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Q9JKQ2

- S26A5_MERUN

UniProt

Q9JKQ2 - S26A5_MERUN

Protein

Prestin

Gene

SLC26A5

Organism
Meriones unguiculatus (Mongolian jird) (Mongolian gerbil)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site By similarity.By similarity

    GO - Molecular functioni

    1. secondary active sulfate transmembrane transporter activity Source: InterPro

    GO - Biological processi

    1. regulation of cell shape Source: UniProtKB-KW
    2. sensory perception of sound Source: UniProtKB-KW

    Keywords - Molecular functioni

    Motor protein

    Keywords - Biological processi

    Cell shape, Hearing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prestin
    Alternative name(s):
    Solute carrier family 26 member 5
    Gene namesi
    Name:SLC26A5
    Synonyms:PRES
    OrganismiMeriones unguiculatus (Mongolian jird) (Mongolian gerbil)
    Taxonomic identifieri10047 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeGerbillinaeMeriones

    Subcellular locationi

    Basolateral cell membrane; Multi-pass membrane protein
    Note: Basolateral wall of outer hair cells.

    GO - Cellular componenti

    1. basolateral plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 744744PrestinPRO_0000080168Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    Highly expressed in mature outer hair cells, but not in inner hair cells or other cells of the basilar membrane and the organ of Corti.

    Developmental stagei

    Detected in the organ of Corti as early as 6 days after birth; levels increase up to day 20, concomitant with the development of high sensitivity hearing.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JKQ2.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 7979CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini101 – 1022ExtracellularSequence Analysis
    Topological domaini124 – 1318CytoplasmicSequence Analysis
    Topological domaini153 – 18331ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini205 – 2117CytoplasmicSequence Analysis
    Topological domaini233 – 25321ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini275 – 28612CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini308 – 33427ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini356 – 37419CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini396 – 41116ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini433 – 4419CytoplasmicSequence Analysis
    Topological domaini463 – 47917ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini501 – 744244CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei80 – 10021Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei103 – 12321Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei132 – 15221Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei184 – 20421Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei212 – 23221Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei254 – 27421Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei287 – 30721Helical; Name=7Sequence AnalysisAdd
    BLAST
    Transmembranei335 – 35521Helical; Name=8Sequence AnalysisAdd
    BLAST
    Transmembranei375 – 39521Helical; Name=9Sequence AnalysisAdd
    BLAST
    Transmembranei412 – 43221Helical; Name=10Sequence AnalysisAdd
    BLAST
    Transmembranei442 – 46221Helical; Name=11Sequence AnalysisAdd
    BLAST
    Transmembranei480 – 50021Helical; Name=12Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini525 – 713189STASPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 STAS domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOVERGENiHBG000639.

    Family and domain databases

    Gene3Di3.30.750.24. 2 hits.
    InterProiIPR018045. S04_transporter_CS.
    IPR002645. STAS_dom.
    IPR001902. SulP_transpt.
    IPR011547. Sulph_transpt.
    [Graphical view]
    PfamiPF01740. STAS. 1 hit.
    PF00916. Sulfate_transp. 1 hit.
    [Graphical view]
    SUPFAMiSSF52091. SSF52091. 2 hits.
    TIGRFAMsiTIGR00815. sulP. 1 hit.
    PROSITEiPS01130. SLC26A. 1 hit.
    PS50801. STAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9JKQ2-1 [UniParc]FASTAAdd to Basket

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    MDHAEENEIP VATQKYHVER PIFSHPVLQE RLHVKDKVSE SIGDKLKQAF    50
    TCTPKKIRNI IYMFLPITKW LPAYKFKEYV LGDLVSGIST GVLQLPQGLA 100
    FAMLAAVPPV FGLYSSFYPV IMYCFFGTSR HISIGPFAVI SLMIGGVAVR 150
    LVPDDIVIPG GVNATNGTEA RDALRVKVAM SVTLLSGIIQ FCLGVCRFGF 200
    VAIYLTEPLV RGFTTAAAVH VFTSMLKYLF GVKTKRYSGI FSVVYSTVAV 250
    LQNVKNLNVC SLGVGLMVFG LLLGGKEFNE RFKEKLPAPI PLEFFAVVMG 300
    TGISAGFNLH ESYSVDVVGT LPLGLLPPAN PDTSLFHLVY VDAIAIAIVG 350
    FSVTISMAKT LANKHGYQVD GNQELIALGI CNSIGSLFQT FSISCSLSRS 400
    LVQEGTGGKT QLAGCLASLM ILLVILATGF LFESLPQAVL SAIVIVNLKG 450
    MFMQFSDLPF FWRTSKIELT IWLTTFVSSL FLGLDYGLIT AVIIALLTVI 500
    YRTQSPSYKV LGQLPDTDVY IDIDAYEEVK EIPGIKIFQI NAPIYYANSD 550
    LYSNALKRKT GVNPALIMGA RRKAMRKYAK EVGNANIANA AVVKVDGEVD 600
    GENATKPEEE DDEVKYPPIV IKTTFPEELQ RFMPQTENVH TIILDFTQVN 650
    FIDSVGVKTL AVMVKEYGDV GIYVYLAGCS PQVVNDLTRN RFFENPALKE 700
    LLFHSIHDAV LGSHVREAMA EQEASAPPPQ DDMEPNATPT TPEA 744
    Length:744
    Mass (Da):81,419
    Last modified:October 1, 2000 - v1
    Checksum:i1CDF66589DAACBBC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF230376 mRNA. Translation: AAF71715.1.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Pump up the volume - Issue 22 of May 2002

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF230376 mRNA. Translation: AAF71715.1 .

    3D structure databases

    ProteinModelPortali Q9JKQ2.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG000639.

    Family and domain databases

    Gene3Di 3.30.750.24. 2 hits.
    InterProi IPR018045. S04_transporter_CS.
    IPR002645. STAS_dom.
    IPR001902. SulP_transpt.
    IPR011547. Sulph_transpt.
    [Graphical view ]
    Pfami PF01740. STAS. 1 hit.
    PF00916. Sulfate_transp. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52091. SSF52091. 2 hits.
    TIGRFAMsi TIGR00815. sulP. 1 hit.
    PROSITEi PS01130. SLC26A. 1 hit.
    PS50801. STAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prestin is the motor protein of cochlear outer hair cells."
      Zheng J., Shen W., He D.Z.Z., Long K.B., Madison L.D., Dallos P.
      Nature 405:149-155(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Organ of Corti.
    2. "Prestin topology: localization of protein epitopes in relation to the plasma membrane."
      Zheng J., Long K.B., Shen W., Madison L.D., Dallos P.
      NeuroReport 12:1929-1935(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY.

    Entry informationi

    Entry nameiS26A5_MERUN
    AccessioniPrimary (citable) accession number: Q9JKQ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 27, 2002
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3