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Q9JKM5

- S1PR5_RAT

UniProt

Q9JKM5 - S1PR5_RAT

Protein

Sphingosine 1-phosphate receptor 5

Gene

S1pr5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/O)alpha and G(12) subclass of heteromeric G-proteins. Displays antiproliferative effects in transfected CHO-K1 and HEK293 cells.

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: RGD
    2. sphingosine-1-phosphate receptor activity Source: InterPro

    GO - Biological processi

    1. regulation of neuron differentiation Source: RGD

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sphingosine 1-phosphate receptor 5
    Short name:
    S1P receptor 5
    Short name:
    S1P5
    Alternative name(s):
    Endothelial differentiation G-protein-coupled receptor 8
    Nerve growth factor-regulated G-protein-coupled receptor 1
    Short name:
    NRG-1
    Sphingosine 1-phosphate receptor Edg-8
    Short name:
    S1P receptor Edg-8
    Gene namesi
    Name:S1pr5
    Synonyms:Edg8, Nrg1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 8

    Organism-specific databases

    RGDi620566. S1pr5.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: RGD
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 400400Sphingosine 1-phosphate receptor 5PRO_0000069439Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi20 – 201N-linked (GlcNAc...)Sequence Analysis
    Lipidationi324 – 3241S-palmitoyl cysteineBy similarity

    Keywords - PTMi

    Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiQ9JKM5.
    PRIDEiQ9JKM5.

    PTM databases

    PhosphoSiteiQ9JKM5.

    Expressioni

    Tissue specificityi

    Found almost exclusively in brain and skin. Ubiquitously detected in different brain regions with very prominent expression in lower brain regions such as the midbrain, pons, medulla, and spinal cord. Expressed predominantly within white matter tracts of the brain. Corpus callosum, optic nerve, olfactory tract, anterior commissure, internal and external capsules, fimbra of the hippocampus, mammillary tract, stria medullaris, and white matter of the cerebellum and brain stem all express the receptor. Within the striatum itself white matter fascicles express the receptor. Detected also in spleen and lung. Not observed in heart, liver, skeletal muscle, kidney, or testes.3 Publications

    Inductioni

    In PC-12 cells, nerve growth factor induces neuronal differentiation and represses expression of the receptor. Down-regulated also by fibroblast growth factor and dibutyryl cAMP. Epidermal growth factor, an agent that does not induce differentiation, does not repress expression. Protein kinase A appears to be an obligatory cellular component in regulation.1 Publication

    Gene expression databases

    GenevestigatoriQ9JKM5.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000028380.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JKM5.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4040ExtracellularBy similarityAdd
    BLAST
    Topological domaini62 – 698CytoplasmicBy similarity
    Topological domaini91 – 11121ExtracellularBy similarityAdd
    BLAST
    Topological domaini133 – 15119CytoplasmicBy similarityAdd
    BLAST
    Topological domaini173 – 19220ExtracellularBy similarityAdd
    BLAST
    Topological domaini214 – 25340CytoplasmicBy similarityAdd
    BLAST
    Topological domaini275 – 28814ExtracellularBy similarityAdd
    BLAST
    Topological domaini310 – 40091CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei41 – 6121Helical; Name=1By similarityAdd
    BLAST
    Transmembranei70 – 9021Helical; Name=2By similarityAdd
    BLAST
    Transmembranei112 – 13221Helical; Name=3By similarityAdd
    BLAST
    Transmembranei152 – 17221Helical; Name=4By similarityAdd
    BLAST
    Transmembranei193 – 21321Helical; Name=5By similarityAdd
    BLAST
    Transmembranei254 – 27421Helical; Name=6By similarityAdd
    BLAST
    Transmembranei289 – 30921Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG135858.
    GeneTreeiENSGT00750000117544.
    HOGENOMiHOG000233501.
    HOVERGENiHBG103071.
    InParanoidiQ9JKM5.
    KOiK04295.
    OMAiPIIYTFT.
    OrthoDBiEOG708W0B.
    PhylomeDBiQ9JKM5.
    TreeFamiTF330052.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR005386. EDG8_S1P_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR004061. S1P_rcpt.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR01561. EDG8RECEPTOR.
    PR00237. GPCRRHODOPSN.
    PR01523. S1PRECEPTOR.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9JKM5-1 [UniParc]FASTAAdd to Basket

