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Q9JKM5 (S1PR5_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sphingosine 1-phosphate receptor 5

Short name=S1P receptor 5
Short name=S1P5
Alternative name(s):
Endothelial differentiation G-protein-coupled receptor 8
Nerve growth factor-regulated G-protein-coupled receptor 1
Short name=NRG-1
Sphingosine 1-phosphate receptor Edg-8
Short name=S1P receptor Edg-8
Gene names
Name:S1pr5
Synonyms:Edg8, Nrg1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length400 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/O)alpha and G12 subclass of heteromeric G-proteins. Displays antiproliferative effects in transfected CHO-K1 and HEK293 cells. Ref.2

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Found almost exclusively in brain and skin. Ubiquitously detected in different brain regions with very prominent expression in lower brain regions such as the midbrain, pons, medulla, and spinal cord. Expressed predominantly within white matter tracts of the brain. Corpus callosum, optic nerve, olfactory tract, anterior commissure, internal and external capsules, fimbra of the hippocampus, mammillary tract, stria medullaris, and white matter of the cerebellum and brain stem all express the receptor. Within the striatum itself white matter fascicles express the receptor. Detected also in spleen and lung. Not observed in heart, liver, skeletal muscle, kidney, or testes. Ref.1 Ref.2 Ref.4

Induction

In PC-12 cells, nerve growth factor induces neuronal differentiation and represses expression of the receptor. Down-regulated also by fibroblast growth factor and dibutyryl cAMP. Epidermal growth factor, an agent that does not induce differentiation, does not repress expression. Protein kinase A appears to be an obligatory cellular component in regulation. Ref.1

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 400400Sphingosine 1-phosphate receptor 5
PRO_0000069439

Regions

Topological domain1 – 4040Extracellular By similarity
Transmembrane41 – 6121Helical; Name=1; By similarity
Topological domain62 – 698Cytoplasmic By similarity
Transmembrane70 – 9021Helical; Name=2; By similarity
Topological domain91 – 11121Extracellular By similarity
Transmembrane112 – 13221Helical; Name=3; By similarity
Topological domain133 – 15119Cytoplasmic By similarity
Transmembrane152 – 17221Helical; Name=4; By similarity
Topological domain173 – 19220Extracellular By similarity
Transmembrane193 – 21321Helical; Name=5; By similarity
Topological domain214 – 25340Cytoplasmic By similarity
Transmembrane254 – 27421Helical; Name=6; By similarity
Topological domain275 – 28814Extracellular By similarity
Transmembrane289 – 30921Helical; Name=7; By similarity
Topological domain310 – 40091Cytoplasmic By similarity

Amino acid modifications

Lipidation3241S-palmitoyl cysteine By similarity
Glycosylation201N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict1311L → I in AAF15395. Ref.1
Sequence conflict162 – 1643SLL → LLT in AAF15395. Ref.1
Sequence conflict1881L → V in AAF15395. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9JKM5 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 71B398DC4DEE0FEB

FASTA40042,369
        10         20         30         40         50         60 
MESGLLRPAP VSEVIVLHYN YTGKLRGARY QPGAGLRADA AVCLAVCAFI VLENLAVLLV 

        70         80         90        100        110        120 
LGRHPRFHAP MFLLLGSLTL SDLLAGAAYA TNILLSGPLT LRLSPALWFA REGGVFVALA 

       130        140        150        160        170        180 
ASVLSLLAIA LERHLTMARR GPAPAASRAR TLAMAVAAWG LSLLLGLLPA LGWNCLGRLE 

       190        200        210        220        230        240 
ACSTVLPLYA KAYVLFCVLA FLGILAAICA LYARIYCQVR ANARRLRAGP GSRRATSSSR 

       250        260        270        280        290        300 
SRHTPRSLAL LRTLSVVLLA FVACWGPLFL LLLLDVACPA RACPVLLQAD PFLGLAMANS 

       310        320        330        340        350        360 
LLNPIIYTFT NRDLRHALLR LLCCGRGPCN QDSSNSLQRS PSAVGPSGGG LRRCLPPTLD 

       370        380        390        400 
RSSSPSEHSC PQRDGMDTSC STGSPGAATA NRTLVPDATD 

« Hide

References

[1]"Molecular cloning, tissue-specific expression, and chromosomal localization of a novel nerve growth factor-regulated G-protein-coupled receptor, nrg-1."
Glickman M., Malek R.L., Kwitek-Black A.E., Jacob H.J., Lee N.H.
Mol. Cell. Neurosci. 14:141-152(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
Tissue: Adrenal gland.
[2]"Characterization of a novel sphingosine 1-phosphate receptor, Edg-8."
Im D.-S., Heise C.E., Ancellin N., O'Dowd B.F., Shei G.-J., Heavens R.P., Rigby M.R., Hla T., Mandala S., McAllister G., George S.R., Lynch K.R.
J. Biol. Chem. 275:14281-14286(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, POSSIBLE FUNCTION.
Tissue: Brain.
[3]"Nrg-1 belongs to the endothelial differentiation gene family of G protein-coupled sphingosine-1-phosphate receptors."
Malek R.L., Toman R.E., Edsall L.C., Wong S., Chiu J., Letterle C.A., Van Brocklyn J.R., Milstien S., Spiegel S., Lee N.H.
J. Biol. Chem. 276:5692-5699(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SIGNAL TRANSDUCTION PATHWAY EXAMINATION.
[4]"Comparative analysis of human and rat S1P(5) (edg8): differential expression profiles and sensitivities to antagonists."
Niedernberg A., Scherer C.R., Busch A.E., Kostenis E.
Biochem. Pharmacol. 64:1243-1250(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF115249 mRNA. Translation: AAF15395.1.
AF233649 mRNA. Translation: AAF35912.1.
RefSeqNP_068543.2. NM_021775.2.
XP_006242725.1. XM_006242663.1.
XP_006242726.1. XM_006242664.1.
XP_006242727.1. XM_006242665.1.
UniGeneRn.45601.

3D structure databases

ProteinModelPortalQ9JKM5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000028380.

Chemistry

GuidetoPHARMACOLOGY279.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ9JKM5.

Proteomic databases

PaxDbQ9JKM5.
PRIDEQ9JKM5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000028380; ENSRNOP00000028380; ENSRNOG00000020901.
GeneID60399.
KEGGrno:60399.
UCSCRGD:620566. rat.

Organism-specific databases

CTD53637.
RGD620566. S1pr5.

Phylogenomic databases

eggNOGNOG135858.
GeneTreeENSGT00750000117544.
HOGENOMHOG000233501.
HOVERGENHBG103071.
InParanoidQ9JKM5.
KOK04295.
OMAPIIYTFT.
OrthoDBEOG708W0B.
PhylomeDBQ9JKM5.
TreeFamTF330052.

Gene expression databases

GenevestigatorQ9JKM5.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR005386. EDG8_S1P_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR004061. S1P_rcpt.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR01561. EDG8RECEPTOR.
PR00237. GPCRRHODOPSN.
PR01523. S1PRECEPTOR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio612112.
PROQ9JKM5.

Entry information

Entry nameS1PR5_RAT
AccessionPrimary (citable) accession number: Q9JKM5
Secondary accession number(s): Q9QY79
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries