Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9JKP5

- MBNL1_MOUSE

UniProt

Q9JKP5 - MBNL1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Muscleblind-like protein 1

Gene

Mbnl1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to CUG triplet repeat expansion in myotonic dystrophy muscle cells by sequestering the target RNAs (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri13 – 4129C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri47 – 7327C3H1-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri178 – 20629C3H1-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri214 – 24027C3H1-type 4PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. double-stranded RNA binding Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW
  3. RNA binding Source: UniProtKB

GO - Biological processi

  1. alternative mRNA splicing, via spliceosome Source: MGI
  2. embryonic limb morphogenesis Source: UniProtKB
  3. in utero embryonic development Source: UniProtKB
  4. mRNA splice site selection Source: MGI
  5. myoblast differentiation Source: UniProtKB
  6. nervous system development Source: UniProtKB
  7. regulation of alternative mRNA splicing, via spliceosome Source: MGI
  8. regulation of RNA splicing Source: UniProtKB
  9. RNA splicing Source: UniProtKB
  10. skeletal muscle tissue development Source: MGI
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Muscleblind-like protein 1
Alternative name(s):
Triplet-expansion RNA-binding protein
Gene namesi
Name:Mbnl1
Synonyms:Exp, Mbnl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1928482. Mbnl1.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity. Cytoplasmic granule By similarity
Note: Localized with DDX1, TIAL1 and YBX1 in stress granules upon stress. Localized in the cytoplasm of multinucleated myotubes (By similarity).By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytoplasmic stress granule Source: UniProtKB
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341Muscleblind-like protein 1PRO_0000089179Add
BLAST

Proteomic databases

MaxQBiQ9JKP5.
PaxDbiQ9JKP5.
PRIDEiQ9JKP5.

PTM databases

PhosphoSiteiQ9JKP5.

Expressioni

Tissue specificityi

Highly expressed in cardiac and skeletal muscle. Weakly expressed in heart and eye (at protein level).1 Publication

Gene expression databases

CleanExiMM_MBNL1.
GenevestigatoriQ9JKP5.

Interactioni

Subunit structurei

Interacts with DDX1 and YBX1. Interacts with HNRNPH1; the interaction in RNA-independent (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9JKP5. 1 interaction.
MINTiMINT-4101428.

Structurei

3D structure databases

ProteinModelPortaliQ9JKP5.
SMRiQ9JKP5. Positions 11-86, 177-243.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the muscleblind family.Curated
Contains 4 C3H1-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri13 – 4129C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri47 – 7327C3H1-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri178 – 20629C3H1-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri214 – 24027C3H1-type 4PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG241142.
HOGENOMiHOG000230928.
HOVERGENiHBG006999.
InParanoidiQ9JKP5.
KOiK14943.
PhylomeDBiQ9JKP5.

Family and domain databases

Gene3Di4.10.1000.10. 1 hit.
InterProiIPR000571. Znf_CCCH.
[Graphical view]
PfamiPF00642. zf-CCCH. 1 hit.
[Graphical view]
SMARTiSM00356. ZnF_C3H1. 4 hits.
[Graphical view]
PROSITEiPS50103. ZF_C3H1. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JKP5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVSVTPIRD TKWLTLEVCR EFQRGTCSRP DTECKFAHPS KSCQVENGRV
60 70 80 90 100
IACFDSLKGR CSRENCKYLH PPPHLKTQLE INGRNNLIQQ KNMAMLAQQM
110 120 130 140 150
QLANAMMPGA PLQPVPMFSV APSLATSASA AFNPYLGPVS PSLVPAEILP
160 170 180 190 200
TAPMLVTGNP GVPVPAAAAA AAQKLMRTDR LEVCREYQRG NCNRGENDCR
210 220 230 240 250
FAHPADSTMI DTNDNTVTVC MDYIKGRCSR EKCKYFHPPA HLQAKIKAAQ
260 270 280 290 300
YQVNQAAAAQ AAATAAAMGI PQAVLPPLPK RPALEKTNGA TAVFNTGIFQ
310 320 330 340
YQQALANMQL QQHTAFLPPG SILCMTPATS VDTHNICRTS D
Length:341
Mass (Da):36,976
Last modified:October 1, 2000 - v1
Checksum:i8E008DB5C7EF8AB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF231110 mRNA. Translation: AAF72159.1.
RefSeqiNP_001240637.1. NM_001253708.1.
UniGeneiMm.255723.

Genome annotation databases

GeneIDi56758.
KEGGimmu:56758.
UCSCiuc008pjf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF231110 mRNA. Translation: AAF72159.1 .
RefSeqi NP_001240637.1. NM_001253708.1.
UniGenei Mm.255723.

3D structure databases

ProteinModelPortali Q9JKP5.
SMRi Q9JKP5. Positions 11-86, 177-243.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9JKP5. 1 interaction.
MINTi MINT-4101428.

PTM databases

PhosphoSitei Q9JKP5.

Proteomic databases

MaxQBi Q9JKP5.
PaxDbi Q9JKP5.
PRIDEi Q9JKP5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 56758.
KEGGi mmu:56758.
UCSCi uc008pjf.1. mouse.

Organism-specific databases

CTDi 4154.
MGIi MGI:1928482. Mbnl1.

Phylogenomic databases

eggNOGi NOG241142.
HOGENOMi HOG000230928.
HOVERGENi HBG006999.
InParanoidi Q9JKP5.
KOi K14943.
PhylomeDBi Q9JKP5.

Miscellaneous databases

NextBioi 313286.
PROi Q9JKP5.
SOURCEi Search...

Gene expression databases

CleanExi MM_MBNL1.
Genevestigatori Q9JKP5.

Family and domain databases

Gene3Di 4.10.1000.10. 1 hit.
InterProi IPR000571. Znf_CCCH.
[Graphical view ]
Pfami PF00642. zf-CCCH. 1 hit.
[Graphical view ]
SMARTi SM00356. ZnF_C3H1. 4 hits.
[Graphical view ]
PROSITEi PS50103. ZF_C3H1. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning of mouse muscleblind."
    Castagnola P., Monticone M., Borsani G., Bassi M.T., Tonachini L.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-9, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  3. "Recruitment of human muscleblind proteins to (CUG)(n) expansions associated with myotonic dystrophy."
    Miller J.W., Urbinati C.R., Teng-Umnuay P., Stenberg M.G., Byrne B.J., Thornton C.A., Swanson M.S.
    EMBO J. 19:4439-4448(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiMBNL1_MOUSE
AccessioniPrimary (citable) accession number: Q9JKP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3