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Protein

Serine/threonine-protein kinase ICK

Gene

Ick

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Has an essential role in ciliogenesis, particularly in neuronal and retinal progenitor cells (PubMed:24797473). Phosphorylates KIF3A (PubMed:24797473). Involved in the control of ciliary length (PubMed:24853502). Regulates the ciliary localization of SHH pathway components as well as the localization of IFT components at ciliary tips (PubMed:24797473, PubMed:24853502). May play a role in cardiac development (By similarity).By similarity2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATPBy similarityPROSITE-ProRule annotation
Active sitei125 – 1251Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPBy similarityPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
  3. magnesium ion binding Source: UniProtKB
  4. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. cilium assembly Source: UniProtKB
  2. intracellular signal transduction Source: UniProtKB
  3. intraciliary transport Source: UniProtKB
  4. multicellular organismal development Source: UniProtKB-KW
  5. protein phosphorylation Source: UniProtKB
  6. signal transduction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase ICK (EC:2.7.11.22)
Alternative name(s):
Intestinal cell kinase
Short name:
mICK
MAK-related kinase
Short name:
MRK
Gene namesi
Name:Ick
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1934157. Ick.

Subcellular locationi

Cytoplasmcytosol By similarity. Cell projectioncilium 1 Publication. Nucleus By similarity. Cytoplasmcytoskeletoncilium basal body 1 Publication
Note: Also found at the ciliary tip (PubMed:24797473).By similarity2 Publications

GO - Cellular componenti

  1. ciliary basal body Source: UniProtKB
  2. ciliary tip Source: UniProtKB
  3. cytosol Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Disruption phenotypei

Knockout mice manifest hydrocephalus, polydactyly, and delayed skeletal development. At cellular levels, ICK knockout results in abnormally elongated cilia and compromised SHH signaling.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 629629Serine/threonine-protein kinase ICKPRO_0000086008Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei157 – 1571PhosphothreonineBy similarity
Modified residuei159 – 1591PhosphotyrosineBy similarity

Post-translational modificationi

Autophosphorylated on serine and threonine residues. May play a role in enzyme activation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9JKV2.
PRIDEiQ9JKV2.

PTM databases

PhosphoSiteiQ9JKV2.

Expressioni

Tissue specificityi

Highly expressed in colon and lung, lower levels present in heart, esophagus, stomach, small intestine and ovary. Localizes to the crypt region of large and small intestine.1 Publication

Gene expression databases

BgeeiQ9JKV2.
CleanExiMM_ICK.
ExpressionAtlasiQ9JKV2. baseline and differential.
GenevestigatoriQ9JKV2.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112961.

Structurei

3D structure databases

ProteinModelPortaliQ9JKV2.
SMRiQ9JKV2. Positions 1-413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 284281Protein kinaseCuratedPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00650000093283.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiQ9JKV2.
KOiK08828.
OMAiCIMAEVY.
OrthoDBiEOG7NCV37.
PhylomeDBiQ9JKV2.
TreeFamiTF328769.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JKV2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNRYTTIKQL GDGTYGSVLL GRSIESGELI AIKKMKRKFY SWEECMNLRE
60 70 80 90 100
VKSLKKLNHA NIVKLKEVIR ENDHLYFIFE YMKENLYQLI KERNKLFPES
110 120 130 140 150
AIRNIMYQIL QGLAFIHKHG FFHRDLKPEN LLCMGPELVK IADFGLAREI
160 170 180 190 200
RSRPPYTDYV STRWYRAPEV LLRSTNYSSP IDIWAVGCIM AEVYTLRPLF
210 220 230 240 250
PGASEIDTIF KICQVLGTPK KTDWPEGYQL SSAMNFLWPQ CIPNNLKTLI
260 270 280 290 300
PNASSEAIQL LRDLLQWDPK KRPTASQALR YPYFQIGHPL GIISKDSGKP
310 320 330 340 350
QREVQDKTGP PPYIKPAPPA QAPAKAYTLI SSRPSQASQP PQHSVHPYKG
360 370 380 390 400
DVSRTEQLSH VQEGKPSPPF FPSLHNKNLQ PKILASLEQK NGEIKPKSRR
410 420 430 440 450
RWGLISRSTK GSDDWADLDD LDFSPSLTRI DVKNKKRQSD DTLCRFESVL
460 470 480 490 500
DLKPSESVGT GTTVSTQASS QRRDTPTLQS SAKQHYLKHS RYLPGINIRN
510 520 530 540 550
GVLPNPGKDF LPSNSWSSSG LSGKSSGTVS VVSKITSVGS GSASSSGLTG
560 570 580 590 600
SYIPSFLKKE IGSVMQRVQL APLAAPPPGY SSLKAVRPHP GRPFFHTQPR
610 620
STPGLIPRPP AAQPVHGRID WSSKYPSRR
Length:629
Mass (Da):70,592
Last modified:June 7, 2004 - v2
Checksum:i43E863847C5FD9C5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti533 – 5331S → R in AAF37277. (PubMed:10699974)Curated
Sequence conflicti616 – 6161H → L in AAF37277. (PubMed:10699974)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF225918 mRNA. Translation: AAF37277.1.
BC028863 mRNA. Translation: AAH28863.1.
CCDSiCCDS40697.1.
RefSeqiNP_001157252.1. NM_001163780.1.
NP_064371.2. NM_019987.2.
XP_006511367.1. XM_006511304.1.
UniGeneiMm.288719.

