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Protein

Sodium/myo-inositol cotransporter

Gene

Slc5a3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Prevents intracellular accumulation of high concentrations of myo-inositol (an osmolyte) that result in impairment of cellular function.1 Publication
Functions as a retroviral receptor for M813 murine leukemia virus (MuLV) entry.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei24 – 241Implicated in sodium couplingBy similarity
Sitei285 – 2851Implicated in sodium couplingBy similarity

GO - Molecular functioni

GO - Biological processi

  • inositol metabolic process Source: MGI
  • myo-inositol transport Source: MGI
  • peripheral nervous system development Source: MGI
  • regulation of respiratory gaseous exchange Source: MGI
  • sodium ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiREACT_294731. Inositol transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/myo-inositol cotransporter
Short name:
Na(+)/myo-inositol cotransporter
Alternative name(s):
Sodium/myo-inositol transporter 1
Short name:
SMIT1
Solute carrier family 5 member 3
Gene namesi
Name:Slc5a3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1858226. Slc5a3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99ExtracellularSequence Analysis
Transmembranei10 – 2920HelicalSequence AnalysisAdd
BLAST
Topological domaini30 – 389CytoplasmicSequence Analysis
Transmembranei39 – 5719HelicalSequence AnalysisAdd
BLAST
Topological domaini58 – 8629ExtracellularSequence AnalysisAdd
BLAST
Transmembranei87 – 11024HelicalSequence AnalysisAdd
BLAST
Topological domaini111 – 12313CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei124 – 14421HelicalSequence AnalysisAdd
BLAST
Topological domaini145 – 15713ExtracellularSequence AnalysisAdd
BLAST
Transmembranei158 – 18326HelicalSequence AnalysisAdd
BLAST
Topological domaini184 – 1863CytoplasmicSequence Analysis
Transmembranei187 – 20519HelicalSequence AnalysisAdd
BLAST
Topological domaini206 – 30398ExtracellularSequence AnalysisAdd
BLAST
Transmembranei304 – 32421HelicalSequence AnalysisAdd
BLAST
Topological domaini325 – 35329CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei354 – 37623HelicalSequence AnalysisAdd
BLAST
Topological domaini377 – 40630ExtracellularSequence AnalysisAdd
BLAST
Transmembranei407 – 43024HelicalSequence AnalysisAdd
BLAST
Topological domaini431 – 44313CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei444 – 46219HelicalSequence AnalysisAdd
BLAST
Topological domaini463 – 51048ExtracellularSequence AnalysisAdd
BLAST
Transmembranei511 – 53222HelicalSequence AnalysisAdd
BLAST
Topological domaini533 – 695163CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei696 – 71621HelicalSequence AnalysisAdd
BLAST
Topological domaini717 – 7182ExtracellularSequence Analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 718718Sodium/myo-inositol cotransporterPRO_0000105382Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi232 – 2321N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9JKZ2.
PaxDbiQ9JKZ2.
PRIDEiQ9JKZ2.

PTM databases

PhosphoSiteiQ9JKZ2.

Expressioni

Gene expression databases

BgeeiQ9JKZ2.
ExpressionAtlasiQ9JKZ2. baseline and differential.
GenevisibleiQ9JKZ2. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000109608.

Structurei

3D structure databases

ProteinModelPortaliQ9JKZ2.
SMRiQ9JKZ2. Positions 9-476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4146.
GeneTreeiENSGT00760000118955.
HOGENOMiHOG000025422.
HOVERGENiHBG052859.
InParanoidiQ9JKZ2.
KOiK14383.
OMAiTINHIIP.
OrthoDBiEOG77126J.
TreeFamiTF352855.

