Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nuclear receptor coactivator 6

Gene

Ncoa6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivates expression in an agonist- and AF2-dependent manner. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins. Involved in placental, cardiac, hepatic and embryonic development.

GO - Molecular functioni

GO - Biological processi

  • brain development Source: MGI
  • cellular response to DNA damage stimulus Source: MGI
  • heart development Source: MGI
  • histone H3-K4 trimethylation Source: MGI
  • labyrinthine layer blood vessel development Source: MGI
  • myeloid cell differentiation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: MGI
  • regulation of gene expression Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor coactivator 6
Alternative name(s):
Activating signal cointegrator 2
Short name:
ASC-2
Amplified in breast cancer protein 3
Cancer-amplified transcriptional coactivator ASC-2
Nuclear receptor coactivator RAP250
Short name:
NRC
Nuclear receptor-activating protein, 250 kDa
Peroxisome proliferator-activated receptor-interacting protein
Short name:
PPAR-interacting protein
Thyroid hormone receptor-binding protein
Gene namesi
Name:Ncoa6
Synonyms:Aib3, Prip, Rap250, Trbp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1929915. Ncoa6.

Subcellular locationi

GO - Cellular componenti

  • histone methyltransferase complex Source: MGI
  • nucleoplasm Source: Reactome
  • nucleus Source: CACAO
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi891 – 8944LVNL → AVNA: Abolishes interaction with nuclear receptors. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 20672067Nuclear receptor coactivator 6PRO_0000094414Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei888 – 8881PhosphoserineBy similarity
Modified residuei1325 – 13251PhosphothreonineBy similarity
Modified residuei1822 – 18221N6-acetyllysineCombined sources
Modified residuei1825 – 18251N6-acetyllysineCombined sources
Modified residuei2022 – 20221PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9JL19.
MaxQBiQ9JL19.
PaxDbiQ9JL19.
PRIDEiQ9JL19.

PTM databases

iPTMnetiQ9JL19.
PhosphoSiteiQ9JL19.

Expressioni

Tissue specificityi

Widely expressed. High expression in testis and weak expression in small intestine.

Developmental stagei

Expressed at E9 in placenta and at weaker level in uterus. High expression in neural tube and in CNS throughout development. High expression in sensory ganglia and retina from E11. In the alimentary tract and olfactory epithelium expression was seen from E13. Strong expression present in liver and kidney, from E11 and E13 respectively, and then expression decreased at later stages of development. Moderate expression in lung from E13, while it decreases during postnatal life. Strong expression in thymus from E15 onwards, and in spleen from E17 and during early postnatal life, then, the expression decreases.

Gene expression databases

CleanExiMM_NCOA6.

Interactioni

Subunit structurei

Monomer and homodimer. Interacts in vitro with the basal transcription factors GTF2A and TBP, suggesting an autonomous transactivation function. Interacts with NCOA1, CRSP3, RBM14, the histone acetyltransferase proteins EP300 and CREBBP, and with methyltransferase proteins NCOA6IP and PRMT2 (By similarity). Interacts with RBM39. Component of the MLL2/3 complex (also named ASCOM complex), at least composed of KMT2D/MLL2 or KMT2C/MLL3, ASH2L, RBBP5, WDR5, NCOA6, DPY30, KDM6A, PAXIP1/PTIP, PAGR1 and alpha- and beta-tubulin (By similarity). Interacts with ZNF335; may enhance ligand-dependent transcriptional activation by nuclear hormone receptors (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-61283N.
IntActiQ9JL19. 1 interaction.
MINTiMINT-5200950.
STRINGi10090.ENSMUSP00000045386.

Structurei

3D structure databases

ProteinModelPortaliQ9JL19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 13141314NCOA1-binding regionBy similarityAdd
BLAST
Regioni1 – 10601060CREBBP-binding regionBy similarityAdd
BLAST
Regioni1 – 932932TBP/GTF2A-binding regionBy similarityAdd
BLAST
Regioni777 – 931155NCOA6IP-binding regionBy similarityAdd
BLAST
Regioni1644 – 2067424EP300/CRSP3-binding regionBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi891 – 8955LXXLL motif 1
Motifi1495 – 14995LXXLL motif 2

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi227 – 1044818Gln-richAdd
BLAST
Compositional biasi376 – 3816Poly-Pro
Compositional biasi917 – 9226Poly-Lys
Compositional biasi1543 – 159250Ser-richAdd
BLAST

Domaini

Contains two Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Only motif 1 is essential for the association with nuclear receptors.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IK20. Eukaryota.
ENOG410XW4F. LUCA.
HOVERGENiHBG052586.
InParanoidiQ9JL19.

