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Protein

DNA polymerase epsilon subunit 3

Gene

Pole3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon subunit 3 (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit 3
DNA polymerase epsilon subunit p17
NF-YB-like protein
YB-like protein 1
Short name:
YBL1
Gene namesi
Name:Pole3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1933378. Pole3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002083422 – 145DNA polymerase epsilon subunit 3Add BLAST144

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei83PhosphothreonineBy similarity1
Modified residuei121PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9JKP7.
MaxQBiQ9JKP7.
PaxDbiQ9JKP7.
PeptideAtlasiQ9JKP7.
PRIDEiQ9JKP7.

PTM databases

iPTMnetiQ9JKP7.
PhosphoSitePlusiQ9JKP7.

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

BgeeiENSMUSG00000028394.

Interactioni

Subunit structurei

Component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4. Interaction with POLE4 is a prerequisite for further binding with POLE and POLE2. Interacts with CHRAC1. Together with CHRAC1, ACF1 and ISWI/SNF2H proteins, it forms the ISWI chromatin-remodeling complex, CHRAC (By similarity).By similarity

Protein-protein interaction databases

BioGridi208472. 3 interactors.
IntActiQ9JKP7. 1 interactor.
STRINGi10090.ENSMUSP00000030091.

Structurei

3D structure databases

ProteinModelPortaliQ9JKP7.
SMRiQ9JKP7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili85 – 144Sequence analysisAdd BLAST60

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0870. Eukaryota.
ENOG4111QSW. LUCA.
HOGENOMiHOG000007701.
HOVERGENiHBG060262.
InParanoidiQ9JKP7.
KOiK02326.
PhylomeDBiQ9JKP7.
TreeFamiTF103008.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
[Graphical view]
PfamiPF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JKP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAERPEDLNL PNAVITRIIK EALPDGVNIS KEARSAISRA ASVFVLYATS
60 70 80 90 100
CANNFAMKGK RKTLNASDVL SAMEEMEFQR FITPLKEALE AYRRDEKGKK
110 120 130 140
EASEQKKKDK DKKDSEEQDK SREVEEEDEE RLDEDDQNEE EEIDN
Length:145
Mass (Da):16,629
Last modified:October 1, 2000 - v1
Checksum:i653B7D99E922E54F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti95 – 96DE → EQ in AAH24996 (PubMed:15489334).Curated2
Sequence conflicti124V → E in AAH24996 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF230806 mRNA. Translation: AAF67146.1.
BC024996 mRNA. Translation: AAH24996.1.
CCDSiCCDS18244.1.
RefSeqiNP_067473.2. NM_021498.2.
UniGeneiMm.27444.

Genome annotation databases

GeneIDi59001.
KEGGimmu:59001.
UCSCiuc008tfb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF230806 mRNA. Translation: AAF67146.1.
BC024996 mRNA. Translation: AAH24996.1.
CCDSiCCDS18244.1.
RefSeqiNP_067473.2. NM_021498.2.
UniGeneiMm.27444.

3D structure databases

ProteinModelPortaliQ9JKP7.
SMRiQ9JKP7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208472. 3 interactors.
IntActiQ9JKP7. 1 interactor.
STRINGi10090.ENSMUSP00000030091.

PTM databases

iPTMnetiQ9JKP7.
PhosphoSitePlusiQ9JKP7.

Proteomic databases

EPDiQ9JKP7.
MaxQBiQ9JKP7.
PaxDbiQ9JKP7.
PeptideAtlasiQ9JKP7.
PRIDEiQ9JKP7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi59001.
KEGGimmu:59001.
UCSCiuc008tfb.1. mouse.

Organism-specific databases

CTDi54107.
MGIiMGI:1933378. Pole3.

Phylogenomic databases

eggNOGiKOG0870. Eukaryota.
ENOG4111QSW. LUCA.
HOGENOMiHOG000007701.
HOVERGENiHBG060262.
InParanoidiQ9JKP7.
KOiK02326.
PhylomeDBiQ9JKP7.
TreeFamiTF103008.

Miscellaneous databases

PROiQ9JKP7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028394.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
[Graphical view]
PfamiPF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPOE3_MOUSE
AccessioniPrimary (citable) accession number: Q9JKP7
Secondary accession number(s): Q8R198
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.