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Q9JKQ2

- S26A5_MERUN

UniProt

Q9JKQ2 - S26A5_MERUN

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Protein

Prestin

Gene

SLC26A5

Organism
Meriones unguiculatus (Mongolian jird) (Mongolian gerbil)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site (By similarity).By similarity

GO - Molecular functioni

  1. secondary active sulfate transmembrane transporter activity Source: InterPro

GO - Biological processi

  1. regulation of cell shape Source: UniProtKB-KW
  2. sensory perception of sound Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell shape, Hearing

Names & Taxonomyi

Protein namesi
Recommended name:
Prestin
Alternative name(s):
Solute carrier family 26 member 5
Gene namesi
Name:SLC26A5
Synonyms:PRES
OrganismiMeriones unguiculatus (Mongolian jird) (Mongolian gerbil)
Taxonomic identifieri10047 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeGerbillinaeMeriones

Subcellular locationi

Basolateral cell membrane; Multi-pass membrane protein
Note: Basolateral wall of outer hair cells.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7979CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei80 – 10021Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini101 – 1022ExtracellularSequence Analysis
Transmembranei103 – 12321Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini124 – 1318CytoplasmicSequence Analysis
Transmembranei132 – 15221Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini153 – 18331ExtracellularSequence AnalysisAdd
BLAST
Transmembranei184 – 20421Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini205 – 2117CytoplasmicSequence Analysis
Transmembranei212 – 23221Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini233 – 25321ExtracellularSequence AnalysisAdd
BLAST
Transmembranei254 – 27421Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini275 – 28612CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei287 – 30721Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini308 – 33427ExtracellularSequence AnalysisAdd
BLAST
Transmembranei335 – 35521Helical; Name=8Sequence AnalysisAdd
BLAST
Topological domaini356 – 37419CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei375 – 39521Helical; Name=9Sequence AnalysisAdd
BLAST
Topological domaini396 – 41116ExtracellularSequence AnalysisAdd
BLAST
Transmembranei412 – 43221Helical; Name=10Sequence AnalysisAdd
BLAST
Topological domaini433 – 4419CytoplasmicSequence Analysis
Transmembranei442 – 46221Helical; Name=11Sequence AnalysisAdd
BLAST
Topological domaini463 – 47917ExtracellularSequence AnalysisAdd
BLAST
Transmembranei480 – 50021Helical; Name=12Sequence AnalysisAdd
BLAST
Topological domaini501 – 744244CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 744744PrestinPRO_0000080168Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Highly expressed in mature outer hair cells, but not in inner hair cells or other cells of the basilar membrane and the organ of Corti.

Developmental stagei

Detected in the organ of Corti as early as 6 days after birth; levels increase up to day 20, concomitant with the development of high sensitivity hearing.

Structurei

3D structure databases

ProteinModelPortaliQ9JKQ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini525 – 713189STASPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 STAS domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG000639.

Family and domain databases

Gene3Di3.30.750.24. 2 hits.
InterProiIPR018045. S04_transporter_CS.
IPR002645. STAS_dom.
IPR001902. SulP_transpt.
IPR011547. Sulph_transpt.
[Graphical view]
PfamiPF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view]
SUPFAMiSSF52091. SSF52091. 2 hits.
TIGRFAMsiTIGR00815. sulP. 1 hit.
PROSITEiPS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JKQ2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDHAEENEIP VATQKYHVER PIFSHPVLQE RLHVKDKVSE SIGDKLKQAF
60 70 80 90 100
TCTPKKIRNI IYMFLPITKW LPAYKFKEYV LGDLVSGIST GVLQLPQGLA
110 120 130 140 150
FAMLAAVPPV FGLYSSFYPV IMYCFFGTSR HISIGPFAVI SLMIGGVAVR
160 170 180 190 200
LVPDDIVIPG GVNATNGTEA RDALRVKVAM SVTLLSGIIQ FCLGVCRFGF
210 220 230 240 250
VAIYLTEPLV RGFTTAAAVH VFTSMLKYLF GVKTKRYSGI FSVVYSTVAV
260 270 280 290 300
LQNVKNLNVC SLGVGLMVFG LLLGGKEFNE RFKEKLPAPI PLEFFAVVMG
310 320 330 340 350
TGISAGFNLH ESYSVDVVGT LPLGLLPPAN PDTSLFHLVY VDAIAIAIVG
360 370 380 390 400
FSVTISMAKT LANKHGYQVD GNQELIALGI CNSIGSLFQT FSISCSLSRS
410 420 430 440 450
LVQEGTGGKT QLAGCLASLM ILLVILATGF LFESLPQAVL SAIVIVNLKG
460 470 480 490 500
MFMQFSDLPF FWRTSKIELT IWLTTFVSSL FLGLDYGLIT AVIIALLTVI
510 520 530 540 550
YRTQSPSYKV LGQLPDTDVY IDIDAYEEVK EIPGIKIFQI NAPIYYANSD
560 570 580 590 600
LYSNALKRKT GVNPALIMGA RRKAMRKYAK EVGNANIANA AVVKVDGEVD
610 620 630 640 650
GENATKPEEE DDEVKYPPIV IKTTFPEELQ RFMPQTENVH TIILDFTQVN
660 670 680 690 700
FIDSVGVKTL AVMVKEYGDV GIYVYLAGCS PQVVNDLTRN RFFENPALKE
710 720 730 740
LLFHSIHDAV LGSHVREAMA EQEASAPPPQ DDMEPNATPT TPEA
Length:744
Mass (Da):81,419
Last modified:October 1, 2000 - v1
Checksum:i1CDF66589DAACBBC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF230376 mRNA. Translation: AAF71715.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

Pump up the volume - Issue 22 of May 2002

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF230376 mRNA. Translation: AAF71715.1 .

3D structure databases

ProteinModelPortali Q9JKQ2.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG000639.

Family and domain databases

Gene3Di 3.30.750.24. 2 hits.
InterProi IPR018045. S04_transporter_CS.
IPR002645. STAS_dom.
IPR001902. SulP_transpt.
IPR011547. Sulph_transpt.
[Graphical view ]
Pfami PF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view ]
SUPFAMi SSF52091. SSF52091. 2 hits.
TIGRFAMsi TIGR00815. sulP. 1 hit.
PROSITEi PS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Prestin is the motor protein of cochlear outer hair cells."
    Zheng J., Shen W., He D.Z.Z., Long K.B., Madison L.D., Dallos P.
    Nature 405:149-155(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Organ of Corti.
  2. "Prestin topology: localization of protein epitopes in relation to the plasma membrane."
    Zheng J., Long K.B., Shen W., Madison L.D., Dallos P.
    NeuroReport 12:1929-1935(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.

Entry informationi

Entry nameiS26A5_MERUN
AccessioniPrimary (citable) accession number: Q9JKQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3