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Protein

Huntingtin-interacting protein 1-related protein

Gene

Hip1r

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.

GO - Molecular functioni

  • actin binding Source: MGI
  • actin filament binding Source: ParkinsonsUK-UCL
  • clathrin adaptor activity Source: ParkinsonsUK-UCL
  • clathrin binding Source: ParkinsonsUK-UCL
  • clathrin light chain binding Source: ParkinsonsUK-UCL
  • phosphatidylinositol-3,4,5-trisphosphate binding Source: MGI
  • phosphatidylinositol-3,4-bisphosphate binding Source: MGI
  • phosphatidylinositol-3,5-bisphosphate binding Source: MGI
  • phosphatidylinositol-4,5-bisphosphate binding Source: MGI
  • phosphatidylinositol binding Source: UniProtKB
  • protein heterodimerization activity Source: ParkinsonsUK-UCL
  • protein homodimerization activity Source: ParkinsonsUK-UCL
  • SH3 domain binding Source: ParkinsonsUK-UCL

GO - Biological processi

  • activation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • apoptotic process Source: InterPro
  • clathrin coat assembly Source: ParkinsonsUK-UCL
  • clathrin-dependent endocytosis Source: InterPro
  • digestive system development Source: ParkinsonsUK-UCL
  • membrane organization Source: ParkinsonsUK-UCL
  • negative regulation of actin filament polymerization Source: ParkinsonsUK-UCL
  • negative regulation of apoptotic process Source: ParkinsonsUK-UCL
  • negative regulation of Arp2/3 complex-mediated actin nucleation Source: ParkinsonsUK-UCL
  • positive regulation of apoptotic process Source: MGI
  • positive regulation of clathrin coat assembly Source: ParkinsonsUK-UCL
  • positive regulation of clathrin-dependent endocytosis Source: ParkinsonsUK-UCL
  • positive regulation of epidermal growth factor receptor signaling pathway Source: MGI
  • positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway Source: MGI
  • positive regulation of platelet-derived growth factor receptor-beta signaling pathway Source: MGI
  • positive regulation of protein binding Source: MGI
  • protein stabilization Source: MGI
  • receptor-mediated endocytosis Source: UniProtKB
  • regulation of actin cytoskeleton organization Source: ParkinsonsUK-UCL
  • regulation of clathrin-dependent endocytosis Source: ParkinsonsUK-UCL
  • regulation of endocytosis Source: MGI
  • regulation of gastric acid secretion Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiR-MMU-432722. Golgi Associated Vesicle Biogenesis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Huntingtin-interacting protein 1-related protein
Short name:
HIP1-related protein
Gene namesi
Name:Hip1r
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1352504. Hip1r.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: ParkinsonsUK-UCL
  • cell cortex Source: ParkinsonsUK-UCL
  • clathrin-coated pit Source: ParkinsonsUK-UCL
  • clathrin-coated vesicle Source: UniProtKB
  • clathrin-coated vesicle membrane Source: UniProtKB-SubCell
  • cytoplasm Source: MGI
  • cytoskeleton Source: MGI
  • dendrite cytoplasm Source: ParkinsonsUK-UCL
  • dendritic spine Source: ParkinsonsUK-UCL
  • intracellular membrane-bounded organelle Source: MGI
  • neuronal cell body Source: ParkinsonsUK-UCL
  • perinuclear region of cytoplasm Source: ParkinsonsUK-UCL
  • postsynaptic density Source: ParkinsonsUK-UCL
  • ruffle membrane Source: MGI
  • synaptic membrane Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Disruption phenotypei

Hip1 and Hip1r double knockout mice are dwarfed, afflicted with severe vertebral defects and die in early adulthood.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000839851 – 1068Huntingtin-interacting protein 1-related proteinAdd BLAST1068

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9JKY5.
MaxQBiQ9JKY5.
PaxDbiQ9JKY5.
PRIDEiQ9JKY5.

PTM databases

iPTMnetiQ9JKY5.
PhosphoSitePlusiQ9JKY5.

Expressioni

Tissue specificityi

Widely expressed. Expressed at lower levels in skeletal muscle and heart. The level of expression does not change appreciably during development.

Gene expression databases

BgeeiENSMUSG00000000915.
CleanExiMM_HIP1R.
ExpressionAtlasiQ9JKY5. baseline and differential.
GenevisibleiQ9JKY5. MM.

Interactioni

Subunit structurei

Interacts with actin. Does not interact with huntingtin (By similarity). Interacts with CLTB and HIP1. Homodimer. Homodimerization promotes actin binding.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CttnQ605984EBI-642457,EBI-397955
SH3KBP1Q96B973EBI-642457,EBI-346595From a different organism.

