Reviewed, UniProtKB/Swiss-Prot Q9JKV7 (EXTL1_MOUSE)

Names and origin

Protein names

Recommended name:
    Exostosin-like 1
    EC=2.4.1.224
Alternative name(s):
    Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
    Exostosin-L
    Multiple exostosis-like protein

Gene names

Name:

Extl1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	669 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable glycosyltransferase By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 47 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro
Molecular function	glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 669	669	Exostosin-like 1		PRO_0000149654
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 29	21	Signal-anchor for type II membrane protein Potential
	Topological domain	30 – 669	640	Lumenal Potential
Amino acid modifications
	Glycosylation	263	1	N-linked (GlcNAc...) Potential
	Glycosylation	480	1	N-linked (GlcNAc...) Potential
	Disulfide bond	577 ↔ 627		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9JKV7-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: CD547AEF25B4E4B1	FASTA	669	74,009
	10 20 30 40 50 60 MLWRRKSFWL ALSAFWLLLV LLGVFPLRLA VSPGPLPGRS QGWPRWLDAA FLQSFSQSET 70 80 90 100 110 120 NPEDVAQLPR VSRGSSCTWG ACFDTSKCRG KVLKIFVHSP AGPTSEAQRR ILDSLEGSRY 130 140 150 160 170 180 SALSPADACL LLFLPSQDRR GACGPLPPNW NGGRNHLVLS LYPAPCTRLG QAMVAEASPS 190 200 210 220 230 240 SDIFRPGFDI ALPYLPEAHP LRGGAPGKLQ QHSPQPGATL LAVAEEKGRW RITSTHASAC 250 260 270 280 290 300 LWDRHCEQDP GPQQTYPGET LPNATFCLIP GHRSATSCFL QALQAGCIPV LLSPRWELPF 310 320 330 340 350 360 SEVIDWTKAA IIADERLPLQ VLAALREMLP SRVLALRQQT QFLWTAYFSS VEKVIHTTLE 370 380 390 400 410 420 VIQDRIWGAS GHPSLMWNSP PGALLALPTF STSLQDFPFY HLQLGSGPGS SFSAVIWVGA 430 440 450 460 470 480 SGESLLKLIQ EVAGSRHCAQ ILILWNSEKP PPDRWPETAV PLTVIKGHRK VSNRFFPYSN 490 500 510 520 530 540 ISTNVILSLD AQSTLSTSEV DFAFVVWQSF PERMVGFLSG SHFWDEAQGG WGYRTGMTND 550 560 570 580 590 600 FSMVLTTAAF YHRYYHTLFT HSLPKALRTI ADETPTCVDV LMNFLVATVT KLPPIKVPYG 610 620 630 640 650 660 RQHPEAVPMD SGDPRPVPEP QPLDQDCINR LAAGFGHMPL VSSQVRLDPV LFKDPVSVQR KKYRSLEKP

References

[1]	"EXT genes are differentially expressed in bone and cartilage during mouse embryogenesis." Stickens D., Brown D., Evans G.A. Dev. Dyn. 218:452-464(2000) [PubMed: 10878610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal brain.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

EXTL1 (putative HS transferase) [GAG Enzyme]

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF224461 mRNA. Translation: AAF61913.1.
IPI	IPI00123491.
UniGene	Mm.30978
3D structure databases
HSSP	HSSP built from PDB template 1OMX based on UniProtKB Q9ES89.
ModBase	Search...
Protein family/group databases
CAZy	GT47. Glycosyltransferase Family 47. GT64. Glycosyltransferase Family 64.
Proteomic databases
PRIDE	Q9JKV7.
Genome annotation databases
Ensembl	ENSMUSG00000028838. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:1888742. Extl1.
Phylogenomic databases
HOGENOM	Q9JKV7.
HOVERGEN	Q9JKV7.
Enzyme and pathway databases
BRENDA	2.4.1.224. 244.
Gene expression databases
ArrayExpress	Q9JKV7.
Bgee	Q9JKV7.
CleanEx	MM_EXTL1.
GermOnline	ENSMUSG00000028838. Mus musculus.
Family and domain databases
InterPro	IPR004263. Exostosin. IPR015338. HexNAc_Trfase_a. [Graphical view]
Pfam	PF03016. Exostosin. 1 hit. PF09258. Glyco_transf_64. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

EXTL1_MOUSE

Accession

Primary (citable) accession number: Q9JKV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	October 1, 2000
Last modified:	April 14, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)