Genome annotation databases

EnsembliENSMUST00000044551; ENSMUSP00000048234; ENSMUSG00000009828.
ENSMUST00000118869; ENSMUSP00000112961; ENSMUSG00000009828.
GeneIDi56542.
KEGGimmu:56542.
UCSCiuc009qtt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF225918 mRNA. Translation: AAF37277.1.
BC028863 mRNA. Translation: AAH28863.1.
CCDSiCCDS40697.1.
RefSeqiNP_001157252.1. NM_001163780.1.
NP_064371.2. NM_019987.2.
XP_006511367.1. XM_006511304.1.
UniGeneiMm.288719.

3D structure databases

ProteinModelPortaliQ9JKV2.
SMRiQ9JKV2. Positions 1-413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112961.

PTM databases

PhosphoSiteiQ9JKV2.

Proteomic databases

PaxDbiQ9JKV2.
PRIDEiQ9JKV2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044551; ENSMUSP00000048234; ENSMUSG00000009828.
ENSMUST00000118869; ENSMUSP00000112961; ENSMUSG00000009828.
GeneIDi56542.
KEGGimmu:56542.
UCSCiuc009qtt.2. mouse.

Organism-specific databases

CTDi22858.
MGIiMGI:1934157. Ick.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00650000093283.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiQ9JKV2.
KOiK08828.
OMAiCIMAEVY.
OrthoDBiEOG7NCV37.
PhylomeDBiQ9JKV2.
TreeFamiTF328769.

Miscellaneous databases

NextBioi312910.
PROiQ9JKV2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JKV2.
CleanExiMM_ICK.
ExpressionAtlasiQ9JKV2. baseline and differential.
GenevestigatoriQ9JKV2.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Intestinal cell kinase (ICK) localizes to the crypt region and requires a dual phosphorylation site found in map kinases."
    Togawa K., Yan Y.-X., Inomoto T., Slaugenhaupt S.A., Rustgi A.K.
    J. Cell. Physiol. 183:129-139(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Ileum1 Publication and Jejunum1 Publication.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: ColonImported.
  3. "ICK is essential for cell type-specific ciliogenesis and the regulation of ciliary transport."
    Chaya T., Omori Y., Kuwahara R., Furukawa T.
    EMBO J. 33:1227-1242(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. "Intestinal cell kinase, a protein associated with endocrine-cerebro-osteodysplasia syndrome, is a key regulator of cilia length and Hedgehog signaling."
    Moon H., Song J., Shin J.O., Lee H., Kim H.K., Eggenschwiller J.T., Bok J., Ko H.W.
    Proc. Natl. Acad. Sci. U.S.A. 111:8541-8546(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiICK_MOUSE
AccessioniPrimary (citable) accession number: Q9JKV2
Secondary accession number(s): Q8K138
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: February 4, 2015
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.