Q9JKX4 (AATF_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein AATF

Alternative name(s):
Apoptosis-antagonizing transcription factor
Rb-binding protein Che-1
Traube protein

Gene names

Name:	Aatf
Synonyms:	Che1, Trb

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression By similarity.
Subunit structure	Binds PAWR, POLR2J, RB1/RB, RBL1/P107, RBL2/P130, and SP1. May also bind MAPT By similarity.
Subcellular location	Nucleus › nucleolus Ref.1.
Tissue specificity	Expressed in adrenal gland, brain (Purkinje cells), heart, kidney, liver, lung, muscle, ovary and testis (at the protein level). Ref.1 Ref.5
Developmental stage	Expressed uniformly throughout the embryo until E10.5. From E11.5, the relative expression level increases in the liver, hind brain, spinal cord, dorsal root ganglia, and the posterior commissure. Ref.1
Sequence similarities	Belongs to the AATF family.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from mutant phenotype Ref.1. Source: MGI embryonic cleavage Inferred from mutant phenotype Ref.1. Source: MGI negative regulation of amyloid precursor protein biosynthetic process Inferred from direct assay PubMed 14627703. Source: MGI negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of superoxide anion generation Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara regulation of mitotic cell cycle Inferred from mutant phenotype Ref.1. Source: MGI response to DNA damage stimulus Inferred from electronic annotation. Source: Compara ribosome biogenesis Inferred from mutant phenotype Ref.1. Source: MGI
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: Compara centrosome Inferred from electronic annotation. Source: Compara cytosol Traceable author statement. Source: Reactome focal adhesion Inferred from electronic annotation. Source: Compara nucleolus Inferred from direct assay Ref.1. Source: MGI
Molecular_function	sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9JKX4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9JKX4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     84-84: H → P
     85-199: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9JKX4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     433-458: LLRELIERKTSSLDPNDQVAMGRQWL → VRLFLSFLCYNKPGVCILEPLIVSSG
     459-526: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	Protein AATF		PRO_0000056617
Regions
	Region	240 – 282	43	Binds POLR2J By similarity
	Region	283 – 338	56	Binds RB1 By similarity
	Region	339 – 438	100	Binds RB1 and SP1 By similarity
	Compositional bias	96 – 171	76	Glu-rich
Amino acid modifications
	Modified residue	170	1	Phosphoserine By similarity
	Modified residue	283	1	Phosphoserine By similarity
	Modified residue	287	1	Phosphoserine Ref.6 Ref.7 Ref.8
	Modified residue	288	1	Phosphoserine Ref.6 Ref.7 Ref.8
Natural variations
	Alternative sequence	84	1	H → P in isoform 2.		VSP_014897
	Alternative sequence	85 – 199	115	Missing in isoform 2.		VSP_014898
	Alternative sequence	433 – 458	26	LLREL…GRQWL → VRLFLSFLCYNKPGVCILEP LIVSSG in isoform 3.		VSP_014899
	Alternative sequence	459 – 526	68	Missing in isoform 3.		VSP_014900
Experimental info
	Sequence conflict	2 – 3	2	AA → GR in AAP73747. Ref.5
	Sequence conflict	4 – 5	2	Missing in AAP73747. Ref.5
	Sequence conflict	45	1	E → K in AAP73747. Ref.5
	Sequence conflict	237	1	E → K in AAP73747. Ref.5
	Sequence conflict	247	1	K → Q in AAP73747. Ref.5
	Sequence conflict	258	1	D → G in AAP73747. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8AD2A8396F521DCF	FASTA	526	59,482
	10 20 30 40 50 60 MAAPQPLALQ LEQLLNPRPR EADPEADPEE ATRARVIDRF DEGEEEKDDL AVSSIRKLAP 70 80 90 100 110 120 VSLLDTDKRY SGKTTSRKAW KEDHWEQALP SSSDNEASDE GGSEDGDSEG LGLEEISEDV 130 140 150 160 170 180 DEDLEDNKIS DEGGSEDGDS EGLGLEEFSE DVEEDLEGED EEDREEDRNS EDDGVVAAFS 190 200 210 220 230 240 SVKVSEEVEK GRAVKNQIAL WDQLLEGRIK LQKALLTTNQ LPQPDVFPVF KDKGGPEFAS 250 260 270 280 290 300 ALKNSHKALK ALLRSLVDLQ EELLFQYPDT RHIVNGAKPN TESEEISSED DELVGEKKKQ 310 320 330 340 350 360 RKAPPKRKLE MEDYPSFMAK RFADFTIYRN HTLQKWHDKT KLASGKLGKG FGAFERSILT 370 380 390 400 410 420 QIDHIMMDKE RLLRRTQTKR SAYRVLGKPE PVPEPVAETL PGEPETLPQG PANAHLRDLD 430 440 450 460 470 480 EEIFDDDDFY HQLLRELIER KTSSLDPNDQ VAMGRQWLAI QKLRSKIRKK VDRKASKGRK 490 500 510 520 LRFHVLSKLL SFMAPIDHTA MSDDARTELF RSLFGQLNPP DADRGK
