Sphingosine 1-phosphate receptor 5 - Rattus norvegicus (Rat)

Contribute

Send feedback

Read comments (?) or add your own

Q9JKM5 (S1PR5_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 86. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Sphingosine 1-phosphate receptor 5
Short name=S1P receptor 5
Short name=S1P5

Alternative name(s):
Endothelial differentiation G-protein-coupled receptor 8
Nerve growth factor-regulated G-protein-coupled receptor 1
Short name=NRG-1
Sphingosine 1-phosphate receptor Edg-8
Short name=S1P receptor Edg-8

Gene names

Name:	S1pr5
Synonyms:	Edg8, Nrg1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/O)alpha and G₁₂ subclass of heteromeric G-proteins. Displays antiproliferative effects in transfected CHO-K1 and HEK293 cells. Ref.2
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Found almost exclusively in brain and skin. Ubiquitously detected in different brain regions with very prominent expression in lower brain regions such as the midbrain, pons, medulla, and spinal cord. Expressed predominantly within white matter tracts of the brain. Corpus callosum, optic nerve, olfactory tract, anterior commissure, internal and external capsules, fimbra of the hippocampus, mammillary tract, stria medullaris, and white matter of the cerebellum and brain stem all express the receptor. Within the striatum itself white matter fascicles express the receptor. Detected also in spleen and lung. Not observed in heart, liver, skeletal muscle, kidney, or testes. Ref.1 Ref.2 Ref.4
Induction	In PC-12 cells, nerve growth factor induces neuronal differentiation and represses expression of the receptor. Down-regulated also by fibroblast growth factor and dibutyryl cAMP. Epidermal growth factor, an agent that does not induce differentiation, does not repress expression. Protein kinase A appears to be an obligatory cellular component in regulation. Ref.1
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of neuron differentiation Inferred from expression pattern PubMed 12617946. Source: RGD sphingosine-1-phosphate signaling pathway Inferred from electronic annotation. Source: GOC
Cellular_component	integral to membrane Inferred from direct assay Ref.2. Source: RGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor activity Inferred from direct assay Ref.2. Source: RGD sphingosine-1-phosphate receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 400

400

Sphingosine 1-phosphate receptor 5

PRO_0000069439

Regions

Topological domain

1 – 40

Extracellular By similarity

Transmembrane

41 – 61

Helical; Name=1; By similarity

Topological domain

62 – 69

Cytoplasmic By similarity

Transmembrane

70 – 90

Helical; Name=2; By similarity

Topological domain

91 – 111

Extracellular By similarity

Transmembrane

112 – 132

Helical; Name=3; By similarity

Topological domain

133 – 151

Cytoplasmic By similarity

Transmembrane

152 – 172

Helical; Name=4; By similarity

Topological domain

173 – 192

Extracellular By similarity

Transmembrane

193 – 213

Helical; Name=5; By similarity

Topological domain

214 – 253

Cytoplasmic By similarity

Transmembrane

254 – 274

Helical; Name=6; By similarity

Topological domain

275 – 288

Extracellular By similarity

Transmembrane

289 – 309

Helical; Name=7; By similarity

Topological domain

310 – 400

Cytoplasmic By similarity

Amino acid modifications

Lipidation

324

S-palmitoyl cysteine By similarity

Glycosylation

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

131

L → I in AAF15395. Ref.1

Sequence conflict

162 – 164

SLL → LLT in AAF15395. Ref.1

Sequence conflict

188

L → V in AAF15395. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q9JKM5 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 71B398DC4DEE0FEB
Show »

FASTA

400

42,369

        10         20         30         40         50         60 
MESGLLRPAP VSEVIVLHYN YTGKLRGARY QPGAGLRADA AVCLAVCAFI VLENLAVLLV 

        70         80         90        100        110        120 
LGRHPRFHAP MFLLLGSLTL SDLLAGAAYA TNILLSGPLT LRLSPALWFA REGGVFVALA 

