Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9JKX3 (TFR2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transferrin receptor protein 2

Short name=TfR2
Gene names
Name:Tfr2
Synonyms:Trfr2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length798 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Mediates cellular uptake of transferrin-bound iron in a non-iron dependent manner. May be involved in iron metabolism, hepatocyte function and erythrocyte differentiation.

Subcellular location

Cell membrane; Single-pass type II membrane protein.

Isoform 3: Cytoplasm.

Tissue specificity

Predominantly expressed in liver. Also expressed in kidney, spleen, brain, lung, heart and muscle with very low expression in kidney, muscle and heart.

Developmental stage

First expressed between embryo days 8 and 11. In the liver, expression increases during development from embryo day 13 to adulthood while, in the spleen, levels remain constant throughout development.

Induction

Down-regulated during erythrocyte differentiation. Expression unchanged by cellular iron status.

Sequence similarities

Belongs to the peptidase M28 family. M28B subfamily.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9JKX3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9JKX3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     237-237: T → TVRFPGWGAHHVLIG
     238-798: Missing.
Note: Lacks most of the extracellular domain. No experimental confirmation available.
Isoform 3 (identifier: Q9JKX3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     12-93: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 798798Transferrin receptor protein 2
PRO_0000174137

Regions

Topological domain1 – 8181Cytoplasmic Potential
Transmembrane82 – 10221Helical; Signal-anchor for type II membrane protein; Potential
Topological domain103 – 798696Extracellular Potential
Motif23 – 264Endocytosis signal Potential

Amino acid modifications

Glycosylation2351N-linked (GlcNAc...) Potential
Glycosylation3341N-linked (GlcNAc...) Potential
Glycosylation5351N-linked (GlcNAc...) Potential
Disulfide bond106Interchain Potential
Disulfide bond109Interchain Potential

Natural variations

Alternative sequence12 – 9382Missing in isoform 3.
VSP_005356
Alternative sequence2371T → TVRFPGWGAHHVLIG in isoform 2.
VSP_005357
Alternative sequence238 – 798561Missing in isoform 2.
VSP_005358

Experimental info

Sequence conflict251R → P in AAL05977. Ref.2
Sequence conflict421G → V in AAL05977. Ref.2
Sequence conflict421G → V in AAK28830. Ref.5
Sequence conflict1031R → P in AAL05977. Ref.2
Sequence conflict1511T → N in AAH13654. Ref.4
Sequence conflict2481S → L in AAL05976. Ref.2
Sequence conflict2871A → V in AAL05976. Ref.2
Sequence conflict5951K → E in AAF37272. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 11, 2002. Version 2.
Checksum: FA6161FE3FFF2AA4

FASTA79888,402
        10         20         30         40         50         60 
MEQRWGLLRR VQQWSPRPSQ TIYRRVEGPQ LEHLEEEDRE EGAELPAQFC PMELKGPEHL 

        70         80         90        100        110        120 
GSCPGRSIPI PWAAAGRKAA PYLVLITLLI FTGAFLLGYV AFRGSCQACG DSVLVVDEDV 

       130        140        150        160        170        180 
NPEDSGRTTL YWSDLQAMFL RFLGEGRMED TIRLTSLRER VAGSARMATL VQDILDKLSR 

       190        200        210        220        230        240 
QKLDHVWTDT HYVGLQFPDP AHANTLHWVD ADGSVQEQLP LEDPEVYCPY SATGNATGKL 

       250        260        270        280        290        300 
VYAHYGRSED LQDLKAKGVE LAGSLLLVRV GITSFAQKVA VAQDFGAQGV LIYPDPSDFS 

       310        320        330        340        350        360 
QDPHKPGLSS HQAVYGHVHL GTGDPYTPGF PSFNQTQFPP VESSGLPSIP AQPISADIAD 

       370        380        390        400        410        420 
QLLRKLTGPV APQEWKGHLS GSPYRLGPGP DLRLVVNNHR VSTPISNIFA CIEGFAEPDH 

       430        440        450        460        470        480 
YVVIGAQRDA WGPGAAKSAV GTAILLELVR TFSSMVSNGF RPRRSLLFIS WDGGDFGSVG 

       490        500        510        520        530        540 
ATEWLEGYLS VLHLKAVVYV SLDNSVLGDG KFHAKTSPLL VSLIENILKQ VDSPNHSGQT 

       550        560        570        580        590        600 
LYEQVALTHP SWDAEVIQPL PMDSSAYSFT AFAGVPAVEF SFMEDDRVYP FLHTKEDTYE 

       610        620        630        640        650        660 
NLHKMLRGRL PAVVQAVAQL AGQLLIRLSH DHLLPLDFGR YGDVVLRHIG NLNEFSGDLK 

       670        680        690        700        710        720 
ERGLTLQWVY SARGDYIRAA EKLRKEIYSS ERNDERLMRM YNVRIMRVEF YFLSQYVSPA 

       730        740        750        760        770        780 
DSPFRHIFLG QGDHTLGALV DHLRMLRADG SGAASSRLTA GLGFQESRFR RQLALLTWTL 

       790 
QGAANALSGD VWNIDNNF 

« Hide

Isoform 2 [UniParc].

