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Protein

Protein AATF

Gene

Aatf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cell adhesion Source: MGI
  • cellular response to DNA damage stimulus Source: Ensembl
  • embryonic cleavage Source: MGI
  • negative regulation of amyloid precursor protein biosynthetic process Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of reactive oxygen species metabolic process Source: MGI
  • negative regulation of superoxide anion generation Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • regulation of mitotic cell cycle Source: MGI
  • regulation of transcription by RNA polymerase II Source: GO_Central
  • ribosome biogenesis Source: MGI

Names & Taxonomyi

Protein namesi
Recommended name:
Protein AATF
Alternative name(s):
Apoptosis-antagonizing transcription factor
Rb-binding protein Che-1
Traube protein
Gene namesi
Name:Aatf
Synonyms:Che1, Trb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1929608 Aatf

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000566172 – 526Protein AATFAdd BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei62PhosphoserineBy similarity1
Modified residuei170PhosphoserineBy similarity1
Modified residuei240PhosphoserineBy similarity1
Modified residuei283PhosphoserineCombined sources1
Modified residuei287PhosphoserineCombined sources1
Modified residuei288PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9JKX4
PaxDbiQ9JKX4
PeptideAtlasiQ9JKX4
PRIDEiQ9JKX4

PTM databases

iPTMnetiQ9JKX4
PhosphoSitePlusiQ9JKX4

Expressioni

Tissue specificityi

Expressed in adrenal gland, brain (Purkinje cells), heart, kidney, liver, lung, muscle, ovary and testis (at the protein level).2 Publications

Developmental stagei

Expressed uniformly throughout the embryo until E10.5. From E11.5, the relative expression level increases in the liver, hind brain, spinal cord, dorsal root ganglia, and the posterior commissure.1 Publication

Gene expression databases

BgeeiENSMUSG00000018697
CleanExiMM_AATF
GenevisibleiQ9JKX4 MM

Interactioni

Subunit structurei

Binds PAWR, POLR2J, RB1/RB, RBL1/P107, RBL2/P130, and SP1. May also bind MAPT (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207901, 3 interactors
IntActiQ9JKX4, 1 interactor
MINTiQ9JKX4
STRINGi10090.ENSMUSP00000018841

Structurei

3D structure databases

ProteinModelPortaliQ9JKX4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni240 – 282Binds POLR2JBy similarityAdd BLAST43
Regioni283 – 338Binds RB1By similarityAdd BLAST56
Regioni339 – 438Binds RB1 and SP1By similarityAdd BLAST100

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi96 – 171Glu-richAdd BLAST76

Sequence similaritiesi

Belongs to the AATF family.Curated

Phylogenomic databases

eggNOGiKOG2773 Eukaryota
ENOG410XWGK LUCA
GeneTreeiENSGT00390000000288
HOGENOMiHOG000007555
HOVERGENiHBG080805
InParanoidiQ9JKX4
KOiK14782
OMAiEVLLKWD
OrthoDBiEOG091G0F1H
PhylomeDBiQ9JKX4
TreeFamiTF324341

Family and domain databases

InterProiView protein in InterPro
IPR025160 AATF
IPR012617 AATF_C
PfamiView protein in Pfam
PF13339 AATF-Che1, 1 hit
PF08164 TRAUB, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JKX4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPQPLALQ LEQLLNPRPR EADPEADPEE ATRARVIDRF DEGEEEKDDL
60 70 80 90 100
AVSSIRKLAP VSLLDTDKRY SGKTTSRKAW KEDHWEQALP SSSDNEASDE
110 120 130 140 150
GGSEDGDSEG LGLEEISEDV DEDLEDNKIS DEGGSEDGDS EGLGLEEFSE
160 170 180 190 200
DVEEDLEGED EEDREEDRNS EDDGVVAAFS SVKVSEEVEK GRAVKNQIAL
210 220 230 240 250
WDQLLEGRIK LQKALLTTNQ LPQPDVFPVF KDKGGPEFAS ALKNSHKALK
260 270 280 290 300
ALLRSLVDLQ EELLFQYPDT RHIVNGAKPN TESEEISSED DELVGEKKKQ
310 320 330 340 350
RKAPPKRKLE MEDYPSFMAK RFADFTIYRN HTLQKWHDKT KLASGKLGKG
360 370 380 390 400
FGAFERSILT QIDHIMMDKE RLLRRTQTKR SAYRVLGKPE PVPEPVAETL
410 420 430 440 450
PGEPETLPQG PANAHLRDLD EEIFDDDDFY HQLLRELIER KTSSLDPNDQ
460 470 480 490 500
VAMGRQWLAI QKLRSKIRKK VDRKASKGRK LRFHVLSKLL SFMAPIDHTA
510 520
MSDDARTELF RSLFGQLNPP DADRGK
Length:526
Mass (Da):59,482
Last modified:October 1, 2000 - v1
Checksum:i8AD2A8396F521DCF
GO
Isoform 2 (identifier: Q9JKX4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     84-84: H → P
     85-199: Missing.

Note: No experimental confirmation available.
Show »
Length:411
Mass (Da):47,085
Checksum:i9BFB0EF9774F70ED
GO
Isoform 3 (identifier: Q9JKX4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     433-458: LLRELIERKTSSLDPNDQVAMGRQWL → VRLFLSFLCYNKPGVCILEPLIVSSG
     459-526: Missing.

Show »
Length:458
Mass (Da):51,506
Checksum:iD390D787E8B3612A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2 – 3AA → GR in AAP73747 (PubMed:14636992).Curated2
Sequence conflicti4 – 5Missing in AAP73747 (PubMed:14636992).Curated2
Sequence conflicti45E → K in AAP73747 (PubMed:14636992).Curated1
Sequence conflicti237E → K in AAP73747 (PubMed:14636992).Curated1
Sequence conflicti247K → Q in AAP73747 (PubMed:14636992).Curated1
Sequence conflicti258D → G in AAP73747 (PubMed:14636992).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01489784H → P in isoform 2. 1 Publication1
Alternative sequenceiVSP_01489885 – 199Missing in isoform 2. 1 PublicationAdd BLAST115
Alternative sequenceiVSP_014899433 – 458LLREL…GRQWL → VRLFLSFLCYNKPGVCILEP LIVSSG in isoform 3. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_014900459 – 526Missing in isoform 3. 1 PublicationAdd BLAST68

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF222801 mRNA Translation: AAF26745.1
AK077789 mRNA Translation: BAC37011.1
AL603708, AL672252 Genomic DNA Translation: CAI25418.1
AL672252, AL603708 Genomic DNA Translation: CAI26153.1
BC025080 mRNA Translation: AAH25080.1
AF322223 Genomic DNA Translation: AAK07639.1
AY306199 mRNA Translation: AAP73747.1
CCDSiCCDS25186.1 [Q9JKX4-1]
RefSeqiNP_062790.1, NM_019816.1 [Q9JKX4-1]
UniGeneiMm.257482

Genome annotation databases

EnsembliENSMUST00000018841; ENSMUSP00000018841; ENSMUSG00000018697 [Q9JKX4-1]
GeneIDi56321
KEGGimmu:56321
UCSCiuc007kqm.1 mouse [Q9JKX4-1]
uc007kqn.1 mouse [Q9JKX4-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAATF_MOUSE
AccessioniPrimary (citable) accession number: Q9JKX4
Secondary accession number(s): Q7TQN1, Q8C5Q2, Q99P89
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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