Family and domain databases

InterProiIPR026638. NCOA6.
IPR032715. NCOA6_nucleic_acid-bd.
[Graphical view]
PANTHERiPTHR15690:SF0. PTHR15690:SF0. 2 hits.
PfamiPF13820. Nucleic_acid_bd. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JL19-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLDDLPNFE DIYTSLCSST MGDSEVEFDS GLEDDDTKGD SILEDSTIFV
60 70 80 90 100
AFKGNIDDKD FKWKLDAILK NVPNLLHMES SKLKVQKVEP WNSVRVTFNI
110 120 130 140 150
PREAAERLWI LAQSNNQQLR DLGILSVQIE GEGAINLALG QNRSQDVRMN
160 170 180 190 200
GPVASGNSVR MEAGFPMASG PGLIRMTSPA AVMTPQGGNM SSSMMAPGPN
210 220 230 240 250
PELQPRTPRP ASQSDAMDPL LSGLHIQQQS HPSGSLPPAH HSMQPVPVNR
260 270 280 290 300
QMNPANFPQL QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ LQTRPLQQHQ
310 320 330 340 350
QQPQGIRPQF TAPTQVPVPP GWNQLPSGAL QPPPAQGSLG TMTTNQGWKK
360 370 380 390 400
APLPSPMQAQ LQARPSLATV QTPSHPPPPY PFGSQQASQA HTNFPQMSNP
410 420 430 440 450
GQFTAPQMKG LQGGPSRVPT PLQQPHLTNK SPASSPSSFQ QGSPASSPTV
460 470 480 490 500
NQTQQQMGPR PPQNNPLSQG FQQPVSSPGR NPMVQQGNVP PNFMVMQQQP
510 520 530 540 550
PNQGPQSLHP GLGGMPKRLP PGFSAGQANP NFMQGQVPST TAATPGNSGA
560 570 580 590 600
LQLQANQNVQ HAGGQGAGPP QNQMQVSHGP PNMMQPSLMG IHGNINNQQA
610 620 630 640 650
GSSGVPQVTL GNMQGQPQQG PPSQLMGMHQ QIVPSQGQMA QQQGTLNPQN
660 670 680 690 700
PMILSRAQLM PQGQMMVNAQ NQNLGPSPQR MTPPKQMLPQ QGPQMMAPHN
710 720 730 740 750
QMMGPQGQVL LQQNPMIEQI MTNQMQGNKA QFNSQNQSNV MPGPAQIMRG
760 770 780 790 800
PTPNMQGNMV QFTGQMSGQM LPQQGPVNNS PSQVMGIQGQ VLRPPGPSPH
810 820 830 840 850
MAQQHTDPVT TANNDVNLSQ MMPDVSMQQA SMVPPHVQSM QGNSASGSHF
860 870 880 890 900
SGHGVSFNAP FGGAPNGSQM SCGQNPGFPV NKDVTLTSPL LVNLLQSDIS
910 920 930 940 950
AGHFGVNNKQ NNTNANKPKK KKPPRKKKNC HQDLNTPDNR PTGLEEVDQQ
960 970 980 990 1000
SLPGEQGINL DTTGPKLPDF SNRPPGYPTQ PVEQRPLPQM PPQLMQHVAP
1010 1020 1030 1040 1050
PPQPPQQQPQ PQLPQQQQPP PPSQPQSQQQ QQQQQMMMML MMQQDPKSIR
1060 1070 1080 1090 1100
LPVSQNVHPP RGPLNPDSQR MPVQQSGNVP VMVGLQGPAS VPPSPDKQRM
1110 1120 1130 1140 1150
PMSVNTPMGS NSRKMVYQEN PQNSSSSPLG EMSSLPEASG SEVPSVAGGP
1160 1170 1180 1190 1200
NNMPSHLVVS QNQLMMTGPK PGPSPLSATQ GATPQQPPVN SLPSSHGHHF
1210 1220 1230 1240 1250
PNVAAPTQTS RPKTPNRASP RPYYPQTPNN RPPSTEPSEI SLSPERLNAS
1260 1270 1280 1290 1300
IAGLFPPQIN IPLPPRPNLN RGFDQQGLNP TTLKAIGQAP SNLTITNPPN
1310 1320 1330 1340 1350
FAAPQAHKLD SVVVNSGKQS NPGTTKRASP SNSRRSSPGS SRKTTPSPGR
1360 1370 1380 1390 1400
QNSKAPKLTL ASQTSTTMLQ NMELPRNVLV GPTPLANPPL PGSFPNNTGL
1410 1420 1430 1440 1450
NPQNPTVPVP AMGTVLEDNK ESVNIPQDSD CQNAQGRKEQ VNTELKVVPT
1460 1470 1480 1490 1500
QEAKMAVPED QSKKDGQPLD PNKLPSVEEN KNLMSPAMRE APTSLSQLLD
1510 1520 1530 1540 1550
NSGAPNVTIK PPGLTDLEVT PPVVSGEDLR KASVIPTLQD PPSKEPSTSL
1560 1570 1580 1590 1600
SSPHSSEPCS TLARSELSEV SSNAAPSIPP VMSRPVSSSS ISTPLPPNQI
1610 1620 1630 1640 1650
TVFVTSNPIT TSSNTSAALP THLQSALMST VVTMPNVGNK VMVSEGQSAA
1660 1670 1680 1690 1700
QSNARPQFIT PVFINSSSII QVMKGSQPST IPATPLTTNS GLMPPSVAVV
1710 1720 1730 1740 1750
GPLHIPQNIK FSSAPVTPNV PSSSPAPNIQ TGRPLVLSSR ATPVQLPSPP
1760 1770 1780 1790 1800
CTSSPVVAPN PSVQQVKELN PDEASPQTNT SADQSTLPPS QPTTVVSSLL
1810 1820 1830 1840 1850
TNSPGSSANR RSPVSSSKGK GKVDKIGQIL LTKACKKVTG SLEKGEEQYG
1860 1870 1880 1890 1900
ADGETEGPGL EITTPGLMGT EQCSTELDSK TPTPSAPTLL KMTSSPMAPS
1910 1920 1930 1940 1950
STSTGPILPG GALPTSVRSI VTTLVPSELI STAPTTKGNH GGVTSEPLAG
1960 1970 1980 1990 2000
GLVEEKVGSH PELLPSIAPS QNLAPKETPA TALQGSVARP ELEANAAIAS
2010 2020 2030 2040 2050
GQSCEPKEIV EKSKTLTSRR NSRTEEPTMA SESVENGHRK RSSRPASASS
2060
STKDITGAVQ SKRRKSK
Length:2,067
Mass (Da):219,663
Last modified:October 1, 2000 - v1
Checksum:iC855F8777167AD48
GO
Isoform 2 (identifier: Q9JL19-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     458-2067: Missing.