Checksum: 2A991D52876D5C18
Show »

FASTA25128,167
Isoform 3 [UniParc].

Checksum: CFDA8CA8F8723197
Show »

FASTA71679,255

References

« Hide 'large scale' references
[1]"Transferrin receptor 2: continued expression in mouse liver in the face of iron overload and in hereditary hemochromatosis."
Fleming R.E., Migas M.C., Holden C.C., Waheed A., Britton R.S., Tomatsu S., Bacon B.R., Sly W.S.
Proc. Natl. Acad. Sci. U.S.A. 97:2214-2219(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Embryo.
[2]"Regulation of expression of murine transferrin receptor 2."
Kawabata H., Germain R.S., Ikezoe T., Tong X., Green E.M., Gombart A.F., Koeffler H.P.
Blood 98:1949-1954(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
Tissue: Erythroleukemia.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Liver.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[5]"Comparative analysis of the gene-dense ACHE/TFR2 region on human chromosome 7q22 with the orthologous region on mouse chromosome 5."
Wilson M.D., Riemer C., Martindale D.W., Schnupf P., Boright A.P., Cheung T.L., Hardy D.M., Schwartz S., Scherer S.W., Tsui L.-C., Miller W., Koop B.F.
Nucleic Acids Res. 29:1352-1365(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-278.
Strain: 129/Sv.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF222895 mRNA. Translation: AAF37272.1.
AF207741 mRNA. Translation: AAL05976.1.
AF207742 Genomic DNA. Translation: AAL05977.1.
AK004965 mRNA. Translation: BAB23705.1.
AK004848 mRNA. Translation: BAB23614.1.
BC013654 mRNA. Translation: AAH13654.1.
AF312033 Genomic DNA. Translation: AAK28830.1.
RefSeqNP_056614.3. NM_015799.4.
XP_006504656.1. XM_006504593.1.
XP_006504657.1. XM_006504594.1.
UniGeneMm.21757.

3D structure databases

ProteinModelPortalQ9JKX3.
SMRQ9JKX3. Positions 130-798.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9JKX3. 2 interactions.
MINTMINT-1857634.

Protein family/group databases

MEROPSM28.973.

PTM databases

PhosphoSiteQ9JKX3.

Proteomic databases

PaxDbQ9JKX3.
PRIDEQ9JKX3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000031729; ENSMUSP00000031729; ENSMUSG00000029716. [Q9JKX3-1]
GeneID50765.
KEGGmmu:50765.
UCSCuc009acv.1. mouse. [Q9JKX3-1]

Organism-specific databases

CTD7036.
MGIMGI:1354956. Tfr2.

Phylogenomic databases

eggNOGNOG74799.
GeneTreeENSGT00550000074421.
HOGENOMHOG000124067.
HOVERGENHBG023177.
InParanoidQ9JKX3.
OMALFISWDG.
OrthoDBEOG780RKW.
TreeFamTF312981.

Gene expression databases

BgeeQ9JKX3.
CleanExMM_TRFR2.
GenevestigatorQ9JKX3.

Family and domain databases

Gene3D1.20.930.40. 1 hit.
InterProIPR007484. Peptidase_M28.
IPR003137. Protease-assoc_domain.
IPR007365. TFR-like_dimer_dom.
[Graphical view]
PfamPF02225. PA. 1 hit.
PF04389. Peptidase_M28. 1 hit.
PF04253. TFR_dimer. 1 hit.
[Graphical view]
SUPFAMSSF47672. SSF47672. 1 hit.
ProtoNetSearch...

Other

NextBio307667.
PROQ9JKX3.
SOURCESearch...

Entry information

Entry nameTFR2_MOUSE
AccessionPrimary (citable) accession number: Q9JKX3
Secondary accession number(s): Q920I6, Q99MQ9, Q9CPT2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: February 11, 2002
Last modified: March 19, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot