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Protein

Sphingosine 1-phosphate receptor 5

Gene

S1pr5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/O)alpha and G(12) subclass of heteromeric G-proteins. Displays antiproliferative effects in transfected CHO-K1 and HEK293 cells.

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: RGD
  2. sphingosine-1-phosphate receptor activity Source: InterPro

GO - Biological processi

  1. regulation of neuron differentiation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_233455. G alpha (i) signalling events.
REACT_239650. Lysosphingolipid and LPA receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine 1-phosphate receptor 5
Short name:
S1P receptor 5
Short name:
S1P5
Alternative name(s):
Endothelial differentiation G-protein-coupled receptor 8
Nerve growth factor-regulated G-protein-coupled receptor 1
Short name:
NRG-1
Sphingosine 1-phosphate receptor Edg-8
Short name:
S1P receptor Edg-8
Gene namesi
Name:S1pr5
Synonyms:Edg8, Nrg1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 8

Organism-specific databases

RGDi620566. S1pr5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4040ExtracellularBy similarityAdd
BLAST
Transmembranei41 – 6121Helical; Name=1By similarityAdd
BLAST
Topological domaini62 – 698CytoplasmicBy similarity
Transmembranei70 – 9021Helical; Name=2By similarityAdd
BLAST
Topological domaini91 – 11121ExtracellularBy similarityAdd
BLAST
Transmembranei112 – 13221Helical; Name=3By similarityAdd
BLAST
Topological domaini133 – 15119CytoplasmicBy similarityAdd
BLAST
Transmembranei152 – 17221Helical; Name=4By similarityAdd
BLAST
Topological domaini173 – 19220ExtracellularBy similarityAdd
BLAST
Transmembranei193 – 21321Helical; Name=5By similarityAdd
BLAST
Topological domaini214 – 25340CytoplasmicBy similarityAdd
BLAST
Transmembranei254 – 27421Helical; Name=6By similarityAdd
BLAST
Topological domaini275 – 28814ExtracellularBy similarityAdd
BLAST
Transmembranei289 – 30921Helical; Name=7By similarityAdd
BLAST
Topological domaini310 – 40091CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: RGD
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Sphingosine 1-phosphate receptor 5PRO_0000069439Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi20 – 201N-linked (GlcNAc...)Sequence Analysis
Lipidationi324 – 3241S-palmitoyl cysteineBy similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ9JKM5.
PRIDEiQ9JKM5.

PTM databases

PhosphoSiteiQ9JKM5.

Expressioni

Tissue specificityi

Found almost exclusively in brain and skin. Ubiquitously detected in different brain regions with very prominent expression in lower brain regions such as the midbrain, pons, medulla, and spinal cord. Expressed predominantly within white matter tracts of the brain. Corpus callosum, optic nerve, olfactory tract, anterior commissure, internal and external capsules, fimbra of the hippocampus, mammillary tract, stria medullaris, and white matter of the cerebellum and brain stem all express the receptor. Within the striatum itself white matter fascicles express the receptor. Detected also in spleen and lung. Not observed in heart, liver, skeletal muscle, kidney, or testes.3 Publications

Inductioni

In PC-12 cells, nerve growth factor induces neuronal differentiation and represses expression of the receptor. Down-regulated also by fibroblast growth factor and dibutyryl cAMP. Epidermal growth factor, an agent that does not induce differentiation, does not repress expression. Protein kinase A appears to be an obligatory cellular component in regulation.1 Publication

Gene expression databases

GenevestigatoriQ9JKM5.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028380.

Structurei

3D structure databases

ProteinModelPortaliQ9JKM5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG135858.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000233501.
HOVERGENiHBG103071.
InParanoidiQ9JKM5.
KOiK04295.
OMAiPIIYTFT.
OrthoDBiEOG708W0B.
PhylomeDBiQ9JKM5.
TreeFamiTF330052.

