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Q9JKQ2

- S26A5_MERUN

UniProt

Q9JKQ2 - S26A5_MERUN

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Protein
Prestin
Gene
SLC26A5, PRES
Organism
Meriones unguiculatus (Mongolian jird) (Mongolian gerbil)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site By similarity.

GO - Molecular functioni

  1. secondary active sulfate transmembrane transporter activity Source: InterPro

GO - Biological processi

  1. regulation of cell shape Source: UniProtKB-KW
  2. sensory perception of sound Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell shape, Hearing

Names & Taxonomyi

Protein namesi
Recommended name:
Prestin
Alternative name(s):
Solute carrier family 26 member 5
Gene namesi
Name:SLC26A5
Synonyms:PRES
OrganismiMeriones unguiculatus (Mongolian jird) (Mongolian gerbil)
Taxonomic identifieri10047 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeGerbillinaeMeriones

Subcellular locationi

Basolateral cell membrane; Multi-pass membrane protein
Note: Basolateral wall of outer hair cells.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7979Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei80 – 10021Helical; Name=1; Reviewed prediction
Add
BLAST
Topological domaini101 – 1022Extracellular Reviewed prediction
Transmembranei103 – 12321Helical; Name=2; Reviewed prediction
Add
BLAST
Topological domaini124 – 1318Cytoplasmic Reviewed prediction
Transmembranei132 – 15221Helical; Name=3; Reviewed prediction
Add
BLAST
Topological domaini153 – 18331Extracellular Reviewed prediction
Add
BLAST
Transmembranei184 – 20421Helical; Name=4; Reviewed prediction
Add
BLAST
Topological domaini205 – 2117Cytoplasmic Reviewed prediction
Transmembranei212 – 23221Helical; Name=5; Reviewed prediction
Add
BLAST
Topological domaini233 – 25321Extracellular Reviewed prediction
Add
BLAST
Transmembranei254 – 27421Helical; Name=6; Reviewed prediction
Add
BLAST
Topological domaini275 – 28612Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei287 – 30721Helical; Name=7; Reviewed prediction
Add
BLAST
Topological domaini308 – 33427Extracellular Reviewed prediction
Add
BLAST
Transmembranei335 – 35521Helical; Name=8; Reviewed prediction
Add
BLAST
Topological domaini356 – 37419Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei375 – 39521Helical; Name=9; Reviewed prediction
Add
BLAST
Topological domaini396 – 41116Extracellular Reviewed prediction
Add
BLAST
Transmembranei412 – 43221Helical; Name=10; Reviewed prediction
Add
BLAST
Topological domaini433 – 4419Cytoplasmic Reviewed prediction
Transmembranei442 – 46221Helical; Name=11; Reviewed prediction
Add
BLAST
Topological domaini463 – 47917Extracellular Reviewed prediction
Add
BLAST
Transmembranei480 – 50021Helical; Name=12; Reviewed prediction
Add
BLAST
Topological domaini501 – 744244Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 744744Prestin
PRO_0000080168Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi163 – 1631N-linked (GlcNAc...) Reviewed prediction
Glycosylationi166 – 1661N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Highly expressed in mature outer hair cells, but not in inner hair cells or other cells of the basilar membrane and the organ of Corti.

Developmental stagei

Detected in the organ of Corti as early as 6 days after birth; levels increase up to day 20, concomitant with the development of high sensitivity hearing.

Structurei

3D structure databases

ProteinModelPortaliQ9JKQ2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini525 – 713189STAS
Add
BLAST

Sequence similaritiesi

Contains 1 STAS domain.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG000639.

Family and domain databases

Gene3Di3.30.750.24. 2 hits.
InterProiIPR018045. S04_transporter_CS.
IPR002645. STAS_dom.
IPR001902. SulP_transpt.
IPR011547. Sulph_transpt.
[Graphical view]
PfamiPF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view]
SUPFAMiSSF52091. SSF52091. 2 hits.
TIGRFAMsiTIGR00815. sulP. 1 hit.
PROSITEiPS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JKQ2-1 [UniParc]FASTAAdd to Basket

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MDHAEENEIP VATQKYHVER PIFSHPVLQE RLHVKDKVSE SIGDKLKQAF    50
TCTPKKIRNI IYMFLPITKW LPAYKFKEYV LGDLVSGIST GVLQLPQGLA 100
FAMLAAVPPV FGLYSSFYPV IMYCFFGTSR HISIGPFAVI SLMIGGVAVR 150
LVPDDIVIPG GVNATNGTEA RDALRVKVAM SVTLLSGIIQ FCLGVCRFGF 200
VAIYLTEPLV RGFTTAAAVH VFTSMLKYLF GVKTKRYSGI FSVVYSTVAV 250
LQNVKNLNVC SLGVGLMVFG LLLGGKEFNE RFKEKLPAPI PLEFFAVVMG 300
TGISAGFNLH ESYSVDVVGT LPLGLLPPAN PDTSLFHLVY VDAIAIAIVG 350
FSVTISMAKT LANKHGYQVD GNQELIALGI CNSIGSLFQT FSISCSLSRS 400
LVQEGTGGKT QLAGCLASLM ILLVILATGF LFESLPQAVL SAIVIVNLKG 450
MFMQFSDLPF FWRTSKIELT IWLTTFVSSL FLGLDYGLIT AVIIALLTVI 500
YRTQSPSYKV LGQLPDTDVY IDIDAYEEVK EIPGIKIFQI NAPIYYANSD 550
LYSNALKRKT GVNPALIMGA RRKAMRKYAK EVGNANIANA AVVKVDGEVD 600
GENATKPEEE DDEVKYPPIV IKTTFPEELQ RFMPQTENVH TIILDFTQVN 650
FIDSVGVKTL AVMVKEYGDV GIYVYLAGCS PQVVNDLTRN RFFENPALKE 700
LLFHSIHDAV LGSHVREAMA EQEASAPPPQ DDMEPNATPT TPEA 744
Length:744
Mass (Da):81,419
Last modified:October 1, 2000 - v1
Checksum:i1CDF66589DAACBBC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF230376 mRNA. Translation: AAF71715.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

Pump up the volume - Issue 22 of May 2002

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF230376 mRNA. Translation: AAF71715.1 .

3D structure databases

ProteinModelPortali Q9JKQ2.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG000639.

Family and domain databases

Gene3Di 3.30.750.24. 2 hits.
InterProi IPR018045. S04_transporter_CS.
IPR002645. STAS_dom.
IPR001902. SulP_transpt.
IPR011547. Sulph_transpt.
[Graphical view ]
Pfami PF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view ]
SUPFAMi SSF52091. SSF52091. 2 hits.
TIGRFAMsi TIGR00815. sulP. 1 hit.
PROSITEi PS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Prestin is the motor protein of cochlear outer hair cells."
    Zheng J., Shen W., He D.Z.Z., Long K.B., Madison L.D., Dallos P.
    Nature 405:149-155(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Organ of Corti.
  2. "Prestin topology: localization of protein epitopes in relation to the plasma membrane."
    Zheng J., Long K.B., Shen W., Madison L.D., Dallos P.
    NeuroReport 12:1929-1935(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.

Entry informationi

Entry nameiS26A5_MERUN
AccessioniPrimary (citable) accession number: Q9JKQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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