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Q9JKZ2 (SC5A3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium/myo-inositol cotransporter

Short name=Na(+)/myo-inositol cotransporter
Alternative name(s):
Sodium/myo-inositol transporter 1
Short name=SMIT1
Solute carrier family 5 member 3
Gene names
Name:Slc5a3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length718 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Prevents intracellular accumulation of high concentrations of myo-inositol (an osmolyte) that result in impairment of cellular function. Ref.4

Functions as a retroviral receptor for M813 murine leukemia virus (MuLV) entry. Ref.4

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 718718Sodium/myo-inositol cotransporter
PRO_0000105382

Regions

Topological domain1 – 99Extracellular Potential
Transmembrane10 – 2920Helical; Potential
Topological domain30 – 389Cytoplasmic Potential
Transmembrane39 – 5719Helical; Potential
Topological domain58 – 8629Extracellular Potential
Transmembrane87 – 11024Helical; Potential
Topological domain111 – 12313Cytoplasmic Potential
Transmembrane124 – 14421Helical; Potential
Topological domain145 – 15713Extracellular Potential
Transmembrane158 – 18326Helical; Potential
Topological domain184 – 1863Cytoplasmic Potential
Transmembrane187 – 20519Helical; Potential
Topological domain206 – 30398Extracellular Potential
Transmembrane304 – 32421Helical; Potential
Topological domain325 – 35329Cytoplasmic Potential
Transmembrane354 – 37623Helical; Potential
Topological domain377 – 40630Extracellular Potential
Transmembrane407 – 43024Helical; Potential
Topological domain431 – 44313Cytoplasmic Potential
Transmembrane444 – 46219Helical; Potential
Topological domain463 – 51048Extracellular Potential
Transmembrane511 – 53222Helical; Potential
Topological domain533 – 695163Cytoplasmic Potential
Transmembrane696 – 71621Helical; Potential
Topological domain717 – 7182Extracellular Potential

Sites

Site241Implicated in sodium coupling By similarity
Site2851Implicated in sodium coupling By similarity

Amino acid modifications

Glycosylation2321N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict6531R → Q in AAF43668. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9JKZ2 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: D0371D6ECF08503B

FASTA71879,583
        10         20         30         40         50         60 
MRAVLEAADI AVVALYFILV MCIGFFAMWK SNRSTVSGYF LAGRSMTWVA IGASLFVSNI 

        70         80         90        100        110        120 
GSEHFIGLAG SGAASGFAVG AWEFNALLLL QLLGWVFIPI YIRSGVYTMP EYLSKRFGGH 

       130        140        150        160        170        180 
RIQVYFAALS LLLYIFTKLS VDLYSGALFI QESLGWNLYV SVILLIGMTA LLTVTGGLVA 

       190        200        210        220        230        240 
VIYTDTLQAL LMIIGALTLM VISMVKIGGF EEVKRRYMLA SPDVASILLK YNLSNTNACM 

       250        260        270        280        290        300 
VHPKANALKM LRDPTDEDVP WPGFILGQTP ASVWYWCADQ VIVQRVLAAK NIAHAKGSTL 

       310        320        330        340        350        360 
MAGFLKLLPM FIIVVPGMIS RIVFADEIAC INPEHCMQVC GSRAGCSNIA YPRLVMTLVP 

       370        380        390        400        410        420 
VGLRGLMMAV MIAALMSDLD SIFNSASTIF TLDVYKLIRK SASSRELMIV GRIFVAFMVV 

       430        440        450        460        470        480 
ISIAWVPIIV EMQGGQMYLY IQEVADYLTP PVAALFLLAI FWKRCNEQGA FYGGMAGFVL 

       490        500        510        520        530        540 
GAVRLILAFT YRAPECDQPD NRPGFIKDIH YMYVATALFW ITGLITVIVS LLTPPPTKDQ 

       550        560        570        580        590        600 
IRTTTFWSKK TLVTKESCSQ KDEPYKMQEK SILQCSENSE VISHTIPNGK SEDSIKGLQP 

       610        620        630        640        650        660 
EDVNLLVTCR EEGNPVASMG HSEAETPVDA YSNGQAALMG EREREKETEN RSRYWKFIDW 

       670        680        690        700        710 
FCGFKSKSLS KRSLRDLMDE EAVCLQMLEE TPQVKVILNI GLFAVCSLGI FMFVYFSL 

« Hide

References

« Hide 'large scale' references
[1]"Murine chromosome 16 telomeric region, homologous with human chromosome 21q22, contains the osmoregulatory Na(+)/myo-inositol cotransporter (SLC5A3) gene."
McVeigh K.E., Mallee J.J., Lucente A., Barnoski B.L., Wu S., Berry G.T.
Cytogenet. Cell Genet. 88:153-158(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"Sodium-dependent myo-inositol transporter 1 is a cellular receptor for Mus cervicolor M813 murine leukemia virus."
Hein S., Prassolov V., Zhang Y., Ivanov D., Lohler J., Ross S.R., Stocking C.
J. Virol. 77:5926-5932(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS M813 MURINE LEUKEMIA VIRUS RECEPTOR, TOPOLOGY.
[5]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF220915 Genomic DNA. Translation: AAF43668.1.
CH466602 Genomic DNA. Translation: EDL03793.1.
BC140982 mRNA. Translation: AAI40983.1.
BC140983 mRNA. Translation: AAI40984.1.
RefSeqNP_059087.2. NM_017391.3.
UniGeneMm.217354.
Mm.451146.

3D structure databases

ProteinModelPortalQ9JKZ2.
SMRQ9JKZ2. Positions 6-538.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ9JKZ2.

Proteomic databases

PaxDbQ9JKZ2.
PRIDEQ9JKZ2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000113975; ENSMUSP00000109608; ENSMUSG00000089774.
GeneID53881.
KEGGmmu:53881.
UCSCuc007zyt.2. mouse.

Organism-specific databases

CTD6526.
MGIMGI:1858226. Slc5a3.

Phylogenomic databases

eggNOGCOG4146.
GeneTreeENSGT00550000074313.
HOGENOMHOG000025422.
HOVERGENHBG052859.
InParanoidB2RU60.
KOK14383.
OMASRYWKFI.
OrthoDBEOG77126J.
TreeFamTF352855.

Gene expression databases

BgeeQ9JKZ2.
GenevestigatorQ9JKZ2.

Family and domain databases

InterProIPR001734. Na/solute_symporter.
IPR018212. Na/solute_symporter_CS.
IPR019900. Na/solute_symporter_subgr.
[Graphical view]
PANTHERPTHR11819. PTHR11819. 1 hit.
PfamPF00474. SSF. 1 hit.
[Graphical view]
TIGRFAMsTIGR00813. sss. 1 hit.
PROSITEPS00456. NA_SOLUT_SYMP_1. 1 hit.
PS00457. NA_SOLUT_SYMP_2. 1 hit.
PS50283. NA_SOLUT_SYMP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio310757.
PROQ9JKZ2.
SOURCESearch...

Entry information

Entry nameSC5A3_MOUSE
AccessionPrimary (citable) accession number: Q9JKZ2
Secondary accession number(s): B2RU60
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot