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Protein

Beta-secretase 2

Gene

Bace2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672 (By similarity).By similarity

Catalytic activityi

Broad endopeptidase specificity. Cleaves Glu-Val-Asn-Leu-|-Asp-Ala-Glu-Phe in the Swedish variant of Alzheimer's amyloid precursor protein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei106PROSITE-ProRule annotation1
Active sitei299PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Protein family/group databases

MEROPSiA01.041.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-secretase 2 (EC:3.4.23.45)
Alternative name(s):
Aspartyl protease 1
Short name:
ASP1
Short name:
Asp 1
Beta-site amyloid precursor protein cleaving enzyme 2
Short name:
Beta-site APP cleaving enzyme 2
Memapsin-1
Membrane-associated aspartic protease 1
Theta-secretase
Gene namesi
Name:Bace2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1860440. Bace2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 469ExtracellularSequence analysisAdd BLAST450
Transmembranei470 – 490HelicalSequence analysisAdd BLAST21
Topological domaini491 – 514CytoplasmicSequence analysisAdd BLAST24

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3638357.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000038364620 – 62By similarityAdd BLAST43
ChainiPRO_000038364763 – 514Beta-secretase 2Add BLAST452

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi166N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi229 ↔ 429By similarity
Disulfide bondi288 ↔ 453By similarity
Disulfide bondi340 ↔ 389By similarity
Glycosylationi362N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Undergoes autoproteolytic cleavage.By similarity
Glycosylated.By similarity

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiQ9JL18.
PaxDbiQ9JL18.
PRIDEiQ9JL18.

PTM databases

iPTMnetiQ9JL18.
PhosphoSitePlusiQ9JL18.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040605.

Interactioni

Subunit structurei

Monomer. Interacts ith RTN3 and RTN4 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043918.

Chemistry databases

BindingDBiQ9JL18.

Structurei

3D structure databases

ProteinModelPortaliQ9JL18.
SMRiQ9JL18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini88 – 425Peptidase A1PROSITE-ProRule annotationAdd BLAST338

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
HOGENOMiHOG000036572.
InParanoidiQ9JL18.
KOiK07747.
PhylomeDBiQ9JL18.
TreeFamiTF329595.

Family and domain databases

CDDicd05473. beta_secretase_like. 1 hit.
Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR009119. BACE.
IPR009121. BACE2.
IPR033874. Memapsin-like.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PTHR13683:SF262. PTHR13683:SF262. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR01817. BACE2.
PR01815. BACEFAMILY.
PR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JL18-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGALLRALLL LVLAQWLLSA VPALAPAPFT LPLQVAGATN HRASAVPGLG
60 70 80 90 100
TPELPRADGL ALALEPVRAT ANFLAMVDNL QGDSGRGYYL EMLIGTPPQK
110 120 130 140 150
VQILVDTGSS NFAVAGAPHS YIDTYFDSES SSTYHSKGFD VTVKYTQGSW
160 170 180 190 200
TGFVGEDLVT IPKGFNSSFL VNIATIFESE NFFLPGIKWN GILGLAYAAL
210 220 230 240 250
AKPSSSLETF FDSLVAQAKI PDIFSMQMCG AGLPVAGSGT NGGSLVLGGI
260 270 280 290 300
EPSLYKGDIW YTPIKEEWYY QIEILKLEIG GQNLNLDCRE YNADKAIVDS
310 320 330 340 350
GTTLLRLPQK VFDAVVEAVA RTSLIPEFSD GFWTGAQLAC WTNSETPWAY
360 370 380 390 400
FPKISIYLRD ENASRSFRIT ILPQLYIQPM MGAGFNYECY RFGISSSTNA
410 420 430 440 450
LVIGATVMEG FYVVFDRAQR RVGFAVSPCA EIEGTTVSEI SGPFSTEDIA
460 470 480 490 500
SNCVPAQALN EPILWIVSYA LMSVCGAILL VLILLLLLPL HCRHAPRDPE
510
VVNDESSLVR HRWK
Length:514
Mass (Da):55,800
Last modified:October 1, 2000 - v1
Checksum:iA70725F2C1DF5B47
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11L → P in BAC34931 (PubMed:16141072).Curated1
Sequence conflicti11L → P in BAE38011 (PubMed:16141072).Curated1
Sequence conflicti11L → P in BAC37384 (PubMed:16141072).Curated1
Sequence conflicti37G → R in BAC34931 (PubMed:16141072).Curated1
Sequence conflicti37G → R in BAE38011 (PubMed:16141072).Curated1
Sequence conflicti37G → R in BAC37384 (PubMed:16141072).Curated1
Sequence conflicti53E → G in BAC37384 (PubMed:16141072).Curated1
Sequence conflicti369I → T in BAC34931 (PubMed:16141072).Curated1
Sequence conflicti488L → V in AAD45964 (PubMed:10683441).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216310 mRNA. Translation: AAF36599.1.
AK052309 mRNA. Translation: BAC34931.1.
AK078770 mRNA. Translation: BAC37384.1.
AK165036 mRNA. Translation: BAE38011.1.
CH466602 Genomic DNA. Translation: EDL03663.1.
BC120773 mRNA. Translation: AAI20774.1.
BC131947 mRNA. Translation: AAI31948.1.
AF051150 mRNA. Translation: AAD45964.1.
CCDSiCCDS28359.1.
RefSeqiNP_062390.3. NM_019517.5.
UniGeneiMm.102018.
Mm.97885.

Genome annotation databases

GeneIDi56175.
KEGGimmu:56175.
UCSCiuc008ade.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216310 mRNA. Translation: AAF36599.1.
AK052309 mRNA. Translation: BAC34931.1.
AK078770 mRNA. Translation: BAC37384.1.
AK165036 mRNA. Translation: BAE38011.1.
CH466602 Genomic DNA. Translation: EDL03663.1.
BC120773 mRNA. Translation: AAI20774.1.
BC131947 mRNA. Translation: AAI31948.1.
AF051150 mRNA. Translation: AAD45964.1.
CCDSiCCDS28359.1.
RefSeqiNP_062390.3. NM_019517.5.
UniGeneiMm.102018.
Mm.97885.

3D structure databases

ProteinModelPortaliQ9JL18.
SMRiQ9JL18.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043918.

Chemistry databases

BindingDBiQ9JL18.
ChEMBLiCHEMBL3638357.

Protein family/group databases

MEROPSiA01.041.

PTM databases

iPTMnetiQ9JL18.
PhosphoSitePlusiQ9JL18.

Proteomic databases

MaxQBiQ9JL18.
PaxDbiQ9JL18.
PRIDEiQ9JL18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi56175.
KEGGimmu:56175.
UCSCiuc008ade.2. mouse.

Organism-specific databases

CTDi25825.
MGIiMGI:1860440. Bace2.

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
HOGENOMiHOG000036572.
InParanoidiQ9JL18.
KOiK07747.
PhylomeDBiQ9JL18.
TreeFamiTF329595.

Miscellaneous databases

ChiTaRSiBace2. mouse.
PROiQ9JL18.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040605.

Family and domain databases

CDDicd05473. beta_secretase_like. 1 hit.
Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR009119. BACE.
IPR009121. BACE2.
IPR033874. Memapsin-like.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PTHR13683:SF262. PTHR13683:SF262. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR01817. BACE2.
PR01815. BACEFAMILY.
PR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBACE2_MOUSE
AccessioniPrimary (citable) accession number: Q9JL18
Secondary accession number(s): Q3TNS7
, Q8C5E9, Q8C793, Q9R1P7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.