       130        140        150        160        170        180 
ASVLSLLAIA LERHLTMARR GPAPAASRAR TLAMAVAAWG LSLLLGLLPA LGWNCLGRLE 

       190        200        210        220        230        240 
ACSTVLPLYA KAYVLFCVLA FLGILAAICA LYARIYCQVR ANARRLRAGP GSRRATSSSR 

       250        260        270        280        290        300 
SRHTPRSLAL LRTLSVVLLA FVACWGPLFL LLLLDVACPA RACPVLLQAD PFLGLAMANS 

       310        320        330        340        350        360 
LLNPIIYTFT NRDLRHALLR LLCCGRGPCN QDSSNSLQRS PSAVGPSGGG LRRCLPPTLD 

       370        380        390        400 
RSSSPSEHSC PQRDGMDTSC STGSPGAATA NRTLVPDATD

« Hide

References

[1]	"Molecular cloning, tissue-specific expression, and chromosomal localization of a novel nerve growth factor-regulated G-protein-coupled receptor, nrg-1." Glickman M., Malek R.L., Kwitek-Black A.E., Jacob H.J., Lee N.H. Mol. Cell. Neurosci. 14:141-152(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION. Tissue: Adrenal gland.
[2]	"Characterization of a novel sphingosine 1-phosphate receptor, Edg-8." Im D.-S., Heise C.E., Ancellin N., O'Dowd B.F., Shei G.-J., Heavens R.P., Rigby M.R., Hla T., Mandala S., McAllister G., George S.R., Lynch K.R. J. Biol. Chem. 275:14281-14286(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, POSSIBLE FUNCTION. Tissue: Brain.
[3]	"Nrg-1 belongs to the endothelial differentiation gene family of G protein-coupled sphingosine-1-phosphate receptors." Malek R.L., Toman R.E., Edsall L.C., Wong S., Chiu J., Letterle C.A., Van Brocklyn J.R., Milstien S., Spiegel S., Lee N.H. J. Biol. Chem. 276:5692-5699(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SIGNAL TRANSDUCTION PATHWAY EXAMINATION.
[4]	"Comparative analysis of human and rat S1P(5) (edg8): differential expression profiles and sensitivities to antagonists." Niedernberg A., Scherer C.R., Busch A.E., Kostenis E. Biochem. Pharmacol. 64:1243-1250(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF115249 mRNA. Translation: AAF15395.1. AF233649 mRNA. Translation: AAF35912.1.
IPI	IPI00202800.
RefSeq	NP_068543.2. NM_021775.2.
UniGene	Rn.45601.
3D structure databases
ProteinModelPortal	Q9JKM5.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000028380.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9JKM5.
Proteomic databases
PaxDb	Q9JKM5.
PRIDE	Q9JKM5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000028380; ENSRNOP00000028380; ENSRNOG00000020901.
GeneID	60399.
KEGG	rno:60399.
UCSC	RGD:620566. rat.
Organism-specific databases
CTD	53637.
RGD	620566. S1pr5.
Phylogenomic databases
eggNOG	NOG135858.
GeneTree	ENSGT00700000104088.
HOGENOM	HOG000233501.
HOVERGEN	HBG103071.
InParanoid	Q9JKM5.
KO	K04295.
OMA	PIIYTFT.
OrthoDB	EOG4PNXHD.
Gene expression databases
Genevestigator	Q9JKM5.
GermOnline	ENSRNOG00000020901. Rattus norvegicus.
Family and domain databases
InterPro	IPR005386. EDG8_S1P_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR004061. S1P_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01561. EDG8RECEPTOR. PR00237. GPCRRHODOPSN. PR01523. S1PRECEPTOR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	612112.

Entry information

Entry name

S1PR5_RAT

Accession

Primary (citable) accession number: Q9JKM5
Secondary accession number(s): Q9QY79

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents