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Protein

Galectin-8

Gene

Lgals8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy. Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane; these ligands becoming exposed to the cytoplasm following rupture. Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52. Required to restrict infection of bacterial invasion such as S.typhimurium. Also required to restrict infection of Picornaviridae viruses. Has a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei58Critical for binding to sialylated and sulfated oligosaccharidesBy similarity1
Binding sitei68CarbohydrateBy similarity1
Binding sitei78CarbohydrateBy similarity1
Binding sitei88CarbohydrateBy similarity1

GO - Molecular functioni

  • carbohydrate binding Source: MGI

GO - Biological processi

  • cellular response to virus Source: UniProtKB
  • plasma cell differentiation Source: MGI
  • T cell costimulation Source: MGI
  • xenophagy Source: GO_Central

Keywordsi

Biological processAutophagy
LigandLectin

Names & Taxonomyi

Protein namesi
Recommended name:
Galectin-8
Short name:
Gal-8
Alternative name(s):
LGALS-8
Gene namesi
Name:Lgals8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1928481. Lgals8.

Subcellular locationi

  • Cytoplasmic vesicle By similarity
  • Cytoplasmcytosol By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000769441 – 316Galectin-8Add BLAST316

Proteomic databases

MaxQBiQ9JL15.
PaxDbiQ9JL15.
PRIDEiQ9JL15.

PTM databases

iPTMnetiQ9JL15.
PhosphoSitePlusiQ9JL15.

Expressioni

Gene expression databases

BgeeiENSMUSG00000057554.
CleanExiMM_LGALS8.
ExpressionAtlasiQ9JL15. baseline and differential.
GenevisibleiQ9JL15. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with CALCOCO2/NDP52.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000097408.

Structurei

3D structure databases

ProteinModelPortaliQ9JL15.
SMRiQ9JL15.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 151Galectin 1PROSITE-ProRule annotationAdd BLAST134
Domaini186 – 316Galectin 2PROSITE-ProRule annotationAdd BLAST131

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni248 – 254Beta-galactoside bindingBy similarity7

Domaini

Contains two homologous but distinct carbohydrate-binding domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3587. Eukaryota.
ENOG4111EA0. LUCA.
GeneTreeiENSGT00760000119105.
HOGENOMiHOG000290194.
HOVERGENiHBG002412.
InParanoidiQ9JL15.
KOiK06832.
PhylomeDBiQ9JL15.
TreeFamiTF315551.

Family and domain databases

InterProiView protein in InterPro
IPR013320. ConA-like_dom.
IPR030638. Galectin_8.
IPR001079. Galectin_CRD.
PANTHERiPTHR11346:SF125. PTHR11346:SF125. 1 hit.
PfamiView protein in Pfam
PF00337. Gal-bind_lectin. 2 hits.
SMARTiView protein in SMART
SM00908. Gal-bind_lectin. 2 hits.
SM00276. GLECT. 2 hits.
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiView protein in PROSITE
PS51304. GALECTIN. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9JL15-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSLNNLQNI IYNPIIPYVG TITEQLKPGS LIVIRGHVPK DSERFQVDFQ
60 70 80 90 100
LGNSLKPRAD VAFHFNPRFK RSSCIVCNTL TQEKWGWEEI TYDMPFRKEK
110 120 130 140 150
SFEIVFMVLK NKFQVAVNGR HVLLYAHRIS PEQIDTVGIY GKVNIHSIGF
160 170 180 190 200
RFSSDLQSME TSALGLTQIN RENIQKPGKL QLSLPFEARL NASMGPGRTV
210 220 230 240 250
VIKGEVNTNA RSFNVDLVAG KTRDIALHLN PRLNVKAFVR NSFLQDAWGE
260 270 280 290 300
EERNITCFPF SSGMYFEMII YCDVREFKVA INGVHSLEYK HRFKDLSSID
310
TLSVDGDIRL LDVRSW
Length:316
Mass (Da):36,162
Last modified:October 1, 2000 - v1
Checksum:i11A20309AEF52C69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218069 mRNA. Translation: AAF27645.1.
AK029526 mRNA. Translation: BAC26495.1.
BC040243 mRNA. Translation: AAH40243.1.
CCDSiCCDS36592.1.
RefSeqiNP_001185972.1. NM_001199043.1.
NP_061374.1. NM_018886.5.
XP_006516777.1. XM_006516714.2.
XP_006516778.1. XM_006516715.2.
UniGeneiMm.474138.
Mm.486765.

Genome annotation databases

EnsembliENSMUST00000099820; ENSMUSP00000097408; ENSMUSG00000057554.
ENSMUST00000099821; ENSMUSP00000097409; ENSMUSG00000057554.
ENSMUST00000124888; ENSMUSP00000115094; ENSMUSG00000057554.
GeneIDi56048.
KEGGimmu:56048.
UCSCiuc007plm.3. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Galectin-8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218069 mRNA. Translation: AAF27645.1.
AK029526 mRNA. Translation: BAC26495.1.
BC040243 mRNA. Translation: AAH40243.1.
CCDSiCCDS36592.1.
RefSeqiNP_001185972.1. NM_001199043.1.
NP_061374.1. NM_018886.5.
XP_006516777.1. XM_006516714.2.
XP_006516778.1. XM_006516715.2.
UniGeneiMm.474138.
Mm.486765.

3D structure databases

ProteinModelPortaliQ9JL15.
SMRiQ9JL15.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000097408.

PTM databases

iPTMnetiQ9JL15.
PhosphoSitePlusiQ9JL15.

Proteomic databases

MaxQBiQ9JL15.
PaxDbiQ9JL15.
PRIDEiQ9JL15.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000099820; ENSMUSP00000097408; ENSMUSG00000057554.
ENSMUST00000099821; ENSMUSP00000097409; ENSMUSG00000057554.
ENSMUST00000124888; ENSMUSP00000115094; ENSMUSG00000057554.
GeneIDi56048.
KEGGimmu:56048.
UCSCiuc007plm.3. mouse.

Organism-specific databases

CTDi3964.
MGIiMGI:1928481. Lgals8.

Phylogenomic databases

eggNOGiKOG3587. Eukaryota.
ENOG4111EA0. LUCA.
GeneTreeiENSGT00760000119105.
HOGENOMiHOG000290194.
HOVERGENiHBG002412.
InParanoidiQ9JL15.
KOiK06832.
PhylomeDBiQ9JL15.
TreeFamiTF315551.

Miscellaneous databases

ChiTaRSiLgals8. mouse.
PROiPR:Q9JL15.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000057554.
CleanExiMM_LGALS8.
ExpressionAtlasiQ9JL15. baseline and differential.
GenevisibleiQ9JL15. MM.

Family and domain databases

InterProiView protein in InterPro
IPR013320. ConA-like_dom.
IPR030638. Galectin_8.
IPR001079. Galectin_CRD.
PANTHERiPTHR11346:SF125. PTHR11346:SF125. 1 hit.
PfamiView protein in Pfam
PF00337. Gal-bind_lectin. 2 hits.
SMARTiView protein in SMART
SM00908. Gal-bind_lectin. 2 hits.
SM00276. GLECT. 2 hits.
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiView protein in PROSITE
PS51304. GALECTIN. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiLEG8_MOUSE
AccessioniPrimary (citable) accession number: Q9JL15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: October 1, 2000
Last modified: May 10, 2017
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.