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Protein

Splicing regulatory glutamine/lysine-rich protein 1

Gene

Srek1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the regulation of alternative splicing by modulating the activity of other splice facors. Inhibits the splicing activity of SFRS1, SFRS2 and SFRS6. Augments the splicing activity of SFRS3.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing regulatory glutamine/lysine-rich protein 1
Alternative name(s):
SR-related protein of 86 kDa
Serine/arginine-rich-splicing regulatory protein 86
Short name:
SRrp86
Splicing factor, arginine/serine-rich 12
Gene namesi
Name:Srek1
Synonyms:Sfrs12, Srrp86
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621696. Srek1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Splicing regulatory glutamine/lysine-rich protein 1PRO_0000081942Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei187 – 1871PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9JKL7.
PRIDEiQ9JKL7.

Expressioni

Tissue specificityi

Ubiquitous. Detected in liver, brain, lung, spleen, testis and pancreas.1 Publication

Interactioni

Subunit structurei

Interacts with SREK1IP1 (By similarity). Homodimer. Binds SFRS1, SFRS2, SFRS3 and SFRS6. Interacts with the spliceosome.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DDX17Q928413EBI-6452221,EBI-746012From a different organism.
RBMXP381593EBI-6452221,EBI-743526From a different organism.
SAFBQ154243EBI-6452221,EBI-348298From a different organism.
SRSF3P841033EBI-6452221,EBI-372557From a different organism.
SRSF7Q166293EBI-6452221,EBI-398885From a different organism.

Protein-protein interaction databases

IntActiQ9JKL7. 53 interactions.
STRINGi10116.ENSRNOP00000049050.

Structurei

3D structure databases

ProteinModelPortaliQ9JKL7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini69 – 14577RRMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi190 – 473284Arg/Glu/Lys/Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4676. Eukaryota.
ENOG410YYIR. LUCA.
HOVERGENiHBG079174.
InParanoidiQ9JKL7.
KOiK13165.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JKL7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSLVPGAGL LPIPTSSPLT AVSSLGVSLS SLGAIPAAAL DPNITALGEI
60 70 80 90 100
PQPPLMGNVD PSKIDEIRRT VYVGNLNSQT TTADQLLEFF KQVGEVKFVR
110 120 130 140 150
MAGDETQPTR FAFVEFADQN SVPRALAFNG VMFGDRPLKI NHSNNAIVKP
160 170 180 190 200
PEMTPQAAAK ELEEVMKRVR EAQSFISAAI EPESGKSNER KGGRSRSHTR
210 220 230 240 250
SKSRSSSKSH SRRKRSQSKH RSRSHNRSRS RQKDRARSKS PHKKRSKSRE
260 270 280 290 300
RRKSRSRSRS RDKRKDTREK VKERVKERER EKEKEREKER EKDKERGKNK
310 320 330 340 350
DKDREKEKDH EKERDKEKEK EQDKDKEREK DRSKEADEKR KKEKKSRTPP
360 370 380 390 400
RSYNSSRRSR SASRERRRRR SRSSSRSPRT SKTIKRKSSR SPSPRGRNKK
410 420 430 440 450
EKKREKERDH ISDRRERERS TSTKKSSSDR DGKEKVEKTN TPVKEKEHSK
460 470 480 490
EADSTVSTDA DEKDTARTEE ESKAQHNGNC QPHEERLCST ADAV
Length:494
Mass (Da):56,849
Last modified:October 1, 2000 - v1
Checksum:i1BD81112FAD6132E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF234765 mRNA. Translation: AAF37578.1.
RefSeqiNP_064477.1. NM_020092.1.
UniGeneiRn.161755.
Rn.53896.

Genome annotation databases

GeneIDi56763.
KEGGirno:56763.
UCSCiRGD:621696. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF234765 mRNA. Translation: AAF37578.1.
RefSeqiNP_064477.1. NM_020092.1.
UniGeneiRn.161755.
Rn.53896.

3D structure databases

ProteinModelPortaliQ9JKL7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JKL7. 53 interactions.
STRINGi10116.ENSRNOP00000049050.

Proteomic databases

PaxDbiQ9JKL7.
PRIDEiQ9JKL7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi56763.
KEGGirno:56763.
UCSCiRGD:621696. rat.

Organism-specific databases

CTDi140890.
RGDi621696. Srek1.

Phylogenomic databases

eggNOGiKOG4676. Eukaryota.
ENOG410YYIR. LUCA.
HOVERGENiHBG079174.
InParanoidiQ9JKL7.
KOiK13165.

Miscellaneous databases

PROiQ9JKL7.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification and characterization of a novel serine-arginine-rich splicing regulatory protein."
    Barnard D.C., Patton J.G.
    Mol. Cell. Biol. 20:3049-3057(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, HOMODIMERIZATION, INTERACTION WITH SFRS1; SFRS2; SFRS3 AND SFRS6.
    Tissue: Brain.
  2. "Regulation of alternative splicing by SRrp86 through coactivation and repression of specific SR proteins."
    Barnard D.C., Li J., Peng R., Patton J.G.
    RNA 8:526-533(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH SFRS2 AND SFRS3.

Entry informationi

Entry nameiSREK1_RAT
AccessioniPrimary (citable) accession number: Q9JKL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.