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Q9JL18

- BACE2_MOUSE

UniProt

Q9JL18 - BACE2_MOUSE

Protein

Beta-secretase 2

Gene

Bace2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672 By similarity.By similarity

    Catalytic activityi

    Broad endopeptidase specificity. Cleaves Glu-Val-Asn-Leu-|-Asp-Ala-Glu-Phe in the Swedish variant of Alzheimer amyloid precursor protein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei106 – 1061PROSITE-ProRule annotation
    Active sitei299 – 2991PROSITE-ProRule annotation

    GO - Molecular functioni

    1. aspartic-type endopeptidase activity Source: UniProtKB

    GO - Biological processi

    1. membrane protein ectodomain proteolysis Source: UniProtKB
    2. negative regulation of amyloid precursor protein biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Aspartyl protease, Hydrolase, Protease

    Protein family/group databases

    MEROPSiA01.041.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-secretase 2 (EC:3.4.23.45)
    Alternative name(s):
    Aspartyl protease 1
    Short name:
    ASP1
    Short name:
    Asp 1
    Beta-site amyloid precursor protein cleaving enzyme 2
    Short name:
    Beta-site APP cleaving enzyme 2
    Memapsin-1
    Membrane-associated aspartic protease 1
    Theta-secretase
    Gene namesi
    Name:Bace2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:1860440. Bace2.

    Subcellular locationi

    Membrane By similarity; Single-pass type I membrane protein By similarity. Golgi apparatus By similarity. Endoplasmic reticulum By similarity. Endosome By similarity. Cell surface By similarity

    GO - Cellular componenti

    1. cell surface Source: UniProtKB-SubCell
    2. endoplasmic reticulum Source: UniProtKB-SubCell
    3. endosome Source: UniProtKB-SubCell
    4. Golgi apparatus Source: UniProtKB
    5. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Propeptidei20 – 6243By similarityPRO_0000383646Add
    BLAST
    Chaini63 – 514452Beta-secretase 2PRO_0000383647Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi229 ↔ 429By similarity
    Disulfide bondi288 ↔ 453By similarity
    Disulfide bondi340 ↔ 389By similarity
    Glycosylationi362 – 3621N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Undergoes autoproteolytic cleavage.By similarity
    Glycosylated.By similarity

    Keywords - PTMi

    Autocatalytic cleavage, Disulfide bond, Glycoprotein, Zymogen

    Expressioni

    Gene expression databases

    BgeeiQ9JL18.
    GenevestigatoriQ9JL18.

    Interactioni

    Subunit structurei

    Monomer. Interacts ith RTN3 and RTN4 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JL18.
    SMRiQ9JL18. Positions 72-455.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini20 – 469450ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini491 – 51424CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei470 – 49021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase A1 family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG267436.
    GeneTreeiENSGT00710000106265.
    HOGENOMiHOG000036572.
    InParanoidiQ8C793.
    KOiK07747.
    OrthoDBiEOG7DNNVW.
    PhylomeDBiQ9JL18.
    TreeFamiTF329595.

    Family and domain databases

    Gene3Di2.40.70.10. 2 hits.
    InterProiIPR001461. Aspartic_peptidase.
    IPR001969. Aspartic_peptidase_AS.
    IPR009119. Pept_A1_BACE.
    IPR009121. Pept_A1_BACE2.
    IPR021109. Peptidase_aspartic_dom.
    [Graphical view]
    PANTHERiPTHR13683. PTHR13683. 1 hit.
    PfamiPF00026. Asp. 1 hit.
    [Graphical view]
    PRINTSiPR01817. BACE2.
    PR01815. BACEFAMILY.
    PR00792. PEPSIN.
    SUPFAMiSSF50630. SSF50630. 1 hit.
    PROSITEiPS00141. ASP_PROTEASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9JL18-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGALLRALLL LVLAQWLLSA VPALAPAPFT LPLQVAGATN HRASAVPGLG    50
    TPELPRADGL ALALEPVRAT ANFLAMVDNL QGDSGRGYYL EMLIGTPPQK 100
    VQILVDTGSS NFAVAGAPHS YIDTYFDSES SSTYHSKGFD VTVKYTQGSW 150
    TGFVGEDLVT IPKGFNSSFL VNIATIFESE NFFLPGIKWN GILGLAYAAL 200
    AKPSSSLETF FDSLVAQAKI PDIFSMQMCG AGLPVAGSGT NGGSLVLGGI 250
    EPSLYKGDIW YTPIKEEWYY QIEILKLEIG GQNLNLDCRE YNADKAIVDS 300
    GTTLLRLPQK VFDAVVEAVA RTSLIPEFSD GFWTGAQLAC WTNSETPWAY 350
    FPKISIYLRD ENASRSFRIT ILPQLYIQPM MGAGFNYECY RFGISSSTNA 400
    LVIGATVMEG FYVVFDRAQR RVGFAVSPCA EIEGTTVSEI SGPFSTEDIA 450
    SNCVPAQALN EPILWIVSYA LMSVCGAILL VLILLLLLPL HCRHAPRDPE 500
    VVNDESSLVR HRWK 514
    Length:514
    Mass (Da):55,800
    Last modified:October 1, 2000 - v1
    Checksum:iA70725F2C1DF5B47
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti11 – 111L → P in BAC34931. (PubMed:16141072)Curated
    Sequence conflicti11 – 111L → P in BAE38011. (PubMed:16141072)Curated
    Sequence conflicti11 – 111L → P in BAC37384. (PubMed:16141072)Curated
    Sequence conflicti37 – 371G → R in BAC34931. (PubMed:16141072)Curated
    Sequence conflicti37 – 371G → R in BAE38011. (PubMed:16141072)Curated
    Sequence conflicti37 – 371G → R in BAC37384. (PubMed:16141072)Curated
    Sequence conflicti53 – 531E → G in BAC37384. (PubMed:16141072)Curated
    Sequence conflicti369 – 3691I → T in BAC34931. (PubMed:16141072)Curated
    Sequence conflicti488 – 4881L → V in AAD45964. (PubMed:10683441)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF216310 mRNA. Translation: AAF36599.1.
    AK052309 mRNA. Translation: BAC34931.1.
    AK078770 mRNA. Translation: BAC37384.1.
    AK165036 mRNA. Translation: BAE38011.1.
    CH466602 Genomic DNA. Translation: EDL03663.1.
    BC120773 mRNA. Translation: AAI20774.1.
    BC131947 mRNA. Translation: AAI31948.1.
    AF051150 mRNA. Translation: AAD45964.1.
    CCDSiCCDS28359.1.
    RefSeqiNP_062390.3. NM_019517.4.
    UniGeneiMm.97885.

