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Q9JKX4

- AATF_MOUSE

UniProt

Q9JKX4 - AATF_MOUSE

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Protein

Protein AATF

Gene

Aatf

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression (By similarity).By similarity

GO - Molecular functioni

  1. poly(A) RNA binding Source: Ensembl
  2. sequence-specific DNA binding transcription factor activity Source: Ensembl

GO - Biological processi

  1. cell adhesion Source: MGI
  2. cellular response to DNA damage stimulus Source: Ensembl
  3. embryonic cleavage Source: MGI
  4. negative regulation of amyloid precursor protein biosynthetic process Source: MGI
  5. negative regulation of apoptotic process Source: Ensembl
  6. negative regulation of superoxide anion generation Source: Ensembl
  7. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  8. regulation of mitotic cell cycle Source: MGI
  9. ribosome biogenesis Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_209641. NRAGE signals death through JNK.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein AATF
Alternative name(s):
Apoptosis-antagonizing transcription factor
Rb-binding protein Che-1
Traube protein
Gene namesi
Name:Aatf
Synonyms:Che1, Trb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1929608. Aatf.

Subcellular locationi

Nucleusnucleolus 1 Publication

GO - Cellular componenti

  1. centrosome Source: Ensembl
  2. cytoplasm Source: MGI
  3. cytosol Source: Reactome
  4. focal adhesion Source: Ensembl
  5. Golgi apparatus Source: Ensembl
  6. nucleolus Source: MGI
  7. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 526525Protein AATFPRO_0000056617Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei170 – 1701PhosphoserineBy similarity
Modified residuei283 – 2831PhosphoserineBy similarity
Modified residuei287 – 2871Phosphoserine1 Publication
Modified residuei288 – 2881Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9JKX4.
PaxDbiQ9JKX4.
PRIDEiQ9JKX4.

PTM databases

PhosphoSiteiQ9JKX4.

Expressioni

Tissue specificityi

Expressed in adrenal gland, brain (Purkinje cells), heart, kidney, liver, lung, muscle, ovary and testis (at the protein level).2 Publications

Developmental stagei

Expressed uniformly throughout the embryo until E10.5. From E11.5, the relative expression level increases in the liver, hind brain, spinal cord, dorsal root ganglia, and the posterior commissure.1 Publication

Gene expression databases

BgeeiQ9JKX4.
CleanExiMM_AATF.
GenevestigatoriQ9JKX4.

Interactioni

Subunit structurei

Binds PAWR, POLR2J, RB1/RB, RBL1/P107, RBL2/P130, and SP1. May also bind MAPT (By similarity).By similarity

Protein-protein interaction databases

BioGridi207901. 2 interactions.
IntActiQ9JKX4. 1 interaction.
MINTiMINT-4130159.

Structurei

3D structure databases

ProteinModelPortaliQ9JKX4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni240 – 28243Binds POLR2JBy similarityAdd
BLAST
Regioni283 – 33856Binds RB1By similarityAdd
BLAST
Regioni339 – 438100Binds RB1 and SP1By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi96 – 17176Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the AATF family.Curated

Phylogenomic databases

eggNOGiNOG270454.
GeneTreeiENSGT00390000000288.
HOGENOMiHOG000007555.
HOVERGENiHBG080805.
InParanoidiQ9JKX4.
KOiK14782.
OMAiQLHPPDE.
OrthoDBiEOG7VDXQ2.
PhylomeDBiQ9JKX4.
TreeFamiTF324341.

Family and domain databases

InterProiIPR025160. AATF.
IPR012617. AATF_C.
[Graphical view]
PfamiPF13339. AATF-Che1. 1 hit.
PF08164. TRAUB. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9JKX4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPQPLALQ LEQLLNPRPR EADPEADPEE ATRARVIDRF DEGEEEKDDL
60 70 80 90 100
AVSSIRKLAP VSLLDTDKRY SGKTTSRKAW KEDHWEQALP SSSDNEASDE
110 120 130 140 150
GGSEDGDSEG LGLEEISEDV DEDLEDNKIS DEGGSEDGDS EGLGLEEFSE
160 170 180 190 200
DVEEDLEGED EEDREEDRNS EDDGVVAAFS SVKVSEEVEK GRAVKNQIAL
210 220 230 240 250
WDQLLEGRIK LQKALLTTNQ LPQPDVFPVF KDKGGPEFAS ALKNSHKALK
260 270 280 290 300
ALLRSLVDLQ EELLFQYPDT RHIVNGAKPN TESEEISSED DELVGEKKKQ
310 320 330 340 350
RKAPPKRKLE MEDYPSFMAK RFADFTIYRN HTLQKWHDKT KLASGKLGKG
360 370 380 390 400
FGAFERSILT QIDHIMMDKE RLLRRTQTKR SAYRVLGKPE PVPEPVAETL
410 420 430 440 450
PGEPETLPQG PANAHLRDLD EEIFDDDDFY HQLLRELIER KTSSLDPNDQ
460 470 480 490 500
VAMGRQWLAI QKLRSKIRKK VDRKASKGRK LRFHVLSKLL SFMAPIDHTA
510 520
MSDDARTELF RSLFGQLNPP DADRGK
Length:526
Mass (Da):59,482
Last modified:October 1, 2000 - v1
Checksum:i8AD2A8396F521DCF
GO
Isoform 2 (identifier: Q9JKX4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     84-84: H → P
     85-199: Missing.

Note: No experimental confirmation available.