	Isoform 2 [UniParc]. Checksum: 9BFB0EF9774F70ED	FASTA	411	47,085
	10 20 30 40 50 60 MAAPQPLALQ LEQLLNPRPR EADPEADPEE ATRARVIDRF DEGEEEKDDL AVSSIRKLAP 70 80 90 100 110 120 VSLLDTDKRY SGKTTSRKAW KEDPLWDQLL EGRIKLQKAL LTTNQLPQPD VFPVFKDKGG 130 140 150 160 170 180 PEFASALKNS HKALKALLRS LVDLQEELLF QYPDTRHIVN GAKPNTESEE ISSEDDELVG 190 200 210 220 230 240 EKKKQRKAPP KRKLEMEDYP SFMAKRFADF TIYRNHTLQK WHDKTKLASG KLGKGFGAFE 250 260 270 280 290 300 RSILTQIDHI MMDKERLLRR TQTKRSAYRV LGKPEPVPEP VAETLPGEPE TLPQGPANAH 310 320 330 340 350 360 LRDLDEEIFD DDDFYHQLLR ELIERKTSSL DPNDQVAMGR QWLAIQKLRS KIRKKVDRKA 370 380 390 400 410 SKGRKLRFHV LSKLLSFMAP IDHTAMSDDA RTELFRSLFG QLNPPDADRG K
	Isoform 3 [UniParc]. Checksum: D390D787E8B3612A	FASTA	458	51,506
	10 20 30 40 50 60 MAAPQPLALQ LEQLLNPRPR EADPEADPEE ATRARVIDRF DEGEEEKDDL AVSSIRKLAP 70 80 90 100 110 120 VSLLDTDKRY SGKTTSRKAW KEDHWEQALP SSSDNEASDE GGSEDGDSEG LGLEEISEDV 130 140 150 160 170 180 DEDLEDNKIS DEGGSEDGDS EGLGLEEFSE DVEEDLEGED EEDREEDRNS EDDGVVAAFS 190 200 210 220 230 240 SVKVSEEVEK GRAVKNQIAL WDQLLEGRIK LQKALLTTNQ LPQPDVFPVF KDKGGPEFAS 250 260 270 280 290 300 ALKNSHKALK ALLRSLVDLQ EELLFQYPDT RHIVNGAKPN TESEEISSED DELVGEKKKQ 310 320 330 340 350 360 RKAPPKRKLE MEDYPSFMAK RFADFTIYRN HTLQKWHDKT KLASGKLGKG FGAFERSILT 370 380 390 400 410 420 QIDHIMMDKE RLLRRTQTKR SAYRVLGKPE PVPEPVAETL PGEPETLPQG PANAHLRDLD 430 440 450 EEIFDDDDFY HQVRLFLSFL CYNKPGVCIL EPLIVSSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The murine gene, Traube, is essential for the growth of preimplantation embryos." Thomas T., Voss A.K., Petrou P., Gruss P. Dev. Biol. 227:324-342(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: C57BL/6J. Tissue: Thymus.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Mammary tumor.
[5]	"Genomic structure and transcriptional regulation of Che-1, a novel partner of Rb." Monaco L., Passananti C., Fanciulli M. Gene 321:57-63(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24 (ISOFORMS 1/2), NUCLEOTIDE SEQUENCE [MRNA] OF 1-265 (ISOFORM 2), TISSUE SPECIFICITY. Strain: Swiss. Tissue: Testis.
[6]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-287 AND SER-288, MASS SPECTROMETRY. Tissue: Liver.
[7]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-287 AND SER-288, MASS SPECTROMETRY. Tissue: Melanoma.
[8]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-287 AND SER-288, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF222801 mRNA. Translation: AAF26745.1. AK077789 mRNA. Translation: BAC37011.1. AL603708, AL672252 Genomic DNA. Translation: CAI25418.1. AL672252, AL603708 Genomic DNA. Translation: CAI26153.1. BC025080 mRNA. Translation: AAH25080.1. AF322223 Genomic DNA. Translation: AAK07639.1. AY306199 mRNA. Translation: AAP73747.1.
IPI	IPI00123565. IPI00405346. IPI00409289.
RefSeq	NP_062790.1. NM_019816.1.
UniGene	Mm.257482.
3D structure databases
ProteinModelPortal	Q9JKX4.
ModBase	Search...
PTM databases
PhosphoSite	Q9JKX4.
Proteomic databases
PaxDb	Q9JKX4.
PRIDE	Q9JKX4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000018841; ENSMUSP00000018841; ENSMUSG00000018697.
GeneID	56321.
KEGG	mmu:56321.
UCSC	uc007kqm.1. mouse. uc007kqn.1. mouse.
Organism-specific databases
CTD	26574.
MGI	MGI:1929608. Aatf.
Phylogenomic databases
eggNOG	NOG270454.
GeneTree	ENSGT00390000000288.
HOGENOM	HOG000007555.
HOVERGEN	HBG080805.
InParanoid	Q9JKX4.
KO	K14782.
OMA	DKGGPEF.
OrthoDB	EOG41NTM1.
Enzyme and pathway databases
Reactome	REACT_115202. Signal Transduction.
Gene expression databases
Bgee	Q9JKX4.
CleanEx	MM_AATF.
Genevestigator	Q9JKX4.
GermOnline	ENSMUSG00000018697. Mus musculus.
Family and domain databases
InterPro	IPR025160. AATF. IPR012617. AATF_C. [Graphical view]
Pfam	PF13339. AATF-Che1. 1 hit. PF08164. TRAUB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	312286.
SOURCE	Search...

Entry information

Entry name

AATF_MOUSE

Accession

Primary (citable) accession number: Q9JKX4
Secondary accession number(s): Q7TQN1, Q8C5Q2, Q99P89

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program