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Q9JKX4

- AATF_MOUSE

UniProt

Q9JKX4 - AATF_MOUSE

Protein

Protein AATF

Gene

Aatf

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. sequence-specific DNA binding transcription factor activity Source: Ensembl

    GO - Biological processi

    1. cell adhesion Source: MGI
    2. cellular response to DNA damage stimulus Source: Ensembl
    3. embryonic cleavage Source: MGI
    4. negative regulation of amyloid precursor protein biosynthetic process Source: MGI
    5. negative regulation of apoptotic process Source: Ensembl
    6. negative regulation of superoxide anion generation Source: Ensembl
    7. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    8. regulation of mitotic cell cycle Source: MGI
    9. ribosome biogenesis Source: MGI

    Enzyme and pathway databases

    ReactomeiREACT_209641. NRAGE signals death through JNK.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein AATF
    Alternative name(s):
    Apoptosis-antagonizing transcription factor
    Rb-binding protein Che-1
    Traube protein
    Gene namesi
    Name:Aatf
    Synonyms:Che1, Trb
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1929608. Aatf.

    Subcellular locationi

    Nucleusnucleolus 1 Publication

    GO - Cellular componenti

    1. centrosome Source: Ensembl
    2. cytoplasm Source: MGI
    3. cytosol Source: Reactome
    4. focal adhesion Source: Ensembl
    5. Golgi apparatus Source: Ensembl
    6. nucleolus Source: MGI
    7. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 526525Protein AATFPRO_0000056617Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei170 – 1701PhosphoserineBy similarity
    Modified residuei283 – 2831PhosphoserineBy similarity
    Modified residuei287 – 2871Phosphoserine1 Publication
    Modified residuei288 – 2881Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9JKX4.
    PaxDbiQ9JKX4.
    PRIDEiQ9JKX4.

    PTM databases

    PhosphoSiteiQ9JKX4.

    Expressioni

    Tissue specificityi

    Expressed in adrenal gland, brain (Purkinje cells), heart, kidney, liver, lung, muscle, ovary and testis (at the protein level).2 Publications

    Developmental stagei

    Expressed uniformly throughout the embryo until E10.5. From E11.5, the relative expression level increases in the liver, hind brain, spinal cord, dorsal root ganglia, and the posterior commissure.1 Publication

    Gene expression databases

    BgeeiQ9JKX4.
    CleanExiMM_AATF.
    GenevestigatoriQ9JKX4.

    Interactioni

    Subunit structurei

    Binds PAWR, POLR2J, RB1/RB, RBL1/P107, RBL2/P130, and SP1. May also bind MAPT By similarity.By similarity

    Protein-protein interaction databases

    BioGridi207901. 2 interactions.
    IntActiQ9JKX4. 1 interaction.
    MINTiMINT-4130159.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JKX4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni240 – 28243Binds POLR2JBy similarityAdd
    BLAST
    Regioni283 – 33856Binds RB1By similarityAdd
    BLAST
    Regioni339 – 438100Binds RB1 and SP1By similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi96 – 17176Glu-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the AATF family.Curated

    Phylogenomic databases

    eggNOGiNOG270454.
    GeneTreeiENSGT00390000000288.
    HOGENOMiHOG000007555.
    HOVERGENiHBG080805.
    InParanoidiQ9JKX4.
    KOiK14782.
    OMAiQLHPPDE.
    OrthoDBiEOG7VDXQ2.
    PhylomeDBiQ9JKX4.
    TreeFamiTF324341.

    Family and domain databases

    InterProiIPR025160. AATF.
    IPR012617. AATF_C.
    [Graphical view]
    PfamiPF13339. AATF-Che1. 1 hit.
    PF08164. TRAUB. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9JKX4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAPQPLALQ LEQLLNPRPR EADPEADPEE ATRARVIDRF DEGEEEKDDL    50
    AVSSIRKLAP VSLLDTDKRY SGKTTSRKAW KEDHWEQALP SSSDNEASDE 100
    GGSEDGDSEG LGLEEISEDV DEDLEDNKIS DEGGSEDGDS EGLGLEEFSE 150
    DVEEDLEGED EEDREEDRNS EDDGVVAAFS SVKVSEEVEK GRAVKNQIAL 200
    WDQLLEGRIK LQKALLTTNQ LPQPDVFPVF KDKGGPEFAS ALKNSHKALK 250
    ALLRSLVDLQ EELLFQYPDT RHIVNGAKPN TESEEISSED DELVGEKKKQ 300
    RKAPPKRKLE MEDYPSFMAK RFADFTIYRN HTLQKWHDKT KLASGKLGKG 350
    FGAFERSILT QIDHIMMDKE RLLRRTQTKR SAYRVLGKPE PVPEPVAETL 400
    PGEPETLPQG PANAHLRDLD EEIFDDDDFY HQLLRELIER KTSSLDPNDQ 450
    VAMGRQWLAI QKLRSKIRKK VDRKASKGRK LRFHVLSKLL SFMAPIDHTA 500
    MSDDARTELF RSLFGQLNPP DADRGK 526
    Length:526
    Mass (Da):59,482
    Last modified:October 1, 2000 - v1
    Checksum:i8AD2A8396F521DCF
    GO
    Isoform 2 (identifier: Q9JKX4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         84-84: H → P
         85-199: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:411
    Mass (Da):47,085
    Checksum:i9BFB0EF9774F70ED
    GO
    Isoform 3 (identifier: Q9JKX4-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         433-458: LLRELIERKTSSLDPNDQVAMGRQWL → VRLFLSFLCYNKPGVCILEPLIVSSG
         459-526: Missing.

