Reviewed, UniProtKB/Swiss-Prot Q9JKX6 (NUDT5_MOUSE)

Names and origin

Protein names

Recommended name:
    ADP-sugar pyrophosphatase
    EC=3.6.1.13
    EC=3.6.1.-
Alternative name(s):
    Nucleoside diphosphate-linked moiety X motif 5
      Short name=Nudix motif 5

Gene names

Name:

Nudt5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes with similar activities ADP-ribose and ADP-mannose. Can also hydrolyzes ADP-glucose (40% of ADP-ribose activity) and other nucleotide sugars with low activity (10%).
Catalytic activity	ADP-ribose + H2O = AMP + D-ribose 5-phosphate. ADP-sugar + H2O = AMP + alpha-D-aldose 1-phosphate.
Cofactor	Magnesium.
Tissue specificity	Widely expressed. Most abundant in liver.
Sequence similarities	Belongs to the Nudix hydrolase family.

Ontologies

Keywords
Ligand	Magnesium
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	nucleobase, nucleoside, nucleotide and nucleic acid metabolic process Ref.1 Inferred from direct assay. Source: MGI
Molecular function	ADP-ribose diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-diphosphatase activity Ref.1 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	ADP-sugar pyrophosphatase		PRO_0000057049
Regions
	Motif	96 – 117	22	Nudix box
Amino acid modifications
	Modified residue	9	1	Phosphoserine Ref.5
	Modified residue	10	1	Phosphoserine
Experimental info
	Sequence conflict	103	1	S → N in AAH04571. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9JKX6-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E831852919F85DFE	FASTA	218	23,984
	10 20 30 40 50 60 METRESTESS PGKHLVTSEE LISEGKWVKF EKTTYMDPTG KTRTWETVKL TTRKGKSADA 70 80 90 100 110 120 VSVIPVLQRT LHHECVILVK QFRPPMGSYC LEFPAGFIED GESPEAAALR ELEEETGYKG 130 140 150 160 170 180 EVAECSPAVC MDPGLSNCTT HVVTVTINGD DAGNVRPKPK PGDGEFMEVI SLPKNDLLTR 190 200 210 LDALGAEQHL TVDAKVYAYG LALKHANSKP FEVPFLKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a new member of the Nudix hydrolases from human and mouse." Yang H., Slupska M.M., Wei Y.-F., Tai J.H., Luther W.M., Xia Y.-R., Shih D.M., Chiang J.-H., Baikalov C., Fitz-Gibbon S., Phan I.T., Conrad A., Miller J.H. J. Biol. Chem. 275:8844-8853(2000) [PubMed: 10722730] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: NOD. Tissue: Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 175-180, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, MASS SPECTROMETRY. Tissue: Liver.
[6]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed: 19131326] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF222786 mRNA. Translation: AAF44630.1. AK088222 mRNA. Translation: BAC40220.1. BC004571 mRNA. Translation: AAH04571.1. BC049595 mRNA. Translation: AAH49595.1.
IPI	IPI00123572.
RefSeq	NP_058614.1.
UniGene	Mm.251904
3D structure databases
SMR	Q9JKX6. Positions 13-218.
ModBase	Search...
PTM databases
PhosphoSite	Q9JKX6.
2-D gel databases
REPRODUCTION-2DPAGE	Q9JKX6.
Proteomic databases
PRIDE	Q9JKX6.
Genome annotation databases
Ensembl	ENSMUSG00000025817. Mus musculus. [Contig view]
GeneID	53893.
KEGG	mmu:53893.
Organism-specific databases
MGI	MGI:1858232. Nudt5.
Phylogenomic databases
HOGENOM	Q9JKX6.
HOVERGEN	Q9JKX6.
OMA	Q9JKX6. TRTWETV.
Enzyme and pathway databases
BRENDA	3.6.1.13. 244.
Gene expression databases
ArrayExpress	Q9JKX6.
Bgee	Q9JKX6.
GermOnline	ENSMUSG00000025817. Mus musculus.
Family and domain databases
InterPro	IPR000086. NUDIX_hydrolase_core. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
PROSITE	PS00893. NUDIX. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	310787.
PMAP-CutDB	Q9JKX6.
SOURCE	Search...

Entry information

Entry name

NUDT5_MOUSE

Accession

Primary (citable) accession number: Q9JKX6
Secondary accession number(s): Q99KM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)