Q9JKL1 (PKR1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prokineticin receptor 1
Short name=PK-R1

Alternative name(s):
G-protein coupled receptor 73

Gene names

Name:	Prokr1
Synonyms:	Gpr73, Pkr1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for prokineticin 1. Exclusively coupled to the G(q) subclass of heteromeric G proteins. Activation leads to mobilization of calcium, stimulation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed at high levels in the heart, skeletal muscle and pancreas. Expressed at lower levels in the brain, lung, liver and kidney.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	circadian rhythm Traceable author statement Ref.2. Source: MGI coronary vasculature development Traceable author statement PubMed 20299672. Source: DFLAT negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor activity Traceable author statement Ref.2. Source: MGI neuropeptide Y receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Prokineticin receptor 1		PRO_0000070080
Regions
	Topological domain	1 – 62	62	Extracellular Potential
	Transmembrane	63 – 83	21	Helical; Name=1; Potential
	Topological domain	84 – 98	15	Cytoplasmic Potential
	Transmembrane	99 – 119	21	Helical; Name=2; Potential
	Topological domain	120 – 146	27	Extracellular Potential
	Transmembrane	147 – 167	21	Helical; Name=3; Potential
	Topological domain	168 – 179	12	Cytoplasmic Potential
	Transmembrane	180 – 200	21	Helical; Name=4; Potential
	Topological domain	201 – 232	32	Extracellular Potential
	Transmembrane	233 – 253	21	Helical; Name=5; Potential
	Topological domain	254 – 282	29	Cytoplasmic Potential
	Transmembrane	283 – 303	21	Helical; Name=6; Potential
	Topological domain	304 – 322	19	Extracellular Potential
	Transmembrane	323 – 343	21	Helical; Name=7; Potential
	Topological domain	344 – 393	50	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	11	1	N-linked (GlcNAc...) Potential
	Disulfide bond	137 ↔ 217		By similarity
Experimental info
	Sequence conflict	275 – 276	2	RL → TV in AAF43706. Ref.1
	Sequence conflict	275 – 276	2	RL → TV in AAM49570. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9JKL1 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: D0F5E79606D74283	FASTA	393	44,597
	10 20 30 40 50 60 METTVGALGE NTTDTFTDFF SALDGHEAQT GSLPFTFSYG DYDMPLDEEE DVTNSRTFFA 70 80 90 100 110 120 AKIVIGMALV GIMLVCGIGN FIFITALARY KKLRNLTNLL IANLAISDFL VAIVCCPFEM 130 140 150 160 170 180 DYYVVRQLSW EHGHVLCASV NYLRTVSLYV STNALLAIAI DRYLAIVHPL RPRMKCQTAA 190 200 210 220 230 240 GLIFLVWSVS ILIAIPAAYF TTETVLVIVE RQEKIFCGQI WPVDQQFYYR SYFLLVFGLE 250 260 270 280 290 300 FVGPVVAMTL CYARVSRELW FKAVPGFQTE QIRRRLRCRR RTVLGLVCVL SAYVLCWAPF 310 320 330 340 350 360 YGFTIVRDFF PSVFVKEKHY LTAFYVVECI AMSNSMINTL CFVTVRNNTS KYLKRILRLQ 370 380 390 WRASPSGSKA SADLDLRTTG IPATEEVDCI RLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Y-receptor-like genes GPR72 and GPR73: molecular cloning, genomic organisation and assignment to human chromosome 11q21.1 and 2p14 and mouse chromosome 9 and 6." Parker R., Liu M., Eyre H.J., Copeland N.G., Gilbert D.J., Crawford J., Sutherland G.R., Jenkins N.A., Herzog H. Biochim. Biophys. Acta 1491:369-375(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Prokineticin 2 transmits the behavioural circadian rhythm of the suprachiasmatic nucleus." Cheng M.Y., Bullock C.M., Li C., Lee A.G., Bermak J.C., Belluzzi J., Weaver D.R., Leslie F.M., Zhou Q.-Y. Nature 417:405-410(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Forelimb and Spleen.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF236082 mRNA. Translation: AAF43706.1. AF487278 mRNA. Translation: AAM49570.1. AK134279 mRNA. Translation: BAE22080.1. AK156734 mRNA. Translation: BAE33828.1. CH466523 Genomic DNA. Translation: EDK99210.1. BC059003 mRNA. Translation: AAH59003.1.
IPI	IPI00123133.
RefSeq	NP_067356.2. NM_021381.3.
UniGene	Mm.333226.
3D structure databases
ProteinModelPortal	Q9JKL1.
SMR	Q9JKL1. Positions 8-358.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000059034.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9JKL1.
Proteomic databases
PRIDE	Q9JKL1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000050887; ENSMUSP00000059034; ENSMUSG00000049409.
GeneID	58182.
KEGG	mmu:58182.
Organism-specific databases
CTD	10887.
MGI	MGI:1929676. Prokr1.
Phylogenomic databases
eggNOG	NOG319668.
GeneTree	ENSGT00670000098043.
HOGENOM	HOG000231664.
HOVERGEN	HBG039631.
InParanoid	Q3U0M4.
KO	K08379.
OMA	GIPATEE.
OrthoDB	EOG4SF966.
Gene expression databases
Bgee	Q9JKL1.
CleanEx	MM_PROKR1.
Genevestigator	Q9JKL1.
GermOnline	ENSMUSG00000049409. Mus musculus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR000611. NPY_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01012. NRPEPTIDEYR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1949486.
NextBio	314139.
SOURCE	Search...

Entry information

Entry name

PKR1_MOUSE

Accession

Primary (citable) accession number: Q9JKL1
Secondary accession number(s): Q3U0M4, Q6PD11

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program