    Show »
    Length:458
    Mass (Da):51,506
    Checksum:iD390D787E8B3612A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 32AA → GR in AAP73747. (PubMed:14636992)Curated
    Sequence conflicti4 – 52Missing in AAP73747. (PubMed:14636992)Curated
    Sequence conflicti45 – 451E → K in AAP73747. (PubMed:14636992)Curated
    Sequence conflicti237 – 2371E → K in AAP73747. (PubMed:14636992)Curated
    Sequence conflicti247 – 2471K → Q in AAP73747. (PubMed:14636992)Curated
    Sequence conflicti258 – 2581D → G in AAP73747. (PubMed:14636992)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei84 – 841H → P in isoform 2. 1 PublicationVSP_014897
    Alternative sequencei85 – 199115Missing in isoform 2. 1 PublicationVSP_014898Add
    BLAST
    Alternative sequencei433 – 45826LLREL…GRQWL → VRLFLSFLCYNKPGVCILEP LIVSSG in isoform 3. 1 PublicationVSP_014899Add
    BLAST
    Alternative sequencei459 – 52668Missing in isoform 3. 1 PublicationVSP_014900Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF222801 mRNA. Translation: AAF26745.1.
    AK077789 mRNA. Translation: BAC37011.1.
    AL603708, AL672252 Genomic DNA. Translation: CAI25418.1.
    AL672252, AL603708 Genomic DNA. Translation: CAI26153.1.
    BC025080 mRNA. Translation: AAH25080.1.
    AF322223 Genomic DNA. Translation: AAK07639.1.
    AY306199 mRNA. Translation: AAP73747.1.
    CCDSiCCDS25186.1. [Q9JKX4-1]
    RefSeqiNP_062790.1. NM_019816.1. [Q9JKX4-1]
    UniGeneiMm.257482.

    Genome annotation databases

    EnsembliENSMUST00000018841; ENSMUSP00000018841; ENSMUSG00000018697. [Q9JKX4-1]
    GeneIDi56321.
    KEGGimmu:56321.
    UCSCiuc007kqm.1. mouse. [Q9JKX4-1]
    uc007kqn.1. mouse. [Q9JKX4-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF222801 mRNA. Translation: AAF26745.1 .
    AK077789 mRNA. Translation: BAC37011.1 .
    AL603708 , AL672252 Genomic DNA. Translation: CAI25418.1 .
    AL672252 , AL603708 Genomic DNA. Translation: CAI26153.1 .
    BC025080 mRNA. Translation: AAH25080.1 .
    AF322223 Genomic DNA. Translation: AAK07639.1 .
    AY306199 mRNA. Translation: AAP73747.1 .
    CCDSi CCDS25186.1. [Q9JKX4-1 ]
    RefSeqi NP_062790.1. NM_019816.1. [Q9JKX4-1 ]
    UniGenei Mm.257482.

    3D structure databases

    ProteinModelPortali Q9JKX4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 207901. 2 interactions.
    IntActi Q9JKX4. 1 interaction.
    MINTi MINT-4130159.

    PTM databases

    PhosphoSitei Q9JKX4.

    Proteomic databases

    MaxQBi Q9JKX4.
    PaxDbi Q9JKX4.
    PRIDEi Q9JKX4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000018841 ; ENSMUSP00000018841 ; ENSMUSG00000018697 . [Q9JKX4-1 ]
    GeneIDi 56321.
    KEGGi mmu:56321.
    UCSCi uc007kqm.1. mouse. [Q9JKX4-1 ]
    uc007kqn.1. mouse. [Q9JKX4-3 ]

    Organism-specific databases

    CTDi 26574.
    MGIi MGI:1929608. Aatf.

    Phylogenomic databases

    eggNOGi NOG270454.
    GeneTreei ENSGT00390000000288.
    HOGENOMi HOG000007555.
    HOVERGENi HBG080805.
    InParanoidi Q9JKX4.
    KOi K14782.
    OMAi QLHPPDE.
    OrthoDBi EOG7VDXQ2.
    PhylomeDBi Q9JKX4.
    TreeFami TF324341.

    Enzyme and pathway databases

    Reactomei REACT_209641. NRAGE signals death through JNK.

    Miscellaneous databases

    NextBioi 312286.
    PROi Q9JKX4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9JKX4.
    CleanExi MM_AATF.
    Genevestigatori Q9JKX4.

    Family and domain databases

    InterProi IPR025160. AATF.
    IPR012617. AATF_C.
    [Graphical view ]
    Pfami PF13339. AATF-Che1. 1 hit.
    PF08164. TRAUB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The murine gene, Traube, is essential for the growth of preimplantation embryos."
      Thomas T., Voss A.K., Petrou P., Gruss P.
      Dev. Biol. 227:324-342(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Strain: C57BL/6J.
      Tissue: Thymus.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Mammary tumor.
    5. "Genomic structure and transcriptional regulation of Che-1, a novel partner of Rb."
      Monaco L., Passananti C., Fanciulli M.
      Gene 321:57-63(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24 (ISOFORMS 1/2), NUCLEOTIDE SEQUENCE [MRNA] OF 1-265 (ISOFORM 2), TISSUE SPECIFICITY.
      Strain: Swiss.
      Tissue: Testis.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-287 AND SER-288, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiAATF_MOUSE
    AccessioniPrimary (citable) accession number: Q9JKX4
    Secondary accession number(s): Q7TQN1, Q8C5Q2, Q99P89
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2005
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3