Family and domain databases

InterProiIPR001734. Na/solute_symporter.
IPR018212. Na/solute_symporter_CS.
IPR019900. Na/solute_symporter_subgr.
[Graphical view]
PANTHERiPTHR11819. PTHR11819. 1 hit.
PfamiPF00474. SSF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00813. sss. 1 hit.
PROSITEiPS00456. NA_SOLUT_SYMP_1. 1 hit.
PS00457. NA_SOLUT_SYMP_2. 1 hit.
PS50283. NA_SOLUT_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JKZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAVLEAADI AVVALYFILV MCIGFFAMWK SNRSTVSGYF LAGRSMTWVA
60 70 80 90 100
IGASLFVSNI GSEHFIGLAG SGAASGFAVG AWEFNALLLL QLLGWVFIPI
110 120 130 140 150
YIRSGVYTMP EYLSKRFGGH RIQVYFAALS LLLYIFTKLS VDLYSGALFI
160 170 180 190 200
QESLGWNLYV SVILLIGMTA LLTVTGGLVA VIYTDTLQAL LMIIGALTLM
210 220 230 240 250
VISMVKIGGF EEVKRRYMLA SPDVASILLK YNLSNTNACM VHPKANALKM
260 270 280 290 300
LRDPTDEDVP WPGFILGQTP ASVWYWCADQ VIVQRVLAAK NIAHAKGSTL
310 320 330 340 350
MAGFLKLLPM FIIVVPGMIS RIVFADEIAC INPEHCMQVC GSRAGCSNIA
360 370 380 390 400
YPRLVMTLVP VGLRGLMMAV MIAALMSDLD SIFNSASTIF TLDVYKLIRK
410 420 430 440 450
SASSRELMIV GRIFVAFMVV ISIAWVPIIV EMQGGQMYLY IQEVADYLTP
460 470 480 490 500
PVAALFLLAI FWKRCNEQGA FYGGMAGFVL GAVRLILAFT YRAPECDQPD
510 520 530 540 550
NRPGFIKDIH YMYVATALFW ITGLITVIVS LLTPPPTKDQ IRTTTFWSKK
560 570 580 590 600
TLVTKESCSQ KDEPYKMQEK SILQCSENSE VISHTIPNGK SEDSIKGLQP
610 620 630 640 650
EDVNLLVTCR EEGNPVASMG HSEAETPVDA YSNGQAALMG EREREKETEN
660 670 680 690 700
RSRYWKFIDW FCGFKSKSLS KRSLRDLMDE EAVCLQMLEE TPQVKVILNI
710
GLFAVCSLGI FMFVYFSL
Length:718
Mass (Da):79,583
Last modified:July 27, 2011 - v2
Checksum:iD0371D6ECF08503B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti653 – 6531R → Q in AAF43668 (PubMed:10773690).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF220915 Genomic DNA. Translation: AAF43668.1.
CH466602 Genomic DNA. Translation: EDL03793.1.
BC140982 mRNA. Translation: AAI40983.1.
BC140983 mRNA. Translation: AAI40984.1.
CCDSiCCDS37403.1.
RefSeqiNP_059087.2. NM_017391.3.
UniGeneiMm.217354.
Mm.451146.

Genome annotation databases

EnsembliENSMUST00000113975; ENSMUSP00000109608; ENSMUSG00000089774.
GeneIDi53881.
KEGGimmu:53881.
UCSCiuc007zyt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF220915 Genomic DNA. Translation: AAF43668.1.
CH466602 Genomic DNA. Translation: EDL03793.1.
BC140982 mRNA. Translation: AAI40983.1.
BC140983 mRNA. Translation: AAI40984.1.
CCDSiCCDS37403.1.
RefSeqiNP_059087.2. NM_017391.3.
UniGeneiMm.217354.
Mm.451146.

3D structure databases

ProteinModelPortaliQ9JKZ2.
SMRiQ9JKZ2. Positions 9-476.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000109608.

PTM databases

PhosphoSiteiQ9JKZ2.

Proteomic databases

MaxQBiQ9JKZ2.
PaxDbiQ9JKZ2.
PRIDEiQ9JKZ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113975; ENSMUSP00000109608; ENSMUSG00000089774.
GeneIDi53881.
KEGGimmu:53881.
UCSCiuc007zyt.2. mouse.

Organism-specific databases

CTDi6526.
MGIiMGI:1858226. Slc5a3.

Phylogenomic databases

eggNOGiCOG4146.
GeneTreeiENSGT00760000118955.
HOGENOMiHOG000025422.
HOVERGENiHBG052859.
InParanoidiQ9JKZ2.
KOiK14383.
OMAiTINHIIP.
OrthoDBiEOG77126J.
TreeFamiTF352855.

Enzyme and pathway databases

ReactomeiREACT_294731. Inositol transporters.

Miscellaneous databases

NextBioi310757.
PROiQ9JKZ2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JKZ2.
ExpressionAtlasiQ9JKZ2. baseline and differential.
GenevisibleiQ9JKZ2. MM.

Family and domain databases

InterProiIPR001734. Na/solute_symporter.
IPR018212. Na/solute_symporter_CS.
IPR019900. Na/solute_symporter_subgr.
[Graphical view]
PANTHERiPTHR11819. PTHR11819. 1 hit.
PfamiPF00474. SSF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00813. sss. 1 hit.
PROSITEiPS00456. NA_SOLUT_SYMP_1. 1 hit.
PS00457. NA_SOLUT_SYMP_2. 1 hit.
PS50283. NA_SOLUT_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Murine chromosome 16 telomeric region, homologous with human chromosome 21q22, contains the osmoregulatory Na(+)/myo-inositol cotransporter (SLC5A3) gene."
    McVeigh K.E., Mallee J.J., Lucente A., Barnoski B.L., Wu S., Berry G.T.
    Cytogenet. Cell Genet. 88:153-158(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Sodium-dependent myo-inositol transporter 1 is a cellular receptor for Mus cervicolor M813 murine leukemia virus."
    Hein S., Prassolov V., Zhang Y., Ivanov D., Lohler J., Ross S.R., Stocking C.
    J. Virol. 77:5926-5932(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS M813 MURINE LEUKEMIA VIRUS RECEPTOR, TOPOLOGY.
  5. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSC5A3_MOUSE
AccessioniPrimary (citable) accession number: Q9JKZ2
Secondary accession number(s): B2RU60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: July 27, 2011
Last modified: July 22, 2015
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.