    Genome annotation databases

    EnsembliENSMUST00000047275; ENSMUSP00000043918; ENSMUSG00000040605.
    GeneIDi56175.
    KEGGimmu:56175.
    UCSCiuc008ade.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF216310 mRNA. Translation: AAF36599.1 .
    AK052309 mRNA. Translation: BAC34931.1 .
    AK078770 mRNA. Translation: BAC37384.1 .
    AK165036 mRNA. Translation: BAE38011.1 .
    CH466602 Genomic DNA. Translation: EDL03663.1 .
    BC120773 mRNA. Translation: AAI20774.1 .
    BC131947 mRNA. Translation: AAI31948.1 .
    AF051150 mRNA. Translation: AAD45964.1 .
    CCDSi CCDS28359.1.
    RefSeqi NP_062390.3. NM_019517.4.
    UniGenei Mm.97885.

    3D structure databases

    ProteinModelPortali Q9JL18.
    SMRi Q9JL18. Positions 72-455.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi A01.041.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000047275 ; ENSMUSP00000043918 ; ENSMUSG00000040605 .
    GeneIDi 56175.
    KEGGi mmu:56175.
    UCSCi uc008ade.2. mouse.

    Organism-specific databases

    CTDi 25825.
    MGIi MGI:1860440. Bace2.

    Phylogenomic databases

    eggNOGi NOG267436.
    GeneTreei ENSGT00710000106265.
    HOGENOMi HOG000036572.
    InParanoidi Q8C793.
    KOi K07747.
    OrthoDBi EOG7DNNVW.
    PhylomeDBi Q9JL18.
    TreeFami TF329595.

    Miscellaneous databases

    ChiTaRSi BACE2. mouse.
    NextBioi 311944.
    PROi Q9JL18.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9JL18.
    Genevestigatori Q9JL18.

    Family and domain databases

    Gene3Di 2.40.70.10. 2 hits.
    InterProi IPR001461. Aspartic_peptidase.
    IPR001969. Aspartic_peptidase_AS.
    IPR009119. Pept_A1_BACE.
    IPR009121. Pept_A1_BACE2.
    IPR021109. Peptidase_aspartic_dom.
    [Graphical view ]
    PANTHERi PTHR13683. PTHR13683. 1 hit.
    Pfami PF00026. Asp. 1 hit.
    [Graphical view ]
    PRINTSi PR01817. BACE2.
    PR01815. BACEFAMILY.
    PR00792. PEPSIN.
    SUPFAMi SSF50630. SSF50630. 1 hit.
    PROSITEi PS00141. ASP_PROTEASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of the mouse Asp1 gene, a homolog of the human ASP1 (Down Syndrome region aspartyl protease)."
      Choi D.K., Sugano S., Sakaki Y.
      Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Eye, Heart and Testis.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    5. "The gene encoding DRAP (BACE2), a glycosylated transmembrane protein of the aspartic protease family, maps to the down critical region."
      Acquati F., Accarino M.P., Nucci C., Fumagalli P., Jovine L., Ottolenghi S., Taramelli R.
      FEBS Lett. 468:59-64(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 260-514.

    Entry informationi

    Entry nameiBACE2_MOUSE
    AccessioniPrimary (citable) accession number: Q9JL18
    Secondary accession number(s): Q3TNS7
    , Q8C5E9, Q8C793, Q9R1P7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3