GO - Molecular functioni

  • actin binding Source: MGI
  • actin filament binding Source: ParkinsonsUK-UCL
  • clathrin adaptor activity Source: ParkinsonsUK-UCL
  • clathrin binding Source: ParkinsonsUK-UCL
  • clathrin light chain binding Source: ParkinsonsUK-UCL
  • protein heterodimerization activity Source: ParkinsonsUK-UCL
  • protein homodimerization activity Source: ParkinsonsUK-UCL
  • SH3 domain binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi205896. 1 interactor.
IntActiQ9JKY5. 8 interactors.
MINTiMINT-1209490.
STRINGi10090.ENSMUSP00000000939.

Structurei

3D structure databases

ProteinModelPortaliQ9JKY5.
SMRiQ9JKY5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 151ENTHPROSITE-ProRule annotationAdd BLAST129
Domaini771 – 1012I/LWEQPROSITE-ProRule annotationAdd BLAST242

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni867 – 924Important for actin bindingBy similarityAdd BLAST58

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili346 – 644Sequence analysisAdd BLAST299

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi311 – 316Poly-Glu6

Domaini

Binds F-actin via the talin-like I/LWEQ domain.

Sequence similaritiesi

Belongs to the SLA2 family.Curated
Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation
Contains 1 I/LWEQ domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0980. Eukaryota.
ENOG410XRXQ. LUCA.
GeneTreeiENSGT00550000074542.
HOVERGENiHBG005968.
InParanoidiQ9JKY5.
KOiK20040.
OMAiAFQMEQV.
OrthoDBiEOG091G07Y5.
TreeFamiTF316860.

Family and domain databases

Gene3Di1.20.1410.10. 1 hit.
1.25.40.90. 1 hit.
InterProiIPR011417. ANTH_dom.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
IPR032422. HIP1_clath-bd.
IPR030555. HIP1R.
IPR002558. ILWEQ_dom.
IPR030224. Sla2_fam.
[Graphical view]
PANTHERiPTHR10407. PTHR10407. 1 hit.
PTHR10407:SF10. PTHR10407:SF10. 1 hit.
PfamiPF07651. ANTH. 1 hit.
PF16515. HIP1_clath_bdg. 1 hit.
PF01608. I_LWEQ. 1 hit.
[Graphical view]
ProDomiPD011820. ILWEQ. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00273. ENTH. 1 hit.
SM00307. ILWEQ. 1 hit.
[Graphical view]
SUPFAMiSSF109885. SSF109885. 1 hit.
SSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
PS50945. I_LWEQ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JKY5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSIKNVPAR VLSRRPGHSL EAEREQFDKT QAISISKAIN SQEAPVKEKH
60 70 80 90 100
ARRIILGTHH EKGAFTFWSY AIGLPLSSSS ILSWKFCHVL HKVLRDGHPN
110 120 130 140 150
VLHDYQRYRS NIREIGDLWG HLRDQYGHLV NIYTKLLLTK ISFHLKHPQF
160 170 180 190 200
PAGLEVTDEV LEKAAGTDVN NIFQLTVEMF DYMDCELKLS ESVFRQLNTA
210 220 230 240 250
IAVSQMSSGQ CRLAPLIQVI QDCSHLYHYT VKLMFKLHSC LPADTLQGHR
260 270 280 290 300
DRFHEQFHSL KNFFRRASDM LYFKRLIQIP RLPEGPPNFL RASALAEHIK
310 320 330 340 350
PVVVIPEEAP EEEEPENLIE ISSAPPAGEP VVVADLFDQT FGPPNGSMKD
360 370 380 390 400
DRDLQIENLK REVETLRAEL EKIKMEAQRY ISQLKGQVNG LEAELEEQRK
410 420 430 440 450
QKQKALVDNE QLRHELAQLK ALQLEGARNQ GLREEAERKA SATEARYSKL
460 470 480 490 500
KEKHSELINT HAELLRKNAD TAKQLTVTQQ SQEEVARVKE QLAFQMEQAK
510 520 530 540 550
RESEMKMEEQ SDQLEKLKRE LAARAGELAR AQEALSRTEQ SGSELSSRLD
560 570 580 590 600
TLNAEKEALS GVVRQREAEL LAAQSLVREK EEALSQEQQR SSQEKGELRG
610 620 630 640 650
QLAEKESQEQ GLRQKLLDEQ LAVLRSAAAE AEAILQDAVS KLDDPLHLRC
660 670 680 690 700
TSSPDYLVSR AQAALDSVSG LEQGHTQYLA SSEDASALVA ALTRFSHLAA
710 720 730 740 750
DTIVNGAATS HLAPTDPADR LMDTCRECGA RALELVGQLQ DQTVLPRAQP
760 770 780 790 800
SLMRAPLQGI LQLGQDLKPK SLDVRQEELG AMVDKEMAAT SAAIEDAVRR
810 820 830 840 850
IEDMMSQARH ESSGVKLEVN ERILNSCTDL MKAIRLLVMT STSLQKEIVE
860 870 880 890 900
SGRGAATQQE FYAKNSRWTE GLISASKAVG WGATQLVESA DKVVLHMGKY
910 920 930 940 950
EELIVCSHEI AASTAQLVAA SKVKANKNSP HLSRLQECSR TVNERAANVV
960 970 980 990 1000
ASTKSGQEQI EDRDTMDFSG LSLIKLKKQE METQVRVLEL EKTLEAERVR
1010 1020 1030 1040 1050
LGELRKQHYV LAGGMGTPSE EEPSRPSPAP RSGATKKPPL AQKPSIAPRT
1060
DNQLDKKDGV YPAQLVNY
Length:1,068
Mass (Da):119,426
Last modified:July 27, 2011 - v2
Checksum:i992FD4D3FA3F8A6C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti746P → R in AAF34662 (PubMed:10613908).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF221713 mRNA. Translation: AAF34662.1.
AK146663 mRNA. Translation: BAE27341.1.
CCDSiCCDS39276.1.
RefSeqiNP_659507.3. NM_145070.3.
UniGeneiMm.149954.