Show »
Length:411
Mass (Da):47,085
Checksum:i9BFB0EF9774F70ED
GO
Isoform 3 (identifier: Q9JKX4-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     433-458: LLRELIERKTSSLDPNDQVAMGRQWL → VRLFLSFLCYNKPGVCILEPLIVSSG
     459-526: Missing.

Show »
Length:458
Mass (Da):51,506
Checksum:iD390D787E8B3612A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 32AA → GR in AAP73747. (PubMed:14636992)Curated
Sequence conflicti4 – 52Missing in AAP73747. (PubMed:14636992)Curated
Sequence conflicti45 – 451E → K in AAP73747. (PubMed:14636992)Curated
Sequence conflicti237 – 2371E → K in AAP73747. (PubMed:14636992)Curated
Sequence conflicti247 – 2471K → Q in AAP73747. (PubMed:14636992)Curated
Sequence conflicti258 – 2581D → G in AAP73747. (PubMed:14636992)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei84 – 841H → P in isoform 2. 1 PublicationVSP_014897
Alternative sequencei85 – 199115Missing in isoform 2. 1 PublicationVSP_014898Add
BLAST
Alternative sequencei433 – 45826LLREL…GRQWL → VRLFLSFLCYNKPGVCILEP LIVSSG in isoform 3. 1 PublicationVSP_014899Add
BLAST
Alternative sequencei459 – 52668Missing in isoform 3. 1 PublicationVSP_014900Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF222801 mRNA. Translation: AAF26745.1.
AK077789 mRNA. Translation: BAC37011.1.
AL603708, AL672252 Genomic DNA. Translation: CAI25418.1.
AL672252, AL603708 Genomic DNA. Translation: CAI26153.1.
BC025080 mRNA. Translation: AAH25080.1.
AF322223 Genomic DNA. Translation: AAK07639.1.
AY306199 mRNA. Translation: AAP73747.1.
CCDSiCCDS25186.1. [Q9JKX4-1]
RefSeqiNP_062790.1. NM_019816.1. [Q9JKX4-1]
UniGeneiMm.257482.

Genome annotation databases

EnsembliENSMUST00000018841; ENSMUSP00000018841; ENSMUSG00000018697. [Q9JKX4-1]
GeneIDi56321.
KEGGimmu:56321.
UCSCiuc007kqm.1. mouse. [Q9JKX4-1]
uc007kqn.1. mouse. [Q9JKX4-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF222801 mRNA. Translation: AAF26745.1 .
AK077789 mRNA. Translation: BAC37011.1 .
AL603708 , AL672252 Genomic DNA. Translation: CAI25418.1 .
AL672252 , AL603708 Genomic DNA. Translation: CAI26153.1 .
BC025080 mRNA. Translation: AAH25080.1 .
AF322223 Genomic DNA. Translation: AAK07639.1 .
AY306199 mRNA. Translation: AAP73747.1 .
CCDSi CCDS25186.1. [Q9JKX4-1 ]
RefSeqi NP_062790.1. NM_019816.1. [Q9JKX4-1 ]
UniGenei Mm.257482.

3D structure databases

ProteinModelPortali Q9JKX4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 207901. 2 interactions.
IntActi Q9JKX4. 1 interaction.
MINTi MINT-4130159.

PTM databases

PhosphoSitei Q9JKX4.

Proteomic databases

MaxQBi Q9JKX4.
PaxDbi Q9JKX4.
PRIDEi Q9JKX4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000018841 ; ENSMUSP00000018841 ; ENSMUSG00000018697 . [Q9JKX4-1 ]
GeneIDi 56321.
KEGGi mmu:56321.
UCSCi uc007kqm.1. mouse. [Q9JKX4-1 ]
uc007kqn.1. mouse. [Q9JKX4-3 ]

Organism-specific databases

CTDi 26574.
MGIi MGI:1929608. Aatf.

Phylogenomic databases

eggNOGi NOG270454.
GeneTreei ENSGT00390000000288.
HOGENOMi HOG000007555.
HOVERGENi HBG080805.
InParanoidi Q9JKX4.
KOi K14782.
OMAi QLHPPDE.
OrthoDBi EOG7VDXQ2.
PhylomeDBi Q9JKX4.
TreeFami TF324341.

Enzyme and pathway databases

Reactomei REACT_209641. NRAGE signals death through JNK.

Miscellaneous databases

NextBioi 312286.
PROi Q9JKX4.
SOURCEi Search...

Gene expression databases

Bgeei Q9JKX4.
CleanExi MM_AATF.
Genevestigatori Q9JKX4.

Family and domain databases

InterProi IPR025160. AATF.
IPR012617. AATF_C.
[Graphical view ]
Pfami PF13339. AATF-Che1. 1 hit.
PF08164. TRAUB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The murine gene, Traube, is essential for the growth of preimplantation embryos."
    Thomas T., Voss A.K., Petrou P., Gruss P.
    Dev. Biol. 227:324-342(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Thymus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  5. "Genomic structure and transcriptional regulation of Che-1, a novel partner of Rb."
    Monaco L., Passananti C., Fanciulli M.
    Gene 321:57-63(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24 (ISOFORMS 1/2), NUCLEOTIDE SEQUENCE [MRNA] OF 1-265 (ISOFORM 2), TISSUE SPECIFICITY.
    Strain: Swiss.
    Tissue: Testis.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-287 AND SER-288, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiAATF_MOUSE
AccessioniPrimary (citable) accession number: Q9JKX4
Secondary accession number(s): Q7TQN1, Q8C5Q2, Q99P89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3