSG00000055978.
    GeneIDi14344.
    KEGGimmu:14344.
    UCSCiuc009gwn.2. mouse.

    Organism-specific databases

    CTDi2524.
    MGIiMGI:109374. Fut2.

    Phylogenomic databases

    eggNOGiENOG410IFKR. Eukaryota.
    ENOG4111HG3. LUCA.
    GeneTreeiENSGT00390000001450.
    HOGENOMiHOG000261621.
    HOVERGENiHBG004338.
    InParanoidiQ9JL27.
    KOiK00718.
    OMAiAFLPEWT.
    OrthoDBiEOG091G0X2P.
    PhylomeDBiQ9JL27.
    TreeFamiTF315810.

    Enzyme and pathway databases

    UniPathwayiUPA00378.
    BRENDAi2.4.1.69. 3474.

    Miscellaneous databases

    PROiQ9JL27.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000055978.
    CleanExiMM_FUT2.
    ExpressionAtlasiQ9JL27. baseline and differential.
    GenevisibleiQ9JL27. MM.

    Family and domain databases

    InterProiIPR002516. Glyco_trans_11.
    [Graphical view]
    PANTHERiPTHR11927. PTHR11927. 1 hit.
    PfamiPF01531. Glyco_transf_11. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiFUT2_MOUSE
    AccessioniPrimary (citable) accession number: Q9JL27
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 6, 2003
    Last sequence update: October 1, 2000
    Last modified: November 30, 2016
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In mouse, there are three genes (Fut1, Fut2 and Sec1) which encode galactoside 2-L-fucosyltransferase.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.