Genome annotation databases

EnsembliENSMUST00000000939; ENSMUSP00000000939; ENSMUSG00000000915.
GeneIDi29816.
KEGGimmu:29816.
UCSCiuc008zos.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF221713 mRNA. Translation: AAF34662.1.
AK146663 mRNA. Translation: BAE27341.1.
CCDSiCCDS39276.1.
RefSeqiNP_659507.3. NM_145070.3.
UniGeneiMm.149954.

3D structure databases

ProteinModelPortaliQ9JKY5.
SMRiQ9JKY5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205896. 1 interactor.
IntActiQ9JKY5. 8 interactors.
MINTiMINT-1209490.
STRINGi10090.ENSMUSP00000000939.

PTM databases

iPTMnetiQ9JKY5.
PhosphoSitePlusiQ9JKY5.

Proteomic databases

EPDiQ9JKY5.
MaxQBiQ9JKY5.
PaxDbiQ9JKY5.
PRIDEiQ9JKY5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000939; ENSMUSP00000000939; ENSMUSG00000000915.
GeneIDi29816.
KEGGimmu:29816.
UCSCiuc008zos.2. mouse.

Organism-specific databases

CTDi9026.
MGIiMGI:1352504. Hip1r.

Phylogenomic databases

eggNOGiKOG0980. Eukaryota.
ENOG410XRXQ. LUCA.
GeneTreeiENSGT00550000074542.
HOVERGENiHBG005968.
InParanoidiQ9JKY5.
KOiK20040.
OMAiAFQMEQV.
OrthoDBiEOG091G07Y5.
TreeFamiTF316860.

Enzyme and pathway databases

ReactomeiR-MMU-432722. Golgi Associated Vesicle Biogenesis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

ChiTaRSiHip1r. mouse.
PROiQ9JKY5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000000915.
CleanExiMM_HIP1R.
ExpressionAtlasiQ9JKY5. baseline and differential.
GenevisibleiQ9JKY5. MM.

Family and domain databases

Gene3Di1.20.1410.10. 1 hit.
1.25.40.90. 1 hit.
InterProiIPR011417. ANTH_dom.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
IPR032422. HIP1_clath-bd.
IPR030555. HIP1R.
IPR002558. ILWEQ_dom.
IPR030224. Sla2_fam.
[Graphical view]
PANTHERiPTHR10407. PTHR10407. 1 hit.
PTHR10407:SF10. PTHR10407:SF10. 1 hit.
PfamiPF07651. ANTH. 1 hit.
PF16515. HIP1_clath_bdg. 1 hit.
PF01608. I_LWEQ. 1 hit.
[Graphical view]
ProDomiPD011820. ILWEQ. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00273. ENTH. 1 hit.
SM00307. ILWEQ. 1 hit.
[Graphical view]
SUPFAMiSSF109885. SSF109885. 1 hit.
SSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
PS50945. I_LWEQ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHIP1R_MOUSE
AccessioniPrimary (citable) accession number: Q9JKY5
Secondary accession number(s): Q3UJ14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.