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Protein

Iroquois-class homeodomain protein IRX-5

Gene

Irx5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Establishes the cardiac repolarization gradient by its repressive actions on the KCND2 potassium-channel gene. Required for retinal cone bipolar cell differentiation. May regulate contrast adaptation in the retina and control specific aspects of visual function in circuits of the mammalian retina. Involved in craniofacial and gonadal development (By similarity). Modulates the migration of progenitor cell populations in branchial arches and gonads by repressing CXCL12.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi112 – 17463Homeobox; TALE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • cell development Source: MGI
  • embryonic cranial skeleton morphogenesis Source: Ensembl
  • gonad development Source: Ensembl
  • neuron maturation Source: MGI
  • regulation of gene expression Source: MGI
  • regulation of heart rate Source: MGI
  • regulation of transcription, DNA-templated Source: InterPro
  • response to stimulus Source: UniProtKB-KW
  • retinal bipolar neuron differentiation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Sensory transduction, Transcription, Vision

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Iroquois-class homeodomain protein IRX-5
Alternative name(s):
Homeodomain protein IRXB2
Iroquois homeobox protein 5
Gene namesi
Name:Irx5
Synonyms:Irxb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1859086. Irx5.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Disruption causes increased KCND2 potassium-channel expression in endocardial myocardium leading to abolition of the cardiac repolarization gradient, a selective increase of the major cardiac repolarization current, I(to,f), and increased susceptibility to arrhythmias.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484Iroquois-class homeodomain protein IRX-5PRO_0000049161Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei273 – 2731PhosphoserineBy similarity
Modified residuei465 – 4651PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9JKQ4.
PRIDEiQ9JKQ4.

PTM databases

iPTMnetiQ9JKQ4.
PhosphoSiteiQ9JKQ4.

Expressioni

Tissue specificityi

Not expressed in the developing metanephric kidney or adult kidney.

Developmental stagei

In E9.5 embryos, expressed in the rhombencephalon, metencephalon, and in the cephalic mesoderm surround the optic vesicle. By E12.5, expression continues in the mesenchyme and also begins in subsets of cells in the neuroretina, becoming expressed in the retinal inner neuroblast layers by E16.5. Expressed in developing bipolar cells during retinal development starting at postnatal day 5, and expressed in a subset of cone bipolar cells in the mature retina. Also expressed along the spinal cord, in the ventricular layer, in motor neurons and in the proximal limb buds during embryonic development. Expressed in the developing heart in the endocardium that lines the heart chambers.3 Publications

Gene expression databases

BgeeiQ9JKQ4.
CleanExiMM_IRX5.
GenevisibleiQ9JKQ4. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034184.

Structurei

3D structure databases

ProteinModelPortaliQ9JKQ4.
SMRiQ9JKQ4. Positions 123-170.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi72 – 787Poly-Ala
Compositional biasi185 – 1917Poly-Glu
Compositional biasi319 – 3279Poly-Pro
Compositional biasi351 – 3544Poly-Gly

Sequence similaritiesi

Belongs to the TALE/IRO homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0773. Eukaryota.
ENOG410XPMQ. LUCA.
GeneTreeiENSGT00750000117365.
HOGENOMiHOG000234546.
HOVERGENiHBG006180.
InParanoidiQ9JKQ4.
OMAiFGHLHSN.
OrthoDBiEOG7TJ3HS.
PhylomeDBiQ9JKQ4.
TreeFamiTF319371.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
IPR003893. Iroquois_homeo.
[Graphical view]
PfamiPF05920. Homeobox_KN. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00548. IRO. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JKQ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYPQGYLYQ PSASLALYSC PAYSTSVISG PRTDELGRSS SGSAFSPYAG
60 70 80 90 100
STAFTAPSPG YNSHLQYGAD PAAAAAAAFS YVGSPYDHTP GMAGSLGYHP
110 120 130 140 150
YAAPLGSYPY GDPAYRKNAT RDATATLKAW LNEHRKNPYP TKGEKIMLAI
160 170 180 190 200
ITKMTLTQVS TWFANARRRL KKENKMTWTP RNRSEDEEEE ENIDLEKNDE
210 220 230 240 250
DEPQKPEDKG DLEGPESGGA EQKATAGCER LQGPLSPAGK ETEGSLSDSD
260 270 280 290 300
FKESSSEGRH DELPRPPRAG ESSPAGPATA RLAEDAGPHY PASVPAPGPH
310 320 330 340 350
PSAGELPPGS GGSSVIHSPP PPPPPPPAVL AKPKLWSLAE IATSSDKVKD
360 370 380 390 400
GGGGSEGSPC PPCPGPMGGQ TLGGSRASPA PAPARSPSAQ CPFPGGTVLS
410 420 430 440 450
RPLYYTAPFY PGYTNYGSFG HLHGHPGPGP SPTAGPGSHF NGLNQTVLNR
460 470 480
ADVLAKDPKM LRSQSQLDLC KDSPYELKKG MSDI
Length:484
Mass (Da):50,755
Last modified:October 1, 2000 - v1
Checksum:i4C9AB3ED9992EDC9
GO

Sequence cautioni

The sequence AAF63955.1 differs from that shown. Reason: Frameshift at position 430. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF230074 mRNA. Translation: AAF42871.1.
AK004747 mRNA. Translation: BAB23528.1.
BC051959 mRNA. Translation: AAH51959.2.
BC056994 mRNA. Translation: AAH56994.1.
AF165985 mRNA. Translation: AAF63955.1. Frameshift.
CCDSiCCDS22522.1.
RefSeqiNP_061296.1. NM_018826.2.
UniGeneiMm.101153.