Family and domain databases

InterProiIPR005386. EDG8_S1P_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR004061. S1P_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01561. EDG8RECEPTOR.
PR00237. GPCRRHODOPSN.
PR01523. S1PRECEPTOR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JKM5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESGLLRPAP VSEVIVLHYN YTGKLRGARY QPGAGLRADA AVCLAVCAFI
60 70 80 90 100
VLENLAVLLV LGRHPRFHAP MFLLLGSLTL SDLLAGAAYA TNILLSGPLT
110 120 130 140 150
LRLSPALWFA REGGVFVALA ASVLSLLAIA LERHLTMARR GPAPAASRAR
160 170 180 190 200
TLAMAVAAWG LSLLLGLLPA LGWNCLGRLE ACSTVLPLYA KAYVLFCVLA
210 220 230 240 250
FLGILAAICA LYARIYCQVR ANARRLRAGP GSRRATSSSR SRHTPRSLAL
260 270 280 290 300
LRTLSVVLLA FVACWGPLFL LLLLDVACPA RACPVLLQAD PFLGLAMANS
310 320 330 340 350
LLNPIIYTFT NRDLRHALLR LLCCGRGPCN QDSSNSLQRS PSAVGPSGGG
360 370 380 390 400
LRRCLPPTLD RSSSPSEHSC PQRDGMDTSC STGSPGAATA NRTLVPDATD
Length:400
Mass (Da):42,369
Last modified:October 1, 2000 - v1
Checksum:i71B398DC4DEE0FEB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti131 – 1311L → I in AAF15395 (PubMed:10532805).Curated
Sequence conflicti162 – 1643SLL → LLT in AAF15395 (PubMed:10532805).Curated
Sequence conflicti188 – 1881L → V in AAF15395 (PubMed:10532805).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF115249 mRNA. Translation: AAF15395.1.
AF233649 mRNA. Translation: AAF35912.1.
RefSeqiNP_068543.2. NM_021775.2.
XP_006242725.1. XM_006242663.2.
XP_006242726.1. XM_006242664.2.
XP_006242727.1. XM_006242665.2.
UniGeneiRn.45601.

Genome annotation databases

EnsembliENSRNOT00000028380; ENSRNOP00000028380; ENSRNOG00000020901.
GeneIDi60399.
KEGGirno:60399.
UCSCiRGD:620566. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF115249 mRNA. Translation: AAF15395.1.
AF233649 mRNA. Translation: AAF35912.1.
RefSeqiNP_068543.2. NM_021775.2.
XP_006242725.1. XM_006242663.2.
XP_006242726.1. XM_006242664.2.
XP_006242727.1. XM_006242665.2.
UniGeneiRn.45601.

3D structure databases

ProteinModelPortaliQ9JKM5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028380.

Chemistry

GuidetoPHARMACOLOGYi279.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ9JKM5.

Proteomic databases

PaxDbiQ9JKM5.
PRIDEiQ9JKM5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028380; ENSRNOP00000028380; ENSRNOG00000020901.
GeneIDi60399.
KEGGirno:60399.
UCSCiRGD:620566. rat.

Organism-specific databases

CTDi53637.
RGDi620566. S1pr5.

Phylogenomic databases

eggNOGiNOG135858.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000233501.
HOVERGENiHBG103071.
InParanoidiQ9JKM5.
KOiK04295.
OMAiPIIYTFT.
OrthoDBiEOG708W0B.
PhylomeDBiQ9JKM5.
TreeFamiTF330052.

Enzyme and pathway databases

ReactomeiREACT_233455. G alpha (i) signalling events.
REACT_239650. Lysosphingolipid and LPA receptors.

Miscellaneous databases

NextBioi612112.
PROiQ9JKM5.

Gene expression databases

GenevestigatoriQ9JKM5.

Family and domain databases

InterProiIPR005386. EDG8_S1P_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR004061. S1P_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01561. EDG8RECEPTOR.
PR00237. GPCRRHODOPSN.
PR01523. S1PRECEPTOR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning, tissue-specific expression, and chromosomal localization of a novel nerve growth factor-regulated G-protein-coupled receptor, nrg-1."
    Glickman M., Malek R.L., Kwitek-Black A.E., Jacob H.J., Lee N.H.
    Mol. Cell. Neurosci. 14:141-152(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
    Tissue: Adrenal gland.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, POSSIBLE FUNCTION.
    Tissue: Brain.
  3. "Nrg-1 belongs to the endothelial differentiation gene family of G protein-coupled sphingosine-1-phosphate receptors."
    Malek R.L., Toman R.E., Edsall L.C., Wong S., Chiu J., Letterle C.A., Van Brocklyn J.R., Milstien S., Spiegel S., Lee N.H.
    J. Biol. Chem. 276:5692-5699(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SIGNAL TRANSDUCTION PATHWAY EXAMINATION.
  4. "Comparative analysis of human and rat S1P(5) (edg8): differential expression profiles and sensitivities to antagonists."
    Niedernberg A., Scherer C.R., Busch A.E., Kostenis E.
    Biochem. Pharmacol. 64:1243-1250(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiS1PR5_RAT
AccessioniPrimary (citable) accession number: Q9JKM5
Secondary accession number(s): Q9QY79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: October 1, 2000
Last modified: January 7, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.