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    MESGLLRPAP VSEVIVLHYN YTGKLRGARY QPGAGLRADA AVCLAVCAFI    50
    VLENLAVLLV LGRHPRFHAP MFLLLGSLTL SDLLAGAAYA TNILLSGPLT 100
    LRLSPALWFA REGGVFVALA ASVLSLLAIA LERHLTMARR GPAPAASRAR 150
    TLAMAVAAWG LSLLLGLLPA LGWNCLGRLE ACSTVLPLYA KAYVLFCVLA 200
    FLGILAAICA LYARIYCQVR ANARRLRAGP GSRRATSSSR SRHTPRSLAL 250
    LRTLSVVLLA FVACWGPLFL LLLLDVACPA RACPVLLQAD PFLGLAMANS 300
    LLNPIIYTFT NRDLRHALLR LLCCGRGPCN QDSSNSLQRS PSAVGPSGGG 350
    LRRCLPPTLD RSSSPSEHSC PQRDGMDTSC STGSPGAATA NRTLVPDATD 400
    Length:400
    Mass (Da):42,369
    Last modified:October 1, 2000 - v1
    Checksum:i71B398DC4DEE0FEB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti131 – 1311L → I in AAF15395. (PubMed:10532805)Curated
    Sequence conflicti162 – 1643SLL → LLT in AAF15395. (PubMed:10532805)Curated
    Sequence conflicti188 – 1881L → V in AAF15395. (PubMed:10532805)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF115249 mRNA. Translation: AAF15395.1.
    AF233649 mRNA. Translation: AAF35912.1.
    RefSeqiNP_068543.2. NM_021775.2.
    XP_006242725.1. XM_006242663.1.
    XP_006242726.1. XM_006242664.1.
    XP_006242727.1. XM_006242665.1.
    UniGeneiRn.45601.

    Genome annotation databases

    EnsembliENSRNOT00000028380; ENSRNOP00000028380; ENSRNOG00000020901.
    GeneIDi60399.
    KEGGirno:60399.
    UCSCiRGD:620566. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF115249 mRNA. Translation: AAF15395.1 .
    AF233649 mRNA. Translation: AAF35912.1 .
    RefSeqi NP_068543.2. NM_021775.2.
    XP_006242725.1. XM_006242663.1.
    XP_006242726.1. XM_006242664.1.
    XP_006242727.1. XM_006242665.1.
    UniGenei Rn.45601.

    3D structure databases

    ProteinModelPortali Q9JKM5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000028380.

    Chemistry

    GuidetoPHARMACOLOGYi 279.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei Q9JKM5.

    Proteomic databases

    PaxDbi Q9JKM5.
    PRIDEi Q9JKM5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000028380 ; ENSRNOP00000028380 ; ENSRNOG00000020901 .
    GeneIDi 60399.
    KEGGi rno:60399.
    UCSCi RGD:620566. rat.

    Organism-specific databases

    CTDi 53637.
    RGDi 620566. S1pr5.

    Phylogenomic databases

    eggNOGi NOG135858.
    GeneTreei ENSGT00750000117544.
    HOGENOMi HOG000233501.
    HOVERGENi HBG103071.
    InParanoidi Q9JKM5.
    KOi K04295.
    OMAi PIIYTFT.
    OrthoDBi EOG708W0B.
    PhylomeDBi Q9JKM5.
    TreeFami TF330052.

    Miscellaneous databases

    NextBioi 612112.
    PROi Q9JKM5.

    Gene expression databases

    Genevestigatori Q9JKM5.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR005386. EDG8_S1P_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR004061. S1P_rcpt.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR01561. EDG8RECEPTOR.
    PR00237. GPCRRHODOPSN.
    PR01523. S1PRECEPTOR.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, tissue-specific expression, and chromosomal localization of a novel nerve growth factor-regulated G-protein-coupled receptor, nrg-1."
      Glickman M., Malek R.L., Kwitek-Black A.E., Jacob H.J., Lee N.H.
      Mol. Cell. Neurosci. 14:141-152(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
      Tissue: Adrenal gland.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, POSSIBLE FUNCTION.
      Tissue: Brain.
    3. "Nrg-1 belongs to the endothelial differentiation gene family of G protein-coupled sphingosine-1-phosphate receptors."
      Malek R.L., Toman R.E., Edsall L.C., Wong S., Chiu J., Letterle C.A., Van Brocklyn J.R., Milstien S., Spiegel S., Lee N.H.
      J. Biol. Chem. 276:5692-5699(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SIGNAL TRANSDUCTION PATHWAY EXAMINATION.
    4. "Comparative analysis of human and rat S1P(5) (edg8): differential expression profiles and sensitivities to antagonists."
      Niedernberg A., Scherer C.R., Busch A.E., Kostenis E.
      Biochem. Pharmacol. 64:1243-1250(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiS1PR5_RAT
    AccessioniPrimary (citable) accession number: Q9JKM5
    Secondary accession number(s): Q9QY79
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 12, 2005
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3