Genome annotation databases

EnsembliENSMUST00000034184; ENSMUSP00000034184; ENSMUSG00000031737.
GeneIDi54352.
KEGGimmu:54352.
UCSCiuc009mub.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF230074 mRNA. Translation: AAF42871.1.
AK004747 mRNA. Translation: BAB23528.1.
BC051959 mRNA. Translation: AAH51959.2.
BC056994 mRNA. Translation: AAH56994.1.
AF165985 mRNA. Translation: AAF63955.1. Frameshift.
CCDSiCCDS22522.1.
RefSeqiNP_061296.1. NM_018826.2.
UniGeneiMm.101153.

3D structure databases

ProteinModelPortaliQ9JKQ4.
SMRiQ9JKQ4. Positions 123-170.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034184.

PTM databases

iPTMnetiQ9JKQ4.
PhosphoSiteiQ9JKQ4.

Proteomic databases

PaxDbiQ9JKQ4.
PRIDEiQ9JKQ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034184; ENSMUSP00000034184; ENSMUSG00000031737.
GeneIDi54352.
KEGGimmu:54352.
UCSCiuc009mub.1. mouse.

Organism-specific databases

CTDi10265.
MGIiMGI:1859086. Irx5.

Phylogenomic databases

eggNOGiKOG0773. Eukaryota.
ENOG410XPMQ. LUCA.
GeneTreeiENSGT00750000117365.
HOGENOMiHOG000234546.
HOVERGENiHBG006180.
InParanoidiQ9JKQ4.
OMAiFGHLHSN.
OrthoDBiEOG7TJ3HS.
PhylomeDBiQ9JKQ4.
TreeFamiTF319371.

Miscellaneous databases

PROiQ9JKQ4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JKQ4.
CleanExiMM_IRX5.
GenevisibleiQ9JKQ4. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
IPR003893. Iroquois_homeo.
[Graphical view]
PfamiPF05920. Homeobox_KN. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00548. IRO. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Patterning the embryonic heart: identification of five mouse Iroquois homeobox genes in the developing heart."
    Christoffels V.M., Keijser A.G.M., Houweling A.C., Clout D.E.W., Moorman A.F.M.
    Dev. Biol. 224:263-274(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Strain: FVB/N.
    Tissue: Embryonic heart.
  2. "Identification of a novel mouse Iroquois homeobox gene, Irx5, and chromosomal localisation of all members of the mouse Iroquois gene family."
    Bosse A., Stoykova A., Nieselt-Struwe K., Chowdhury K., Copeland N.G., Jenkins N.A., Gruss P.
    Dev. Dyn. 218:160-174(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Lung.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  5. "Expression of two novel mouse Iroquois-class homeobox genes during neurogenesis."
    Cohen D.R., Cheng C.W., Cheng S.H., Hui C.-C.
    Mech. Dev. 91:317-321(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-442, DEVELOPMENTAL STAGE.
    Tissue: Brain.
  6. Cited for: DISRUPTION PHENOTYPE, POSSIBLE FUNCTION.
  7. "The Iroquois homeobox gene, Irx5, is required for retinal cone bipolar cell development."
    Cheng C.W., Chow R.L., Lebel M., Sakuma R., Cheung H.O.-L., Thanabalasingham V., Zhang X., Bruneau B.G., Birch D.G., Hui C.C., McInnes R.R., Cheng S.H.
    Dev. Biol. 287:48-60(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE FUNCTION, DEVELOPMENTAL STAGE.
  8. "The prepattern transcription factor Irx3 directs nephron segment identity."
    Reggiani L., Raciti D., Airik R., Kispert A., Braendli A.W.
    Genes Dev. 21:2358-2370(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF RENAL EXPRESSION.
  9. "Genetic control of circuit function: Vsx1 and Irx5 transcription factors regulate contrast adaptation in the mouse retina."
    Kerschensteiner D., Liu H., Cheng C.W., Demas J., Cheng S.H., Hui C.C., Chow R.L., Wong R.O.L.
    J. Neurosci. 28:2342-2352(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE FUNCTION.

Entry informationi

Entry nameiIRX5_MOUSE
AccessioniPrimary (citable) accession number: Q9JKQ4
Secondary accession number(s): Q80WV4, Q9JLL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: October 1, 2000
Last modified: July 6, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.