Note: Acts as a dominant negative repressor.
Show »
Length:457
Mass (Da):49,981
Checksum:i36DA08FFC583D250
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti39 – 391G → S in AAH31113 (PubMed:15489334).Curated
Sequence conflicti109 – 1091W → R in AAH31113 (PubMed:15489334).Curated
Sequence conflicti194 – 1941M → I in AAH31113 (PubMed:15489334).Curated
Sequence conflicti290 – 2901Q → QQ in AAH31113 (PubMed:15489334).Curated
Sequence conflicti1014 – 10141P → L in AAF35973 (PubMed:10681503).Curated
Sequence conflicti1141 – 11422SE → RS in AAF35973 (PubMed:10681503).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei458 – 20671610Missing in isoform 2. 2 PublicationsVSP_003410Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216186 mRNA. Translation: AAF35860.1.
BC031113 mRNA. Translation: AAH31113.1.
AF135169 mRNA. Translation: AAF35973.1.
UniGeneiMm.27592.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216186 mRNA. Translation: AAF35860.1.
BC031113 mRNA. Translation: AAH31113.1.
AF135169 mRNA. Translation: AAF35973.1.
UniGeneiMm.27592.

3D structure databases

ProteinModelPortaliQ9JL19.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61283N.
IntActiQ9JL19. 1 interaction.
MINTiMINT-5200950.
STRINGi10090.ENSMUSP00000045386.

PTM databases

iPTMnetiQ9JL19.
PhosphoSiteiQ9JL19.

Proteomic databases

EPDiQ9JL19.
MaxQBiQ9JL19.
PaxDbiQ9JL19.
PRIDEiQ9JL19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1929915. Ncoa6.

Phylogenomic databases

eggNOGiENOG410IK20. Eukaryota.
ENOG410XW4F. LUCA.
HOVERGENiHBG052586.
InParanoidiQ9JL19.

Miscellaneous databases

ChiTaRSiNcoa6. mouse.
PROiQ9JL19.
SOURCEiSearch...

Gene expression databases

CleanExiMM_NCOA6.

Family and domain databases

InterProiIPR026638. NCOA6.
IPR032715. NCOA6_nucleic_acid-bd.
[Graphical view]
PANTHERiPTHR15690:SF0. PTHR15690:SF0. 2 hits.
PfamiPF13820. Nucleic_acid_bd. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of peroxisome proliferator-activated receptor (PPAR) interacting protein (PRIP) as a coactivator for PPAR."
    Zhu Y.-J., Kan L., Qi C., Kanwar Y.S., Yeldandi A.V., Rao M.S., Reddy J.K.
    J. Biol. Chem. 275:13510-13516(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INTERACTION WITH PPARA; PPARG; RARA; RXRA; ESR1; ESR2 AND THRB.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "Cloning and characterization of RAP250, a nuclear receptor coactivator."
    Caira F., Antonson P., Pelto-Huikko M., Treuter E., Gustafsson J.-A.
    J. Biol. Chem. 275:5308-5317(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 786-1142 (ISOFORM 1), INTERACTION WITH PPARA; PPARG; ESR1; ESR2; THRA AND THRB, MUTAGENESIS OF 891-LEU--LEU-894.
    Tissue: Embryo.
  4. "Molecular cloning and characterization of CAPER, a novel coactivator of activating protein-1 and estrogen receptors."
    Jung D.-J., Na S.-Y., Na D.S., Lee J.W.
    J. Biol. Chem. 277:1229-1234(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RBM39.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2022, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung and Spleen.
  6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-1822 AND LYS-1825, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiNCOA6_MOUSE
AccessioniPrimary (citable) accession number: Q9JL19
Secondary accession number(s): Q9JLT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: October 1, 2000
Last modified: March 16, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.